LOCUS       t7rnap_C_L_V174C                 5657 bp    DNA     circular SYN 15-JUN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             4586..5446
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(732..872)
                     /note="pLannotate"
                     /label="bom"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             1326..3974
                     /note="pLannotate"
                     /label="RPOL_BPT7"
                     /database="swissprot"
                     /identity="99.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        4481..4585
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      join(5616..5657,1..546)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        1101..1131
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(4340..4368)
                     /note="pLannotate"
                     /label="tet promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     RBS             1228..1250
                     /note="pLannotate"
                     /label="RBS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     protein_bind    1065..1086
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             1296..1313
                     /note="pLannotate"
                     /label="thrombin site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    1139..1155
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(3998..4292)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="24.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(1192..1221)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="52.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(974..1016)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="22.4"
                     /fragment="True"
                     /other="CDS"
     CDS             1269..1285
                     /note="pLannotate"
                     /label="capTEV™ (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.0"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg ggggttcgtg cacacagccc
      301 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      361 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      421 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      541 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      601 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      661 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      721 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
      781 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
      841 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
      901 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
      961 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctggcac
     1021 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     1081 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     1141 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
     1201 tgcctgcagg tcgactctag aaataatttt gtttaacttt aagaaggaga tataccatgg
     1261 gcagcagcca tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgc
     1321 tcgagatgaa cacgattaac atcgctaaga acgacttctc tgacatcgaa ctggctgcta
     1381 tcccgttcaa cactctggct gaccattacg gtgagcgttt agctcgcgaa cagttggccc
     1441 ttgagcatga gtcttacgag atgggtgaag cacgcttccg caagatgttt gagcgtcaac
     1501 ttaaagctgg tgaggttgcg gataacgctg ccgccaagcc tctcatcact accctactcc
     1561 ctaagatgat tgcacgcatc aacgactggt ttgaggaagt gaaagctaag cgcggcaagc
     1621 gcccgacagc cttccagttc ctgcaagaaa tcaagccgga agccgtagcg tacatcacca
     1681 ttaagaccac tctggctagc ctaaccagtg ctgacaatac aaccgttcag gctgtagcaa
     1741 gcgcaatcgg tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt gaccttgaag
     1801 ctaagcactt caagaaaaac gttgaggaac aactcaacaa gcgctgcggg cacgtctaca
     1861 agaaagcatt tatgcaagtt gtcgaggctg acatgctctc taagggtcta ctcggtggcg
     1921 aggcgtggtc ttcgtggcat aaggaagact ctattcatgt aggagtacgc tgcatcgaga
     1981 tgctcattga gtcaaccgga atggttagct tacaccgcca aaatgctggc gtagtaggtc
     2041 aagactctga gactatcgaa ctcgcacctg aatacgctga ggctatcgca acccgtgcag
     2101 gtgcgctggc tggcatctct ccgatgttcc aaccttgcgt agttcctcct aagccgtgga
     2161 ctggcattac tggtggtggc tattgggcta acggtcgtcg tcctctggcg ctggtgcgta
     2221 ctcacagtaa gaaagcactg atgcgctacg aagacgttta catgcctgag gtgtacaaag
     2281 cgattaacat tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta gcggtcgcca
     2341 acgtaatcac caagtggaag catagcccgg tcgaggacat ccctgcgatt gagcgtgaag
     2401 aactcccgat gaaaccggaa gacatcgaca tgaatcctga ggctctcacc gcgtggaaac
     2461 gtgctgccgc tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt atcagccttg
     2521 agttcatgct tgagcaagcc aataagtttg ctaaccataa ggccatctgg ttcccttaca
     2581 acatggactg gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa ggtaacgata
     2641 tgaccaaagg actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa ggttactact
     2701 ggctgaaaat ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc cctgagcgca
     2761 tcaagttcat tgaggaaaac cacgagaaca tcatggcttg cgctaagtct ccactggaga
     2821 acacttggtg ggctgagcaa gattctccgt tcagcttcct tgcgttcagc tttgagtacg
     2881 ctggggtaca gcaccacggc ctgagctata acagctccct tccgctggcg tttgacgggt
     2941 cttgctctgg catccagcac ttctccgcga tgctccgaga tgaggtaggt ggtcgcgcgg
     3001 ttaacttgct tcctagtgaa accgttcagg acatctacgg gattgttgct aagaaagtca
     3061 acgagattct acaagcagac gcaatcaatg ggaccgataa cgaagtagtt accgtgaccg
     3121 atgagaacac tggtgaaatc tctgagaaag tcaagctggg cactaaggca ctggctggtc
     3181 aatggctggc ttacggtgtt actcgcagtg tgactaagcg ttcagtcatg acgctggctt
     3241 acgggtccaa agagttcggc ttccgtcaac aagtgctgga agataccatt cagccagcta
     3301 ttgattccgg caagggtctg atgttcactc agccgaatca ggctgctgga tacatggcta
     3361 agctgatttg ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca atgaactggc
     3421 ttaagtctgc tgctaagctg ctggctgctg aggtcaaaga taagaagact ggagagattc
     3481 ttcgcaagcg tagcgctgtg cattgggtaa ctcctgatgg tttccctgtg tggcaggaat
     3541 acaagaagcc tattcagacg cgcttgaacc tgatgttcct cggtcagttc cgcttacagc
     3601 ctaccattaa caccaacaaa gatagcgaga ttgatgcaca caaacaggag tctggtatcg
     3661 ctcctaactt tgtacacagc caagacggta gccaccttcg taagactgta gtgtgggcac
     3721 acgagaagta cggaatcgaa tcttttgcac tgattcacga ctccttcggt accattccgg
     3781 ctgacgctgc gaacctgttc aaagcagtgc gcgaaactat ggttgacaca tatgagtcta
     3841 gcgatgtact ggctgatttc tacgaccagt tcgctgacca gttgcacgag tctcaattgg
     3901 acaaaatgcc agcacttccg gctaaaggta acttgaacct ccgtgacatc ttagagtcgg
     3961 acttcgcgtt cgcgtaacgc caaatcaata cgactccgga tccacaggac gggtgtggtc
     4021 gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg
     4081 ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat
     4141 agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag
     4201 aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg
     4261 aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt
     4321 aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc
     4381 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4441 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4501 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4561 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4621 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4681 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4741 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4801 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     4861 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4921 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4981 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5041 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5101 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     5161 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5221 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5281 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5341 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5401 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5461 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5521 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5581 agcgtcagac cccgtagaaa agatcaaagg atctcttgag atcctttttt tctgcgcgta
     5641 atctgctgct tgcaaac
//
