LOCUS       Exported               10260 bp ds-DNA     circular SYN 27-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Merlin Brychcy
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 27, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10260
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..22
                     /label=GG1D
                     /note="GG1D"
     protein_bind    123..144
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        159..189
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    197..213
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             231..242
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             249..926
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /label=monomeric derivative of DsRed (Campbell et al.,
                     /note="mRFP1"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      971..1042
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1058..1085
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    1092..1113
                     /label=GG1D
                     /note="GG1D1"
     primer_bind     1114..1139
                     /label=ori_pstI_f
     regulatory      1120..1149
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     CDS             1273..1833
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88093.1"
                     /translation="MLTRWDNDQSSLSIYHREIKEEQQEQQRKQQQIERETKQKQEDDK
                     KKRQDYERYLSKYGYVKDAEYDHISDHVASDISMFTRLQAQAWASNDMQEYIRCSKQKY
                     EQISDRIAYERDLKKIGQLSRILDKDRQALADILPQLMPYYEQMQQAVNKRKILLENER
                     QPSFKPRYDQEQPKPKKDNDLTF"
     regulatory      1817..1844
                     /regulatory_class="promoter"
                     /label=putative antitoxin-toxin module-predicted promoter
                     /note="putative antitoxin-toxin module-predicted promoter"
     CDS             1878..2171
                     /codon_start=1
                     /product="putative PaaA2-like antitoxin"
                     /label=putative PaaA2-like antitoxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88095.1"
                     /translation="MTEATFTFRVDHDLKQEFSSLAKTVDRSGAQLIRDFMRDFVKKQQ
                     EAADYDKWFKQQVQIGLNEANAGKLIPHEDVKAEFAARRAATLAKLAAKNED"
     CDS             2161..2430
                     /codon_start=1
                     /product="putative ParE2-like toxin"
                     /label=putative ParE2-like toxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88096.1"
                     /translation="MKIEWTETARQDRRNIYDYLEERNPIAAIEIDDLIEEKTDLLVDN
                     RLMGRTGRQKDTRELVIHPHYVVVYDITDIIRILRVLHTSQEWS"
     regulatory      2194..3530
                     /regulatory_class="replication_regulatory_region"
                     /note="minimal origin of replication for Acinetobacter
                     sp."
     regulatory      3703..3729
                     /regulatory_class="promoter"
                     /label=putative RCR protein gene predicted promoter
                     /note="putative RCR protein gene predicted promoter"
     regulatory      4105..4125
                     /regulatory_class="terminator"
                     /note="bidirectional Rho-independent transcriptional
                     terminator"
     CDS             complement(4144..4413)
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88098.1"
                     /translation="MVKKTAMELGQMLDEEKEKLERQEKARKDLADAVVKGREQKQARS
                     DDAKRKILIGAYLLKKHDNDIKKLVEQNPDFIGYIRENDKHLFS"
     regulatory      complement(4446..4471)
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     regulatory      4582..4608
                     /regulatory_class="promoter"
                     /label=putative nickase-like gene predicted promoter
                     /note="putative nickase-like gene predicted promoter"
     CDS             4698..5039
                     /codon_start=1
                     /product="putative nickase-like protein"
                     /label=putative nickase-like protein
                     /note="may be involved in rolling circle replication
                     (RCR)"
                     /protein_id="AUJ88099.1"
                     /translation="MKMAIYHCEMQNISRSDGRSIVACAAYRAGEKLYCDTYGKEQDYT
                     KKTGIEYTQIFAPTGASPDMLDRQTLWNRVEQSELKKNGDIKQEARLAKEVEIALPHEL
                     DKTQRQATC"
     rep_origin      complement(5222..5810)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5981..6841)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6842..6946)
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     terminator      7036..7079
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    7082..7097
                     /label=BioBrick prefix
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that start with ""ATG"""
     protein_bind    7098..7117
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      7181..7212
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(7233..8183)
                     /codon_start=1
                     /label=klaC
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV
                     TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF
                     TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP
                     SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG
                     SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG
                     AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP"
     CDS             complement(8183..9316)
                     /codon_start=1
                     /label=klaB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE
                     VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN
                     RSRLPLIGPLIDRSRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA
                     VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD
                     LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL
                     ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ
                     EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV"
     primer_bind     complement(8380..8399)
                     /label=pMO130-TelR_pointBsmBI_r
     misc_feature    8400..10254
                     /label=tellurite cassette
     primer_bind     8400..8415
                     /label=pMo130-TelR_pointBsmBI_f
     CDS             complement(9337..10095)
                     /codon_start=1
                     /label=klaA
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=98.4"
                     /note="/other=CDS"
                     /translation="SVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNLMEHG
                     AVAALADKISQIVAKLVDADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQLLDEA
                     EGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEFDKPR
                     ERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLALITT
                     KNMNPAMVAEAAKAHQALMRSLSQSLEGINQ"
     RBS             10113..10135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    10227..10254
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    10255..10258
                     /label=BioBrick prefix
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcagaa gctatagaga cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      181 cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga
      241 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
      301 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
      361 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
      421 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
      481 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
      541 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
      601 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
      661 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
      721 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
      781 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
      841 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
      901 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
      961 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1021 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1081 ctgcgtttat aggtctcatg ttttagagag acgctgcagt tgcaagacaa tatcgaccgc
     1141 ttgcagcttg ataaagtgat caaagaggga cgagcagcta cagcactgct aagggagatc
     1201 ggagagccac tgccgccacc agtgaaagtg aaaacaggtt ggtttagtgc tgattattac
     1261 ggctttgagg acatgctcac acgttgggat aatgatcaat ctagcctttc catttatcac
     1321 agagagataa aagaggagca acaggaacag caacgaaaac aacagcagat agaaagagaa
     1381 actaaacaaa aacaggaaga tgacaaaaag aaacggcagg actatgaaag atatttatca
     1441 aaatacggct atgtcaaaga tgctgagtat gaccatatca gcgaccatgt tgcttcagac
     1501 atttccatgt ttacacgtct tcaggcgcag gcatgggcaa gtaacgatat gcaagagtac
     1561 ataaggtgtt caaagcaaaa atacgagcag atcagcgaca gaatagccta tgagcgtgac
     1621 ttgaaaaaaa tcgggcaatt gagccgtatc ctggataaag acagacaggc acttgctgat
     1681 attttaccgc agttgatgcc ttactatgag cagatgcaac aggctgttaa taaacggaaa
     1741 attttacttg agaatgagcg acagccatca tttaaaccac gttatgacca agaacagcct
     1801 aagccgaaaa aagacaatga cctaactttt taatgtgtta caatagcatt caaatgtaac
     1861 acattggagt gaaaaacatg acagaagcga cttttacctt tcgggtagac catgacctaa
     1921 agcaagaatt ttcaagcctt gctaagactg ttgatcggtc gggggcgcag ctaatccgtg
     1981 acttcatgcg tgactttgtt aagaagcaac aggaagccgc agactatgac aaatggttca
     2041 agcagcaggt acagatcggc ttaaatgaag ccaatgcagg caagttgatt ccacatgagg
     2101 acgtaaaggc agagtttgcc gctagacgtg ctgcaacatt ggcgaaactg gcagcgaaga
     2161 atgaagattg agtggactga aacagctcgc caagatcgta gaaatatcta tgattatctt
     2221 gaagaacgca accctatagc agctattgaa attgatgatt taattgaaga aaagacagat
     2281 ttacttgttg ataatcgact gatggggcgc acaggcagac agaaagatac tagggagtta
     2341 gtgatacatc cgcattatgt ggttgtatat gacatcactg atataatacg gatactcaga
     2401 gtgctacaca catcgcagga gtggtcatga cttactcatg tactttggat tatttagtgt
     2461 tataaaatcc tgatttataa attttttttg ttaaaaaaga taaaagcccc ttgcaattgc
     2521 ttggggcttt accgtaattt atggggtaca gatcttcgat actgacatat cggcaatcga
     2581 aagcattaag gtttgacgac cgctaatgat ttcaccacag gggcttaatg tacctgtctt
     2641 aaattctaag gttttaactc gctttgtcaa gcatagaccc caaaaattta gccaatgtct
     2701 gtaactcaat ctgtccatgt gtgggtgatg aggtacagtg acgctagcac acatcggaaa
     2761 aacgctatta ctaggggaac tgaacagagt agcggacgca atgagtagtc atttaattgg
     2821 cggttatgag cgtgttcagg cggtgctatc aatcgtaatc ataacagtgg cagcttgata
     2881 cagtgatgtc atccctgatg cgaaagcgac cgaccgacgg tacatcgaat gggaatactt
     2941 tagggtgatt tttaagaatc gctctagggt gagtatttcc cattcagctc tgctccctcc
     3001 ctctggtact ttaatcaaaa gcactactaa acatatgttt ttaaataaaa aatattgata
     3061 tagagataat attagtaaga ataattaaac aattgaatat agataaatca ttgttaaata
     3121 aagattaatt attaaaatga atgtatactt atatataaat caatgattta aaatatttga
     3181 taaagaaaac ttttcaaaaa aaatataatt gagattgtgt catttcggtc aattcttaat
     3241 atgttccacg caagttttag ctatggtgct aaacagaaat ttgctgaaaa agaacttttc
     3301 actgaactgg ttaaaatgta agcagcctga gagccgccaa aaattttaaa aacaaaccgc
     3361 cttaatcatc ttcaaaaaat acctctaaaa cctcaccatt tgcgttttaa gacccatatt
     3421 tcatcctgcc cttatgttcc catgctgata gctataaagt gtctgtaatc gcttcctatg
     3481 acgttctagg ctgttgataa cttttggaac aacgcaaaat gttaaaatcc gatcattttt
     3541 taacctagtt attttcgtta cagggtaact ttggtagtat tatttcaata tttagttgtt
     3601 acaggataac aaaatatatg ttacagggta attaatttag cgttacagga taactatagt
     3661 tacagggtaa cgttaaatag ttatcctgta atttaaaaat agttgcagga tagcataaat
     3721 ttttacaaat aaattttata aaaagttatc ctgtaactaa aaacaatgtt atcctgtaac
     3781 tatgatatac tatatgggag ttaaagacat gaaagacccg aacgatcaga aaacacagga
     3841 catgctaaaa gagccgccaa aaaccaatgc cgaacgacaa aaagcatatc gtgaaaaacg
     3901 caaaagcctt gatagtcaac gtttggaggt gtttatagat aagggtgtgt cggatatgct
     3961 tgcggacatg gtgggcgcag caggggagag ccaaaaagcc attttgaccg cattgattga
     4021 gaaagagtat aagcggctgt atgcagtgaa aaatagcaag ctaaaaaagt acattgattc
     4081 aaaaagtaat gcagataaaa aagaaaccct cgatttatga gggtttttta taactactaa
     4141 tgcctatgaa aataagtgct tatcattttc tctaatgtac ccaataaaat cggggttttg
     4201 ttccacaagt ttcttgatgt cattatcatg tttcttcagt aagtaagccc cgatcaggat
     4261 cttgcgtttt gcatcgtctg accttgcctg tttttgctct cgacctttca ccacagcgtc
     4321 agctagatcc tttcttgcct tttcctgacg ttccaatttt tccttttctt catcgagcat
     4381 ttgacccaat tccatagctg tttttttgac catttttaat gcctgtgtct caacctattt
     4441 tccgaagtat gaaccatcat caatgtgaaa agtgttctac ttcaaaaact cgacttacat
     4501 caagatccga gcgcagcgag caaaatcaaa aaacaaagtc aaagccttat tgctcttgct
     4561 tttgctttta gcctcgcaga gttcccgaag ggcgcactta cgcaaaattt ttgctacgcc
     4621 aaattttgca agtacggtca gggaaacccc gacaccccaa ccgcccaaaa cttggggcgg
     4681 ctgttaataa acaggtgatg aaaatggcaa tttaccattg tgaaatgcag aacatttcga
     4741 ggtcagatgg tcgctcaatc gtggcatgtg cagcataccg agcaggcgaa aaattgtact
     4801 gtgatacgta cggaaaagag caggactaca ccaaaaaaac aggcattgaa tacacccaaa
     4861 tttttgcccc aactggggca agtcctgaca tgttagatcg tcaaacccta tggaatcggg
     4921 tagagcaatc cgaactaaaa aagaacggtg acatcaaaca ggaggcaaga ttagcaaagg
     4981 aagtagagat agcattgccg catgaactgg ataagacaca gcgtcaggca acttgttaac
     5041 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5101 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5161 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5221 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5281 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5341 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5401 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5461 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5521 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5581 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5641 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     5701 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5761 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5821 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5881 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5941 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6001 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6061 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6121 gagagccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6181 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6241 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6301 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6361 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6421 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6481 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6541 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6601 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6661 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6721 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6781 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6841 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6901 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6961 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     7021 gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct
     7081 ggaattcgcg gccgcttcta gaaagtatag gaacttcaga gcgcttttga agctgatgtg
     7141 cttaaaaact tactcaatgg aattagctcc tcgagcagaa agtcaaaagc ctccgaccgg
     7201 aggcttttga cttgaggggg atcgatccct tatggctctg cacccggctc catcaccaac
     7261 aggtcgcgca cgcgcttcac tcggttgcgg atcgacactg ccagcccaac aaagccggtt
     7321 gccgccgccg ccaggatcgc gccgatgatg ccggccacac cggccatcgc ccaccaggtc
     7381 gccgccttcc ggttccattc ctgctggtac tgcttcgcaa tgctggacct cggctcacca
     7441 taggctgacc gctcgatggc gtatgccgct tctccccttg gcgtaaaacc cagcgccgca
     7501 ggcggcattg ccatgctgcc cgccgctttc ccgaccacga cgcgcgcacc aggcttgcgg
     7561 tccagacctt cggccacggc gagctgcgca aggacataat cagccgccga cttggctcca
     7621 cgcgcctcga tcagctcttg cactcgcgcg aaatccttgg cctccacggc cgccatgaat
     7681 cgcgcacgcg gcgaaggctc cgcagggccg gcgtcgtgat cgccgccgag aatgcccttc
     7741 accaagttcg acgacacgaa aatcatgctg acggctatca ccatcatgca gacggatcgc
     7801 acgaacccgc agaactcacc cccgaacacg agcacggcac ccgcgaccac tatgccaaga
     7861 atgcccaagg taaaaattgc cggccccgcc atgaagtccg tgaatgcccc gacggccgaa
     7921 gtgaagggca ggccgccacc caggccgccg ccctcactgc ccggcacctg gtcgctgaat
     7981 gtcgatgcca gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc
     8041 catcccgtta ctgccccgat cccggcaatg gcaaggactg ccagcgccgc gatgaggaag
     8101 cgggtgcccc gcttcttcat cttcgcgcct cgggcctcca ggccgcctac ctgggcgaaa
     8161 acatcggtgt ttgtggcatt catacggact cctgttgggc cagctcgcgc acgggctggc
     8221 gggtcagctt ggcttgaaga tcgccacgca ttgcggcgat ctgcttctcg gcatccttgc
     8281 gcttctgcac gccttcctgc tggatgcgaa taacgtcctc gacggtcttg atgagcgtcg
     8341 tctgaacctg cttgagcgtg tccacgtcga tcaccaggcg ttggttctcc ttcgccgttt
     8401 cgacggacgt gcgatgcagc agggccgcat tgcgcttcat caggtcgttg gtggtgtcgt
     8461 cgatggccgt ggccagttcg acggcgttct tctgctcgtt gaggctcaag gccagcatga
     8521 attgccgctt ccacgccggc acggtgattt cgcggatggt gtggaattta tcgaccagca
     8581 tctggttgtt ggcctggatc atgcggatgg tcggcaggct ctgcatggcc gaatgttgca
     8641 aggcgatcag gtcgccgatg cgcttgtcca ggttggcaac catcgcatcg aggtcggcca
     8701 gctcctgcac gcggccaggg tcgttcccga cattgccgcg cagaccctcg gcctgctcgc
     8761 gcagctcggc aaggcggacc ttgccggccg cgatgtggac gccaagaagg cggtgttcct
     8821 cgcgcacggc tgcgaacatt tcgtcgagcg aggcattgcg ctgcgcgatg ccttgctggg
     8881 tggtctgcac ttcgctgacc aggtgttcga tctgctcgcg ggtcgtgtcg aagcgcgcca
     8941 tgaagcccgt cgaacggacg cgggagcggt cgatcagcgg gccaatcagg ggcaggcggg
     9001 aacggttgtc ggacaaaggg ccgacgttca gggaacgggc cttggcgaca acctgggtca
     9061 gtttctcgcc tgcttcgtcc aggtcgctgt tgcgcacctg gtccagcagg ctatcggcgt
     9121 agcgggacgt gtgctcggcc acgtcgcggc cgaactcggc aacggtctgc ggactgccga
     9181 cctcgatccg ctgcgcgacc gcatggactt ccggcacgtc gctttcctgc aagcccagct
     9241 cgcgcagggt tgccggggtc atgtcaaagg cgacgatagg ggccttggcg tcgtgcgtcg
     9301 ttttcagtgc gttcataggg ttctcccgcc gtgttattgg ttgatgcctt ccaggctctg
     9361 cgaaaggctc cgcatgagcg cctggtgagc tttggccgcc tcggcgacca ttgccggatt
     9421 catgttcttg gtggtgatga gcgcgagggt gtgctgacgc cagacgggca ccaggacgga
     9481 tgccgtttca gagaagcggt ccagcatgtc cacggcctgc gcccgcgtga gcttcatctg
     9541 agtgacgctc atttcatggg acgccatgag ggttgccagg ttggcgagct tgcgcgcgaa
     9601 gcgttcgcgc ggcttgtcga actcgatcac gccggccttg gccgcgccgg cctcggggtt
     9661 ctcgtccagg aactcgcgcc cggcttgaat gtaggctctg agccggtcta cctcggcctc
     9721 atgcgtattg agcatgtcat ccaaggcgcg caacgtgtcc cgcacgcgct gcgctacgcc
     9781 ctcggcttcg tccagcaact ggtcgagcgt cttgcgggcg acctgatacc tcacctggcg
     9841 ttcaacctca cggccaagca tcttctcgaa ccaggtaggc ttttccgcga tcttgcgggg
     9901 gtccgcgtcg accagcttcg ccacgatctg gctgattttg tcggccagcg cggcaactgc
     9961 gccgtgctcc atcagattcg acagctcgtt gagggaatcc gccccgtcga tgccggcccc
    10021 gtactcgcca atcgtcgccg gcgacgcgaa gagggcgggc aaaacctccc ccttcaatcg
    10081 cgccatgttc acgcttgttc ttccatggta tatctccttc ttaaagttaa acaaaattat
    10141 tcggaaccca gcatgatatt ccggaaatac caactaagtc aacggctgat atccattgct
    10201 gttgacaaag ggaatcaggg gatcttgaag ttcctattcc gaagttccta ttctctagaa
//
