LOCUS       Exported               11285 bp ds-DNA     circular SYN 27-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Merlin Brychcy
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 27, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      339..368
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     primer_bind     complement(349..374)
                     /label=ori_pstI_f
     terminator      375..432
                     /note="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(627..1215)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1398..1492
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    1509..2411
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1058 selection"
     misc_feature    1509..2411
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1061 selection-3"
     misc_feature    1509..2308
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1053 selection"
     misc_feature    1509..2308
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1055 rev selection selection"
     misc_feature    1509..2305
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1020 selection 2-4"
     misc_feature    1509..2305
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from Synthetic NPT III"
     CDS             1511..2305
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-aminoglycoside phosphotransferase type III"
                     /label=Kanamycin Resistance (3',5'-aminoglycoside phos
                     /label=Kanamycin Resistance (3',5'-aminoglycoside 
                     phosphotransferase type III)
                     /note="kanamycin resistance protein; derived from the
                     aphIII gene of plasmid pJH1"
                     /db_xref="GI:33151036"
                     /protein_id="AAP97396.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    2306..2308
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pdl1013 selection rev"
     misc_feature    2306..2308
                     /label=AatII
                     /label=nonstandard type:  Restriction_sit
                     /note="Name: AatII"
                     /note="Pattern: gacgtc"
                     /note="cut_0_on_positive_strand: 5^6"
                     /note="cut_0_on_negative_strand: 1^2"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
     misc_feature    2309..2411
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1045 selection-2"
     misc_feature    2309..2411
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pdl1013 selection rev"
     misc_feature    2309..2411
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1031 selection-2"
     terminator      2323..2409
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2327..2378
                     /label=rrnB T1
     primer_bind     complement(2389..2418)
                     /label=Kan_R_XhoI
     CDS             complement(2425..3084)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      3268..3311
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    3314..3321
                     /label=BioBrick prefix
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ""ATG"""
     primer_bind     3320..3340
                     /label=TelR_NotI_birectional
     protein_bind    complement(3366..3413)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(3366..3385)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    3386..5240
                     /label=tellurite cassette
     protein_bind    complement(3386..3413)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     RBS             3505..3527
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3545..4303
                     /codon_start=1
                     /label=klaA
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=98.4"
                     /note="/other=CDS"
                     /translation="SVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNLMEHG
                     AVAALADKISQIVAKLVDADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQLLDEA
                     EGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEFDKPR
                     ERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLALITT
                     KNMNPAMVAEAAKAHQALMRSLSQSLEGINQ"
     CDS             4324..5457
                     /codon_start=1
                     /label=klaB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE
                     VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN
                     RSRLPLIGPLIDRSRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA
                     VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD
                     LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL
                     ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ
                     EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV"
     primer_bind     complement(5225..5240)
                     /label=pMo130-TelR_pointBsmBI_f
     primer_bind     5241..5260
                     /label=pMO130-TelR_pointBsmBI_r
     CDS             5457..6407
                     /codon_start=1
                     /label=klaC
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV
                     TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF
                     TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP
                     SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG
                     SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG
                     AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP"
     terminator      6428..6459
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    complement(6523..6570)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(6523..6542)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(6588..6607)
                     /label=TelR_NotI_birectional
     misc_feature    6602..6615
                     /label=BioBrick prefix
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ""ATG"""
     primer_bind     complement(6604..6621)
                     /label=2B_first_site_point_r
     misc_feature    6615..6636
                     /label=GG2B
                     /note="GG2B"
     primer_bind     6626..6645
                     /label=2B_to_2D_first_site_f
     primer_bind     6626..6645
                     /label=2B_to_2A_and_2a_first_site_f
     protein_bind    6737..6758
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6773..6803
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6811..6827
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             6863..7540
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /label=monomeric derivative of DsRed (Campbell et al.,
                     /note="mRFP1"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      7585..7656
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      7672..7699
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(7693..7716)
                     /label=2B_point_second_site_r
     misc_feature    7706..7727
                     /label=GG2B
                     /note="GG2B"
     primer_bind     7719..7735
                     /label=2B_to_2A_second_site_f
     primer_bind     7721..7740
                     /label=2B_to_2D_second_site_f
     CDS             complement(7734..8075)
                     /codon_start=1
                     /product="putative nickase-like protein"
                     /label=putative nickase-like protein
                     /note="may be involved in rolling circle replication
                     (RCR)"
                     /protein_id="AUJ88099.1"
                     /translation="MKMAIYHCEMQNISRSDGRSIVACAAYRAGEKLYCDTYGKEQDYT
                     KKTGIEYTQIFAPTGASPDMLDRQTLWNRVEQSELKKNGDIKQEARLAKEVEIALPHEL
                     DKTQRQATC"
     regulatory      8165..8191
                     /regulatory_class="promoter"
                     /label=putative nickase-like gene predicted promoter
                     /note="putative nickase-like gene predicted promoter"
     regulatory      8302..8327
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     CDS             8360..8629
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88098.1"
                     /translation="MVKKTAMELGQMLDEEKEKLERQEKARKDLADAVVKGREQKQARS
                     DDAKRKILIGAYLLKKHDNDIKKLVEQNPDFIGYIRENDKHLFS"
     regulatory      8648..8668
                     /regulatory_class="terminator"
                     /note="bidirectional Rho-independent transcriptional
                     terminator"
     regulatory      9044..9070
                     /regulatory_class="promoter"
                     /label=putative RCR protein gene predicted promoter
                     /note="putative RCR protein gene predicted promoter"
     regulatory      9243..10579
                     /regulatory_class="replication_regulatory_region"
                     /note="minimal origin of replication for Acinetobacter
                     sp."
     CDS             complement(10343..10612)
                     /codon_start=1
                     /product="putative ParE2-like toxin"
                     /label=putative ParE2-like toxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88096.1"
                     /translation="MKIEWTETARQDRRNIYDYLEERNPIAAIEIDDLIEEKTDLLVDN
                     RLMGRTGRQKDTRELVIHPHYVVVYDITDIIRILRVLHTSQEWS"
     CDS             complement(10602..10895)
                     /codon_start=1
                     /product="putative PaaA2-like antitoxin"
                     /label=putative PaaA2-like antitoxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88095.1"
                     /translation="MTEATFTFRVDHDLKQEFSSLAKTVDRSGAQLIRDFMRDFVKKQQ
                     EAADYDKWFKQQVQIGLNEANAGKLIPHEDVKAEFAARRAATLAKLAAKNED"
     regulatory      10929..10956
                     /regulatory_class="promoter"
                     /label=putative antitoxin-toxin module-predicted promoter
                     /note="putative antitoxin-toxin module-predicted promoter"
     CDS             complement(join(10940..11285,1..215))
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88093.1"
                     /translation="MLTRWDNDQSSLSIYHREIKEEQQEQQRKQQQIERETKQKQEDDK
                     KKRQDYERYLSKYGYVKDAEYDHISDHVASDISMFTRLQAQAWASNDMQEYIRCSKQKY
                     EQISDRIAYERDLKKIGQLSRILDKDRQALADILPQLMPYYEQMQQAVNKRKILLENER
                     QPSFKPRYDQEQPKPKKDNDLTF"
ORIGIN
        1 tggtcgctga tatggtcata ctcagcatct ttgacatagc cgtattttga taaatatctt
       61 tcatagtcct gccgtttctt tttgtcatct tcctgttttt gtttagtttc tctttctatc
      121 tgctgttgtt ttcgttgctg ttcctgttgc tcctctttta tctctctgtg ataaatggaa
      181 aggctagatt gatcattatc ccaacgtgtg agcatgtcct caaagccgta ataatcagca
      241 ctaaaccaac ctgttttcac tttcactggt ggcggcagtg gctctccgat ctcccttagc
      301 agtgctgtag ctgctcgtcc ctctttgatc actttatcaa gctgcaagcg gtcgatattg
      361 tcttgcaact gcagtccggc aaaaaagggc aaggtgtcac caccctgccc tttttcttta
      421 aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg
      481 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      541 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      601 cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac
      661 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      721 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      781 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      841 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      901 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      961 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1021 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1081 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1141 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1201 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1261 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1321 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1381 gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     1441 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     1501 ctcgatataa atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata
     1561 ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga
     1621 aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt
     1681 ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct
     1741 gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct
     1801 ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga
     1861 gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga
     1921 cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga
     1981 aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac
     2041 ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt
     2101 tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa
     2161 gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta
     2221 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta
     2281 tattttactg gatgaattgt tttaggacgt cgccggcggc atcaaataaa acgaaaggct
     2341 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     2401 aggacaaatc ctcgagaaat caaattacgc cccgccctgc cactcatcgc agtactgttg
     2461 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     2521 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     2581 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     2641 attggctgac acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     2701 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     2761 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     2821 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     2881 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     2941 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     3001 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     3061 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     3121 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     3181 cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     3241 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
     3301 aggatgattt ctggaattcg cggccgcccc gggtaccgag ctcgaattag cttcaaaagc
     3361 gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac ttcaagatcc
     3421 cctgattccc tttgtcaaca gcaatggata tcagccgttg acttagttgg tatttccgga
     3481 atatcatgct gggttccgaa taattttgtt taactttaag aaggagatat accatggaag
     3541 aacaagcgtg aacatggcgc gattgaaggg ggaggttttg cccgccctct tcgcgtcgcc
     3601 ggcgacgatt ggcgagtacg gggccggcat cgacggggcg gattccctca acgagctgtc
     3661 gaatctgatg gagcacggcg cagttgccgc gctggccgac aaaatcagcc agatcgtggc
     3721 gaagctggtc gacgcggacc cccgcaagat cgcggaaaag cctacctggt tcgagaagat
     3781 gcttggccgt gaggttgaac gccaggtgag gtatcaggtc gcccgcaaga cgctcgacca
     3841 gttgctggac gaagccgagg gcgtagcgca gcgcgtgcgg gacacgttgc gcgccttgga
     3901 tgacatgctc aatacgcatg aggccgaggt agaccggctc agagcctaca ttcaagccgg
     3961 gcgcgagttc ctggacgaga accccgaggc cggcgcggcc aaggccggcg tgatcgagtt
     4021 cgacaagccg cgcgaacgct tcgcgcgcaa gctcgccaac ctggcaaccc tcatggcgtc
     4081 ccatgaaatg agcgtcactc agatgaagct cacgcgggcg caggccgtgg acatgctgga
     4141 ccgcttctct gaaacggcat ccgtcctggt gcccgtctgg cgtcagcaca ccctcgcgct
     4201 catcaccacc aagaacatga atccggcaat ggtcgccgag gcggccaaag ctcaccaggc
     4261 gctcatgcgg agcctttcgc agagcctgga aggcatcaac caataacacg gcgggagaac
     4321 cctatgaacg cactgaaaac gacgcacgac gccaaggccc ctatcgtcgc ctttgacatg
     4381 accccggcaa ccctgcgcga gctgggcttg caggaaagcg acgtgccgga agtccatgcg
     4441 gtcgcgcagc ggatcgaggt cggcagtccg cagaccgttg ccgagttcgg ccgcgacgtg
     4501 gccgagcaca cgtcccgcta cgccgatagc ctgctggacc aggtgcgcaa cagcgacctg
     4561 gacgaagcag gcgagaaact gacccaggtt gtcgccaagg cccgttccct gaacgtcggc
     4621 cctttgtccg acaaccgttc ccgcctgccc ctgattggcc cgctgatcga ccgctcccgc
     4681 gtccgttcga cgggcttcat ggcgcgcttc gacacgaccc gcgagcagat cgaacacctg
     4741 gtcagcgaag tgcagaccac ccagcaaggc atcgcgcagc gcaatgcctc gctcgacgaa
     4801 atgttcgcag ccgtgcgcga ggaacaccgc cttcttggcg tccacatcgc ggccggcaag
     4861 gtccgccttg ccgagctgcg cgagcaggcc gagggtctgc gcggcaatgt cgggaacgac
     4921 cctggccgcg tgcaggagct ggccgacctc gatgcgatgg ttgccaacct ggacaagcgc
     4981 atcggcgacc tgatcgcctt gcaacattcg gccatgcaga gcctgccgac catccgcatg
     5041 atccaggcca acaaccagat gctggtcgat aaattccaca ccatccgcga aatcaccgtg
     5101 ccggcgtgga agcggcaatt catgctggcc ttgagcctca acgagcagaa gaacgccgtc
     5161 gaactggcca cggccatcga cgacaccacc aacgacctga tgaagcgcaa tgcggccctg
     5221 ctgcatcgca cgtccgtcga aacggcgaag gagaaccaac gcctggtgat cgacgtggac
     5281 acgctcaagc aggttcagac gacgctcatc aagaccgtcg aggacgttat tcgcatccag
     5341 caggaaggcg tgcagaagcg caaggatgcc gagaagcaga tcgccgcaat gcgtggcgat
     5401 cttcaagcca agctgacccg ccagcccgtg cgcgagctgg cccaacagga gtccgtatga
     5461 atgccacaaa caccgatgtt ttcgcccagg taggcggcct ggaggcccga ggcgcgaaga
     5521 tgaagaagcg gggcacccgc ttcctcatcg cggcgctggc agtccttgcc attgccggga
     5581 tcggggcagt aacgggatgg gcgatcagcc cgagcgcgac gcccggaagc attgacgtgc
     5641 cgcaggtgct ggcatcgaca ttcagcgacc aggtgccggg cagtgagggc ggcggcctgg
     5701 gtggcggcct gcccttcact tcggccgtcg gggcattcac ggacttcatg gcggggccgg
     5761 caatttttac cttgggcatt cttggcatag tggtcgcggg tgccgtgctc gtgttcgggg
     5821 gtgagttctg cgggttcgtg cgatccgtct gcatgatggt gatagccgtc agcatgattt
     5881 tcgtgtcgtc gaacttggtg aagggcattc tcggcggcga tcacgacgcc ggccctgcgg
     5941 agccttcgcc gcgtgcgcga ttcatggcgg ccgtggaggc caaggatttc gcgcgagtgc
     6001 aagagctgat cgaggcgcgt ggagccaagt cggcggctga ttatgtcctt gcgcagctcg
     6061 ccgtggccga aggtctggac cgcaagcctg gtgcgcgcgt cgtggtcggg aaagcggcgg
     6121 gcagcatggc aatgccgcct gcggcgctgg gttttacgcc aaggggagaa gcggcatacg
     6181 ccatcgagcg gtcagcctat ggtgagccga ggtccagcat tgcgaagcag taccagcagg
     6241 aatggaaccg gaaggcggcg acctggtggg cgatggccgg tgtggccggc atcatcggcg
     6301 cgatcctggc ggcggcggca accggctttg ttgggctggc agtgtcgatc cgcaaccgag
     6361 tgaagcgcgt gcgcgacctg ttggtgatgg agccgggtgc agagccataa gggatcgatc
     6421 cccctcaagt caaaagcctc cggtcggagg cttttgactt tctgctcgag gagctaattc
     6481 cattgagtaa gtttttaagc acatcagctt caaaagcgct ctgaagttcc tatactttct
     6541 agagaatagg aacttcggaa taggtacttc aagatcccca attcgagctc ggtacccggg
     6601 cggccgcttc tagaggtctc agtacatcta gagacgcaat acgcaaaccg cctctccccg
     6661 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6721 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6781 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact
     6841 agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag agttcatgcg
     6901 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
     6961 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
     7021 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
     7081 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
     7141 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
     7201 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
     7261 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
     7321 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
     7381 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
     7441 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
     7501 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg
     7561 tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     7621 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     7681 tcgggtgggc ctttctgcgt ttatacgtct cattagggac agagaccctg cagttaacaa
     7741 gttgcctgac gctgtgtctt atccagttca tgcggcaatg ctatctctac ttcctttgct
     7801 aatcttgcct cctgtttgat gtcaccgttc ttttttagtt cggattgctc tacccgattc
     7861 catagggttt gacgatctaa catgtcagga cttgccccag ttggggcaaa aatttgggtg
     7921 tattcaatgc ctgttttttt ggtgtagtcc tgctcttttc cgtacgtatc acagtacaat
     7981 ttttcgcctg ctcggtatgc tgcacatgcc acgattgagc gaccatctga cctcgaaatg
     8041 ttctgcattt cacaatggta aattgccatt ttcatcacct gtttattaac agccgcccca
     8101 agttttgggc ggttggggtg tcggggtttc cctgaccgta cttgcaaaat ttggcgtagc
     8161 aaaaattttg cgtaagtgcg cccttcggga actctgcgag gctaaaagca aaagcaagag
     8221 caataaggct ttgactttgt tttttgattt tgctcgctgc gctcggatct tgatgtaagt
     8281 cgagtttttg aagtagaaca cttttcacat tgatgatggt tcatacttcg gaaaataggt
     8341 tgagacacag gcattaaaaa tggtcaaaaa aacagctatg gaattgggtc aaatgctcga
     8401 tgaagaaaag gaaaaattgg aacgtcagga aaaggcaaga aaggatctag ctgacgctgt
     8461 ggtgaaaggt cgagagcaaa aacaggcaag gtcagacgat gcaaaacgca agatcctgat
     8521 cggggcttac ttactgaaga aacatgataa tgacatcaag aaacttgtgg aacaaaaccc
     8581 cgattttatt gggtacatta gagaaaatga taagcactta ttttcatagg cattagtagt
     8641 tataaaaaac cctcataaat cgagggtttc ttttttatct gcattacttt ttgaatcaat
     8701 gtactttttt agcttgctat ttttcactgc atacagccgc ttatactctt tctcaatcaa
     8761 tgcggtcaaa atggcttttt ggctctcccc tgctgcgccc accatgtccg caagcatatc
     8821 cgacacaccc ttatctataa acacctccaa acgttgacta tcaaggcttt tgcgtttttc
     8881 acgatatgct ttttgtcgtt cggcattggt ttttggcggc tcttttagca tgtcctgtgt
     8941 tttctgatcg ttcgggtctt tcatgtcttt aactcccata tagtatatca tagttacagg
     9001 ataacattgt ttttagttac aggataactt tttataaaat ttatttgtaa aaatttatgc
     9061 tatcctgcaa ctatttttaa attacaggat aactatttaa cgttaccctg taactatagt
     9121 tatcctgtaa cgctaaatta attaccctgt aacatatatt ttgttatcct gtaacaacta
     9181 aatattgaaa taatactacc aaagttaccc tgtaacgaaa ataactaggt taaaaaatga
     9241 tcggatttta acattttgcg ttgttccaaa agttatcaac agcctagaac gtcataggaa
     9301 gcgattacag acactttata gctatcagca tgggaacata agggcaggat gaaatatggg
     9361 tcttaaaacg caaatggtga ggttttagag gtattttttg aagatgatta aggcggtttg
     9421 tttttaaaat ttttggcggc tctcaggctg cttacatttt aaccagttca gtgaaaagtt
     9481 ctttttcagc aaatttctgt ttagcaccat agctaaaact tgcgtggaac atattaagaa
     9541 ttgaccgaaa tgacacaatc tcaattatat tttttttgaa aagttttctt tatcaaatat
     9601 tttaaatcat tgatttatat ataagtatac attcatttta ataattaatc tttatttaac
     9661 aatgatttat ctatattcaa ttgtttaatt attcttacta atattatctc tatatcaata
     9721 ttttttattt aaaaacatat gtttagtagt gcttttgatt aaagtaccag agggagggag
     9781 cagagctgaa tgggaaatac tcaccctaga gcgattctta aaaatcaccc taaagtattc
     9841 ccattcgatg taccgtcggt cggtcgcttt cgcatcaggg atgacatcac tgtatcaagc
     9901 tgccactgtt atgattacga ttgatagcac cgcctgaaca cgctcataac cgccaattaa
     9961 atgactactc attgcgtccg ctactctgtt cagttcccct agtaatagcg tttttccgat
    10021 gtgtgctagc gtcactgtac ctcatcaccc acacatggac agattgagtt acagacattg
    10081 gctaaatttt tggggtctat gcttgacaaa gcgagttaaa accttagaat ttaagacagg
    10141 tacattaagc ccctgtggtg aaatcattag cggtcgtcaa accttaatgc tttcgattgc
    10201 cgatatgtca gtatcgaaga tctgtacccc ataaattacg gtaaagcccc aagcaattgc
    10261 aaggggcttt tatctttttt aacaaaaaaa atttataaat caggatttta taacactaaa
    10321 taatccaaag tacatgagta agtcatgacc actcctgcga tgtgtgtagc actctgagta
    10381 tccgtattat atcagtgatg tcatatacaa ccacataatg cggatgtatc actaactccc
    10441 tagtatcttt ctgtctgcct gtgcgcccca tcagtcgatt atcaacaagt aaatctgtct
    10501 tttcttcaat taaatcatca atttcaatag ctgctatagg gttgcgttct tcaagataat
    10561 catagatatt tctacgatct tggcgagctg tttcagtcca ctcaatcttc attcttcgct
    10621 gccagtttcg ccaatgttgc agcacgtcta gcggcaaact ctgcctttac gtcctcatgt
    10681 ggaatcaact tgcctgcatt ggcttcattt aagccgatct gtacctgctg cttgaaccat
    10741 ttgtcatagt ctgcggcttc ctgttgcttc ttaacaaagt cacgcatgaa gtcacggatt
    10801 agctgcgccc ccgaccgatc aacagtctta gcaaggcttg aaaattcttg ctttaggtca
    10861 tggtctaccc gaaaggtaaa agtcgcttct gtcatgtttt tcactccaat gtgttacatt
    10921 tgaatgctat tgtaacacat taaaaagtta ggtcattgtc ttttttcggc ttaggctgtt
    10981 cttggtcata acgtggttta aatgatggct gtcgctcatt ctcaagtaaa attttccgtt
    11041 tattaacagc ctgttgcatc tgctcatagt aaggcatcaa ctgcggtaaa atatcagcaa
    11101 gtgcctgtct gtctttatcc aggatacggc tcaattgccc gatttttttc aagtcacgct
    11161 cataggctat tctgtcgctg atctgctcgt atttttgctt tgaacacctt atgtactctt
    11221 gcatatcgtt acttgcccat gcctgcgcct gaagacgtgt aaacatggaa atgtctgaag
    11281 caaca
//
