LOCUS       pDIV296_pAMA1-NAT_opt_PacI_Nt.BbvCI-correct      9273 bp    DNA     circular UNA 01-dec-2010
DEFINITION  AMA1 expression platform generated by uClone. 4 (5) fragments assembled: ori-ampR, ampR (PM), AMA1 (or AMA1-A + AMA1-B), P::U::T and argB.
FEATURES             Location/Qualifiers
     CDS             complement(1..859)
                     /cds_type="ORF"
                     /label="ampR"
     Palindrome      1358..4031
                     /label="AMA_Pal1"
     rep_origin      1359..7079
                     /note="for Aspergillus"
                     /label="AMA1"
     misc_feature    complement(4359..4388)
                     /feature_type="USER Primer"
                     /label="KBR087"
     misc_feature    4381..4409
                     /feature_type="USER Primer"
                     /label="KBR088"
     Palindrome      complement(4407..7080)
                     /label="AMA_Pal2"
     primer          complement(7079..7112)
                     /label="pAMA_bckbPacI_R"
     promoter        7142..7461
                     /label="PtrpC"
     gene            7470..8042
                     /label="NAT opt"
     terminator      8051..8490
                     /label="TtrpC"
     rep_origin      8505..9170
                     /label="ori"
ORIGIN
        1 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
       61 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
      121 GCGCTGCGAT GATACCGCGA GAACCACGCT CACCGGCTCC GGATTTATCA GCAATAAACC
      181 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
      241 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
      301 TTGTTGCCAT CGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
      361 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
      421 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
      481 TGGTTATGGC AGCGCTACAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
      541 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
      601 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
      661 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
      721 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
      781 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
      841 GGAAATGTTG AATACTCATA TTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
      901 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTCA
      961 GTGTTACAAC CAATTAACCA ATTCTGAACA TTATCGCGAG CCCATTTATA CCTGAATATG
     1021 GCTCATAACA CCCCTTGTTT GCCTGGCGGC AGTAGCGCGG TGGTCCCACC TGACCCCATG
     1081 CCGAACTCAG AAGTGAAACG CCGTAGCGCC GATGGTAGTG TGGGGACTCC CCATGCGAGA
     1141 GTAGGGAACT GCCAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG
     1201 CCCGGGCTAA TTATGGGGTG TCGCCCTTAT TCGACTCTAT AGTGAAGTTC CTATTCTCTA
     1261 GAAAGTATAG GAACTTCTGA AGTGGGGATT TAAATGCGGC CGCGCTGAGG GTTTAATCGA
     1321 CGAAGCAGCT GACGGCCAGT GCCAAGCTTA ACGCGTACCC GGGCCCAGTA TATGTTCCGC
     1381 AGATGACTGG AGCTCTGCCA TACGTGCCCT CTCAAGCACC ATTTGTTCCA TCTACAGAGA
     1441 CTAGTCACCA ACTAGTCTAT CAAGACTCAC AGGGTACATT GCTGAGACCA ACTGACCAGA
     1501 GGCAGGGTAG CGGATTGACG GCTCCATCTC CTTCACTTAC AAGGTCTATT GAAAGCCCTT
     1561 TAGCATCACC AAGCGGAGAA TAGATTGTTA AGCTTATTTT TTGTATACTG TTTTGTGATA
     1621 GCACGAAGTT TTTCCACGGT ATCTTGTAAA AATATATATT TGTGGCGGGC TTACCTACAT
     1681 CAAATTAATA AGAGACTAAT TATAAACTAA ACACACAAGC AAGCTACTTT AGGGTAAAAG
     1741 TTTATAAATG CTTTTGACGT ATAAACGTTG CTTGTATTTA TTATTACAAT TAAAGGTGGA
     1801 TAGAAAACCT AGAGACTAGT TAGAAACTAA TCTCAGGTTT GCGTTAAACT AAATCAGAGC
     1861 CCGAGAGGTT AACAGAACCT AGAAGGGGAC TAGATATCCG GGTAGGGAAA CAAAAAAAAA
     1921 AAACAAGACA GCCACATATT AGGGAGACTA GTTAGAAGCT AGTTCCAGGA CTAGGAAAAT
     1981 AAAAGACAAT GATACCACAG TCTAGTTGAC AACTAGATAG ATTCTAGATT GAGGCCAAAG
     2041 TCTCTGAGAT CCAGGTTAGT TGCAACTAAT ACTAGTTAGT ATCTAGTCTC CTATAACTCT
     2101 GAAGCTAGAA TAACTTACTA CTATTATCCT CACCACTGTT CAGCTGCGCA AACGGAGTGA
     2161 TTGCAAGGTG TTCAGAGACT AGTTATTGAC TAGTCAGTGA CTAGCAATAA CTAACAAGGT
     2221 ATTAACCTAC CATGTCTGCC ATCACCCTGC ACTTCCTCGG GCTCAGCAGC CTTTTCCTCC
     2281 TCATTTTCAT GCTCATTTTC CTTGTTTAAG ACTGTGACTA GTCAAAGACT AGTCCAGAAC
     2341 CACAAAGGAG AAATGTCTTA CCACTTTCTT CATTGCTTGT CTCTTTTGCA TTATCCATGT
     2401 CTGCAACTAG TTAGAGTCTA GTTAGTGACT AGTCCGACGA GGACTTGCTT GTCTCCGGAT
     2461 TGTTGGAGGA ACTCTCCAGG GCCTCAAGAT CCACAACAGA GCCTTCTAGA AGACTGGTCA
     2521 ATAACTAGTT GGTCTTTGTC TGAGTCTGAC TTACGAGGTT GCATACTCGC TCCCTTTGCC
     2581 TCGTCAATCG ATGAGAAAAA GCGCCAAAAC TCGCAATATG GCTTTGAACC ACACGGTGCT
     2641 GAGACTAGTT AGAATCTAGT CCCAAACTAG CTTGGATAGC TTACCTTTGC CCTTTGCGTT
     2701 GCGACAGGTC TTGCAGGGTA TGGTTCCTTT CTCACCAGCT GATTTAGCTG CCTTGCTACC
     2761 CTCACGGCGG ATCTGCCATA AAGAGTGGCT AGAGGTTATA AATTAGCACT GATCCTAGGT
     2821 ACGGGGCTGA ATGTAACTTG CCTTTCCTTT CTCATCGCGC GGCAAGACAG GCTTGCTCAA
     2881 ATTCCTACCA GTCACAGGGG TATGCACGGC GTACGGACCA CTTGAACTAG TCACAGATTA
     2941 GTTAGCAACT AGTCTGCATT GAATGGCTGT ACTTACGGGC CCTCGCCATT GTCCTGATCA
     3001 TTTCCAGCTT CACCCTCGTT GCTGCAAAGT AGTTAGTGAC TAGTCAAGGA CTAGTTGAAA
     3061 TGGGAGAAGA AACTCACGAA TTCTCGACTC CCTTAGTATT GTGGTCCTTG GACTTGGTGC
     3121 TGCTATATAT TAGCTAATAC ACTAGTTAGA CTCACAGAAA CTTACGCAGC TCGCTTGCGC
     3181 TTCTTGGTAG GAGTCGGGGT TGGGAGAACA GTGCCTTCAA ACAAGCCTTC ATACCATGCT
     3241 ACTTGACTAG TCAGGGACTA GTCACCAAGT AATCTAGATA GGACTTGCCT TTGGCCTCCA
     3301 TCAGTTCCTT CATAGTGGGA GGACCATTGT GCAATGTAAA CTCCATGCCG TGGGAGTTCT
     3361 TGTCCTTCAA GTGCTTGACC AATATGTTTC TGTTGGCAGA GGGAACCTGT CAACTAGTTA
     3421 ATAACTAGTC AGAAACTATG ATAGCAGTAG ACTCACTGTA CGCTTGAGGC ATCCCTTCAC
     3481 TCGGCAGTAG ACTTCATATG GATGGATATC AGGCACGCCA TTGTCGTCCT GTGGACTAGT
     3541 CAGTAACTAG GCTTAAAGCT AGTCGGGTCG GCTTACTATC TTGAAATCCG GCAGCGTAAG
     3601 CTCCCCGTCC TTAACTGCCT CGAGATAGTG ACAGTACTCT GGGGACTTTC GGAGATCGTT
     3661 ATCGTTATCG CGAATGCTCG GCATACTAAC TGTTGACTAG TCTTGGACTA GTCCCGAGCA
     3721 AAAAGGATTG GAGGAGGAGG AGGAAGGTGA GAGTGAGACA AAGAGCGAAA TAAGAGCTTC
     3781 AAAGGCTATC TCTAAGCAGT ATGAAGGTTA AGTATCTAGT TCTTGACTAG ATTTAAAGAG
     3841 ATTTCGACTA GTTATGTACC TGGAGTTTGG ATATAGGAAT GTGTTGTGGT AACGAAATGT
     3901 AAGGGGGAGG AAAGAAAAAG TCGTCAAGAG GTAACTCTAA GTCGGCCATT CCTTTTTGGG
     3961 AGGCGCTAAC CATAAACGGC ATGGTCGACT TAGAGTTAGC TCAGGGAATT TAGGGAGTTA
     4021 TCTGCGACCA CCGAGGAACG GCGGAATGCC AAAGAATCCC GATGGAGCTC TAGCTGGCGG
     4081 TTGACAACCC CACCTTTTGG CGTTTCTGCG GCGTTGCAGG CGGGACTGGA TACTTCGTAG
     4141 AACCAGAAAG GCAAGGCAGA ACGCGCTCAG CAAGAGTGTT GGAAGTGATA GCATGATGTG
     4201 CCTTGTTAAC TAGGTACCAA TCTGCAGTAT GCTTGATGTT ATCCAAAGTG TGAGAGAGGA
     4261 AGGTCCAAAC ATACACGATT GGGAGAGGGC CTAGGTATAA GAGTTTTTGA GTAGAACGCA
     4321 TGTGAGCCCA GCCATCTCGA GGAGATTAAA CACGGGCCGG CATTTGATGG CTATGTTAGT
     4381 ACCCCAATGG AAACGGTGAG AGTCCAGTGG TCGCAGATAA CTCCCTAAAT TCCCTGAGCT
     4441 AACTCTAAGT CGACCATGCC GTTTATGGTT AGCGCCTCCC AAAAAGGAAT GGCCGACTTA
     4501 GAGTTACCTC TTGACGACTT TTTCTTTCCT CCCCCTTACA TTTCGTTACC ACAACACATT
     4561 CCTATATCCA AACTCCAGGT ACATAACTAG TCGAAATCTC TTTAAATCTA GTCAAGAACT
     4621 AGATACTTAA CCTTCATACT GCTTAGAGAT AGCCTTTGAA GCTCTTATTT CGCTCTTTGT
     4681 CTCACTCTCA CCTTCCTCCT CCTCCTCCAA TCCTTTTTGC TCGGGACTAG TCCAAGACTA
     4741 GTCAACAGTT AGTATGCCGA GCATTCGCGA TAACGATAAC GATCTCCGAA AGTCCCCAGA
     4801 GTACTGTCAC TATCTCGAGG CAGTTAAGGA CGGGGAGCTT ACGCTGCCGG ATTTCAAGAT
     4861 AGTAAGCCGA CCCGACTAGC TTTAAGCCTA GTTACTGACT AGTCCACAGG ACGACAATGG
     4921 CGTGCCTGAT ATCCATCCAT ATGAAGTCTA CTGCCGAGTG AAGGGATGCC TCAAGCGTAC
     4981 AGTGAGTCTA CTGCTATCAT AGTTTCTGAC TAGTTATTAA CTAGTTGACA GGTTCCCTCT
     5041 GCCAACAGAA ACATATTGGT CAAGCACTTG AAGGACAAGA ACTCCCACGG CATGGAGTTT
     5101 ACATTGCACA ATGGTCCTCC CACTATGAAG GAACTGATGG AGGCCAAAGG CAAGTCCTAT
     5161 CTAGATTACT TGGTGACTAG TCCCTGACTA GTCAAGTAGC ATGGTATGAA GGCTTGTTTG
     5221 AAGGCACTGT TCTCCCAACC CCGACTCCTA CCAAGAAGCG CAAGCGAGCT GCGTAAGTTT
     5281 CTGTGAGTCT AACTAGTGTA TTAGCTAATA TATAGCAGCA CCAAGTCCAA GGACCACAAT
     5341 ACTAAGGGAG TCGAGAATTC GTGAGTTTCT TCTCCCATTT CAACTAGTCC TTGACTAGTC
     5401 ACTAACTACT TTGCAGCAAC GAGGGTGAAG CTGGAAATGA TCAGGACAAT GGCGAGGGCC
     5461 CGTAAGTACA GCCATTCAAT GCAGACTAGT TGCTAACTAA TCTGTGACTA GTTCAAGTGG
     5521 TCCGTACGCC GTGCATACCC CTGTGACTGG TAGGAATTTG AGCAAGCCTG TCTTGCCGCG
     5581 CGATGAGAAA GGAAAGGCAA GTTACATTCA GCCCCGTACC TAGGATCAGT GCTAATTTAT
     5641 AACCTCTAGC CACTCTTTAT GGCAGATCCG CCGTGAGGGT AGCAAGGCAG CTAAATCAGC
     5701 TGGTGAGAAA GGAACCATAC CCTGCAAGAC CTGTCGCAAC GCAAAGGGCA AAGGTAAGCT
     5761 ATCCAAGCTA GTTTGGGACT AGATTCTAAC TAGTCTCAGC ACCGTGTGGT TCAAAGCCAT
     5821 ATTGCGAGTT TTGGCGCTTT TTCTCATCGA TTGACGAGGC AAAGGGAGCG AGTATGCAAC
     5881 CTCGTAAGTC AGACTCAGAC AAAGACCAAC TAGTTATTGA CCAGTCTTCT AGAAGGCTCT
     5941 GTTGTGGATC TTGAGGCCCT GGAGAGTTCC TCCAACAATC CGGAGACAAG CAAGTCCTCG
     6001 TCGGACTAGT CACTAACTAG ACTCTAACTA GTTGCAGACA TGGATAATGC AAAAGAGACA
     6061 AGCAATGAAG AAAGTGGTAA GACATTTCTC CTTTGTGGTT CTGGACTAGT CTTTGACTAG
     6121 TCACAGTCTT AAACAAGGAA AATGAGCATG AAAATGAGGA GGAAAAGGCT GCTGAGCCCG
     6181 AGGAAGTGCA GGGTGATGGC AGACATGGTA GGTTAATACC TTGTTAGTTA TTGCTAGTCA
     6241 CTGACTAGTC AATAACTAGT CTCTGAACAC CTTGCAATCA CTCCGTTTGC GCAGCTGAAC
     6301 AGTGGTGAGG ATAATAGTAG TAAGTTATTC TAGCTTCAGA GTTATAGGAG ACTAGATACT
     6361 AACTAGTATT AGTTGCAACT AACCTGGATC TCAGAGACTT TGGCCTCAAT CTAGAATCTA
     6421 TCTAGTTGTC AACTAGACTG TGGTATCATT GTCTTTTATT TTCCTAGTCC TGGAACTAGC
     6481 TTCTAACTAG TCTCCCTAAT ATGTGGCTGT CTTGTTTTTT TTTTTTGTTT CCCTACCCGG
     6541 ATATCTAGTC CCCTTCTAGG TTCTGTTAAC CTCTCGGGCT CTGATTTAGT TTAACGCAAA
     6601 CCTGAGATTA GTTTCTAACT AGTCTCTAGG TTTTCTATCC ACCTTTAATT GTAATAATAA
     6661 ATACAAGCAA CGTTTATACG TCAAAAGCAT TTATAAACTT TTACCCTAAA GTAGCTTGCT
     6721 TGTGTGTTTA GTTTATAATT AGTCTCTTAT TAATTTGATG TAGGTAAGCC CGCCACAAAT
     6781 ATATATTTTT ACAAGATACC GTGGAAAAAC TTCGTGCTAT CACAAAACAG TATACAAAAA
     6841 ATAAGCTTAA CAATCTATTC TCCGCTTGGT GATGCTAAAG GGCTTTCAAT AGACCTTGTA
     6901 AGTGAAGGAG ATGGAGCCGT CAATCCGCTA CCCTGCCTCT GGTCAGTTGG TCTCAGCAAT
     6961 GTACCCTGTG AGTCTTGATA GACTAGTTGG TGACTAGTCT CTGTAGATGG AACAAATGGT
     7021 GCTTGAGAGG GCACGTATGG CAGAGCTCCA GTCATCTGCG GAACATATAC TGGGCCCGGG
     7081 AAGATCTCAT GGTCATAGCT GGCTGAGGGT TTAATGCTGA GGGTTTAATT AAGACCTCAG
     7141 CGCTAGTGGA GGTCAACACA TCAATGCTAT TTTGGTTTAG TCGTCCAGGC GGATCACAAA
     7201 ATTTGTGTCG TTTGACAAGA TGGTTCATTT AGGCAACTGG TCAGATCAGC CCACTTGTAA
     7261 GCAGTAGCGG CGGCGCTCGA AGTGTGACTC TTATTAGCAG ACAGGAACGA GGACATTATT
     7321 ATCATCTGCT GCTTGGTGCA CGATAACTTG TGCGTTTGTC AAGCAAGGTA AGTGAACGAC
     7381 CCGGTCATAC CTTCTTAAGT TCGCCCTTCC TCCCTTTATT TCAGATTCAA TCTGACTTAC
     7441 CTATTCTACC CAAGCATCCA AAAACAACCA TGGGAACCAC CTTGGATGAC ACTGCTTATC
     7501 GATACCGAAC ATCTGTACCT GGTGATGCTG AAGCTATCGA AGCCCTTGAC GGGTCATTCA
     7561 CAACTGACAC CGTCTTTAGA GTTACAGCTA CTGGGGATGG GTTTACATTG CGAGAAGTAC
     7621 CAGTAGATCC TCCTTTGACC AAAGTATTCC CAGATGACGA AAGTGACGAT GAATCCGATG
     7681 ACGGTGAGGA CGGTGACCCA GACTCAAGAA CATTTGTCGC TTATGGAGAC GACGGTGATT
     7741 TGGCCGGTTT CGTAGTTGTA TCCTATTCTG GATGGAACCG AAGACTTACT GTCGAGGATA
     7801 TAGAGGTTGC CCCAGAACAT CGTGGGCACG GTGTTGGGCG AGCCCTTATG GGATTAGCTA
     7861 CAGAATTTGC CCGTGAACGT GGGGCTGGAC ATTTATGGTT AGAAGTCACC AATGTTAATG
     7921 CACCAGCTAT ACATGCATAT CGAAGAATGG GGTTCACACT TTGTGGATTG GATACAGCTT
     7981 TATACGATGG TACAGCCTCA GACGGAGAAC AAGCCTTATA CATGTCTATG CCTTGTCCAT
     8041 AATCAGTACT CCGACGCCGA CCAACACCGC CGGTCCGACG GCGGCCCACG GGTCCCAGGA
     8101 GCTTGAGATC CACTTAACGT TACTGAAATC ATCAAACAGC TTGACGAATC TGGATATAAG
     8161 ATCGTTGGTG TCGATGTCAG CTCCGGAGTT GAGACAAATG GTGTTCAGGA TCTCGATAAG
     8221 ATACGTTCAT TTGTCCAAGC AGCAAAGAGT GCCTTCTAGT GATTTAATAG CTCCATGTCA
     8281 ACAAGAATAA AACGCGTTTT CGGGTTTACC TCTTCCAGAT ACAGCTCATC TGCAATGCAT
     8341 TAATGCATTG ACTGCAACCT AGTAACGCCT TNCAGGCTCC GGCGAAGAGA AGAATAGCTT
     8401 AGCAGAGCTA TTTTCATTTT CGGGAGACGA GATCAAGCAG ATCAACGGTC GTCAAGAGAC
     8461 CTACGAGACT GAGGAATCCG CTCTTGGCTC ATTAAGACCT CAGCGAGCAA AAGGCCAGCA
     8521 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC
     8581 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     8641 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     8701 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     8761 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     8821 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     8881 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     8941 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGGCT AACTACGGCT ACACTAGAAG
     9001 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     9061 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     9121 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     9181 CGCTCAGTGG AACGACGCGC GCGTAACTCA CGTTAAGGGA TTTTGGTCAT GAGCTTGCGC
     9241 CGTCCCGTCA AGTCAGCGTA ATGCTCTGCT TTT
//
