LOCUS       Exported                9992 bp ds-DNA     circular SYN 08-JUN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pnCas9-PBE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 26, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9992
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..1999
                     /label=ubi promoter
                     /note="color: #ff6600; direction: RIGHT"
     CDS             2021..2041
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             2042..2512
                     /codon_start=1
                     /label=mini-Sdd7
                     /note="color: #339966"
                     /translation="EGGGPGAVPEGGDGPPAVPAEEVERLRGELPPPVVPGTGQKTHGR
                     WIGPDGRVRAIVSGRDEDAALVHAQLAAKGIPDEPTRNSDVEQKLAAHMVANGIRHVTL
                     VINHRPCRGFDDSCDTLVPIILPEGCTLTVHGQTDKGMRVRVRYTGGARPWWS"
     CDS             2513..2560
                     /codon_start=1
                     /label=XTEN
                     /note="color: #00ccff"
                     /translation="KSGSETPGTSESATPE"
     CDS             2567..6667
                     /codon_start=1
                     /label=Cas9(D10A)
                     /note="This feature has 3 segments:
                        1: 2567 .. 2590 / #993366
                        2: 2591 .. 2593 / #cc99ff
                        3: 2594 .. 6667 / #993366"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6668..6715
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6668..6715
                     /codon_start=1
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6737..6997
                     /codon_start=1
                     /label=UGI
                     /note="color: #ffcc00"
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKMLSGGS"
     CDS             6998..7018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    7048..7771
                     /label=CaMV term
                     /note="color: #ffff00; direction: RIGHT"
     polyA_signal    7092..7268
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7822..7838)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    7846..7862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(7870..7900)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 7870 .. 7876 / #ffffff / -10
                        2: 7877 .. 7894 / #ffffff
                        3: 7895 .. 7900 / #ffffff / -35"
     protein_bind    7915..7936
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(8224..8812)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(8983..9843)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8983 .. 9774 / #ccffcc
                        2: 9775 .. 9843 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9844..9948)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttgtcga cggatccatg ccaaagaaga agaggaaggt
     2041 tgagggagga gggcctgggg cggttcctga aggaggcgac ggcccgccag ccgtgccggc
     2101 ggaggaggtg gagaggttga gaggagaact tcctccacct gttgtcccgg gcactggtca
     2161 aaaaacgcat gggcgctgga ttggtccaga tggtcgcgtc cgcgccattg tttcaggaag
     2221 ggatgaagat gctgcactgg tgcatgcaca gctcgccgcc aaggggatac cagacgagcc
     2281 aacaagaaat tctgatgttg agcagaagct ggccgcgcac atggtggcaa atggcatccg
     2341 ccacgtcacg ctcgtcatca accatagacc ctgccgcggc ttcgacgaca gctgtgatac
     2401 acttgtgccc ataattctgc cggagggctg caccctcacg gtgcacgggc aaactgacaa
     2461 gggcatgagg gtgcgggtaa ggtacaccgg cggcgcgcgg ccgtggtggt ccaagtccgg
     2521 cagcgagacg ccaggcacct ccgagagcgc tacgcctgaa cttaaggaca agaagtactc
     2581 gatcggcctc gccatcggga cgaactcagt tggctgggcc gtgatcaccg acgagtacaa
     2641 ggtgccctct aagaagttca aggtcctggg gaacaccgac cgccattcca tcaagaagaa
     2701 cctcatcggc gctctcctgt tcgacagcgg ggagaccgct gaggctacga ggctcaagag
     2761 aaccgctagg cgccggtaca cgagaaggaa gaacaggatc tgctacctcc aagagatttt
     2821 ctccaacgag atggccaagg ttgacgattc attcttccac cgcctggagg agtctttcct
     2881 cgtggaggag gataagaagc acgagcggca tcccatcttc ggcaacatcg tggacgaggt
     2941 tgcctaccac gagaagtacc ctacgatcta ccatctgcgg aagaagctcg tggactccac
     3001 cgataaggcg gacctcagac tgatctacct cgctctggcc cacatgatca agttccgcgg
     3061 ccatttcctg atcgaggggg atctcaaccc agacaacagc gatgttgaca agctgttcat
     3121 ccaactcgtg cagacctaca accaactctt cgaggagaac ccgatcaacg cctctggcgt
     3181 ggacgcgaag gctatcctgt ccgcgaggct ctcgaagtcc aggaggctgg agaacctgat
     3241 cgctcagctc ccaggcgaga agaagaacgg cctgttcggg aacctcatcg ctctcagcct
     3301 ggggctcacc ccgaacttca agtcgaactt cgatctcgct gaggacgcca agctgcaact
     3361 ctccaaggac acctacgacg atgacctcga taacctcctg gcccagatcg gcgatcaata
     3421 cgcggacctg ttcctcgctg ccaagaacct gtcggacgcc atcctcctgt cagatatcct
     3481 ccgcgtgaac accgagatca cgaaggctcc actctctgcc tccatgatca agcgctacga
     3541 cgagcaccat caggatctga ccctcctgaa ggcgctggtc cgccaacagc tcccggagaa
     3601 gtacaaggag attttcttcg atcagtcgaa gaacggctac gctgggtaca tcgacggcgg
     3661 ggcctcacaa gaggagttct acaagttcat caagccaatc ctggagaaga tggacggcac
     3721 ggaggagctc ctggtgaagc tcaacaggga ggacctcctg cggaagcaga gaaccttcga
     3781 taacggcagc atcccccacc aaatccatct cggggagctg cacgccatcc tgagaaggca
     3841 agaggacttc taccctttcc tcaaggataa ccgggagaag atcgagaaga tcctgacctt
     3901 cagaatccca tactacgtcg gccctctcgc gcgggggaac tcaagattcg cttggatgac
     3961 ccgcaagtct gaggagacca tcacgccgtg gaacttcgag gaggtggtgg acaagggcgc
     4021 tagcgctcag tcgttcatcg agaggatgac caacttcgac aagaacctgc ccaacgagaa
     4081 ggtgctccct aagcactcgc tcctgtacga gtacttcacc gtctacaacg agctcacgaa
     4141 ggtgaagtac gtcaccgagg gcatgcgcaa gccagcgttc ctgtccgggg agcagaagaa
     4201 ggctatcgtg gacctcctgt tcaagaccaa ccggaaggtc acggttaagc aactcaagga
     4261 ggactacttc aagaagatcg agtgcttcga ttcggtcgag atcagcggcg ttgaggaccg
     4321 cttcaacgcc agcctcggga cctaccacga tctcctgaag atcatcaagg ataaggactt
     4381 cctggacaac gaggagaacg aggatatcct ggaggacatc gtgctgaccc tcacgctgtt
     4441 cgaggacagg gagatgatcg aggagcgcct gaagacgtac gcccatctct tcgatgacaa
     4501 ggtcatgaag caactcaagc gccggagata caccggctgg gggaggctgt cccgcaagct
     4561 catcaacggc atccgggaca agcagtccgg gaagaccatc ctcgacttcc tcaagagcga
     4621 tggcttcgcc aacaggaact tcatgcaact gatccacgat gacagcctca ccttcaagga
     4681 ggatatccaa aaggctcaag tgagcggcca gggggactcg ctgcacgagc atatcgcgaa
     4741 cctcgctggc tcccccgcga tcaagaaggg catcctccag accgtgaagg ttgtggacga
     4801 gctcgtgaag gtcatgggcc ggcacaagcc tgagaacatc gtcatcgaga tggccagaga
     4861 gaaccaaacc acgcagaagg ggcaaaagaa ctctagggag cgcatgaagc gcatcgagga
     4921 gggcatcaag gagctggggt cccaaatcct caaggagcac ccagtggaga acacccaact
     4981 gcagaacgag aagctctacc tgtactacct ccagaacggc agggatatgt acgtggacca
     5041 agagctggat atcaaccgcc tcagcgatta cgacgtcgat catatcgttc cccagtcttt
     5101 cctgaaggat gactccatcg acaacaaggt cctcaccagg tcggacaaga accgcggcaa
     5161 gtcagataac gttccatctg aggaggtcgt taagaagatg aagaactact ggaggcagct
     5221 cctgaacgcc aagctgatca cgcaaaggaa gttcgacaac ctcaccaagg ctgagagagg
     5281 cgggctctca gagctggaca aggccggctt catcaagcgg cagctggtcg agaccagaca
     5341 aatcacgaag cacgttgcgc aaatcctcga ctctcggatg aacacgaagt acgatgagaa
     5401 cgacaagctg atcagggagg ttaaggtgat caccctgaag tctaagctcg tctccgactt
     5461 caggaaggat ttccagttct acaaggttcg cgagatcaac aactaccacc atgcccatga
     5521 cgcttacctc aacgctgtgg tcggcaccgc tctgatcaag aagtacccaa agctggagtc
     5581 cgagttcgtg tacggggact acaaggttta cgatgtgcgc aagatgatcg ccaagtcgga
     5641 gcaagagatc ggcaaggcta ccgccaagta cttcttctac tcaaacatca tgaacttctt
     5701 caagaccgag atcacgctgg ccaacggcga gatccggaag agaccgctca tcgagaccaa
     5761 cggcgagacg ggggagatcg tgtgggacaa gggcagggat ttcgcgaccg tccgcaaggt
     5821 tctctccatg ccccaggtga acatcgtcaa gaagaccgag gtccaaacgg gcgggttctc
     5881 aaaggagtct atcctgccta agcggaacag cgacaagctc atcgccagaa agaaggactg
     5941 ggacccaaag aagtacggcg ggttcgacag ccctaccgtg gcctactcgg tcctggttgt
     6001 ggcgaaggtt gagaagggca agtccaagaa gctcaagagc gtgaaggagc tcctggggat
     6061 caccatcatg gagaggtcca gcttcgagaa gaacccaatc gacttcctgg aggccaaggg
     6121 ctacaaggag gtgaagaagg acctgatcat caagctcccg aagtactctc tcttcgagct
     6181 ggagaacggc aggaagagaa tgctggcttc cgctggcgag ctccagaagg ggaacgagct
     6241 cgcgctgcca agcaagtacg tgaacttcct ctacctggct tcccactacg agaagctcaa
     6301 gggcagcccg gaggacaacg agcaaaagca gctgttcgtc gagcagcaca agcattacct
     6361 cgacgagatc atcgagcaaa tctccgagtt cagcaagcgc gtgatcctcg ccgacgcgaa
     6421 cctggataag gtcctctccg cctacaacaa gcaccgggac aagcccatca gagagcaagc
     6481 ggagaacatc atccatctct tcaccctgac gaacctcggc gctcctgctg ctttcaagta
     6541 cttcgacacc acgatcgatc ggaagagata cacctccacg aaggaggtcc tggacgcgac
     6601 cctcatccac cagtcgatca ccggcctgta cgagacgagg atcgacctct cacaactcgg
     6661 cggggataag agacccgcag caaccaagaa ggcagggcaa gcaaagaaga agaagacgcg
     6721 tgactccggc ggcagcacca acctgtccga catcatcgag aaggagacgg gcaagcaact
     6781 cgtgatccag gagagcatcc tcatgctgcc agaggaggtg gaggaggtca tcggcaacaa
     6841 gccagagtcc gacatcctgg tgcacaccgc ctacgacgag tccaccgacg agaacgtcat
     6901 gctcctgacc agcgacgccc cagagtacaa gccatgggcc ctcgtcatcc aggacagcaa
     6961 cggggagaac aagatcaaga tgctgtcggg ggggagccca aagaagaagc ggaaggtgta
     7021 gtgtacaagt aacccgggaa ttcggtacgc tgaaatcacc agtctctctc tacaaatcta
     7081 tctctctcta ttttctccat aaataatgtg tgagtagttt cccgataagg gaaattaggg
     7141 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     7201 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa
     7261 tccagatctc ctaaagtccc tatagatctt tgtcgtgaat ataaaccaga cacgagacga
     7321 ctaaacctgg agcccagacg ccgttcgaag ctagaagtac cgcttaggca ggaggccgtt
     7381 agggaaaaga tgctaaggca gggttggtta cgttgactcc cccgtaggtt tggtttaaat
     7441 atgatgaagt ggacggaagg aaggaggaag acaaggaagg ataaggttgc aggccctgtg
     7501 caaggtaaga agatggaaat ttgatagagg tacgctacta tacttatact atacgctaag
     7561 ggaatgcttg tatttatacc ctataccccc taataacccc ttatcaattt aagaaataat
     7621 ccgcataagc ccccgcttaa aaattggtat cagagccatg aataggtcta tgaccaaaac
     7681 tcaagaggat aaaacctcac caaaatacga aagagttctt aactctaaag ataaaagatc
     7741 tttcaagatc aaaactagtt ccctcacacc ggtgacgggg atcgcatgcg atatctcgag
     7801 atctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7861 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7921 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7981 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8041 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8101 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8161 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8221 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8281 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8341 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8401 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8461 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8521 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8581 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8641 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8701 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8761 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8821 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8881 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8941 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9001 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9061 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9121 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9181 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9241 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9301 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9361 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9421 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9481 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9541 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9601 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9661 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9721 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9781 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9841 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9901 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9961 aaaagtgcca cctgccagtg ccaagctaat tc
//
