LOCUS       266_GpChR1_pcDNA        7155 bp DNA     circular SYN 25-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Joe
  TITLE     Direct Submission
  JOURNAL   Exported Jun 27, 2023 from SnapGene 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7155
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        2..305
                     /label=CMV enhancer
     promoter        306..509
                     /label=CMV promoter
     primer_bind     461..479
                     /label=CMV-F
     primer_bind     476..500
                     /label=pCEP4for
     primer_bind     489..508
                     /label=EGFP-N1-F
     primer_bind     554..573
                     /label=T7
     primer_bind     554..573
                     /label=T7prom
     primer_bind     672..713
                     /label=GpChR1 f
     primer_bind     complement(674..694)
                     /label=pcDNA3.1-PgV-Rho_r
     primer_bind     complement(674..691)
                     /label=pcDNA3.1 r
     CDS             692..1597
                     /codon_start=1
                     /label=GpChR1
                     /translation="MLALDEVATFTQWSVMAISFLQFAFLVDRFRQGQCFVEALYVIMV
                     EFIGYGLNVAGWYTVNLEDGRTFYIGRYVSWILTSPVLLIIVCNLHAEARIEPRRTLFC
                     SVMFPLMTALGGAAALIEIIWVRVALFLLSCVICGLIFRVVHICRTKSLIVVGGNDTAR
                     RLIDRITWLFYVTWCMYPTFVLLGPEFFDVVPAAGVAVWAAVTDLFSKNLFGLLCWFLR
                     WQVIEPRGRRVVGLVKQFSRLNSMLNNVEDTPRPSPHGKHVVLNTGSAFADTPEGVQLT
                     APSPSPSPRLGSLPPPALPMP"
     primer_bind     complement(1580..1617)
                     /label=GpChR1 r
     primer_bind     1598..1615
                     /label=pcDNA3.1-PgV-Rho_f
     primer_bind     1598..1615
                     /label=pcDNA3.1 f
     CDS             1610..1669
                     /codon_start=1
                     /label=TS
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             1679..2374
                     /codon_start=1
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     CDS             2375..2398
                     /codon_start=1
                     /label=ER
                     /translation="FCYENEV"
     primer_bind     complement(2438..2457)
                     /label=pcDNA3.1-R
     primer_bind     complement(2475..2494)
                     /label=UniV5his-R
     primer_bind     complement(2569..2588)
                     /label=F1-oriR
     rep_origin      2733..3039
                     /label=F1 ori
     primer_bind     3443..3460
                     /label=SV40-Do
     CDS             3557..4348
                     /label=Kan/neoR
     primer_bind     complement(4527..4549)
                     /label=EGFP-C1-R
     primer_bind     4598..4618
                     /label=SV40
     primer_bind     complement(4680..4703)
                     /label=M13-24R-BLUE
     primer_bind     complement(4688..4708)
                     /label=M13-rev
     primer_bind     complement(4688..4706)
                     /label=BluescriptR
     primer_bind     complement(4691..4708)
                     /label=M13-29R
     primer_bind     complement(4692..4708)
                     /label=M13-27R
     primer_bind     complement(4705..4725)
                     /label=M13-24R
     primer_bind     complement(4706..4725)
                     /label=pQE-F
     misc_binding    complement(4714..4736)
                     /label=LacO
     promoter        complement(4740..4770)
                     /label=lac promoter
     primer_bind     complement(4753..4776)
                     /label=MKSF
     primer_bind     complement(4781..4800)
                     /label=M13rev2
     primer_bind     complement(5061..5080)
                     /label=ASK-FN2
     CDS             complement(5853..6512)
                     /label=AmpR
     primer_bind     6799..6818
                     /label=sCOS1F
ORIGIN
        1 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
       61 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      121 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      181 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      241 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      301 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      361 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      421 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      481 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
      541 ggcttatcga aattaatacg actcactata gggagaccca agctggctag cgtttaaacg
      601 ggccctctag actcgagcgg ccgccactgt gctggatatc tgcagaattc caccacactg
      661 gactagtgga tccccgggta ccggtgccac catgctcgct cttgatgaag tcgccacctt
      721 tacccaatgg tctgtgatgg caataagttt tctgcagttc gcattccttg tcgacagatt
      781 taggcagggg cagtgtttcg ttgaggctct gtacgtgata atggtggaat ttatcggcta
      841 cgggttgaac gtggcagggt ggtatactgt aaacctggag gacggacgaa ccttttatat
      901 tggccgctac gtttcctgga tccttacatc ccctgtcctc cttatcatag tatgtaacct
      961 tcacgccgag gctcgcattg aacccagaag gacattgttc tgctccgtga tgtttccctt
     1021 gatgactgcc ctcgggggcg cagccgcctt gatcgaaatt atatgggtaa gagttgcact
     1081 gttccttctt agctgtgtta tctgtgggct gatttttaga gtagttcaca tatgccgaac
     1141 aaaaagtctg attgtcgtgg gcggaaacga cactgctaga cgcctcattg accgaataac
     1201 ctggcttttt tacgtgacct ggtgtatgta tccaaccttt gtgctcttgg ggcccgaatt
     1261 tttcgatgta gtgcctgccg caggagtcgc cgtctgggcc gcagtcactg atcttttcag
     1321 caaaaacctg ttcggattgt tgtgttggtt tcttcggtgg caggttattg aacccagagg
     1381 taggagagtc gtggggcttg tgaagcaatt cagtcggctg aattcaatgc tcaataatgt
     1441 cgaagatacc cccaggccaa gcccacacgg caagcacgta gttttgaata ccggcagtgc
     1501 ttttgctgac actcctgagg gagtgcagtt gaccgcccca agtccttctc catcaccacg
     1561 ccttggatct ttgccccctc cagctctgcc catgcctgcg gcaccggtga agagcaggat
     1621 caccagcgag ggcgagtaca tccccctgga ccagatcgac atcaacgtgg cggtacccat
     1681 ggtgagcaag ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg
     1741 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     1801 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat
     1861 cctgtcccct cagttcatgt acggctccag ggccttcacc aagcaccccg ccgacatccc
     1921 cgactactat aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     1981 ggacggcggc gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta
     2041 caaggtgaag ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac
     2101 aatgggctgg gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga
     2161 cattaagatg gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac
     2221 ctacaaggcc aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc gcaagttgga
     2281 catcacctcc cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgagggccg
     2341 ccactccacc ggcggcatgg acgagctgta caagttctgc tacgagaacg aggtgtaatg
     2401 agaattcgat atcaagctta agtttaaacc gctgatcagc ctcgactgtg ccttctagtt
     2461 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     2521 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     2581 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     2641 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     2701 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2761 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     2821 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2881 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     2941 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3001 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     3061 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3121 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     3181 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3241 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3301 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3361 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3421 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3481 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     3541 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3601 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     3661 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3721 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     3781 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     3841 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     3901 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     3961 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4021 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4081 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4141 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4201 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4261 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4321 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     4381 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4441 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     4501 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     4561 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4621 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     4681 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4741 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4801 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4861 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4921 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4981 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5041 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5101 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5161 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5221 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5281 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5341 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5401 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5461 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5521 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5581 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt
     5641 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5701 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5761 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5821 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5881 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5941 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6001 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6061 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6121 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6181 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6241 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6301 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6361 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6421 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6481 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6541 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6601 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6661 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6721 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6781 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6841 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     6901 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     6961 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     7021 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     7081 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7141 catatatgga gttcc
//
