LOCUS       Exported               18968 bp ds-DNA     circular SYN 11-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18968)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 26, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..18968
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          6143..7165
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          12351..17105
                     /label=source
     source          16974..16979
                     /label=source
     source          16980..16982
                     /label=source
     source          17037..17105
                     /label=Donor sequence
     enhancer        19..250
                     /label=core insul
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#0080ff"
     repeat_region   join(426..441,443..444,476..488)
                     /label=5'TR
     repeat_region   445..475
                     /label=5'ITR
     primer_bind     complement(531..550)
                     /label=BII(R)
                     /note="ApEinfo_revcolor=#19ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#5381ff"
     CDS             1362..2219
                     /codon_start=1
                     /label=Amp
                     /note="ApEinfo_revcolor=#c3c2c4
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#7ab3ee"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3336..3355
                     /label=pLac(F)
                     /note="ApEinfo_revcolor=#19ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#5381ff"
     repeat_region   3874..3908
                     /label=3'ITR
     enhancer        4182..4412
                     /label=core insul
                     /label=core insul(1)
                     /note="ApEinfo_revcolor=#690f96
                     ApEinfo_label=core insul
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff00ff"
     protein_bind    complement(4424..4457)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    4458..4682
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(4691..5236)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             complement(5243..5296)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5297..6088)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aph(3')-II (or nptII) aminoglycoside 
                     phosphotransferase from Tn5"
                     /label=NeoR (corrected)
                     /label=Neo-star
                     /note="NeoR/KanR;confers resistance to neomycin, kanamycin,
                     and G418 (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     variation       5543..5545
                     /label=Glu (Normal Neo)
     CDS             complement(6089..6142)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    6143..7165
                     /label=iAA61 hygro_var
                     /note="cut bb AsiSI and NheI"
     CDS             complement(6143..7165)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=Hygro
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    7166..7174
                     /label=Kozak
     intron          7193..8131
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     promoter        complement(8132..8343)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    complement(8344..8377)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    8378..9190
                     /label=chick cHS4 insulator(1)
     misc_feature    9191..9588
                     /label=chick cHS4
     misc_feature    9601..10602
                     /label=SRF-UCOE
     misc_feature    10611..10963
                     /label=CMV enhancer
     enhancer        10964..10990
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        10992..12237
                     /label=CAG
                     /note="Common feature added by Mo Mandegar"
     enhancer        10992..10993
                     /label=CAG
                     /note="Common feature added by Mo Mandegar"
     intron          12245..12338
                     /label=Mini-Intron
                     /note="Common feature added by Mo Mandegar
                     "
     regulatory      12351..12359
                     /regulatory_class="other"
                     /label=Kozak
                     /label=vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)
     misc_feature    12356..12374
                     /label=Q5 tm 67
     misc_feature    12360..17105
                     /label=from pJB69
     CDS             12360..12659
                     /codon_start=1
                     /label=ZIM3 (1-100)
                     /translation="MNNSQGRVTFEDVTVNFTQGEWQRLNPEQRNLYRDVMLENYSNLV
                     SVGQGETTKPDVILRLEQGKEPWLEEEEVLGSGRAEKNGDIGGQIWKPKDVKESL"
     CDS             12660..12755
                     /codon_start=1
                     /label=XTENsion
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             12762..16865
                     /label=dCas9
     misc_feature    16875..17105
                     /label=211013 iB22.B
     CDS             16875..16901
                     /label=HA
     CDS             16920..16940
                     /label=SV40 NLS
     misc_feature    complement(16938..16967)
                     /label=Tm 71.1˚C
     CDS             16947..16967
                     /label=SV40 NLS
     misc_feature    16957..17105
                     /label=iAA53 zim3_p2a_mapple_gb
     misc_feature    16974..16979
                     /label=from pJB69
     misc_feature    16974..16979
                     /label=iAA53 zim3_p2a_mapple_gb
     misc_feature    16974..16979
                     /label=211013 iB22.B
     misc_feature    16980..16982
                     /label=from pJB69
     misc_feature    16980..16982
                     /label=iAA53 zim3_p2a_mapple_gb
     misc_feature    16980..16982
                     /label=211013 iB22.B
     CDS             16983..17036
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_signal     17037..17105
                     /label=SeqVer (AAV F)
     misc_feature    17037..17063
                     /label=mycNLS
     misc_feature    17079..17105
                     /label=mycNLS
     misc_feature    17112..17813
                     /label=211013 iB22.B
     CDS             17112..17813
                     /codon_start=1
                     /label=mScarlet
                     /translation="MEFVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFED
                     GGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGD
                     IKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEG
                     RHSTGGMDELYK"
     misc_feature    17823..17846
                     /label=Spacer
     misc_feature    17853..18441
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(18324..18335)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    18473..18949
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
ORIGIN
        1 attaaggccg tggaggcctt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca
       61 gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc
      121 gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc
      181 tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct ttgggggggg
      241 gctgtccctc ataaaagttt tgttacttta tagaagaaat tttgagtttt tgtttttttt
      301 aataaataaa taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa
      361 atataataaa acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata
      421 aaacacatgc gtcaatttta cacatgatta tctttaacgt acgtcacaat atgattatct
      481 ttctagggtt aatctagctg cgtgttctgc agcgtgtcga gcatcttcat ctgctccatc
      541 acgctgtaaa acacatttgc accgcgagtc tgcccgtcct ccacgggttc aaaaacgtga
      601 atgaacgagg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      661 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      721 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
      781 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      841 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      901 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      961 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     1021 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1081 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     1141 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     1201 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     1261 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1321 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1381 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1441 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1501 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1561 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1621 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1681 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1741 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1801 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1861 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     1921 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1981 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2041 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2101 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2161 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2221 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2281 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2341 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2401 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2461 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2521 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2581 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2641 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2701 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2761 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2821 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2881 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2941 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3001 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3061 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3121 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3181 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     3241 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     3301 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     3361 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcccgccgg gtaactcacg
     3421 gggtatccat gtccatttct gcggcatcca gccaggatac ccgtcctcgc tgacgtaata
     3481 tcccagcgcc gcaccgctgt cattaatctg cacaccggca cggcagttcc ggctgtcgcc
     3541 ggtattgttc gggttgctga tgcgcttcgg gctgaccatc cggaactgtg tccggaaaag
     3601 ccgcgacgaa ctggtatccc aggtggcctg aacgaacagt tcaccgttaa aggcgtgcat
     3661 ggccacacct tcccgaatca tcatggtaaa cgtgcgtttt cgctcaacgt caatgcagca
     3721 gcagtcatcc tcggcaaact ctttccatgc cgcttcaacc tcgcgggaaa aggcacgggc
     3781 ttcttcctcc ccgatgccca gatagcgcca gcttgggcga tgactgagcc ggaaaaaaga
     3841 cccgacgata tgatcctgat gcagctagat taaccctaga aagatagtct gcgtaaaatt
     3901 gacgcatgca ttcttgaaat attgctctct ctttctaaat agcgcgaatc cgtcgctgtg
     3961 catttaggac atctcagtcg ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta
     4021 agtgtcactg attttgaact ataacgaccg cgtgagtcaa aatgacgcat gattatcttt
     4081 tacgtgactt ttaagattta actcatacga taattatatt gttatttcat gttctactta
     4141 cgtgataact tattatatat atattttctt gttatagaat cagggacagc ccccccccaa
     4201 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     4261 gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc
     4321 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
     4381 gcctgcagac acctgggggg atacggggaa aaagccatac caaataactt cgtataatgt
     4441 atgctatacg aagttatcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
     4501 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
     4561 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
     4621 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
     4681 agaattccta acccagcccg ggttttccaa ggcgatgccc ctcatgagcc aggagccatt
     4741 ccttcacagc aagtcctcct tcgtatcctc cgacgtccaa gtcgccttgg accacccgat
     4801 ggcacggaat gagtattggg actgggtttc cagacaacgc cgtttttact gccgccgttg
     4861 cggcaggatt cccagccaag gccgccagat gactatagct gattacctcg ccaaatttca
     4921 ctaccttcaa gagcttccac aatacctgtc ttgtgaatga ctcttgctgg aagacaggat
     4981 gatggagcgc cggtactggg aactcttcta tggcctcggg ttggtggaag taggcgttca
     5041 accaggctgt cgcttgcatg agaggttctg gaccgccaag taccgcggcg ggcgcaggca
     5101 cctctaccgc gtccgcggcg ctggtcccct tcccaaggaa aataatccgg tgaagcccct
     5161 gttcacatcc tgacagttcc agcttaccaa gaggactgtc caaggtagtg cgtttcatct
     5221 cgcagtcctt atccatgaat tcgggacctg gattctcttc gacatccccg caagtcagga
     5281 gagagccccg accctcaaaa aactcgtcga ggagcctgta gaatgcaatc ctttggctgt
     5341 caggtgcagc aatcccgtac aatacaagaa atcggtcggc ccattcccca cccagctcct
     5401 cggcaatatc cctggttgca agagcgatgt cctggtaccg gtcagcgaca ccgagtctcc
     5461 cgcagtctat gaacccggaa aatctaccat tctcgaccat aatatttgga aggcatgcat
     5521 cgccatgcgt aacaaccaag tcctcaccat ctggcatccg tgctttaagt cttgcaaaca
     5581 actccgcggg ggcgagaccc tgatgttcct catccaagtc atcctggtct acaagaccag
     5641 cctccattcg ggttctagct ctctcgatcc gatgtttcgc ttggtggtca aacgggcaag
     5701 tcgcagggtc aagcgtatgg aggcggcgca tagcatcagc cataatagac accttttctg
     5761 caggggcaag gtggcttgag agcaagtctt gtccaggtac ctcacccaac aaaagccaat
     5821 ctctcccggc ctctgtcaca acgtcgagaa ccgcggcgca gggtaccccc gtggtcgcaa
     5881 gccaagagag gcgagccgct tcgtcttgaa gctcattgag ggcaccgctc aaatcggttt
     5941 taacaaaaag aactgggcgg ccttgagcgg agagtcgaaa aacggcggca tcactacacc
     6001 ctattgtctg ctgagcccaa tcgtaaccga acagtctttc aacccatgca gccggtgatc
     6061 cagcatggag cccgtcttgt tcgatcatcg gtcccggatt ctcttccaca tctccacagg
     6121 taaggagaga acctcgtccc tcctctttag ctcgcgggcg agtagaaggc cggcgattac
     6181 ctgaatccgc caatacctcg acgcagccgt cggtccatac agccgcactc cttcgagcta
     6241 tctgtgttct gccgacggta ccggctccac tccttactat tgcatcacac ctaccttgag
     6301 cccacgccgc atcatcgaag ttcccatcta cgagtgactg atagagctga tccagtccaa
     6361 tcctcaacat gtacgcgcgc aatctcggac tacctgccaa ttcaggatgg cgtcgctcaa
     6421 aatacctcgt ttgttgctcc atgcatgcga gccagggccg ccagaaaaat atatttgcta
     6481 cctcatattg tgagtcgccg aacatcgcct ctgaccagtc tataacagcg gttatccgcc
     6541 cattatccgt caaaacgtta tttgaaccga agtcagcgtg aaccaagtgg cgtacctcag
     6601 gacagtcttc tgcccagagc atcagttcat caagcgcttg cgcgactgag gcagaaaccg
     6661 tatcatccat taccgtttgc caatggtaga cgtgcggatc tgcaatggcg cagatgaagt
     6721 ctctccaagt cgtgtattgg cctatgcctt gcggaccaaa ggggccgaat ccggacgtct
     6781 gagagagatc agccgcggct atggcatcca tagcttcagc gaccggttgg agaacggctg
     6841 gaagctcggt ttccggcaga tcctgaagtg taaccccctg agcgcggcga ctgatgcagt
     6901 aagtcaggct ctcagaaaat tcaccaatat ccaatacttc gggaatgggg agtgcagcgg
     6961 aagcgaagtg tctatataca tacctgtcct tgtagaatcc gtctgcgcag gagttcaccc
     7021 tgaggacata gcccctcccc cctacatcaa aggagaaggc tcgggactcc tctccttctg
     7081 aaagctgcat cagatcgcta actgaatcaa atttttctat caagaacttt tcaacagatg
     7141 ttgcagtcaa ctctggcttc ttcatggtgg cggcggccgc aaatcacgac acctgaaatg
     7201 gaagaaaaaa actttgaacc actgtctgag gcttgagaat gaaccaagat ccaaactcaa
     7261 aaagggcaaa ttccaaggag aattacatca agtgccaagc tggcctaact tcagtctcca
     7321 cccactcagt gtggggaaac tccatcgcat aaaacccctc cccccaacct aaagacgacg
     7381 tactccaaaa gcgcgagaac taatcgaggt gcctggacgg cgcccggtac tccgtggagt
     7441 cacatgaagc gacggctgag gacggaaagg cccttttcct ttgtgtgggt gactcacccg
     7501 cccgctctcc cgagcgccgc gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg
     7561 gccatctttc cgctcacgca actggtgccg accgggccag ccttgccgcc cagggcgggg
     7621 cgatacacgg cggcgcgagg ccaggcacca gagcaggccg gccagcttga gactaccccc
     7681 gtccgattct cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg
     7741 cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc agatcttggc
     7801 ccgcatttac aagactatct tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt
     7861 aaatggctag agacttatcg aaagcagcga gacaggcgcg aaggtgccac cagattcgca
     7921 cgcggcggcc ccagcgccca ggccaggcct caactcaagc acgaggcgaa ggggctcctt
     7981 aagcgcaagg cctcgaactc tcccacccac ttccaacccg aagctcggga tcaagaatca
     8041 cgtactgcag ccaggtggaa gtaattcaag gcacgcaagg gccataaccc gtaaagaggc
     8101 caggcccgcg ggaaccacac acggcactta cctgtgttct ggcggcaaac ccgttgcgaa
     8161 aaagaacgtt cacggcgact actgcactta tatacggttc tcccccaccc tcgggaaaaa
     8221 ggcggagcca gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac
     8281 cggttcaatt gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg
     8341 cccataactt cgtataatgt atgctatacg aagttataat attctcactg actccgtcct
     8401 ggagttggat gagagataat ggccttacgt tgtgccaggg gagggtcggg ctggatttag
     8461 caagatttac cttctccaaa gagcggtgct gcagtggcac agctgcccac ggaggtgggg
     8521 gggtcaccgt ccccggaggt gatgaagaac tgtggggatg tggcactgag ggacatggcc
     8581 agtgggcacg gtgggtgggt tggggttggt cttggggatc ttggagggct tttccagcct
     8641 tcatgatttg acgattgtat gaacatctac atggcaattc tccagctgcc tgtcccagtc
     8701 ctactgaccc agctgtatct ctccgggcaa gctcttccac cccttctgct tgcatccaga
     8761 caccatcaaa catgcaggct cagacacagg gaccagcagt gtctgtggcc tttttgtgct
     8821 cctctccatg ctgggtttta acttgctctt tgtccttcta tcctatcttc ttatccttaa
     8881 ggctgttctg aacgctgtga cttggagagt gtcccagagc cctcaacacc tgcatgtccc
     8941 acgtccatgc tgtcctgcac ttccttatcc ccaagatctg cctctccgtg atgcactgaa
     9001 ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc aaactccccc ttgataggac
     9061 aagggggaat ggctttacac tgagacaggg gaggtttggg ttggatatga ggaggcagtt
     9121 tttcccccag agggtggtga cgcactgaac aggttgccca aggaggctgt ggatgcccca
     9181 tccctgcagg cattcaaggc caggctggat gtggctctgg gcagcctggg ctgctggttg
     9241 atgaccctgc acatagcagg gggttggatc tggatgagca ctgtgctcct ttgcaaccca
     9301 ggccgttcta tgattctgtc attctaaatc tctctttcag cctaaagctt tttccccgta
     9361 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     9421 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     9481 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
     9541 gtaattacat ccctgggggc tttggggggg ggctgtcccc gtgagctcca cgtgcctagg
     9601 gcacacgacc acaattccac tgaaagcatt ttaatacgga acttgtcact cccggggagc
     9661 ctccgctcag ccggcagttg gttcatttca atccccacga caacccttca aagtgcaggg
     9721 cagacagcag gtggctctgc ccaggcgcct ggatcacagc ccggcctgca gccctcacct
     9781 gggcgcgggg agaccctgag gacgctcctc caggcggcgc tggccggggc ctgcggacac
     9841 ggacgggcgg gctgagctcc gggacccctc cccgcgcccc gcaccccgca ccccgcaccc
     9901 cgcaccccgc acccggcgct cacccgtccc agccccgccg cccgcagccc cagctgcaac
     9961 gcagccaccg ccgccatcgc acccggcccc gcgggcgctt ccgggacgca ggaggcatct
    10021 gcatccgggg cgccgctgag tcccgcccag agccccgccc ccggctccgg gttctgcgag
    10081 cggcttccgc cgggctgctc cgcgggcgcg tcggccatga gcgagttgcc gggcgacgtg
    10141 cgggcgtttc tgcgggagca cccgagcctg cggctccaga cggacgcccg caaggttcgc
    10201 agcgcgggag gggaacggag tggcggagaa gggcgcagtt gggatgaggg gctgagggga
    10261 gggcagggga gaggagaggg caggggagag gggagagggg agagcaggag agaggggaag
    10321 gcaggggaga gggcgcggcg ggatcagggg aggagaggga agggggcgcg gcaggagggg
    10381 gcaccaggga gcggagccct ggccctcctg acgtcctgcc cgcccacgcg tccgcaggtg
    10441 aggtgcatcc tgacaggtca cgagctgccc tgccgcctgc cggagctcca ggtctacacc
    10501 cgcggcaaaa agtaccagcg gctggtccgc gcctccccgg ccttcgacta tgcagagttc
    10561 gagccgcaca tcgtgcccag caccaagaac ccgtaggtgg tcggcgcgcc gacattgatt
    10621 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
    10681 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
    10741 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
    10801 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
    10861 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
    10921 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
    10981 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
    11041 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
    11101 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
    11161 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
    11221 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
    11281 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
    11341 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
    11401 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
    11461 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
    11521 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
    11581 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
    11641 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
    11701 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
    11761 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
    11821 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
    11881 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
    11941 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
    12001 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
    12061 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
    12121 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
    12181 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
    12241 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
    12301 gctggttatt gtgctgtctc atcattttgg caaagaatgg tacctcgcga gccgccacca
    12361 tgaacaactc tcagggcaga gtgaccttcg aggacgtgac cgtgaacttc acccaaggcg
    12421 agtggcagag actgaacccc gagcagagaa acctgtaccg ggacgtgatg ctggaaaact
    12481 acagcaacct ggtgtccgtc ggccagggcg agacaacaaa gcctgacgtg atcctgcggc
    12541 tggaacaggg caaagagcct tggctggaag aggaagaggt gctcggatct ggcagagccg
    12601 agaagaatgg cgacatcggc ggacagatct ggaagcccaa ggacgtgaaa gagtctctga
    12661 gtggcggctc aagtggcggg tctagtggga gtgaaacgcc agggacttca gaatcagcaa
    12721 ccccggaatc atccggcgga tcatccggtg ggtctgtata catggacaag aagtactcta
    12781 tcggcctggc catcggcacc aattctgttg gatgggccgt gatcaccgac gagtacaagg
    12841 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaatc
    12901 tgatcggcgc cctgctgttc gactctggcg aaacagccga agccaccaga ctgaagagaa
    12961 ccgccagacg gcggtacacc agaagaaaga accggatctg ctacctgcaa gagatcttca
    13021 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg
    13081 tcgaggaaga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gatgaggtgg
    13141 cctaccacga gaagtaccct accatctacc acctccggaa gaaactggtg gacagcaccg
    13201 acaaggccga cctgagactg atctatctgg ccctggctca catgatcaag ttccggggcc
    13261 actttctgat cgagggcgac ctgaatcctg acaacagcga cgtggacaag ctgttcatcc
    13321 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggagtgg
    13381 atgccaaggc catcctgtct gccagactga gcaagagcag acgcctggaa aacctgatcg
    13441 ctcagctgcc cggcgagaag aagaacggcc tgttcggaaa cctgattgcc ctgagcctgg
    13501 gcctgacacc taacttcaag agcaacttcg acctggctga ggacgccaaa ctgcagctgt
    13561 ccaaggacac ctacgacgac gacctggaca atctgctggc ccagatcggc gatcagtacg
    13621 ccgacttgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga
    13681 gagtgaacac cgagatcaca aaggcccctc tgagcgcctc tatgatcaag agatacgacg
    13741 agcaccacca ggatctgacc ctgctgaagg ccctcgttag acagcagctg cctgagaagt
    13801 acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt gatggcggag
    13861 ccagccaaga ggaattttac aagttcatca agcccatcct tgagaagatg gacggcaccg
    13921 aggaactgct ggtcaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca
    13981 atggctctat ccctcaccag atccacctgg gagagctgca cgccattctg cggagacaag
    14041 aggactttta cccattcctg aaggacaacc gggaaaagat tgagaagatc ctgaccttca
    14101 gaatccccta ctacgtggga ccactggcca gaggcaatag cagattcgcc tggatgacca
    14161 gaaagagcga ggaaaccatc acaccctgga acttcgagga agtggtggac aagggcgcca
    14221 gcgctcagtc cttcatcgag cggatgacca acttcgataa gaacctgcct aacgagaagg
    14281 tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag
    14341 tgaaatacgt gaccgaggga atgagaaagc ccgcctttct gagcggcgag cagaaaaagg
    14401 ccattgtgga tctgctgttc aagaccaacc ggaaagtgac agtgaagcag ctgaaagagg
    14461 actacttcaa gaaaatcgag tgcttcgaca gcgtggaaat cagcggcgtg gaagatcggt
    14521 tcaatgccag cctgggcaca taccacgacc tgctgaaaat tatcaaggac aaggacttcc
    14581 tggacaacga agagaacgag gacatcctgg aagacatcgt gctgaccctg acactgtttg
    14641 aggacagaga gatgatcgag gaacggctga aaacatacgc ccacctgttc gacgacaaag
    14701 tgatgaagca actgaagcgg cggagataca ccggctgggg tagactgtcc cggaagctga
    14761 tcaacggcat ccgggataag cagtccggca agaccatcct ggactttctg aagtccgacg
    14821 gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg
    14881 acatccagaa agcccaggtg tccggccaag gcgattctct gcacgagcac attgccaacc
    14941 tggccggatc tcccgccatt aagaagggca tcctgcagac cgtgaaggtg gtggacgagc
    15001 ttgtgaaagt gatgggcaga cacaagcccg agaacatcgt gatcgaaatg gccagagaga
    15061 accagaccac acagaagggc cagaagaaca gccgcgagag aatgaagcgg atcgaagagg
    15121 gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc
    15181 agaacgagaa gctgtacctg tactacctgc agaatggacg ggatatgtac gtggaccaag
    15241 agctggacat caaccggctg tccgactacg atgtggacgc tatcgtgccc cagtcttttc
    15301 tgaaggacga ctccatcgac aacaaggtcc tgaccagaag cgacaagaac cggggcaaga
    15361 gcgataacgt gccctccgaa gaggtggtca agaagatgaa gaactattgg cgccagctgc
    15421 tgaatgccaa gctgattacc cagcggaagt tcgacaacct gaccaaggcc gaaagaggcg
    15481 gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga
    15541 tcaccaaaca cgtggcacag attctggact cccggatgaa cactaagtac gacgagaacg
    15601 acaaactgat ccgggaagtg aaagtcatca ccctgaagtc caagctggtg tctgatttcc
    15661 ggaaggattt ccagttctac aaagtgcgcg agatcaacaa ctaccatcac gcccacgacg
    15721 cctacctgaa tgccgttgtt ggaacagccc tgatcaagaa gtatcccaag ctggaaagcg
    15781 agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc
    15841 aagagattgg caaggctacc gccaagtact ttttctactc caacatcatg aactttttca
    15901 agacagagat caccctggcc aacggcgaga tccggaaaag acccctgatc gagacaaacg
    15961 gcgaaaccgg ggagatcgtg tgggataagg gcagagattt tgccacagtg cggaaagtgc
    16021 tgagcatgcc ccaagtgaat atcgtgaaga aaaccgaggt gcagacaggc ggcttcagca
    16081 aagagtctat cctgcctaag cggaacagcg acaagctgat cgcccggaag aaggactggg
    16141 acccaaagaa gtacggcggc ttcgatagcc ctaccgtggc ctattctgtg ctggtggtgg
    16201 ccaaagtgga aaagggcaag tccaagaaac tcaagagcgt gaaagaactg ctcggcatca
    16261 ccatcatgga acggtccagc tttgagaaga accctattga tttccttgag gccaagggct
    16321 acaaagaagt gaaaaaggac ctgatcatca agctccccaa gtactccctg ttcgagctgg
    16381 aaaatggccg gaagcggatg ctggcttctg ctggcgaact gcagaaggga aacgaactgg
    16441 ccctgcctag caaatatgtg aactttctgt acctggccag ccactatgag aagctgaagg
    16501 gcagccccga ggacaatgag caaaagcagc tgtttgtcga gcagcacaag cactacctgg
    16561 acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggct gacgccaatc
    16621 tggacaaagt gctgtccgcc tacaacaagc accgggacaa gcctatcaga gagcaggccg
    16681 agaatatcat ccacctgttt accctgacca acctgggagc ccctgccgcc ttcaagtact
    16741 tcgacaccac catcgaccgg aagcgctaca ccagcaccaa agaggttctg gacgccacac
    16801 tgatccacca gagcatcacc ggcctgtacg agacaagaat cgacctgtct cagctcggcg
    16861 gcgatcctcg aggctatccc tatgacgtgc ccgattatgc cagcagtggc agcggctccc
    16921 ccaagaaaaa acgcaaggtg gaagatccta agaaaaagcg gaaagtggtt aacggaagtg
    16981 gcgagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccacctg
    17041 ccgctaaacg ggtcaaactc gacggcggtt ctggaggtcc tgctgcaaag cgcgttaagt
    17101 tggatgctag catggaattt gtgtctaaag gggaagcagt cattaaagag ttcatgcgct
    17161 tcaaagtaca catggaagga agtatgaacg ggcacgaatt tgaaatagaa ggcgaagggg
    17221 aaggtcggcc atacgaaggg acgcaaaccg cgaagttgaa ggttacaaag ggaggaccgt
    17281 tgccctttag ctgggacatc ctgtctccac agtttatgta cggttcaaga gcttttacta
    17341 agcaccccgc tgacatcccg gactactata aacagtcatt ccccgaagga ttcaaatggg
    17401 agagggtcat gaatttcgag gacggaggtg ctgttacagt tacgcaggat acctcacttg
    17461 aggatggcac gcttatatat aaggtgaagc tccgcggtac gaactttccg ccggacggcc
    17521 ccgttatgca gaagaagacg atgggctggg aagcctctac ggaacgcctg tatcctgagg
    17581 acggtgttct gaaaggggat attaaaatgg cccttcggtt gaaagatggg ggccgctatc
    17641 ttgcggactt taagaccacc tacaaagcaa aaaagcctgt ccaaatgccc ggtgcatata
    17701 acgtcgatag aaaacttgat ataacctccc ataatgaaga ttacacagta gtcgagcagt
    17761 acgaacgaag cgaaggtcgg cactcaactg gaggtatgga tgaattgtat aagtaggata
    17821 tcatgcctaa ttttgagtgt attactttcg aaaatcaacc tctggattac aaaatttgtg
    17881 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
    17941 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
    18001 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
    18061 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
    18121 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
    18181 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
    18241 cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg
    18301 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
    18361 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
    18421 ccctttgggc cgcctccccg catcgatacc gagcgctggt cgacagatct acgggtggca
    18481 tccctgtgac ccctccccag tgcctctcct ggccccggaa gttgccactc cagtgcccac
    18541 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
    18601 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
    18661 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
    18721 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
    18781 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
    18841 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
    18901 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttg tttaaacgcg
    18961 atcgctta
//
