LOCUS       rpo41                 8829 bp    DNA     circular SYN 15-JUN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             3117..7169
                     /note="pLannotate"
                     /label="RPO41"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(794..1222)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1630..2712
                     /note="pLannotate"
                     /label="lacI"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        7666..7757
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      7442..7528
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             7758..8618
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        1552..1629
                     /note="pLannotate"
                     /label="lacIq promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      join(8788..8829,1..546)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     terminator      7620..7647
                     /note="pLannotate"
                     /label="rrnB T2 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             3069..3089
                     /note="pLannotate"
                     /label="TEV site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    2981..2997
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     ncRNA           7323..7438
                     /note="pLannotate"
                     /label="5S rRNA"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="97.5"
                     /fragment="False"
                     /other="ncRNA"
     misc_feature    complement(1244..1295)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(732..783)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             3028..3047
                     /note="pLannotate"
                     /label="8xHis (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="83.3"
                     /fragment="True"
                     /other="CDS"
     promoter        2754..2771
                     /note="pLannotate"
                     /label="tet promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="62.1"
                     /fragment="True"
                     /other="promoter"
     misc_feature    complement(1328..1366)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="27.7"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(1468..1508)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="21.4"
                     /fragment="True"
                     /other="CDS"
     promoter        2943..2959
                     /note="pLannotate"
                     /label="EM7 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.4"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(2767..2868)
                     /note="pLannotate"
                     /label="yciV (fragment)"
                     /database="swissprot"
                     /identity="94.1"
                     /match_length="11.6"
                     /fragment="True"
                     /other="CDS"
     misc_feature    1296..1312
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(1227..1243)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="9.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccagggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      541 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      601 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      661 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      721 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
      781 ataattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
      841 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
      901 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
      961 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     1021 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     1081 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     1141 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     1201 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgctatggtg cactctcagt
     1261 acaatctgct ctgatgccgc atagttaagc cagtaccagt cacgtagcga tatcgctacg
     1321 tgactggtac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     1381 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     1441 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     1501 gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcatttacgt tgacaccatc
     1561 gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     1621 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     1681 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     1741 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
     1801 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
     1861 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
     1921 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
     1981 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
     2041 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
     2101 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
     2161 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
     2221 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
     2281 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
     2341 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
     2401 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
     2461 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
     2521 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     2581 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cacccaatac gcaaaccgcc
     2641 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     2701 agcgggcagt gagcgcaacg caattaatgt aagttagcgc gaattgatct ggtttgacag
     2761 cttatcatcg actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt
     2821 ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg
     2881 ttctggataa tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag
     2941 ctgttgacaa ttaatcatcc ggtccgtata atctgtggaa ttgtgagcgg ataacaattt
     3001 cacacaggaa acagaccatg tcgtactacc atcaccatca ccatcacgat tacgatatcc
     3061 caacgaccga aaacctgtat tttcagggcg ccatgggatc cggaattcaa aggcctatgc
     3121 tgagaccggc ctataaatcg ctcgtgaaaa catccctgtt acaaagacgg ttaatatctt
     3181 caaagggatc taagctgttt aaaccatctc cggactcaac aagtacaata ttaatatctg
     3241 aagaccccct tgtaacaggc tcgtccccaa cctcatctac aacatcaggt ataataagtt
     3301 caaatgattt ccctttgttc aacaaaaata ggaaagacgc caagagcagc atatcctacc
     3361 aatggaaaaa tccttcagaa ttagagtttg accctttcaa taaatcgcat gcctcagcag
     3421 taacctcgat gacaagaact agagacgtta tgcaattatg gtctctgtta gaagcatgct
     3481 tgcaatcgaa tctaatgaaa agagcgttct ctatattgga gtcactgtac ctggtacctg
     3541 aacataagca gcgatttatc gaggattata acatgtactt gaattcattt tccaaaaatg
     3601 accctaattt ccctatttta aaaatgaacg aaaaattaac aaatgatttg gagacaagtt
     3661 ttaaagacgt taattataat gacaaaactt tggcaataat gattcatcac gctttgaact
     3721 tccattcaac gacaagctcg atgcttttga agcctataat ctctgcctac ttgaaaatga
     3781 gcgtgaatgg tattagggaa attttctcct gcctagatat tttgaccatt tcagatctga
     3841 atattttaat gaatgactta aaggttataa caccatcgca actgccaaat tctgtaagac
     3901 caatattaga atcacttacg ctatctccta cacctgtaaa taatattgaa aatgaggagg
     3961 gtttgaacaa agtggaagct gagaacgatt caaaattgca taaagcatca aatgcaagct
     4021 ctgattctat aaaaaaacct tccttggatc ctcttcggga agtttcattc cacggttcaa
     4081 cagaagtctt gtcaaaagac gctgaaaagc ttatagctgt agacactatt ggaatgagag
     4141 taatccggca tactttgctg ggattatcat taacgccgga acagaaagag caaatttcta
     4201 aattcaaatt tgatgccaat gataatgttt tgaaaatgaa acctacgaaa aacgacgata
     4261 ataataattc tataaatttt tttgaaattt acaattcact gccaacatta gaggaaaaga
     4321 aggcattcga aagcgcatta aatatattta accaagatag acaaaaggta cttgaaaacc
     4381 gtgcaacgga agccgcaaga gagcgttgga agcacgattt tgaagaggca aaagctagag
     4441 gtgacatttc aattgaaaag aatttaaacg ttaagttgtg gaaatggtat aatgaaatgt
     4501 tacctctagt gaaggaagaa attaatcatt gtaggtcatt attgtcagaa aagctatcag
     4561 ataagaaagg cttgaataaa gttgacacga accgtcttgg atatggacca tacttaacct
     4621 tgattgaccc tggtaaaatg tgtgtcatca ccattttgga actattaaag ttaaattcta
     4681 cgggtggtgt tattgaaggt atgaggaccg caagagcggt tatatcagtg ggtaaagcta
     4741 ttgaaatgga atttagatcc gagcaggttt tgaaaagcga atctcaagcg ttcagagatg
     4801 ttaacaagaa atcaccagaa ttcaaaaaac tggtacaaaa tgctaaatct gtattcagat
     4861 catctcaaat tgagcagtcg aaaattttgt ggcctcagag tattagggcc agaatcggtt
     4921 cggtattaat ctcaatgttg attcaagtcg ctaaggtatc tgtgcaaggt gttgaccctg
     4981 tgacaaaagc taaggttcac ggtgaagctc cagcttttgc gcatggttat cagtaccaca
     5041 atggttccaa attgggtgtc ttgaagattc acaagacttt gattcgtcaa ttgaacggtg
     5101 aaagattgat tgcctctgtc caaccacaat tattaccaat gttggtggaa cccaaaccct
     5161 gggttaattg gagatcaggt ggatatcatt atacacaatc taccctttta agaacaaaag
     5221 actcacccga acaagtagca tatttgaaag ctgcttccga taatggtgat atagaccgcg
     5281 tttatgatgg tttgaatgtt ctgggtagaa caccgtggac tgtcaacaga aaagtttttg
     5341 atgtggtttc acaagtttgg aataaaggtg aaggtttcct agatattcca ggtgcacaag
     5401 acgaaatggt tttaccacca gctcctccga agaactccga cccttccatt ttgagagcct
     5461 ggaaacttca agtcaaaaca attgctaata aattctcttc agatagatca aatagatgtg
     5521 atacgaacta caaattggaa attgcaagag catttttggg tgaaaagctg tactttccac
     5581 ataatttgga ctttagggga cgtgcttatc ctttgtctcc acatttcaat catcttggta
     5641 atgatatgag tcgtggcctt ttgatttttt ggcacggtaa aaaattagga ccttctggtc
     5701 tcaagtggtt aaagattcat ttgtcaaatc tgttcggatt tgataagtta cccctaaagg
     5761 atcgtgttgc atttactgag tctcatttgc aggacatcaa ggattctgcg gaaaatccct
     5821 taacgggaga tcgttggtgg actactgctg ataagccatg gcaagcattg gccacatgct
     5881 ttgaattgaa tgaagttatg aaaatggata atccagagga gtttatctct catcaaccgg
     5941 ttcaccaaga cggtacttgc aatggtttgc aacattatgc tgccttgggt ggtgacgttg
     6001 aaggggctac tcaagtgaat ctggttccaa gcgataaacc acaagatgtt tatgctcatg
     6061 ttgcaaggtt ggtgcaaaaa agattagaaa ttgcagcaga aaaaggagac gaaaacgcca
     6121 aaattttgaa ggataagatt accagaaagg ttgtcaagca aaccgtcatg acaaacgttt
     6181 atggtgttac ctacgtcggg gctacttttc aaattgctaa acaattaagc ccaatatttg
     6241 atgatcgtaa agaaagctta gacttttcta aatacctaac caaacatgtt tttagtgcta
     6301 tccgcgagtt atttcatagt gcgcatttaa ttcaggattg gttaggtgaa agtgcgaaaa
     6361 gaatttccaa atctatcagg ttggatgttg atgaaaaatc gttcaagaac ggtaataaac
     6421 ccgactttat gtcatccgtc atatggacga caccactagg actaccaatt gttcagccat
     6481 atcgtgaaga aagcaaaaag caagttgaga ccaatttgca aactgtcttt attagcgatc
     6541 ctttcgctgt caaccctgtt aatgcaagaa ggcaaaaggc aggtcttcca ccgaacttca
     6601 tccattcgct ggatgcttca catatgttgc taagtgcagc agaatgtgga aagcaaggat
     6661 tagatttcgc atcagttcat gattcttact ggacgcatgc atctgatata gacactatga
     6721 atgtggtgtt aagggaacag tttatcaaac tccatgaggt agatctggtt ttaagattga
     6781 aggaggagtt tgaccaaagg tataagaatt acgtcaagat tggaaagttg aagagatcta
     6841 cggatttggc tcaaaagatt attagaatcc gtaaagattt atccagaaaa ttaggaagaa
     6901 gtactacatt agcagatgaa atttattttg agaaaaaaag gcaagaatta ttaaacagcc
     6961 ctctaataga agatagaaat gttggtgaaa agatggttac aactgtatca ttatttgaag
     7021 acatcactga cttggacgca cttgaactag aaaacggtgg agatgaaaat agcggtatga
     7081 gtgttctttt accgttgaga ttacctgaaa tcccacctaa aggtgatttt gatgttacag
     7141 tattgagaaa cagtcaatat tttttctcgt gagagctcac tagtcgcggc cgctttcgaa
     7201 tctagagcct gcagtctcga ggcatgcggt accaagcttg gctgttttgg cggatgagag
     7261 aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat
     7321 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa
     7381 cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca
     7441 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     7501 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca
     7561 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca
     7621 gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct
     7681 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7741 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7801 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7861 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7921 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7981 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     8041 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8101 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8161 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8221 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8281 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8341 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8401 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8461 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8521 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8581 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8641 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8701 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8761 accccgtaga aaagatcaaa ggatctcttg agatcctttt tttctgcgcg taatctgctg
     8821 cttgcaaac
//
