LOCUS       265_S16_ChR1_pcD        7179 bp DNA     circular SYN 25-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Joe
  TITLE     Direct Submission
  JOURNAL   Exported Jun 27, 2023 from SnapGene 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7179
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        3..306
                     /label=CMV enhancer
     promoter        307..510
                     /label=CMV promoter
     primer_bind     462..480
                     /label=CMV-F
     primer_bind     477..501
                     /label=pCEP4for
     primer_bind     490..509
                     /label=EGFP-N1-F
     primer_bind     555..574
                     /label=T7
     primer_bind     555..574
                     /label=T7prom
     primer_bind     673..710
                     /label=S16_ChR1 f
     primer_bind     complement(675..695)
                     /label=pcDNA3.1-PgV-Rho_r
     primer_bind     complement(675..692)
                     /label=pcDNA3.1 r
     CDS             693..1622
                     /codon_start=1
                     /label=S16_ChR
                     /translation="MSGGGKTIFTCLFLCAAWAPCVDATNATFTCPSDAEATSGIGVDM
                     ACRIFLICVCVLQFCFFLYQRIRYNRAGIDTLWVVLAELIVYSMYVATKASGANPAAIV
                     EGCRSVPLLRYASWLVTCPVQLITVSILSARSKDKQHDVMELTVILILDQVMIICGILG
                     KAFGGRSANVNWMCFAIAAACLCSIFRKLAATLKNAHASFPDEAKPYLHVISVCFFVGW
                     PFFPTAWLIEEAGLSTPTIEAVLHVFGDLIAKNLFLAATWTLRWRVLAQKDGGTTRIAT
                     KLAMPGESQHIAVVDRDPVYVKLLTHLLE"
     protein_bind    1134..1145
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(1605..1642)
                     /label=S16_ChR1 r
     primer_bind     1623..1640
                     /label=pcDNA3.1-PgV-Rho_f
     primer_bind     1623..1640
                     /label=pcDNA3.1 f
     CDS             1635..1694
                     /codon_start=1
                     /label=TS
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             1704..2399
                     /codon_start=1
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     CDS             2400..2423
                     /codon_start=1
                     /label=ER
                     /translation="FCYENEV"
     primer_bind     complement(2463..2482)
                     /label=pcDNA3.1-R
     primer_bind     complement(2500..2519)
                     /label=UniV5his-R
     primer_bind     complement(2594..2613)
                     /label=F1-oriR
     rep_origin      2758..3064
                     /label=F1 ori
     primer_bind     3468..3485
                     /label=SV40-Do
     CDS             3582..4373
                     /label=Kan/neoR
     primer_bind     complement(4552..4574)
                     /label=EGFP-C1-R
     primer_bind     4623..4643
                     /label=SV40
     primer_bind     complement(4705..4728)
                     /label=M13-24R-BLUE
     primer_bind     complement(4713..4733)
                     /label=M13-rev
     primer_bind     complement(4713..4731)
                     /label=BluescriptR
     primer_bind     complement(4716..4733)
                     /label=M13-29R
     primer_bind     complement(4717..4733)
                     /label=M13-27R
     primer_bind     complement(4730..4750)
                     /label=M13-24R
     primer_bind     complement(4731..4750)
                     /label=pQE-F
     misc_binding    complement(4739..4761)
                     /label=LacO
     promoter        complement(4765..4795)
                     /label=lac promoter
     primer_bind     complement(4778..4801)
                     /label=MKSF
     primer_bind     complement(4806..4825)
                     /label=M13rev2
     primer_bind     complement(5086..5105)
                     /label=ASK-FN2
     CDS             complement(5878..6537)
                     /label=AmpR
     primer_bind     6824..6843
                     /label=sCOS1F
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
      541 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcgtttaaac
      601 gggccctcta gactcgagcg gccgccactg tgctggatat ctgcagaatt ccaccacact
      661 ggactagtgg atccccgggt accggtgcca ccatgtctgg tggaggtaag acaattttca
      721 catgtctttt tctgtgtgcc gcctgggctc cttgtgtgga cgccaccaac gcaactttca
      781 cttgtccttc agacgcagaa gcaacaagtg gtatcggagt ggatatggca tgcagaatct
      841 ttttgatttg tgtctgcgtg ctccaattct gtttttttct gtatcaacgc atacggtaca
      901 atagggcagg tatagatacc ctgtgggtag tccttgctga attgatcgtg tatagtatgt
      961 atgttgctac caaagcctct ggcgctaacc cagccgcaat agttgagggg tgtcgctctg
     1021 tgccactgtt gcgatatgca agctggttgg ttacttgccc agtccaactg attaccgtct
     1081 ctatcctttc cgccagatca aaagacaagc agcatgatgt gatggaattg actgtcatcc
     1141 tcatcttgga tcaagttatg atcatttgtg gaatcttggg aaaagccttc ggtggccgca
     1201 gtgccaacgt gaactggatg tgtttcgcaa ttgccgccgc atgcctgtgc agcattttta
     1261 gaaaactggc tgccaccctg aaaaacgctc atgcatcttt tcccgacgag gctaaaccct
     1321 atcttcatgt aatcagcgta tgtttctttg taggctggcc cttttttcct acagcctggc
     1381 ttattgaaga agccgggttg tctacaccaa ctatagaggc cgtgctgcat gtcttcggtg
     1441 acctcatcgc taaaaacttg tttctggctg ccacttggac attgagatgg agagttttgg
     1501 ctcagaagga tggtggtact acccgcattg ccacaaagct ggctatgcct ggcgagagtc
     1561 agcatattgc cgttgtggat cgagacccag tatatgtaaa attgctgact catttgttgg
     1621 aagcggcacc ggtgaagagc aggatcacca gcgagggcga gtacatcccc ctggaccaga
     1681 tcgacatcaa cgtggcggta cccatggtga gcaagggcga ggcagtgatc aaggagttca
     1741 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
     1801 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     1861 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
     1921 tcaccaagca ccccgccgac atccccgact actataagca gtccttcccc gagggcttca
     1981 agtgggagcg cgtgatgaac ttcgaggacg gcggcgccgt gaccgtgacc caggacacct
     2041 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcaccaac ttccctcctg
     2101 acggccccgt aatgcagaag aagacaatgg gctgggaagc gtccaccgag cggttgtacc
     2161 ccgaggacgg cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
     2221 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
     2281 cctacaacgt cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
     2341 aacagtacga acgctccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     2401 tctgctacga gaacgaggtg taatgagaat tcgatatcaa gcttaagttt aaaccgctga
     2461 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     2521 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     2581 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     2641 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     2701 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     2761 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2821 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2881 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2941 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3001 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3061 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3121 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     3181 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3241 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     3301 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     3361 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3421 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     3481 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     3541 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     3601 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     3661 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     3721 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     3781 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     3841 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     3901 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     3961 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4021 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     4081 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     4141 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     4201 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     4261 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     4321 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     4381 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     4441 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     4501 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     4561 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4621 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     4681 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4741 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4801 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4861 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4921 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4981 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5041 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5101 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5161 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5221 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5281 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5341 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5401 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5461 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5521 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5581 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5641 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5701 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5761 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5821 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5881 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5941 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6001 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6061 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6121 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6181 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6241 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6301 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6361 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6421 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6481 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6541 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6601 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6661 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6721 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6781 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6841 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     6901 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     6961 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     7021 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     7081 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     7141 attacggggt cattagttca tagcccatat atggagttc
//
