LOCUS       MPRA_Empty_Vector        2550 bp    DNA     circular SYN 26-JUN-2023
DEFINITION  MPRA Empty Vector.
ACCESSION   -
KEYWORDS    .
SOURCE      Firefly luciferase reporter vector pGL4.23[luc2/minP]
  ORGANISM  Artificial
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Swanson,B., Kopish,K. and McNamara,B.
  TITLE     pGL4 Luciferase reporter Vectors Technical Manual, TM259
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Swanson,B., Kopish,K. and McNamara,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2006) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     misc_feature    1..70
                     /note="multiple cloning region"
     polyA_signal    95..316
                     /note="SV40 late poly(A) signal"
     rep_origin      641
                     /note="ColE1-derived plasmid replication origin"
     CDS             complement(1432..2292)
                     /gene="Amp(r)"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="ABI94440.1"
                     /db_xref="GI:115342876"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1432..2292)
                     /gene="Amp(r)"
     polyA_signal    2397..2550
                     /note="poly(A) signal/transcriptional pause site"
     primer_bind     2499..2518
                     /note="RVprimer3 binding region"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag
      121 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
      181 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      241 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      301 ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga gagccttcaa
      361 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
      421 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca
      481 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      541 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      601 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      661 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      721 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      781 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      841 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      901 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      961 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1021 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1081 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1141 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1201 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1261 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1321 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1381 atgagtaaac ttggtctgac agcggccgca aatgctaaac cactgcagtg gttaccagtg
     1441 cttgatcagt gaggcaccga tctcagcgat ctgcctattt cgttcgtcca tagtggcctg
     1501 actccccgtc gtgtagatca ctacgattcg tgagggctta ccatcaggcc ccagcgcagc
     1561 aatgatgccg cgagagccgc gttcaccggc ccccgatttg tcagcaatga accagccagc
     1621 agggagggcc gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc agtctatgag
     1681 ctgctgtcgt gatgctagag taagaagttc gccagtgagt agtttccgaa gagttgtggc
     1741 cattgctact ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt tcaactctgg
     1801 ttcccagcgg tcaagccggg tcacatgatc acccatatta tgaagaaatg cagtcagctc
     1861 cttagggcct ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat
     1921 ggcagcacta cacaattctc ttaccgtcat gccatccgta agatgctttt ccgtgaccgg
     1981 cgagtactca accaagtcgt tttgtgagta gtgtatacgg cgaccaagct gctcttgccc
     2041 ggcgtctata cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg
     2101 gaatcgttct tcggggcgga aagactcaag gatcttgccg ctattgagat ccagttcgat
     2161 atagcccact cttgcaccca gttgatcttc agcatctttt actttcacca gcgtttcggg
     2221 gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg
     2281 ttggatgctc atactcgtcc tttttcaata ttattgaagc atttatcagg gttactagta
     2341 cgtctctcaa ggataagtaa gtaatattaa ggtacgggag gtattggaca ggccgcaata
     2401 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa
     2461 catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca
     2521 gtgcaagtgc aggtgccaga acatttctct
//
