LOCUS       Exported               17971 bp ds-DNA     circular SYN 21-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17971)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 26, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17971
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1748..6502
                     /label=source
     source          6371..6376
                     /label=source
     source          6377..6379
                     /label=source
     source          6434..6502
                     /label=Donor sequence
     misc_feature    9..389
                     /label=CMV enhancer
     enhancer        390..1635
                     /label=CAG
                     /note="Common feature added by Mo Mandegar"
     intron          1643..1735
                     /label=Mini-Intron
                     /note="Common feature added by Mo Mandegar
                     "
     regulatory      1748..1756
                     /regulatory_class="other"
                     /label=Kozak
                     /label=vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)
     misc_feature    1753..1771
                     /label=Q5 tm 67
     misc_feature    1757..6502
                     /label=from pJB69
     CDS             1757..2056
                     /codon_start=1
                     /label=ZIM3 (1-100)
                     /translation="MNNSQGRVTFEDVTVNFTQGEWQRLNPEQRNLYRDVMLENYSNLV
                     SVGQGETTKPDVILRLEQGKEPWLEEEEVLGSGRAEKNGDIGGQIWKPKDVKESL"
     CDS             2057..2152
                     /codon_start=1
                     /label=XTENsion
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             2159..6262
                     /label=dCas9
     misc_feature    6272..6502
                     /label=211013 iB22.B
     CDS             6272..6298
                     /label=HA
     CDS             6317..6337
                     /label=SV40 NLS
     misc_feature    complement(6335..6364)
                     /label=Tm 71.1˚C
     CDS             6344..6364
                     /label=SV40 NLS
     misc_feature    6354..6502
                     /label=iAA53 zim3_p2a_mapple_gb
     misc_feature    6371..6376
                     /label=from pJB69
     misc_feature    6371..6376
                     /label=iAA53 zim3_p2a_mapple_gb
     misc_feature    6371..6376
                     /label=211013 iB22.B
     misc_feature    6377..6379
                     /label=from pJB69
     misc_feature    6377..6379
                     /label=iAA53 zim3_p2a_mapple_gb
     misc_feature    6377..6379
                     /label=211013 iB22.B
     CDS             6380..6433
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_signal     6434..6502
                     /label=SeqVer (AAV F)
     misc_feature    6434..6460
                     /label=mycNLS
     misc_feature    6476..6502
                     /label=mycNLS
     misc_feature    6509..7210
                     /label=211013 iB22.B
     CDS             6509..7210
                     /codon_start=1
                     /label=mScarlet
                     /translation="MEFVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFED
                     GGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGD
                     IKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEG
                     RHSTGGMDELYK"
     misc_feature    7220..7243
                     /label=Spacer
     misc_feature    7250..7838
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7721..7732)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7870..8346
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     enhancer        8384..8615
                     /label=core insul
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#0080ff"
     repeat_region   join(8791..8806,8808..8809,8841..8853)
                     /label=5'TR
     repeat_region   8810..8840
                     /label=5'ITR
     primer_bind     complement(8896..8915)
                     /label=BII(R)
                     /note="ApEinfo_revcolor=#19ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#5381ff"
     CDS             9727..10584
                     /codon_start=1
                     /label=Amp
                     /note="ApEinfo_revcolor=#c3c2c4
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#7ab3ee"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11701..11720
                     /label=pLac(F)
                     /note="ApEinfo_revcolor=#19ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#5381ff"
     repeat_region   12239..12273
                     /label=3'ITR
     enhancer        12547..12777
                     /label=core insul
                     /label=core insul(1)
                     /note="ApEinfo_revcolor=#690f96
                     ApEinfo_label=core insul
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff00ff"
     protein_bind    complement(12789..12822)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    12823..13047
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    13048..13063
                     /label=PCR2
     CDS             complement(13067..13777)
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    complement(13805..13831)
                     /label=mycNLS
     misc_feature    complement(13847..13873)
                     /label=mycNLS
     CDS             complement(13880..13933)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(13943..14539)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    14540..14545
                     /label=Kozak
     intron          14562..15500
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     promoter        complement(15501..15712)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    complement(15713..15746)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    15747..16559
                     /label=chick cHS4 insulator(1)
     misc_feature    16560..16957
                     /label=chick cHS4
     misc_feature    16970..17971
                     /label=SRF-UCOE
ORIGIN
        1 ggcgcgccga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      361 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
      421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      481 tgcagcgatg ggggcggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag
      541 gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
      601 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
      661 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
      721 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
      781 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
      841 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
      901 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
      961 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     1021 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     1081 ggtgtgtgcg tggggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     1141 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     1201 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     1261 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     1321 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1381 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     1441 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     1501 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     1561 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     1621 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     1681 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaaggtac
     1741 ctcgcgagcc gccaccatga acaactctca gggcagagtg accttcgagg acgtgaccgt
     1801 gaacttcacc caaggcgagt ggcagagact gaaccccgag cagagaaacc tgtaccggga
     1861 cgtgatgctg gaaaactaca gcaacctggt gtccgtcggc cagggcgaga caacaaagcc
     1921 tgacgtgatc ctgcggctgg aacagggcaa agagccttgg ctggaagagg aagaggtgct
     1981 cggatctggc agagccgaga agaatggcga catcggcgga cagatctgga agcccaagga
     2041 cgtgaaagag tctctgagtg gcggctcaag tggcgggtct agtgggagtg aaacgccagg
     2101 gacttcagaa tcagcaaccc cggaatcatc cggcggatca tccggtgggt ctgtatacat
     2161 ggacaagaag tactctatcg gcctggccat cggcaccaat tctgttggat gggccgtgat
     2221 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
     2281 cagcatcaag aagaatctga tcggcgccct gctgttcgac tctggcgaaa cagccgaagc
     2341 caccagactg aagagaaccg ccagacggcg gtacaccaga agaaagaacc ggatctgcta
     2401 cctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
     2461 ggaagagtcc ttcctggtcg aggaagataa gaagcacgag cggcacccca tcttcggcaa
     2521 catcgtggat gaggtggcct accacgagaa gtaccctacc atctaccacc tccggaagaa
     2581 actggtggac agcaccgaca aggccgacct gagactgatc tatctggccc tggctcacat
     2641 gatcaagttc cggggccact ttctgatcga gggcgacctg aatcctgaca acagcgacgt
     2701 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
     2761 caacgccagc ggagtggatg ccaaggccat cctgtctgcc agactgagca agagcagacg
     2821 cctggaaaac ctgatcgctc agctgcccgg cgagaagaag aacggcctgt tcggaaacct
     2881 gattgccctg agcctgggcc tgacacctaa cttcaagagc aacttcgacc tggctgagga
     2941 cgccaaactg cagctgtcca aggacaccta cgacgacgac ctggacaatc tgctggccca
     3001 gatcggcgat cagtacgccg acttgtttct ggccgccaag aacctgtccg acgccatcct
     3061 gctgagcgac atcctgagag tgaacaccga gatcacaaag gcccctctga gcgcctctat
     3121 gatcaagaga tacgacgagc accaccagga tctgaccctg ctgaaggccc tcgttagaca
     3181 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
     3241 ctacattgat ggcggagcca gccaagagga attttacaag ttcatcaagc ccatccttga
     3301 gaagatggac ggcaccgagg aactgctggt caagctgaac agagaggacc tgctgcggaa
     3361 gcagcggacc ttcgacaatg gctctatccc tcaccagatc cacctgggag agctgcacgc
     3421 cattctgcgg agacaagagg acttttaccc attcctgaag gacaaccggg aaaagattga
     3481 gaagatcctg accttcagaa tcccctacta cgtgggacca ctggccagag gcaatagcag
     3541 attcgcctgg atgaccagaa agagcgagga aaccatcaca ccctggaact tcgaggaagt
     3601 ggtggacaag ggcgccagcg ctcagtcctt catcgagcgg atgaccaact tcgataagaa
     3661 cctgcctaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
     3721 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg cctttctgag
     3781 cggcgagcag aaaaaggcca ttgtggatct gctgttcaag accaaccgga aagtgacagt
     3841 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgacagcg tggaaatcag
     3901 cggcgtggaa gatcggttca atgccagcct gggcacatac cacgacctgc tgaaaattat
     3961 caaggacaag gacttcctgg acaacgaaga gaacgaggac atcctggaag acatcgtgct
     4021 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa catacgccca
     4081 cctgttcgac gacaaagtga tgaagcaact gaagcggcgg agatacaccg gctggggtag
     4141 actgtcccgg aagctgatca acggcatccg ggataagcag tccggcaaga ccatcctgga
     4201 ctttctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
     4261 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccaaggcg attctctgca
     4321 cgagcacatt gccaacctgg ccggatctcc cgccattaag aagggcatcc tgcagaccgt
     4381 gaaggtggtg gacgagcttg tgaaagtgat gggcagacac aagcccgaga acatcgtgat
     4441 cgaaatggcc agagagaacc agaccacaca gaagggccag aagaacagcc gcgagagaat
     4501 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
     4561 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atggacggga
     4621 tatgtacgtg gaccaagagc tggacatcaa ccggctgtcc gactacgatg tggacgctat
     4681 cgtgccccag tcttttctga aggacgactc catcgacaac aaggtcctga ccagaagcga
     4741 caagaaccgg ggcaagagcg ataacgtgcc ctccgaagag gtggtcaaga agatgaagaa
     4801 ctattggcgc cagctgctga atgccaagct gattacccag cggaagttcg acaacctgac
     4861 caaggccgaa agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
     4921 ggtggaaacc cggcagatca ccaaacacgt ggcacagatt ctggactccc ggatgaacac
     4981 taagtacgac gagaacgaca aactgatccg ggaagtgaaa gtcatcaccc tgaagtccaa
     5041 gctggtgtct gatttccgga aggatttcca gttctacaaa gtgcgcgaga tcaacaacta
     5101 ccatcacgcc cacgacgcct acctgaatgc cgttgttgga acagccctga tcaagaagta
     5161 tcccaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
     5221 gatcgccaag agcgagcaag agattggcaa ggctaccgcc aagtactttt tctactccaa
     5281 catcatgaac tttttcaaga cagagatcac cctggccaac ggcgagatcc ggaaaagacc
     5341 cctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggca gagattttgc
     5401 cacagtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaagaaaa ccgaggtgca
     5461 gacaggcggc ttcagcaaag agtctatcct gcctaagcgg aacagcgaca agctgatcgc
     5521 ccggaagaag gactgggacc caaagaagta cggcggcttc gatagcccta ccgtggccta
     5581 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactca agagcgtgaa
     5641 agaactgctc ggcatcacca tcatggaacg gtccagcttt gagaagaacc ctattgattt
     5701 ccttgaggcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tccccaagta
     5761 ctccctgttc gagctggaaa atggccggaa gcggatgctg gcttctgctg gcgaactgca
     5821 gaagggaaac gaactggccc tgcctagcaa atatgtgaac tttctgtacc tggccagcca
     5881 ctatgagaag ctgaagggca gccccgagga caatgagcaa aagcagctgt ttgtcgagca
     5941 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
     6001 cctggctgac gccaatctgg acaaagtgct gtccgcctac aacaagcacc gggacaagcc
     6061 tatcagagag caggccgaga atatcatcca cctgtttacc ctgaccaacc tgggagcccc
     6121 tgccgccttc aagtacttcg acaccaccat cgaccggaag cgctacacca gcaccaaaga
     6181 ggttctggac gccacactga tccaccagag catcaccggc ctgtacgaga caagaatcga
     6241 cctgtctcag ctcggcggcg atcctcgagg ctatccctat gacgtgcccg attatgccag
     6301 cagtggcagc ggctccccca agaaaaaacg caaggtggaa gatcctaaga aaaagcggaa
     6361 agtggttaac ggaagtggcg agggcagagg aagtctgcta acatgcggtg acgtcgagga
     6421 gaatcctggc ccacctgccg ctaaacgggt caaactcgac ggcggttctg gaggtcctgc
     6481 tgcaaagcgc gttaagttgg atgctagcat ggaatttgtg tctaaagggg aagcagtcat
     6541 taaagagttc atgcgcttca aagtacacat ggaaggaagt atgaacgggc acgaatttga
     6601 aatagaaggc gaaggggaag gtcggccata cgaagggacg caaaccgcga agttgaaggt
     6661 tacaaaggga ggaccgttgc cctttagctg ggacatcctg tctccacagt ttatgtacgg
     6721 ttcaagagct tttactaagc accccgctga catcccggac tactataaac agtcattccc
     6781 cgaaggattc aaatgggaga gggtcatgaa tttcgaggac ggaggtgctg ttacagttac
     6841 gcaggatacc tcacttgagg atggcacgct tatatataag gtgaagctcc gcggtacgaa
     6901 ctttccgccg gacggccccg ttatgcagaa gaagacgatg ggctgggaag cctctacgga
     6961 acgcctgtat cctgaggacg gtgttctgaa aggggatatt aaaatggccc ttcggttgaa
     7021 agatgggggc cgctatcttg cggactttaa gaccacctac aaagcaaaaa agcctgtcca
     7081 aatgcccggt gcatataacg tcgatagaaa acttgatata acctcccata atgaagatta
     7141 cacagtagtc gagcagtacg aacgaagcga aggtcggcac tcaactggag gtatggatga
     7201 attgtataag taggatatca tgcctaattt tgagtgtatt actttcgaaa atcaacctct
     7261 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7321 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7381 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7441 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7501 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7561 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7621 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc
     7681 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7741 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7801 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag cgctggtcga
     7861 cagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cccggaagtt
     7921 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     7981 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     8041 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     8101 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     8161 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     8221 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     8281 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     8341 gtccttgttt aaacgcgatc gcttaattaa ggccgtggag gccttttccc cgtatccccc
     8401 caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca
     8461 ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc
     8521 ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt
     8581 acatccctgg gggctttggg ggggggctgt ccctcataaa agttttgtta ctttatagaa
     8641 gaaattttga gtttttgttt tttttaataa ataaataaac ataaataaat tgtttgttga
     8701 atttattatt agtatgtaag tgtaaatata ataaaactta atatctattc aaattaataa
     8761 ataaacctcg atatacagac cgataaaaca catgcgtcaa ttttacacat gattatcttt
     8821 aacgtacgtc acaatatgat tatctttcta gggttaatct agctgcgtgt tctgcagcgt
     8881 gtcgagcatc ttcatctgct ccatcacgct gtaaaacaca tttgcaccgc gagtctgccc
     8941 gtcctccacg ggttcaaaaa cgtgaatgaa cgaggcgcgc tcactggccg tcgttttaca
     9001 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     9061 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     9121 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     9181 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     9241 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     9301 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     9361 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     9421 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     9481 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     9541 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     9601 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     9661 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     9721 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9781 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9841 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9901 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9961 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    10021 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    10081 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    10141 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    10201 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    10261 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    10321 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    10381 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    10441 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    10501 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    10561 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    10621 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    10681 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10741 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10801 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10861 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
    10921 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10981 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    11041 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    11101 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    11161 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    11221 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    11281 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    11341 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    11401 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    11461 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    11521 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    11581 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    11641 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    11701 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
    11761 aagcgcgccc gccgggtaac tcacggggta tccatgtcca tttctgcggc atccagccag
    11821 gatacccgtc ctcgctgacg taatatccca gcgccgcacc gctgtcatta atctgcacac
    11881 cggcacggca gttccggctg tcgccggtat tgttcgggtt gctgatgcgc ttcgggctga
    11941 ccatccggaa ctgtgtccgg aaaagccgcg acgaactggt atcccaggtg gcctgaacga
    12001 acagttcacc gttaaaggcg tgcatggcca caccttcccg aatcatcatg gtaaacgtgc
    12061 gttttcgctc aacgtcaatg cagcagcagt catcctcggc aaactctttc catgccgctt
    12121 caacctcgcg ggaaaaggca cgggcttctt cctccccgat gcccagatag cgccagcttg
    12181 ggcgatgact gagccggaaa aaagacccga cgatatgatc ctgatgcagc tagattaacc
    12241 ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc
    12301 taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc
    12361 gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga
    12421 gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt
    12481 atattgttat ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat
    12541 agaatcaggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct
    12601 agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc
    12661 gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct
    12721 ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc
    12781 cataccaaat aacttcgtat aatgtatgct atacgaagtt atccatagag cccaccgcat
    12841 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
    12901 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
    12961 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
    13021 cagatggctg gcaactagaa ggcacagaat tcttagcggc cgcctaatta agcttgtgcc
    13081 ccagtttgct agggaggtcg cagtatctgg ccactgccac ctcgtgctgc tcgacgtagg
    13141 tctcgttgtt ggcctccttg attctttcca gtctgtagtc cacatagtag acgccaggca
    13201 tcttgaggtt cttagcgggt ttcttggatc tatatgtggt cttggcgttt gcgatcagat
    13261 ggctcccgcc cacgagcttc agggccatgt cgtttctgcc ttccaggccg ccgtcagcgg
    13321 ggtacagcgt ctcggtgaag gcctcccagc cgagtgtttt cttctgcatc acagggccgt
    13381 tggatgtgaa gttcacccct ctgatcttga cgttgtagat gaggcagccg tcctggaggc
    13441 tggtgtcctg ggtagcggtc agcacgcccc cgtcttcgta tgtggtgact ctctcccatg
    13501 tgaagccctc agggaaggac tgcttgaaga agtcggggat gccctgggtg tggttgatga
    13561 aggtcttgct gccgtagagg aagctagtag ccaggatgtc gaaggcgaag gggagagggc
    13621 cgccctcgac caccttgatt ctcatggtct gggtgccctc gtagggcttg ccttcgccct
    13681 cggatgtgca cttgaagtga tggttgtcca cggtgccctc catgtacagc ttcatgtgca
    13741 tgttctcctt aatcagctct tcgcccttag acaccatggt ggcggtggct ccccctgacc
    13801 ctccatccaa cttaacgcgc tttgcagcag gacctccaga accgccgtcg agtttgaccc
    13861 gtttagcggc agggaccggt gggccaggat tctcctcgac gtcaccgcat gttagcagac
    13921 ttcctctgcc ctctcccgat ccggcaccgg gcttgcgggt catgcaccag gtgcgcggtc
    13981 cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt
    14041 tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc gcctccactc
    14101 cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag actccgacgg
    14161 tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct tccatctgtt
    14221 gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc tcggcgaaca
    14281 ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc cacggcggcg ccgtcgtccg
    14341 cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg
    14401 tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg tagtcggcga
    14461 acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg aggcgcaccg
    14521 tgggcttgta ctcggtcatg gtgggattta aatcacgaca cctgaaatgg aagaaaaaaa
    14581 ctttgaacca ctgtctgagg cttgagaatg aaccaagatc caaactcaaa aagggcaaat
    14641 tccaaggaga attacatcaa gtgccaagct ggcctaactt cagtctccac ccactcagtg
    14701 tggggaaact ccatcgcata aaacccctcc ccccaaccta aagacgacgt actccaaaag
    14761 cgcgagaact aatcgaggtg cctggacggc gcccggtact ccgtggagtc acatgaagcg
    14821 acggctgagg acggaaaggc ccttttcctt tgtgtgggtg actcacccgc ccgctctccc
    14881 gagcgccgcg tcctccattt tgagctccct gcagcagggc cgggaagcgg ccatctttcc
    14941 gctcacgcaa ctggtgccga ccgggccagc cttgccgccc agggcggggc gatacacggc
    15001 ggcgcgaggc caggcaccag agcaggccgg ccagcttgag actacccccg tccgattctc
    15061 ggtggccgcg ctcgcaggcc ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg
    15121 tcgccgcccg cggccccaaa aaccgaaata ccagtgtgca gatcttggcc cgcatttaca
    15181 agactatctt gccagaaaaa aagcgtcgca gcaggtcatc aaaaatttta aatggctaga
    15241 gacttatcga aagcagcgag acaggcgcga aggtgccacc agattcgcac gcggcggccc
    15301 cagcgcccag gccaggcctc aactcaagca cgaggcgaag gggctcctta agcgcaaggc
    15361 ctcgaactct cccacccact tccaacccga agctcgggat caagaatcac gtactgcagc
    15421 caggtggaag taattcaagg cacgcaaggg ccataacccg taaagaggcc aggcccgcgg
    15481 gaaccacaca cggcacttac ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc
    15541 acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag gcggagccag
    15601 tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc ggttcaattg
    15661 ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc ccataacttc
    15721 gtataatgta tgctatacga agttataata ttctcactga ctccgtcctg gagttggatg
    15781 agagataatg gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc
    15841 ttctccaaag agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc
    15901 cccggaggtg atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg
    15961 tgggtgggtt ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga
    16021 cgattgtatg aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca
    16081 gctgtatctc tccgggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac
    16141 atgcaggctc agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc
    16201 tgggttttaa cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga
    16261 acgctgtgac ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct
    16321 gtcctgcact tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat
    16381 gtgtcacccc agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg
    16441 gctttacact gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga
    16501 gggtggtgac gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc
    16561 attcaaggcc aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca
    16621 catagcaggg ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat
    16681 gattctgtca ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt
    16741 gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc
    16801 cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc
    16861 ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc
    16921 cctgggggct ttgggggggg gctgtccccg tgagctccac gtgcctaggg cacacgacca
    16981 caattccact gaaagcattt taatacggaa cttgtcactc ccggggagcc tccgctcagc
    17041 cggcagttgg ttcatttcaa tccccacgac aacccttcaa agtgcagggc agacagcagg
    17101 tggctctgcc caggcgcctg gatcacagcc cggcctgcag ccctcacctg ggcgcgggga
    17161 gaccctgagg acgctcctcc aggcggcgct ggccggggcc tgcggacacg gacgggcggg
    17221 ctgagctccg ggacccctcc ccgcgccccg caccccgcac cccgcacccc gcaccccgca
    17281 cccggcgctc acccgtccca gccccgccgc ccgcagcccc agctgcaacg cagccaccgc
    17341 cgccatcgca cccggccccg cgggcgcttc cgggacgcag gaggcatctg catccggggc
    17401 gccgctgagt cccgcccaga gccccgcccc cggctccggg ttctgcgagc ggcttccgcc
    17461 gggctgctcc gcgggcgcgt cggccatgag cgagttgccg ggcgacgtgc gggcgtttct
    17521 gcgggagcac ccgagcctgc ggctccagac ggacgcccgc aaggttcgca gcgcgggagg
    17581 ggaacggagt ggcggagaag ggcgcagttg ggatgagggg ctgaggggag ggcaggggag
    17641 aggagagggc aggggagagg ggagagggga gagcaggaga gaggggaagg caggggagag
    17701 ggcgcggcgg gatcagggga ggagagggaa gggggcgcgg caggaggggg caccagggag
    17761 cggagccctg gccctcctga cgtcctgccc gcccacgcgt ccgcaggtga ggtgcatcct
    17821 gacaggtcac gagctgccct gccgcctgcc ggagctccag gtctacaccc gcggcaaaaa
    17881 gtaccagcgg ctggtccgcg cctccccggc cttcgactat gcagagttcg agccgcacat
    17941 cgtgcccagc accaagaacc cgtaggtggt c
//
