LOCUS       Exported               11271 bp ds-DNA     circular SYN 27-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11271)
  AUTHORS   Merlin Brychcy
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 27, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11271
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..22
                     /label=GG2D
                     /note="GG2D"
     protein_bind    123..144
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        159..189
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    197..213
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             249..926
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /label=monomeric derivative of DsRed
                     /note="mRFP1"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      971..1042
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1058..1085
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    1092..1113
                     /label=GG2D
                     /note="GG2D"
     primer_bind     1114..1139
                     /label=ori_pstI_f
     regulatory      1120..1149
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     CDS             1273..1833
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88093.1"
                     /translation="MLTRWDNDQSSLSIYHREIKEEQQEQQRKQQQIERETKQKQEDDK
                     KKRQDYERYLSKYGYVKDAEYDHISDHVASDISMFTRLQAQAWASNDMQEYIRCSKQKY
                     EQISDRIAYERDLKKIGQLSRILDKDRQALADILPQLMPYYEQMQQAVNKRKILLENER
                     QPSFKPRYDQEQPKPKKDNDLTF"
     regulatory      1817..1844
                     /regulatory_class="promoter"
                     /label=putative antitoxin-toxin module-predicted promoter
                     /note="putative antitoxin-toxin module-predicted promoter"
     CDS             1878..2171
                     /codon_start=1
                     /product="putative PaaA2-like antitoxin"
                     /label=putative PaaA2-like antitoxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88095.1"
                     /translation="MTEATFTFRVDHDLKQEFSSLAKTVDRSGAQLIRDFMRDFVKKQQ
                     EAADYDKWFKQQVQIGLNEANAGKLIPHEDVKAEFAARRAATLAKLAAKNED"
     CDS             2161..2430
                     /codon_start=1
                     /product="putative ParE2-like toxin"
                     /label=putative ParE2-like toxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88096.1"
                     /translation="MKIEWTETARQDRRNIYDYLEERNPIAAIEIDDLIEEKTDLLVDN
                     RLMGRTGRQKDTRELVIHPHYVVVYDITDIIRILRVLHTSQEWS"
     regulatory      2194..3530
                     /regulatory_class="replication_regulatory_region"
                     /note="minimal origin of replication for Acinetobacter
                     sp."
     regulatory      3703..3729
                     /regulatory_class="promoter"
                     /label=putative RCR protein gene predicted promoter
                     /note="putative RCR protein gene predicted promoter"
     regulatory      4105..4125
                     /regulatory_class="terminator"
                     /note="bidirectional Rho-independent transcriptional
                     terminator"
     CDS             complement(4144..4413)
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88098.1"
                     /translation="MVKKTAMELGQMLDEEKEKLERQEKARKDLADAVVKGREQKQARS
                     DDAKRKILIGAYLLKKHDNDIKKLVEQNPDFIGYIRENDKHLFS"
     regulatory      complement(4446..4471)
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     regulatory      4582..4608
                     /regulatory_class="promoter"
                     /label=putative nickase-like gene predicted promoter
                     /note="putative nickase-like gene predicted promoter"
     CDS             4698..5039
                     /codon_start=1
                     /product="putative nickase-like protein"
                     /label=putative nickase-like protein
                     /note="may be involved in rolling circle replication
                     (RCR)"
                     /protein_id="AUJ88099.1"
                     /translation="MKMAIYHCEMQNISRSDGRSIVACAAYRAGEKLYCDTYGKEQDYT
                     KKTGIEYTQIFAPTGASPDMLDRQTLWNRVEQSELKKNGDIKQEARLAKEVEIALPHEL
                     DKTQRQATC"
     terminator      5046..5103
                     /note="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(5298..5886)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      6069..6163
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     primer_bind     6119..6138
                     /label=2D_sequencing
     misc_feature    6178..7070
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1058 selection"
     misc_feature    6178..7070
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1061 selection-3"
     misc_feature    6178..6188
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1045 selection-2"
     misc_feature    6178..6188
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pdl1013 selection rev"
     misc_feature    6178..6188
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1031 selection-2"
     misc_feature    6189..7070
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1053 selection"
     misc_feature    6189..7070
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1055 rev selection selection"
     misc_feature    6189..6191
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pdl1013 selection rev"
     misc_feature    6189..6191
                     /label=AatII
                     /label=nonstandard type:  Restriction_sit
                     /note="Name: AatII"
                     /note="Pattern: gacgtc"
                     /note="cut_0_on_positive_strand: 5^6"
                     /note="cut_0_on_negative_strand: 1^2"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
     misc_feature    6192..7070
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1020 selection 2-4"
     misc_feature    6192..7066
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from Synthetic NPT III"
     CDS             complement(6192..6986)
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-aminoglycoside phosphotransferase type III"
                     /label=Kanamycin Resistance (3',5'-aminoglycoside phos
                     /label=Kanamycin Resistance (3',5'-aminoglycoside 
                     phosphotransferase type III)
                     /note="kanamycin resistance protein; derived from the
                     aphIII gene of plasmid pJH1"
                     /db_xref="GI:33151036"
                     /protein_id="AAP97396.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     RBS             6999..7003
     promoter        7023..7053
                     /note="amiA promoter fragment"
     promoter        7023..7031
                     /label=-10 predicted
     promoter        7046..7051
                     /label=-35 predicted
     primer_bind     complement(7052..7074)
                     /label=Kan_F_XhoI
     misc_feature    7067..7070
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pEMKan selection selection"
     CDS             complement(7081..7740)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      7924..7967
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    7970..7977
                     /label=BioBrick prefix
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ""ATG"""
     primer_bind     7976..7996
                     /label=TelR_NotI_birectional
     protein_bind    complement(8022..8069)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(8022..8041)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    8042..9896
                     /label=tellurite cassette
     protein_bind    complement(8042..8069)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     RBS             8161..8183
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             8201..8959
                     /codon_start=1
                     /label=klaA
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=98.4"
                     /note="/other=CDS"
                     /translation="SVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNLMEHG
                     AVAALADKISQIVAKLVDADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQLLDEA
                     EGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEFDKPR
                     ERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLALITT
                     KNMNPAMVAEAAKAHQALMRSLSQSLEGINQ"
     CDS             8980..10113
                     /codon_start=1
                     /label=klaB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE
                     VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN
                     RSRLPLIGPLIDRSRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA
                     VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD
                     LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL
                     ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ
                     EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV"
     primer_bind     complement(9881..9896)
                     /label=pMo130-TelR_pointBsmBI_f
     primer_bind     9897..9916
                     /label=pMO130-TelR_pointBsmBI_r
     CDS             10113..11063
                     /codon_start=1
                     /label=klaC
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV
                     TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF
                     TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP
                     SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG
                     SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG
                     AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP"
     terminator      11084..11115
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    complement(11179..11226)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(11179..11198)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(11244..11264)
                     /label=TelR_NotI_birectional
     misc_feature    join(11259..11271,1)
                     /label=BioBrick prefix
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ""ATG"""
ORIGIN
        1 ggtctcatcg aatctagaga cgcaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      181 cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga
      241 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
      301 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
      361 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
      421 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
      481 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
      541 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
      601 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
      661 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
      721 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
      781 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
      841 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
      901 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
      961 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1021 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1081 ctgcgtttat acgtctcatt agtgttagag accctgcagt tgcaagacaa tatcgaccgc
     1141 ttgcagcttg ataaagtgat caaagaggga cgagcagcta cagcactgct aagggagatc
     1201 ggagagccac tgccgccacc agtgaaagtg aaaacaggtt ggtttagtgc tgattattac
     1261 ggctttgagg acatgctcac acgttgggat aatgatcaat ctagcctttc catttatcac
     1321 agagagataa aagaggagca acaggaacag caacgaaaac aacagcagat agaaagagaa
     1381 actaaacaaa aacaggaaga tgacaaaaag aaacggcagg actatgaaag atatttatca
     1441 aaatacggct atgtcaaaga tgctgagtat gaccatatca gcgaccatgt tgcttcagac
     1501 atttccatgt ttacacgtct tcaggcgcag gcatgggcaa gtaacgatat gcaagagtac
     1561 ataaggtgtt caaagcaaaa atacgagcag atcagcgaca gaatagccta tgagcgtgac
     1621 ttgaaaaaaa tcgggcaatt gagccgtatc ctggataaag acagacaggc acttgctgat
     1681 attttaccgc agttgatgcc ttactatgag cagatgcaac aggctgttaa taaacggaaa
     1741 attttacttg agaatgagcg acagccatca tttaaaccac gttatgacca agaacagcct
     1801 aagccgaaaa aagacaatga cctaactttt taatgtgtta caatagcatt caaatgtaac
     1861 acattggagt gaaaaacatg acagaagcga cttttacctt tcgggtagac catgacctaa
     1921 agcaagaatt ttcaagcctt gctaagactg ttgatcggtc gggggcgcag ctaatccgtg
     1981 acttcatgcg tgactttgtt aagaagcaac aggaagccgc agactatgac aaatggttca
     2041 agcagcaggt acagatcggc ttaaatgaag ccaatgcagg caagttgatt ccacatgagg
     2101 acgtaaaggc agagtttgcc gctagacgtg ctgcaacatt ggcgaaactg gcagcgaaga
     2161 atgaagattg agtggactga aacagctcgc caagatcgta gaaatatcta tgattatctt
     2221 gaagaacgca accctatagc agctattgaa attgatgatt taattgaaga aaagacagat
     2281 ttacttgttg ataatcgact gatggggcgc acaggcagac agaaagatac tagggagtta
     2341 gtgatacatc cgcattatgt ggttgtatat gacatcactg atataatacg gatactcaga
     2401 gtgctacaca catcgcagga gtggtcatga cttactcatg tactttggat tatttagtgt
     2461 tataaaatcc tgatttataa attttttttg ttaaaaaaga taaaagcccc ttgcaattgc
     2521 ttggggcttt accgtaattt atggggtaca gatcttcgat actgacatat cggcaatcga
     2581 aagcattaag gtttgacgac cgctaatgat ttcaccacag gggcttaatg tacctgtctt
     2641 aaattctaag gttttaactc gctttgtcaa gcatagaccc caaaaattta gccaatgtct
     2701 gtaactcaat ctgtccatgt gtgggtgatg aggtacagtg acgctagcac acatcggaaa
     2761 aacgctatta ctaggggaac tgaacagagt agcggacgca atgagtagtc atttaattgg
     2821 cggttatgag cgtgttcagg cggtgctatc aatcgtaatc ataacagtgg cagcttgata
     2881 cagtgatgtc atccctgatg cgaaagcgac cgaccgacgg tacatcgaat gggaatactt
     2941 tagggtgatt tttaagaatc gctctagggt gagtatttcc cattcagctc tgctccctcc
     3001 ctctggtact ttaatcaaaa gcactactaa acatatgttt ttaaataaaa aatattgata
     3061 tagagataat attagtaaga ataattaaac aattgaatat agataaatca ttgttaaata
     3121 aagattaatt attaaaatga atgtatactt atatataaat caatgattta aaatatttga
     3181 taaagaaaac ttttcaaaaa aaatataatt gagattgtgt catttcggtc aattcttaat
     3241 atgttccacg caagttttag ctatggtgct aaacagaaat ttgctgaaaa agaacttttc
     3301 actgaactgg ttaaaatgta agcagcctga gagccgccaa aaattttaaa aacaaaccgc
     3361 cttaatcatc ttcaaaaaat acctctaaaa cctcaccatt tgcgttttaa gacccatatt
     3421 tcatcctgcc cttatgttcc catgctgata gctataaagt gtctgtaatc gcttcctatg
     3481 acgttctagg ctgttgataa cttttggaac aacgcaaaat gttaaaatcc gatcattttt
     3541 taacctagtt attttcgtta cagggtaact ttggtagtat tatttcaata tttagttgtt
     3601 acaggataac aaaatatatg ttacagggta attaatttag cgttacagga taactatagt
     3661 tacagggtaa cgttaaatag ttatcctgta atttaaaaat agttgcagga tagcataaat
     3721 ttttacaaat aaattttata aaaagttatc ctgtaactaa aaacaatgtt atcctgtaac
     3781 tatgatatac tatatgggag ttaaagacat gaaagacccg aacgatcaga aaacacagga
     3841 catgctaaaa gagccgccaa aaaccaatgc cgaacgacaa aaagcatatc gtgaaaaacg
     3901 caaaagcctt gatagtcaac gtttggaggt gtttatagat aagggtgtgt cggatatgct
     3961 tgcggacatg gtgggcgcag caggggagag ccaaaaagcc attttgaccg cattgattga
     4021 gaaagagtat aagcggctgt atgcagtgaa aaatagcaag ctaaaaaagt acattgattc
     4081 aaaaagtaat gcagataaaa aagaaaccct cgatttatga gggtttttta taactactaa
     4141 tgcctatgaa aataagtgct tatcattttc tctaatgtac ccaataaaat cggggttttg
     4201 ttccacaagt ttcttgatgt cattatcatg tttcttcagt aagtaagccc cgatcaggat
     4261 cttgcgtttt gcatcgtctg accttgcctg tttttgctct cgacctttca ccacagcgtc
     4321 agctagatcc tttcttgcct tttcctgacg ttccaatttt tccttttctt catcgagcat
     4381 ttgacccaat tccatagctg tttttttgac catttttaat gcctgtgtct caacctattt
     4441 tccgaagtat gaaccatcat caatgtgaaa agtgttctac ttcaaaaact cgacttacat
     4501 caagatccga gcgcagcgag caaaatcaaa aaacaaagtc aaagccttat tgctcttgct
     4561 tttgctttta gcctcgcaga gttcccgaag ggcgcactta cgcaaaattt ttgctacgcc
     4621 aaattttgca agtacggtca gggaaacccc gacaccccaa ccgcccaaaa cttggggcgg
     4681 ctgttaataa acaggtgatg aaaatggcaa tttaccattg tgaaatgcag aacatttcga
     4741 ggtcagatgg tcgctcaatc gtggcatgtg cagcataccg agcaggcgaa aaattgtact
     4801 gtgatacgta cggaaaagag caggactaca ccaaaaaaac aggcattgaa tacacccaaa
     4861 tttttgcccc aactggggca agtcctgaca tgttagatcg tcaaacccta tggaatcggg
     4921 tagagcaatc cgaactaaaa aagaacggtg acatcaaaca ggaggcaaga ttagcaaagg
     4981 aagtagagat agcattgccg catgaactgg ataagacaca gcgtcaggca acttgttaac
     5041 tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
     5101 agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5161 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5221 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5281 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
     5341 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5401 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5461 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5521 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5581 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5641 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5701 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5761 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5821 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5881 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5941 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6001 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg
     6061 aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     6121 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatccg
     6181 ccggcgacgt cctaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca
     6241 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc
     6301 ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca
     6361 agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg
     6421 ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg
     6481 cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg
     6541 ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa
     6601 tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt
     6661 tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg
     6721 agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt
     6781 ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac
     6841 cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta
     6901 gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat
     6961 tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa
     7021 agattatatc acattatcca ttaaaaatca aacggatccc cagcttctcg agaaatcaaa
     7081 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     7141 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     7201 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     7261 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgacacga aaaacatatt
     7321 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     7381 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     7441 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     7501 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     7561 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     7621 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     7681 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     7741 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     7801 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
     7861 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     7921 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
     7981 cgccccgggt accgagctcg aattagcttc aaaagcgctc tgaagttcct atactttcta
     8041 gagaatagga acttcggaat aggaacttca agatcccctg attccctttg tcaacagcaa
     8101 tggatatcag ccgttgactt agttggtatt tccggaatat catgctgggt tccgaataat
     8161 tttgtttaac tttaagaagg agatatacca tggaagaaca agcgtgaaca tggcgcgatt
     8221 gaagggggag gttttgcccg ccctcttcgc gtcgccggcg acgattggcg agtacggggc
     8281 cggcatcgac ggggcggatt ccctcaacga gctgtcgaat ctgatggagc acggcgcagt
     8341 tgccgcgctg gccgacaaaa tcagccagat cgtggcgaag ctggtcgacg cggacccccg
     8401 caagatcgcg gaaaagccta cctggttcga gaagatgctt ggccgtgagg ttgaacgcca
     8461 ggtgaggtat caggtcgccc gcaagacgct cgaccagttg ctggacgaag ccgagggcgt
     8521 agcgcagcgc gtgcgggaca cgttgcgcgc cttggatgac atgctcaata cgcatgaggc
     8581 cgaggtagac cggctcagag cctacattca agccgggcgc gagttcctgg acgagaaccc
     8641 cgaggccggc gcggccaagg ccggcgtgat cgagttcgac aagccgcgcg aacgcttcgc
     8701 gcgcaagctc gccaacctgg caaccctcat ggcgtcccat gaaatgagcg tcactcagat
     8761 gaagctcacg cgggcgcagg ccgtggacat gctggaccgc ttctctgaaa cggcatccgt
     8821 cctggtgccc gtctggcgtc agcacaccct cgcgctcatc accaccaaga acatgaatcc
     8881 ggcaatggtc gccgaggcgg ccaaagctca ccaggcgctc atgcggagcc tttcgcagag
     8941 cctggaaggc atcaaccaat aacacggcgg gagaacccta tgaacgcact gaaaacgacg
     9001 cacgacgcca aggcccctat cgtcgccttt gacatgaccc cggcaaccct gcgcgagctg
     9061 ggcttgcagg aaagcgacgt gccggaagtc catgcggtcg cgcagcggat cgaggtcggc
     9121 agtccgcaga ccgttgccga gttcggccgc gacgtggccg agcacacgtc ccgctacgcc
     9181 gatagcctgc tggaccaggt gcgcaacagc gacctggacg aagcaggcga gaaactgacc
     9241 caggttgtcg ccaaggcccg ttccctgaac gtcggccctt tgtccgacaa ccgttcccgc
     9301 ctgcccctga ttggcccgct gatcgaccgc tcccgcgtcc gttcgacggg cttcatggcg
     9361 cgcttcgaca cgacccgcga gcagatcgaa cacctggtca gcgaagtgca gaccacccag
     9421 caaggcatcg cgcagcgcaa tgcctcgctc gacgaaatgt tcgcagccgt gcgcgaggaa
     9481 caccgccttc ttggcgtcca catcgcggcc ggcaaggtcc gccttgccga gctgcgcgag
     9541 caggccgagg gtctgcgcgg caatgtcggg aacgaccctg gccgcgtgca ggagctggcc
     9601 gacctcgatg cgatggttgc caacctggac aagcgcatcg gcgacctgat cgccttgcaa
     9661 cattcggcca tgcagagcct gccgaccatc cgcatgatcc aggccaacaa ccagatgctg
     9721 gtcgataaat tccacaccat ccgcgaaatc accgtgccgg cgtggaagcg gcaattcatg
     9781 ctggccttga gcctcaacga gcagaagaac gccgtcgaac tggccacggc catcgacgac
     9841 accaccaacg acctgatgaa gcgcaatgcg gccctgctgc atcgcacgtc cgtcgaaacg
     9901 gcgaaggaga accaacgcct ggtgatcgac gtggacacgc tcaagcaggt tcagacgacg
     9961 ctcatcaaga ccgtcgagga cgttattcgc atccagcagg aaggcgtgca gaagcgcaag
    10021 gatgccgaga agcagatcgc cgcaatgcgt ggcgatcttc aagccaagct gacccgccag
    10081 cccgtgcgcg agctggccca acaggagtcc gtatgaatgc cacaaacacc gatgttttcg
    10141 cccaggtagg cggcctggag gcccgaggcg cgaagatgaa gaagcggggc acccgcttcc
    10201 tcatcgcggc gctggcagtc cttgccattg ccgggatcgg ggcagtaacg ggatgggcga
    10261 tcagcccgag cgcgacgccc ggaagcattg acgtgccgca ggtgctggca tcgacattca
    10321 gcgaccaggt gccgggcagt gagggcggcg gcctgggtgg cggcctgccc ttcacttcgg
    10381 ccgtcggggc attcacggac ttcatggcgg ggccggcaat ttttaccttg ggcattcttg
    10441 gcatagtggt cgcgggtgcc gtgctcgtgt tcgggggtga gttctgcggg ttcgtgcgat
    10501 ccgtctgcat gatggtgata gccgtcagca tgattttcgt gtcgtcgaac ttggtgaagg
    10561 gcattctcgg cggcgatcac gacgccggcc ctgcggagcc ttcgccgcgt gcgcgattca
    10621 tggcggccgt ggaggccaag gatttcgcgc gagtgcaaga gctgatcgag gcgcgtggag
    10681 ccaagtcggc ggctgattat gtccttgcgc agctcgccgt ggccgaaggt ctggaccgca
    10741 agcctggtgc gcgcgtcgtg gtcgggaaag cggcgggcag catggcaatg ccgcctgcgg
    10801 cgctgggttt tacgccaagg ggagaagcgg catacgccat cgagcggtca gcctatggtg
    10861 agccgaggtc cagcattgcg aagcagtacc agcaggaatg gaaccggaag gcggcgacct
    10921 ggtgggcgat ggccggtgtg gccggcatca tcggcgcgat cctggcggcg gcggcaaccg
    10981 gctttgttgg gctggcagtg tcgatccgca accgagtgaa gcgcgtgcgc gacctgttgg
    11041 tgatggagcc gggtgcagag ccataaggga tcgatccccc tcaagtcaaa agcctccggt
    11101 cggaggcttt tgactttctg ctcgaggagc taattccatt gagtaagttt ttaagcacat
    11161 cagcttcaaa agcgctctga agttcctata ctttctagag aataggaact tcggaatagg
    11221 tacttcaaga tccccaattc gagctcggta cccggggcgg ccgcttctag a
//
