LOCUS       Exported               11390 bp ds-DNA     circular SYN 24-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 27, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      508..534
                     /regulatory_class="promoter"
                     /label=putative RCR protein gene predicted promoter
                     /note="putative RCR protein gene predicted promoter"
     regulatory      910..930
                     /regulatory_class="terminator"
                     /note="bidirectional Rho-independent transcriptional
                     terminator"
     regulatory      complement(1251..1276)
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     regulatory      1387..1413
                     /regulatory_class="promoter"
                     /label=putative nickase-like gene predicted promoter
                     /note="putative nickase-like gene predicted promoter"
     CDS             1503..1844
                     /codon_start=1
                     /product="putative nickase-like protein"
                     /label=putative nickase-like protein
                     /note="may be involved in rolling circle replication
                     (RCR)"
                     /protein_id="AUJ88099.1"
                     /translation="MKMAIYHCEMQNISRSDGRSIVACAAYRAGEKLYCDTYGKEQDYT
                     KKTGIEYTQIFAPTGASPDMLDRQTLWNRVEQSELKKNGDIKQEARLAKEVEIALPHEL
                     DKTQRQATC"
     terminator      1851..1908
                     /label=his operon terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     rep_origin      complement(2103..2691)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     terminator      complement(2874..2968)
                     /label=lambda t0 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    2991..3975
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1058 selection"
     misc_feature    2991..3093
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1045 selection-2"
     terminator      complement(2993..3079)
                     /label=rrnB T1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    3094..3975
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1053 selection"
     misc_feature    3097..3975
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1020 selection 2-4"
     misc_feature    3097..3971
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from Synthetic NPT III"
     CDS             complement(3097..3891)
                     /codon_start=1
                     /label=KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        3928..3958
                     /note="amiA promoter fragment"
     promoter        3928..3936
                     /label=-10 predicted
     CDS             complement(3987..4646)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(4647..4744)
                     /label=cat promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.1"
                     /note="/other=promoter"
     terminator      complement(4830..4873)
                     /label=bacterial terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    complement(4889..4910)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.6"
                     /note="/other=misc_feature"
     protein_bind    complement(4928..4975)
                     /label=FRT
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     RBS             5067..5089
                     /label=RBS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=RBS"
     CDS             5107..5865
                     /codon_start=1
                     /label=klaA
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=98.4"
                     /note="/other=CDS"
                     /translation="SVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNLMEHG
                     AVAALADKISQIVAKLVDADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQLLDEA
                     EGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEFDKPR
                     ERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLALITT
                     KNMNPAMVAEAAKAHQALMRSLSQSLEGINQ"
     CDS             5886..7019
                     /codon_start=1
                     /label=klaB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE
                     VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN
                     RSRLPLIGPLIDRSRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA
                     VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD
                     LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL
                     ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ
                     EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV"
     CDS             7019..7969
                     /codon_start=1
                     /label=klaC
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV
                     TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF
                     TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP
                     SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG
                     SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG
                     AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP"
     terminator      complement(7990..8021)
                     /label=tonB terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     protein_bind    complement(8085..8118)
                     /label=FRT (minimal)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    8142..8163
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.6"
                     /note="/other=misc_feature"
     CDS             8212..8299
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.1"
                     /note="/other=CDS"
                     /translation="QYANRLSPRVGRFINAAGTTGFPTGKRAV"
     protein_bind    8312..8333
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        8348..8378
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    8382..8404
                     /label=lac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     protein_bind    8386..8402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             8420..8431
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             8438..9115
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      9160..9231
                     /label=rrnB T1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     terminator      9247..9274
                     /label=T7Te terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     regulatory      9315..9344
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     CDS             9468..10028
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88093.1"
                     /translation="MLTRWDNDQSSLSIYHREIKEEQQEQQRKQQQIERETKQKQEDDK
                     KKRQDYERYLSKYGYVKDAEYDHISDHVASDISMFTRLQAQAWASNDMQEYIRCSKQKY
                     EQISDRIAYERDLKKIGQLSRILDKDRQALADILPQLMPYYEQMQQAVNKRKILLENER
                     QPSFKPRYDQEQPKPKKDNDLTF"
     regulatory      10012..10039
                     /regulatory_class="promoter"
                     /label=putative antitoxin-toxin module-predicted promoter
                     /note="putative antitoxin-toxin module-predicted promoter"
     CDS             10073..10366
                     /codon_start=1
                     /product="putative PaaA2-like antitoxin"
                     /label=putative PaaA2-like antitoxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88095.1"
                     /translation="MTEATFTFRVDHDLKQEFSSLAKTVDRSGAQLIRDFMRDFVKKQQ
                     EAADYDKWFKQQVQIGLNEANAGKLIPHEDVKAEFAARRAATLAKLAAKNED"
     CDS             10356..10625
                     /codon_start=1
                     /product="putative ParE2-like toxin"
                     /label=putative ParE2-like toxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88096.1"
                     /translation="MKIEWTETARQDRRNIYDYLEERNPIAAIEIDDLIEEKTDLLVDN
                     RLMGRTGRQKDTRELVIHPHYVVVYDITDIIRILRVLHTSQEWS"
     regulatory      join(10389..11390,1..335)
                     /regulatory_class="replication_regulatory_region"
                     /note="minimal origin of replication for Acinetobacter
                     sp."
     promoter        10574..10582
                     /label=-10 predicted
ORIGIN
        1 aaaaaaaata taattgagat tgtgtcattt cggtcaattc ttaatatgtt ccacgcaagt
       61 tttagctatg gtgctaaaca gaaatttgct gaaaaagaac ttttcactga actggttaaa
      121 atgtaagcag cctgagagcc gccaaaaatt ttaaaaacaa accgccttaa tcatcttcaa
      181 aaaatacctc taaaacctca ccatttgcgt tttaagaccc atatttcatc ctgcccttat
      241 gttcccatgc tgatagctat aaagtgtctg taatcgcttc ctatgacgtt ctaggctgtt
      301 gataactttt ggaacaacgc aaaatgttaa aatccgatca ttttttaacc tagttatttt
      361 cgttacaggg taactttggt agtattattt caatatttag ttgttacagg ataacaaaat
      421 atatgttaca gggtaattaa tttagcgtta caggataact atagttacag ggtaacgtta
      481 aatagttatc ctgtaattta aaaatagttg caggatagca taaattttta caaataaatt
      541 ttataaaaag ttatcctgta actaaaaaca atgttatcct gtaactatga tatactatat
      601 gggagttaaa gacatgaaag acccgaacga tcagaaaaca caggacatgc taaaagagcc
      661 gccaaaaacc aatgccgaac gacaaaaagc atatcgtgaa aaacgcaaaa gccttgatag
      721 tcaacgtttg gaggtgttta tagataaggg tgtgtcggat atgcttgcgg acatggtggg
      781 cgcagcaggg gagagccaaa aagccatttt gaccgcattg attgagaaag agtataagcg
      841 gctgtatgca gtgaaaaata gcaagctaaa aaagtacatt gattcaaaaa gtaatgcaga
      901 taaaaaagaa accctcgatt tatgagggtt ttttataact actaatgcct atgaaaataa
      961 gtgcttatca ttttctctaa tgtacccaat aaaatcgggg ttttgttcca caagtttctt
     1021 gatgtcatta tcatgtttct tcagtaagta agccccgatc aggatcttgc gttttgcatc
     1081 gtctgacctt gcctgttttt gctctcgacc tttcaccaca gcgtcagcta aatcctttct
     1141 tgccttttcc tgacgttcca atttttcctt ttcttcatcg agcatttgac ccaattccat
     1201 agctgttttt ttgaccattt ttaatgcctg tgtctcaacc tattttccga agtatgaacc
     1261 atcatcaatg tgaaaagtgt tctacttcaa aaactcgact tacatcaaga tccgagcgca
     1321 gcgagcaaaa tcaaaaaaca aagtcaaagc cttattgctc ttgcttttgc ttttagcctc
     1381 gcagagttcc cgaagggcgc acttacgcaa aatttttgct acgccaaatt ttgcaagtac
     1441 ggtcagggaa accccgacac cccaaccgcc caaaacttgg ggcggctgtt aataaacagg
     1501 tgatgaaaat ggcaatttac cattgtgaaa tgcagaacat ttcgaggtca gatggtcgct
     1561 caatcgtggc atgtgcagca taccgagcag gcgaaaaatt gtactgtgat acgtacggaa
     1621 aagagcagga ctacaccaaa aaaacaggca ttgaatacac ccaaattttt gccccaactg
     1681 gggcaagtcc tgacatgtta gatcgtcaaa ccctatggaa tcgggtagag caatccgaac
     1741 taaaaaagaa cggtgacatc aaacaggagg caagattagc aaaggaagta gagatagcat
     1801 tgccgcatga actggataag acacagcgtc aggcaacttg ttaactgcag tccggcaaaa
     1861 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
     1921 atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1981 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2041 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2101 tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2161 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2221 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2281 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2341 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2401 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2461 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2521 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2581 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2641 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2701 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2761 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2821 aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca
     2881 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     2941 gtcattactg gatctatcaa caggagtcca agcgagctcg atattctcga ggatttgtcc
     3001 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact
     3061 gagcctttcg ttttatttga tgccgccggc gacgtcctaa aacaattcat ccagtaaaat
     3121 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac
     3181 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca
     3241 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac
     3301 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc
     3361 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt
     3421 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct
     3481 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca
     3541 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca
     3601 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg
     3661 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc
     3721 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt
     3781 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg
     3841 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt
     3901 ccttcctctt ttctacagta tttaaagatt atatcacatt atccattaaa aatcaaacgg
     3961 atccccagct tctcgagaat atcaaattac gccccgccct gccactcatc gcagtactgt
     4021 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     4081 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     4141 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     4201 ggattggctg acacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     4261 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     4321 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     4381 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     4441 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     4501 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     4561 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     4621 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     4681 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     4741 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     4801 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     4861 taaggatgat ttctggaatt cgcggccgcc ccgggtaccg agctcgaatt agcttcaaaa
     4921 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat
     4981 cccctgattc cctttgtcaa cagcaatgga tatcagccgt tgacttagtt ggtatttccg
     5041 gaatatcatg ctgggttccg aataattttg tttaacttta agaaggagat ataccatgga
     5101 agaacaagcg tgaacatggc gcgattgaag ggggaggttt tgcccgccct cttcgcgtcg
     5161 ccggcgacga ttggcgagta cggggccggc atcgacgggg cggattccct caacgagctg
     5221 tcgaatctga tggagcacgg cgcagttgcc gcgctggccg acaaaatcag ccagatcgtg
     5281 gcgaagctgg tcgacgcgga cccccgcaag atcgcggaaa agcctacctg gttcgagaag
     5341 atgcttggcc gtgaggttga acgccaggtg aggtatcagg tcgcccgcaa gacgctcgac
     5401 cagttgctgg acgaagccga gggcgtagcg cagcgcgtgc gggacacgtt gcgcgccttg
     5461 gatgacatgc tcaatacgca tgaggccgag gtagaccggc tcagagccta cattcaagcc
     5521 gggcgcgagt tcctggacga gaaccccgag gccggcgcgg ccaaggccgg cgtgatcgag
     5581 ttcgacaagc cgcgcgaacg cttcgcgcgc aagctcgcca acctggcaac cctcatggcg
     5641 tcccatgaaa tgagcgtcac tcagatgaag ctcacgcggg cgcaggccgt ggacatgctg
     5701 gaccgcttct ctgaaacggc atccgtcctg gtgcccgtct ggcgtcagca caccctcgcg
     5761 ctcatcacca ccaagaacat gaatccggca atggtcgccg aggcggccaa agctcaccag
     5821 gcgctcatgc ggagcctttc gcagagcctg gaaggcatca accaataaca cggcgggaga
     5881 accctatgaa cgcactgaaa acgacgcacg acgccaaggc ccctatcgtc gcctttgaca
     5941 tgaccccggc aaccctgcgc gagctgggct tgcaggaaag cgacgtgccg gaagtccatg
     6001 cggtcgcgca gcggatcgag gtcggcagtc cgcagaccgt tgccgagttc ggccgcgacg
     6061 tggccgagca cacgtcccgc tacgccgata gcctgctgga ccaggtgcgc aacagcgacc
     6121 tggacgaagc aggcgagaaa ctgacccagg ttgtcgccaa ggcccgttcc ctgaacgtcg
     6181 gccctttgtc cgacaaccgt tcccgcctgc ccctgattgg cccgctgatc gaccgctccc
     6241 gcgtccgttc gacgggcttc atggcgcgct tcgacacgac ccgcgagcag atcgaacacc
     6301 tggtcagcga agtgcagacc acccagcaag gcatcgcgca gcgcaatgcc tcgctcgacg
     6361 aaatgttcgc agccgtgcgc gaggaacacc gccttcttgg cgtccacatc gcggccggca
     6421 aggtccgcct tgccgagctg cgcgagcagg ccgagggtct gcgcggcaat gtcgggaacg
     6481 accctggccg cgtgcaggag ctggccgacc tcgatgcgat ggttgccaac ctggacaagc
     6541 gcatcggcga cctgatcgcc ttgcaacatt cggccatgca gagcctgccg accatccgca
     6601 tgatccaggc caacaaccag atgctggtcg ataaattcca caccatccgc gaaatcaccg
     6661 tgccggcgtg gaagcggcaa ttcatgctgg ccttgagcct caacgagcag aagaacgccg
     6721 tcgaactggc cacggccatc gacgacacca ccaacgacct gatgaagcgc aatgcggccc
     6781 tgctgcatcg cacgtccgtc gaaacggcga aggagaacca acgcctggtg atcgacgtgg
     6841 acacgctcaa gcaggttcag acgacgctca tcaagaccgt cgaggacgtt attcgcatcc
     6901 agcaggaagg cgtgcagaag cgcaaggatg ccgagaagca gatcgccgca atgcgtggcg
     6961 atcttcaagc caagctgacc cgccagcccg tgcgcgagct ggcccaacag gagtccgtat
     7021 gaatgccaca aacaccgatg ttttcgccca ggtaggcggc ctggaggccc gaggcgcgaa
     7081 gatgaagaag cggggcaccc gcttcctcat cgcggcgctg gcagtccttg ccattgccgg
     7141 gatcggggca gtaacgggat gggcgatcag cccgagcgcg acgcccggaa gcattgacgt
     7201 gccgcaggtg ctggcatcga cattcagcga ccaggtgccg ggcagtgagg gcggcggcct
     7261 gggtggcggc ctgcccttca cttcggccgt cggggcattc acggacttca tggcggggcc
     7321 ggcaattttt accttgggca ttcttggcat agtggtcgcg ggtgccgtgc tcgtgttcgg
     7381 gggtgagttc tgcgggttcg tgcgatccgt ctgcatgatg gtgatagccg tcagcatgat
     7441 tttcgtgtcg tcgaacttgg tgaagggcat tctcggcggc gatcacgacg ccggccctgc
     7501 ggagccttcg ccgcgtgcgc gattcatggc ggccgtggag gccaaggatt tcgcgcgagt
     7561 gcaagagctg atcgaggcgc gtggagccaa gtcggcggct gattatgtcc ttgcgcagct
     7621 cgccgtggcc gaaggtctgg accgcaagcc tggtgcgcgc gtcgtggtcg ggaaagcggc
     7681 gggcagcatg gcaatgccgc ctgcggcgct gggttttacg ccaaggggag aagcggcata
     7741 cgccatcgag cggtcagcct atggtgagcc gaggtccagc attgcgaagc agtaccagca
     7801 ggaatggaac cggaaggcgg cgacctggtg ggcgatggcc ggtgtggccg gcatcatcgg
     7861 cgcgatcctg gcggcggcgg caaccggctt tgttgggctg gcagtgtcga tccgcaaccg
     7921 agtgaagcgc gtgcgcgacc tgttggtgat ggagccgggt gcagagccat aagggatcga
     7981 tccccctcaa gtcaaaagcc tccggtcgga ggcttttgac tttctgctcg aggagctaat
     8041 tccattgagt aagtttttaa gcacatcagc ttcaaaagcg ctctgaagtt cctatacttt
     8101 ctagagaata ggaacttcgg aataggtact tcaagatccc caattcgagc tcggtacccg
     8161 gggcggccgc ttctagaggt ctcactatat ctagagacgt ctagacatat gcaatacgca
     8221 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     8281 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8341 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8401 aatttcacac atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat
     8461 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
     8521 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
     8581 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
     8641 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
     8701 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
     8761 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
     8821 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
     8881 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
     8941 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
     9001 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
     9061 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
     9121 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
     9181 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     9241 cacactggct caccttcggg tgggcctttc tgcgtttata gcatgccgtc tcattaggta
     9301 cagagaccct gcagttgcaa gacaatatcg accgcttgca gcttgataaa gtgatcaaag
     9361 agggacgagc agctacagca ctgctaaggg agatcggaga gccactgccg ccaccagtga
     9421 aagtgaaaac aggttggttt agtgctgatt attacggctt tgaggacatg ctcacacgtt
     9481 gggataatga tcaatctagc ctttccattt atcacagaga gataaaagag gagcaacagg
     9541 aacagcaacg aaaacaacag cagatagaaa gagaaactaa acaaaaacag gaagatgaca
     9601 aaaagaaacg gcaggactat gaaagatatt tatcaaaata cggctatgtc aaagatgctg
     9661 agtatgacca tatcagcgac catgttgctt cagacatttc catgtttaca cgtcttcagg
     9721 cgcaggcatg ggcaagtaac gatatgcaag agtacataag gtgttcaaag caaaaatacg
     9781 agcagatcag cgacagaata gcctatgagc gtgacttgaa aaaaatcggg caattgagcc
     9841 gtatcctgga taaagacaga caggcacttg ctgatatttt accgcagttg atgccttact
     9901 atgagcagat gcaacaggct gttaataaac ggaaaatttt acttgagaat gagcgacagc
     9961 catcatttaa accacgttat gaccaagaac agcctaagcc gaaaaaagac aatgacctaa
    10021 ctttttaatg tgttacaata gcattcaaat gtaacacatt ggagtgaaaa acatgacaga
    10081 agcgactttt acctttcggg tagaccatga cctaaagcaa gaattttcaa gccttgctaa
    10141 gactgttgat cggtcggggg cgcagctaat ccgtgacttc atgcgtgact ttgttaagaa
    10201 gcaacaggaa gccgcagact atgacaaatg gttcaagcag caggtacaga tcggcttaaa
    10261 tgaagccaat gcaggcaagt tgattccaca tgaggacgta aaggcagagt ttgccgctag
    10321 acgtgctgca acattggcga aactggcagc gaagaatgaa gattgagtgg actgaaacag
    10381 ctcgccaaga tcgtagaaat atctatgatt atcttgaaga acgcaaccct atagcagcta
    10441 ttgaaattga tgatttaatt gaagaaaaga cagatttact tgttgataat cgactgatgg
    10501 ggcgcacagg cagacagaaa gatactaggg agttagtgat acatccgcat tatgtggttg
    10561 tatatgacat cactgatata atacggatac tcagagtgct acacacatcg caggagtggt
    10621 catgacttac tcatgtactt tggattattt agtgttataa aatcctgatt tataaatttt
    10681 ttttgttaaa aaagataaaa gccccttgca attgcttggg gctttaccgt aatttatggg
    10741 gtacagatct tcgatactga catatcggca atcgaaagca ttaaggtttg acgaccgcta
    10801 atgatttcac cacaggggct taatgtacct gtcttaaatt ctaaggtttt aactcgcttt
    10861 gtcaagcata gaccccaaaa atttagccaa tgtctgtaac tcaatctgtc catgtgtggg
    10921 tgatgaggta cagtgacgct agcacacatc ggaaaaacgc tattactagg ggaactgaac
    10981 agagtagcgg acgcaatgag tagtcattta attggcggtt atgagcgtgt tcaggcggtg
    11041 ctatcaatcg taatcataac agtggcagct tgatacagtg atgtcatccc tgatgcgaaa
    11101 gcgaccgacc gacggtacat cgaatgggaa tactttaggg tgatttttaa gaatcgctct
    11161 agggtgagta tttcccattc agctctgctc cctccctctg gtactttaat caaaagcact
    11221 actaaacata tgtttttaaa taaaaaatat tgatatagag ataatattag taagaataat
    11281 taaacaattg aatatagata aatcattgtt aaataaagat taattattaa aatgaatgta
    11341 tacttatata taaatcaatg atttaaaata tttgataaag aaaacttttc
//
