LOCUS       pHB1                    3660 bp ds-DNA     circular     23-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(2329..2346)
                     /label="M13 Forward"
     primer_bind     1269..1285
                     /label="M13 rev"
     primer_bind     1250..1272
                     /label="M13/pUC Reverse"
     primer_bind     784..803
                     /label="pBR322ori-F"
     primer_bind     complement(2338..2360)
                     /label="M13/pUC Forward"
     misc_feature    1304..1335
                     /label="LL (left linker)"
     rep_origin      295..883
                     /label="ori"
     oligo           1482..1511
                     /label="MRH013"
     misc_feature    1336..1369
                     /label="FRT site"
     Oligo           complement(2221..2246)
                     /label="ErmR-RE"
     Oligo           1370..1405
                     /label="ErmR-FO"
     misc_feature    2281..2301
                     /label="Spacer"
     primer_bind     complement(2554..2573)
                     /label="pRS-marker"
     primer_bind     complement(2733..2751)
                     /label="pBRforEco"
     promoter        2819..2923
                     /label="AmpR promoter"
     primer_bind     complement(3142..3161)
                     /label="Amp-R"
     promoter        1207..1237
                     /label="lac promoter"
     primer_bind     2673..2695
                     /label="pGEX 3'"
     primer_bind     1037..1054
                     /label="L4440"
     misc_feature    1298..2328
                     /label="MCS"
     primer_bind     complement(2329..2345)
                     /label="M13 fwd"
     primer_bind     1269..1285
                     /label="M13 Reverse"
     protein_bind    1245..1261
                     /label="lac operator"
     CDS             1281..2578
                     /label="lacZ-alpha"
     CDS             1482..2219
                     /label="ErmR CDS"
     CDS             join(2924..3660,1..124)
                     /label="AmpR"
     misc_feature    1418..1433
                     /label="Putative promoter"
     misc_feature    2247..2280
                     /label="FRT site"
     oligo           complement(2196..2219)
                     /label="MRH014"
ORIGIN
        1 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
       61 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
      121 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
      181 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
      241 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      301 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      361 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      421 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
      481 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      541 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      601 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      661 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      721 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      781 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      841 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      901 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      961 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     1021 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     1081 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     1141 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     1201 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     1261 atttcacaca ggaaacagct atgaccatga ttacgccaag cttACGAGAC GAGCTTCTTA
     1321 TATATGCTTC GCCAGgaagt tcctattctc tagaaagtat aggaacttcC TTAGAAGCAA
     1381 ACTTAAGAGT GTGTTGATAG TGCAGTATCT TAAAATTTTG TATAATAGGA ATTGAAGTTA
     1441 AATTAGATGC TAAAAATTTG TAATTAAGAA GGAGTGATTA CATGAACAAA AATATAAAAT
     1501 ATTCTCAAAA CTTTTTAACG AGTGAAAAAG TACTCAACCA AATAATAAAA CAATTGAATT
     1561 TAAAAGAAAC CGATACCGTT TACGAAATTG GAACAGGTAA AGGGCATTTA ACGACGAAAC
     1621 TGGCTAAAAT AAGTAAACAG GTAACGTCTA TTGAATTAGA CAGTCATCTA TTCAACTTAT
     1681 CGTCAGAAAA ATTAAAACTG AATACTCGTG TCACTTTAAT TCACCAAGAT ATTCTACAGT
     1741 TTCAATTCCC TAACAAACAG AGGTATAAAA TTGTTGGGAG TATTCCTTAC CATTTAAGCA
     1801 CACAAATTAT TAAAAAAGTG GTTTTTGAAA GCCATGCGTC TGACATCTAT CTGATTGTTG
     1861 AAGAAGGATT CTACAAGCGT ACCTTGGATA TTCACCGAAC ACTAGGGTTG CTCTTGCACA
     1921 CTCAAGTCTC GATTCAGCAA TTGCTTAAGC TGCCAGCGGA ATGCTTTCAT CCTAAACCAA
     1981 AAGTAAACAG TGTCTTAATA AAACTTACCC GCCATACCAC AGATGTTCCA GATAAATATT
     2041 GGAAGCTATA TACGTACTTT GTTTCAAAAT GGGTCAATCG AGAATATCGT CAACTGTTTA
     2101 CTAAAAATCA GTTTCATCAA GCAATGAAAC ACGCCAAAGT AAACAATTTA AGTACCGTTA
     2161 CTTATGAGCA AGTATTGTCT ATTTTTAATA GTTATCTATT ATTTAACGGG AGGAAATAAt
     2221 CTAGAATGCG AGAGTAGGGA ACTGCCgaag ttcctattct ctagaaagta taggaacttc
     2281 AGCGCTCATG CACTTGATTC Cggatccccg ggtaccgagc tcgaattcac tggccgtcgt
     2341 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2401 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2461 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     2521 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2581 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     2641 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     2701 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     2761 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     2821 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2881 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2941 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3001 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3061 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3121 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3181 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3241 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3301 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3361 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3421 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3481 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3541 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3601 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
//