LOCUS       RV-CAG-DIO-mScarlet-T2A 7862 bp ds-DNA     circular     18-MAY-2022
DEFINITION  .
KEYWORDS    "Plasmid name:RV-CAG-DIO-mScarlet-T2A" "Resistance:Amp" "Box:3"
            "old lab code" "Addgene cat no" "received from"
            "insert:mScarlet-T2A" "promoter:CAG" "backbone:PIntD069"
            "Author:Lynette" "Sequences" "Glycerol stock"
COMMENT     Imported using the Genbank importer. File name:
            RV-Syn-DIO-GFP-klenow.ape ApEinfo:methylated:1 2nd of March 2022
            Retransform clone because stock is running low. Sequencing result:
FEATURES             Location/Qualifiers
     misc_feature    51..644
                     /label="3'-LTR"
     rep_origin      complement(998..1680)
                     /label="ColE1 origin"
     CDS             complement(1785..2444)
                     /label="AmpR"
     misc_recomb     2859..3553
                     /label="5-prime-LTR"
     enhancer        4446..4825
                     /label="CMV enhancer"
     promoter        4827..5103
                     /label="chicken beta-actin promoter"
     intron          5104..6120
                     /label="chimeric intron"
     primer_bind     6044..6066
                     /label="pCAGGS-5"
     primer_bind     6128..6147
                     /label="pCAG-F"
     primer          6128..6147
                     /label="seq_UCOE_rev"
                     /note="sequence: gcaacgtgctggttattgtg"
     misc_feature    complement(6191..6223)
                     /label="Lox2772"
     primer          6224..6247
                     /label="LoxP_pIntD185_fwd"
                     /note="sequence: GCAGAATGGTAGCTGGATTGTAGC"
     misc_feature    6274..6306
                     /label="LoxP site"
     misc_feature    complement(6274..6306)
                     /label="Lox"
     misc_feature    complement(6322..6375)
                     /label="T2A"
     CDS             complement(6322..6375)
                     /label="Translation 6375-6322"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6369..7131)
                     /label="Translation 7131-6369"
                     /translation="MLCCMRRTKQVEKNDEDQKIMVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHSTGGMDELYKEG"
     CDS             complement(6376..7071)
                     /label="mScarlet-I"
     primer          complement(6420..6439)
                     /label="mScarlet-I_pIntD185_rev"
                     /note="sequence: GGTGGAACAGTACGAACGCT"
     primer          complement(7053..7142)
                     /label="ascI-palm_FP-fwd"
                     /note="sequence: atGGCGCGCCaccatgctgtgctgtatgagaagaaccaaacaggttgaaaagaatgatgaggaccaaaagatcatggtgagcaagggcgagg"
     misc_feature    complement(7072..7134)
                     /label="palmitation"
     misc_recomb     7146..7178
                     /label="Lox2772"
     misc_recomb     complement(7230..7263)
                     /label="LoxP"
     misc_signal     7275..7850
                     /label="WPRE"
ORIGIN
        1 TGGAATTCGA GAAAATAAAA GATTTTATTT AGTCTCCAGA AAAAGGGGGG AATGAAAGAC
       61 CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC ATGGAAAAAT
      121 ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTCAGGAA CAGATGGAAC AGCTGAATAT
      181 GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT
      241 GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
      301 GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC AGCAGTTTCT AGAGAACCAT
      361 CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC
      421 AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC
      481 CCACAACCCC TCACTCGGGG CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG
      541 TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG
      601 TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATTTCCGAC TTGTGGTCTC
      661 GCTGCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTCACATG
      721 CAGCATGTAT CAAAATTAAT TTGGTTTTTT TTCTTAAGTA TTTACATTAA ATGGCCATAG
      781 TTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG CGCTCTTCCG
      841 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
      901 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
      961 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
     1021 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
     1081 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
     1141 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
     1201 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
     1261 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
     1321 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
     1381 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
     1441 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
     1501 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
     1561 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
     1621 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
     1681 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTGCGGCCGC
     1741 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     1801 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     1861 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     1921 CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     1981 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     2041 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     2101 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     2161 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     2221 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     2281 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     2341 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAACA
     2401 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     2461 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     2521 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     2581 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     2641 ATACTCTTCC TTTTTCAATG ATCCGGCCAT TAGCCATATT ATTCATTGGT TATATAGCAT
     2701 AAATCAATAT TGGCTATTGG CCATTGCATA CGTTGTATCC ATATCATAAT ATGTACATTT
     2761 ATATTGGCTC ATGTCCAACA TTACCGCCAT GTTGACATTG ATTATTGACT AGTTATTAAT
     2821 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC
     2881 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA
     2941 TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT
     3001 ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC
     3061 CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT
     3121 GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC
     3181 GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC
     3241 TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA
     3301 AATGTCGTAA CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCATGTA CGGTGGGAGG
     3361 TCTATATAAG CAGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGGGC GCCAGTCCTC
     3421 CGATTGACTG AGTCGCCCGG GTACCCGTAT TCCCAATAAA GCCTCTTGCT GTTTGCATCC
     3481 GAATCGTGGT CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCACGACG
     3541 GGGGTCTTTC ATTTGGGGGC TCGTCCGGGA TTTGGAGACC CCTGCCCAGG GACCACCGAC
     3601 CCACCACCGG GAGGTAAGCT GGCCAGCAAC TTATCTGTGT CTGTCCGATT GTCTAGTGTC
     3661 TATGTTTGAT GTTATGCGCC TGCGTCTGTA CTAGTTAGCT AACTAGCTCT GTATCTGGCG
     3721 GACCCGTGGT GGAACTGACG AGTTCTGAAC ACCCGGCCGC AACCCTGGGA GACGTCCCAG
     3781 GGACTTTGGG GGCCGTTTTT GTGGCCCGAC CTGAGGAAGG GAGTCGATGT GGAATCCGAC
     3841 CCCGTCAGGA TATGTGGTTC TGGTAGGAGA CGAGAACCTA AAACAGTTCC CGCCTCCGTC
     3901 TGAATTTTTG CTTTCGGTTT GGAACCGAAG CCGCGCGTCT TGTCTGCTGC AGCGCTGCAG
     3961 CATCGTTCTG TGTTGTCTCT GTCTGACTGT GTTTCTGTAT TTGTCTGAAA ATTAGGGCCA
     4021 GACTGTTACC ACTCCCTTAA GTTTGACCTT AGGTCACTGG AAAGATGTCG AGCGGATCGC
     4081 TCACAACCAG TCGGTAGATG TCAAGAAGAG ACGTTGGGTT ACCTTCTGCT CTGCAGAATG
     4141 GCCAACCTTT AACGTCGGAT GGCCGCGAGA CGGCACCTTT AACCGAGACC TCATCACCCA
     4201 GGTTAAGATC AAGGTCTTTT CACCTGGCCC GCATGGACAC CCAGACCAGG TCCCCTACAT
     4261 CGTGACCTGG GAAGCCTTGG CTTTTGACCC CCCTCCCTGG GTCAAGCCCT TTGTACACCC
     4321 TAAGCCTCCG CCTCCTCTTC CTCCATCCGC CCCGTCTCTC CCCCTTGAAC CTCCTCGTTC
     4381 GACCCCGCCT CGATCCTCCC TTTATCCAGC CCTCACTCCT TCTCTAGGCG CCGGAATTCG
     4441 ATgtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     4501 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     4561 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     4621 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4681 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4741 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     4801 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     4861 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     4921 agcgatgggg gcgggggggg ggggggncgc gcgccaggcg gggcggggcg gggcgagggg
     4981 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     5041 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     5101 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     5161 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     5221 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     5281 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     5341 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     5401 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     5461 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     5521 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     5581 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     5641 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     5701 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     5761 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     5821 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     5881 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     5941 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     6001 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     6061 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     6121 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgctagc
     6181 GGATCCGGAA TAACTTCGTA TAGGATACTT TATACGAAGT TATGCAGAAT GGTAGCTGGA
     6241 TTGTAGCTGC TATTAGCAAT ATGAAACCTC TTAATAACTT CGTATAGCAT ACATTATACG
     6301 AAGTTATTAA TTAAtCTCGA GAGGGCCGGG ATTCTCTTCC ACGTCTCCGC ATGTCAGAAG
     6361 ACTTCCTCTG CCCTCCTTGT ACAGCTCGTC CATGCCGCCG GTGGAGTGGC GGCCCTCGGA
     6421 GCGTTCGTAC TGTTCCACCA CGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAACTTGCG
     6481 GTCGACGTTG TAGGCGCCGG GCATCTGCAC GGGCTTCTTG GCCTTGTAGG TGGTCTTGAA
     6541 GTCCGCCAGG TAGCGGCCGC CGTCCTTCAG GCGCAGGGCC ATCTTAATGT CGCCCTTCAG
     6601 CACGCCGTCC TCGGGGTACA ACCGCTCGGT GGACGCTTCC CAGCCCATTG TCTTCTTCTG
     6661 CATTACGGGG CCGTCAGGAG GGAAGTTGGT GCCGCGGAGC TTCACCTTGT AGATCAGGGT
     6721 GCCGTCCTCC AGGGAGGTGT CCTGGGTCAC GGTCACGGCG CCGCCGTCCT CGAAGTTCAT
     6781 CACGCGCTCC CACTTGAAGC CCTCGGGGAA GGACTGCTTA TAGTAGTCGG GGATGTCGGC
     6841 GGGGTGCTTG ATGAAGGCCC TGGAGCCGTA CATGAACTGA GGGGACAGGA TGTCCCAGGA
     6901 GAAGGGCAGG GGGCCACCCT TGGTCACCTT CAGCTTGGCG GTCTGGGTGC CCTCGTAGGG
     6961 GCGGCCCTCG CCCTCGCCCT CGATCTCGAA CTCGTGGCCG TTCATGGAGC CCTCCATGTG
     7021 CACCTTGAAC CGCATGAACT CCTTGATCAC TGCCTCGCCC TTGCTCACCA TGATCTTTTG
     7081 GTCCTCATCA TTCTTTTCAA CCTGTTTGGT TCTTCTCATA CAGCACAGCA TGGTGGCGCG
     7141 CCGCGATAAC TTCGTATAAA GTATCCTATA CGAAGTTATT TGCCTTAACC CAGAAATTAT
     7201 CACTGTTATT CTTTAGAATG GTGCAAAGAA TAACTTCGTA TAATGTATGC TATACGAAGT
     7261 TATGTTTAAA CCAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT
     7321 AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT
     7381 ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT
     7441 TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC
     7501 GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT
     7561 TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA
     7621 GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT
     7681 CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC
     7741 CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT
     7801 CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG
     7861 CC
//