LOCUS       Exported               15784 bp ds-DNA     circular SYN 24-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 26, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15784
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4388..5410
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          6750..6778
                     /label=source
     source          6779..14299
                     /label=source
     source          8675..8732
                     /label=source
     source          9644..9652
                     /label=source
     source          9653..9952
                     /label=source
     source          14225..14293
                     /label=Donor sequence
     source          14306..15013
                     /label=Donor sequence
     misc_feature    1..1200
                     /label=from K44 3'ITRplus
     repeat_region   274..308
                     /label=3'ITR
     misc_feature    complement(293..295)
                     /label=3bp spacer
                     /note="ApEinfo_revcolor=#0000a0
                     ApEinfo_fwdcolor=#ff80ff"
     primer_bind     complement(799..815)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    823..839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(827..846)
                     /label=pLac(F)
                     /note="ApEinfo_revcolor=#19ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#5381ff"
     promoter        complement(847..877)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    892..913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1207..1913)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_revcolor=pink"
     CDS             complement(2011..2670)
                     /label=AmpR
                     /note="ApEinfo_fwdcolor=#67ff51
                     ApEinfo_revcolor=#67ff51"
     misc_feature    complement(2910..2938)
                     /label=AmpR promoter
                     /note="ApEinfo_fwdcolor=#ffffff
                     ApEinfo_revcolor=#f06bff"
     misc_feature    3252..3776
                     /label=from K44 5'ITRplus
     primer_bind     3393..3409
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3477..3496
                     /label=BII(R)
                     /note="ApEinfo_revcolor=#19ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#5381ff"
     repeat_region   join(3539..3551,3583..3584,3586..3601)
                     /label=5'TR
     misc_feature    3552..3582
                     /label=31bp spacer
                     /note="ApEinfo_revcolor=#ff77e0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff0080"
     repeat_region   3552..3582
                     /label=5'ITR
     misc_feature    3777..4376
                     /label=wPRE
                     /note="ApEinfo_fwdcolor=#ffffff
                     ApEinfo_revcolor=#f06bff"
     misc_feature    3777..4376
                     /label=insert from L50
     misc_feature    4388..5437
                     /label=iAA61 hygro_var
                     /note="cut bb AsiSI and NheI"
     CDS             complement(4388..5410)
                     /codon_start=1
                     /label=Hygro
                     /label=Hygro (NoCpG, codon-optimized)
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          5431..6369
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     promoter        complement(6370..6581)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    6588..6741
                     /label=Transcription blocker
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene combined with a synthetic 
                     polyA signal"
     misc_feature    6588..6741
                     /label=sequence from pTetOne vector
     primer_bind     complement(6761..6778)
                     /label=BGH-rev
     polyA_signal    6767..6778
                     /label=bGH poly(A) signal
     misc_feature    join(6770..15784,1..3756)
                     /label=211013 iB22.B
     polyA_signal    6779..6991
                     /label=bGH poly(A) signal
     enhancer        6997..7376
                     /label=CMV enhancer
     promoter        7378..7655
                     /label=chicken beta-actin promoter
     intron          7657..8674
                     /label=chimeric intron
     misc_feature    8675..8732
                     /label=211013 iB22.B
     misc_feature    8708..8732
                     /label=Q5 Tm 67
     misc_feature    8733..9637
                     /label=lox511-STOP-lox511
     protein_bind    complement(8733..8766)
                     /label=lox511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    8933..9067
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    9187..9321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    9441..9575
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(9604..9637)
                     /label=lox511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      9644..9652
                     /regulatory_class="other"
                     /label=Kozak
                     /label=vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)
     misc_feature    9649..9652
                     /label=Q5 tm 67
     misc_feature    9653..9952
                     /label=from pJB69
     CDS             9653..9952
                     /codon_start=1
                     /label=ZIM3 (1-100)
                     /translation="MNNSQGRVTFEDVTVNFTQGEWQRLNPEQRNLYRDVMLENYSNLV
                     SVGQGETTKPDVILRLEQGKEPWLEEEEVLGSGRAEKNGDIGGQIWKPKDVKESL"
     misc_feature    9653..9667
                     /label=Q5 tm 67
     misc_feature    9953..14299
                     /label=from pJB69
     CDS             9959..14062
                     /label=dCas9
     CDS             14066..14092
                     /label=HA
     CDS             14111..14131
                     /label=SV40 NLS
     misc_feature    complement(14129..14158)
                     /label=Tm 71.1˚C
     CDS             14138..14158
                     /label=SV40 NLS
     misc_feature    14148..14305
                     /label=iAA53 zim3_p2a_mapple_gb
     misc_feature    14166..14167
                     /label=to keep 2?
     CDS             14168..14224
                     /codon_start=1
                     /label=P2A
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    14205..14224
                     /label=Gibson overhang
     misc_feature    14207..14224
                     /label=Q5 Tm 66
     misc_signal     14225..14293
                     /label=SeqVer (AAV F)
     misc_feature    14225..14251
                     /label=mycNLS
     misc_feature    14267..14293
                     /label=mycNLS
     misc_signal     14306..15013
                     /label=SeqVer (AAV F)
     CDS             14306..15013
                     /codon_start=1
                     /label=Optimized mApple
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    14306..15013
                     /label=IF-1
                     /note="from iA28"
     misc_feature    15017..15028
                     /label=insert
     misc_feature    join(15038..15784,1..3776)
                     /label=insert from L50
     misc_feature    15038..15637
                     /label=wPRE
                     /note="ApEinfo_fwdcolor=#ffffff
                     ApEinfo_revcolor=#f06bff"
ORIGIN
        1 gattctataa caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa
       61 caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat
      121 tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg
      181 cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc
      241 tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct
      301 ttctagggtt aatctagctg catcaggatc atatcgtcgg gtcttttttc cggctcagtc
      361 atcgcccaag ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg
      421 aggttgaagc ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc
      481 gaaaacgcac gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg
      541 aactgttcgt tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc
      601 ggatggtcag cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc
      661 gtgccggtgt gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg
      721 ggtatcctgg ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc
      781 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      841 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      901 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      961 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1201 ggatccggcc gcgttgctgg cgttggatcc ggccgcgttg ctggcgtttt tccataggct
     1261 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1321 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1381 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1441 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1501 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1561 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1621 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1681 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1741 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1861 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1921 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1981 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2041 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2101 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2161 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2221 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2281 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2341 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2401 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2461 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2521 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2581 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2641 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2701 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2761 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2821 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2881 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2941 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3001 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     3061 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     3121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     3181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     3241 cattgattat tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     3301 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     3361 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcctc
     3421 gttcattcac gtttttgaac ccgtggagga cgggcagact cgcggtgcaa atgtgtttta
     3481 cagcgtgatg gagcagatga agatgctcga cacgctgcag aacacgcagc tagattaacc
     3541 ctagaaagat aatcatattg tgacgtacgt taaagataat catgtgtaaa attgacgcat
     3601 gtgttttatc ggtctgtata tcgaggttta tttattaatt tgaatagata ttaagtttta
     3661 ttatatttac acttacatac taataataaa ttcaacaaac aatttattta tgtttattta
     3721 tttattaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttatatgc
     3781 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
     3841 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     3901 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga
     3961 aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     4021 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     4081 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     4141 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
     4201 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
     4261 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     4321 agaataccag tcaatctttc acaaattttg taatccagag gttgattatc gataaggttt
     4381 aaacttattc cttggctctg ggtcttgtgg agggtctcct gtttccagag tcagccagaa
     4441 cttcaacaca tccatcagtc caaacagcag cagaccttct tgcaatttga gttcttccaa
     4501 cagttccagc accagacctc acaatggcat cacatcttcc ttgtgcccaa gcagcatcat
     4561 caaagtttcc atcaaccaga gattgataca gttggtccag gccaattctg agcatgtagg
     4621 ctctcagtct gggggaacca gccagttctg ggtgtcttct ttcaaaatat cttgtttgtt
     4681 gttccatgca agccagccaa ggtctccaaa aaaaaatgtt ggcaacctca tattgagaat
     4741 ctccaaacat ggcttcagac cagtcaatga ctgcagtgat tctgccattg tctgtcagaa
     4801 cattgttgct tccaaaatca gcatggacca ggtgtctgac ttcaggacaa tcttctgccc
     4861 acagcatgag ttcatccagt gcctgagcaa cagaagcaga aactgtgtca tccatcacag
     4921 tctgccagtg atagacatga ggatcagcaa tggcacaaat gaaatccctc caagtggtgt
     4981 actgaccgat gccttgggga ccaaaaggac caaatccgct ggtttggctc agatcagctg
     5041 ctgcaatggc atccattgct tcagcaacag gttgcagaac agctggcagc tcagtttcag
     5101 ggagatcctg gagagtgact ccttgtgctc ttctgctgat gcagtaggtg agagattcag
     5161 aaaattctcc aatgtccaga acttctggaa ttggcagagc agcagaggca aagtgtctgt
     5221 aaacatatct gtctttgtaa aaaccatcag cacaagaatt gaccctcaga acataacctc
     5281 ttcctccaac atcaaaagaa aaggctctgc tttcttcacc ttcagacagc tgcatgagat
     5341 cagaaacaga atcaaatttt tcaatgagaa acttctcaac agaagttgct gtcagttcag
     5401 gtttcttcat ggtgggctag ctcacgacac ctgaaatgga agaaaaaaac tttgaaccac
     5461 tgtctgaggc ttgagaatga accaagatcc aaactcaaaa agggcaaatt ccaaggagaa
     5521 ttacatcaag tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc
     5581 catcgcataa aacccctccc cccaacctaa agacgacgta ctccaaaagc gcgagaacta
     5641 atcgaggtgc ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga
     5701 cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt
     5761 cctccatttt gagctccctg cagcagggcc gggaagcggc catctttccg ctcacgcaac
     5821 tggtgccgac cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgaggcc
     5881 aggcaccaga gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc
     5941 tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc
     6001 ggccccaaaa accgaaatac cagtgtgcag atcttggccc gcatttacaa gactatcttg
     6061 ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa atggctagag acttatcgaa
     6121 agcagcgaga caggcgcgaa ggtgccacca gattcgcacg cggcggcccc agcgcccagg
     6181 ccaggcctca actcaagcac gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc
     6241 ccacccactt ccaacccgaa gctcgggatc aagaatcacg tactgcagcc aggtggaagt
     6301 aattcaaggc acgcaagggc cataacccgt aaagaggcca ggcccgcggg aaccacacac
     6361 ggcacttacc tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca cggcgactac
     6421 tgcacttata tacggttctc ccccaccctc gggaaaaagg cggagccagt acacgacatc
     6481 actttcccag tttaccccgc gccaccttct ctaggcaccg gttcaattgc cgacccctcc
     6541 ccccaacttc tcggggactg tgggcgatgt gcgctctgcc cttcgaaaat aaaatatctt
     6601 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     6661 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     6721 caggtgccag aacatttctc tttaattaac cgctgatcag cctcgactgt gccttctagt
     6781 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     6841 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     6901 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     6961 aggcatgctg gggatgcggt gggctctatg ggtctcgaca ttgattattg actagttatt
     7021 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     7081 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     7141 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     7201 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     7261 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     7321 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
     7381 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     7441 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc
     7501 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
     7561 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     7621 ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac gctgccttcg
     7681 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     7741 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     7801 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
     7861 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     7921 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     7981 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     8041 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     8101 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     8161 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     8221 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     8281 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
     8341 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     8401 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
     8461 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     8521 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
     8581 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     8641 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     8701 gctgtctcat cattttggca aagaatgtcg acataacttc gtataatgta tactatacga
     8761 agttattcgc gatgaataaa tgaaagcttg cagatctgcg accctagagg atctgcgacc
     8821 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     8881 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     8941 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9001 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9061 ctggatctgc gaccctagag gatcataatc agccatacca catttgtaga ggttttactt
     9121 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     9181 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9241 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9301 gtatcttatc atgtctggat ctgcgaccct agaggatcat aatcagccat accacatttg
     9361 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaactaaaaa
     9421 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     9481 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9541 ccaaactcat caatgtatct tatcatgtct ggatctccat caagctgatc cggaaccctt
     9601 aatataactt cgtataatgt atactatacg aagttatggt accgccgcca ccatgaacaa
     9661 ctctcagggc agagtgacct tcgaggacgt gaccgtgaac ttcacccaag gcgagtggca
     9721 gagactgaac cccgagcaga gaaacctgta ccgggacgtg atgctggaaa actacagcaa
     9781 cctggtgtcc gtcggccagg gcgagacaac aaagcctgac gtgatcctgc ggctggaaca
     9841 gggcaaagag ccttggctgg aagaggaaga ggtgctcgga tctggcagag ccgagaagaa
     9901 tggcgatatc ggcggacaga tctggaagcc caaggacgtg aaagagtctc tgcctaggat
     9961 ggacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat
    10021 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
    10081 cagcatcaag aagaacctga tcggcgccct gctgttcgac agcggagaaa cagccgaggc
    10141 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
    10201 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
    10261 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
    10321 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
    10381 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
    10441 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
    10501 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
    10561 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
    10621 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggcaacct
    10681 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
    10741 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
    10801 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
    10861 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
    10921 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
    10981 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
    11041 ctacatcgat ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
    11101 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa
    11161 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
    11221 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
    11281 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
    11341 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
    11401 ggtggacaag ggcgccagcg cccagagctt catcgagcgg atgaccaact tcgataagaa
    11461 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
    11521 caacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
    11581 cggcgagcag aaaaaagcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
    11641 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
    11701 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
    11761 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
    11821 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
    11881 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
    11941 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
    12001 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
    12061 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
    12121 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
    12181 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
    12241 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
    12301 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
    12361 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
    12421 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggacgctat
    12481 cgtgcctcag agctttctga aggacgactc catcgataac aaagtgctga ctcggagcga
    12541 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
    12601 ctactggcgc cagctgctga atgccaagct gattacccag aggaagttcg acaatctgac
    12661 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
    12721 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
    12781 taagtacgac gagaacgaca aactgatccg ggaagtgaaa gtgatcaccc tgaagtccaa
    12841 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
    12901 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
    12961 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
    13021 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
    13081 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
    13141 tctgatcgag acaaacggcg aaacaggcga gatcgtgtgg gataagggcc gggactttgc
    13201 caccgtgcgg aaagtgctgt ctatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
    13261 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgaca agctgatcgc
    13321 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta
    13381 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
    13441 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
    13501 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
    13561 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca
    13621 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
    13681 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
    13741 gcacaaacac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
    13801 cctggccgac gctaatctgg acaaggtgct gagcgcctac aacaagcaca gagacaagcc
    13861 tatcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
    13921 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga
    13981 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
    14041 cctgtctcag ctgggaggcg acgcctatcc ctatgacgtg cccgattatg ccagcctggg
    14101 cagcggctcc cccaagaaaa aacgcaaggt ggaagatcct aagaaaaagc ggaaagtgag
    14161 cggccgcgct actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg
    14221 acctcctgcc gctaaacggg tcaaactcga cggcggttct ggaggtcctg ctgcaaagcg
    14281 cgttaagttg gatctcgaga ccggtatggt gtccaagggc gaagagaaca acatggccat
    14341 catcaaagaa ttcatgcggt tcaaggtgca catggaaggc agcgtgaacg gccacgagtt
    14401 cgagattgaa ggcgaaggcg agggcagacc ctacgaggct tttcagaccg ccaagctgaa
    14461 agtgaccaaa ggcggccctc tgccttttgc ctgggacatt ctgagccctc agtttatgta
    14521 cggcagcaag gtgtacatca agcaccccgc cgacattccc gactacttca agctgagctt
    14581 ccccgagggc ttcagatggg agagagtgat gaacttcgag gacggcggca tcatccacgt
    14641 gaaccaggat agctctctgc aggacggcgt gttcatctac aaagtgaagc tgcggggcac
    14701 caactttccc tctgatggcc ccgtgatgca gaaaaagacc atgggctggg aagccagcga
    14761 ggaaagaatg taccctgagg atggcgccct gaagtccgag atcaagaagc ggctgaagct
    14821 gaaggatggc ggccactatg ccgccgaagt gaaaaccacc tacaaggcca agaaacccgt
    14881 gcagctgcct ggcgcctaca tcgtggatat caagctggac atcgtgtccc acaacgagga
    14941 ctacaccatc gtggaacagt acgagagagc cgaaggcaga cacagcacag gcggaatgga
    15001 cgagctgtac aagtaagcta gcttataaga attcaagctt atcgataatc aacctctgga
    15061 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
    15121 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
    15181 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
    15241 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
    15301 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
    15361 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
    15421 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
    15481 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
    15541 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
    15601 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag
    15661 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
    15721 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
    15781 taag
//
