LOCUS       Exported                2313 bp ds-DNA     linear   UNA 11-MAY-2023
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Jun 30, 2023 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     32..48
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        62..390
                     /label=PvirB
     CDS             413..1435
                     /codon_start=1
                     /label=AtVIP1
                     /translation="MEGGGRGPNQTILSEIEHMPEAPRQRISHHRRARSETFFSGESID
                     DLLLFDPSDIDFSSLDFLNAPPPPQQSQQQPQASPMSVDSEETSSNGVVPPNSLPPKPE
                     ARFGRHVRSFSVDSDFFDDLGVTEEKFIATSSGEKKKGNHHHSRSNSMDGEMSSASFNI
                     ESILASVSGKDSGKKNMGMGGDRLAELALLDPKRAKRILANRQSAARSKERKIRYTGEL
                     ERKVQTLQNEATTLSAQVTMLQRGTSELNTENKHLKMRLQALEQQAELRDALNEALRDE
                     LNRLKVVAGEIPQGNGNSYNRAQFSSQQSAMNQFGNKTNQQMSTNGQPSLPSYMDFTKR
                     G"
     CDS             1448..1924
                     /codon_start=1
                     /label=VirF-mutN42
                     /translation="ESAENIANFSKSHRFTRDAVRTEPLEKFSSRLKILSRNAKLLSHA
                     VRHAATLPDGEQLSEAQLSQMRSEVATRPVLGVAYTHQDGQPEERLSGNHLDHKINNIP
                     NLVFNVAEPIMFNEISALEVMAEVRPIARSIKTAHDDARAELMSADRPRSTRGL"
     promoter        complement(1963..1981)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2002..2018)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2026..2042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2050..2080)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2095..2116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgaattggag ctccaccgcg gtggcggccg ctctagaact agtggatccc ccgctgggac
       61 ataggatagt cggcataggc ggataccctc gccacgcaaa tggcgcgatc gacaccctga
      121 tcggagcctg cgaagtgcca cagtttattg gaaccatccc ccgttcattc cgccctggaa
      181 tttcacttcg acggcattct agatcctcgc cgttgtctta gagagccatc tttcgtcggc
      241 gcacccttaa gtgcatggaa agccgttttc gcttcaaatg aaatcgaaaa gaagaaaaca
      301 aaaatcctag agtaaccgac cctcccgata atcgtgaaca tcagatgaca gcatttcttc
      361 cgaccgaagt ggctgtgttg gttatgagct tggggagatt gcatatggtc gaatggaagg
      421 aggaggaaga ggaccaaatc aaacgattct cagtgaaata gaacatatgc ctgaagctcc
      481 acgtcaacgt atctctcatc accgtcgagc tcgctctgaa accttcttct ccggcgaatc
      541 aatcgacgat ctcctcttat tcgatccttc cgatatcgat ttctcttctc tagacttcct
      601 caacgctcca ccaccaccac aacaatcaca acaacaaccg caagcttctc ccatgtccgt
      661 tgattcggaa gaaacctcat cgaacggtgt tgttcctcct aattctcttc ctccaaaacc
      721 cgaagctaga ttcggtcgcc atgttcgtag cttctcggtt gattccgatt tcttcgatga
      781 tttgggtgtt actgaggaga agtttatagc tacaagttca ggagagaaga agaaagggaa
      841 tcatcatcat agcaggagta attctatgga tggagagatg agttcggcgt cgtttaatat
      901 cgaatcgatt ttagcttctg tgagtggtaa agatagtggg aagaagaata tgggtatggg
      961 tggtgataga cttgctgagc ttgctttgct tgatcctaaa agagctaaaa ggattttagc
     1021 gaatagacaa tctgcggcga ggtcgaaaga gaggaagatt aggtatactg gtgagttaga
     1081 gaggaaggtt cagacacttc agaatgaagc tactacattg tctgctcaag tcactatgtt
     1141 acagagagga acatcagagc tgaacactga aaataaacac ctcaaaatgc ggcttcaagc
     1201 tttagagcaa caagctgaac ttagggatgc tttgaatgaa gcgctgcggg atgaactgaa
     1261 ccgacttaag gtggtagctg gagaaattcc tcaggggaat ggaaattctt acaaccgtgc
     1321 tcaattctca tctcagcaat cggcaatgaa tcagtttggg aacaaaacga accaacagat
     1381 gagtacaaac gggcagccat cgctcccaag ctacatggat ttcaccaaga gaggcggtac
     1441 ccctgtcgag tcggctgaga atatagctaa ttttagcaaa tcccatcgtt tcacaaggga
     1501 cgcggttcga acagaacctt tagaaaaatt ctcttcacgc ctcaaaatcc tctcgcgaaa
     1561 cgcgaaattg ttgtctcatg ccgtgcggca cgcagcaaca cttccagatg gagaacaatt
     1621 aagtgaagcg caactgtcgc aaatgcgaag tgaagtcgct actcgcccag tcctgggcgt
     1681 agcgtatacc catcaagacg gtcaaccaga agagaggttg tcagggaacc acttggacca
     1741 taaaatcaac aatatcccta acttggtctt caacgttgcg gaaccgatta tgttcaatga
     1801 aatctccgct ctcgaggtta tggcagaagt tcggcccata gcccgatcca ttaaaacggc
     1861 tcacgacgat gcgcgagcgg aattaatgtc ggcggacaga cctcgatcaa cgcgcggtct
     1921 atgatcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt gagggttaat
     1981 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2041 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2101 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2161 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2221 catgcataaa aactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     2281 ggcatgatga acctgaatcg ccagcggcat cag
//
