LOCUS       pAAV-hSyn-LMO7        6806 bp DNA     circular SYN 30-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Hochgeschwender
  TITLE     Direct Submission
  JOURNAL   Exported Jun 30, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6806
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     repeat_region   5..145
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        190..637
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     419..441
                     /label=D134
     CDS             651..701
                     /codon_start=1
                     /label=Gluc Signal Sequence
                     /translation="MGVKVLFALICIAVAEA"
     CDS             702..1379
                     /codon_start=1
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTG"
     primer_bind     complement(767..787)
                     /label=C404
     primer_bind     complement(822..842)
                     /label=C405
     regulatory      954..963
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1380..1880
                     /codon_start=1
                     /label=eKL9h
                     /translation="FEDFVGDWEQTAAYNLDQVLEQGGVSSVLQTLAVSVTPIQRIVRS
                     GENGLKIDIHVIIPYEGLSADQMAHIEEVFKVVYPVDDHHFKVIMEYGTLVIDGVTPNM
                     LNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLFRVTINGVSGWRLFE
                     KISN"
     primer_bind     1761..1781
                     /label=C402
     CDS             1881..1925
                     /codon_start=1
                     /label=15 aa Linker
                     /translation="GDPLVQCGGIAGSAT"
     CDS             1926..2825
                     /codon_start=1
                     /label=VChR1
                     /translation="MDYPVARSLIVRYPTDLGNGTVCMPRGQCYCEGWLRSRGTSIEKT
                     IAITLQWVVFALSVACLGWYAYQAWRATCGWEEVYVALIEMMKSIIEAFHEFDSPATLW
                     LSSGNGVVWMRYGEWLLTCPVLLIHLSNLTGLKDDYSKRTMGLLVSDVGCIVWGATSAM
                     CTGWTKILFFLISLSYGMYTYFHAAKVYIEAFHTVPKGICRELVRVMAWTFFVAWGMFP
                     VLFLLGTEGFGHISPYGSAIGHSILDLIAKNMWGVLGNYLRVKIHEHILLYGDIRKKQK
                     ITIAGQEMEVETLVAEEED"
     primer_bind     complement(2011..2034)
                     /label=C71/D178
     CDS             2889..2906
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    2937..3525
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2990..3010)
                     /label=C65
     CDS             complement(3408..3419)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3557..4033
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4073..4213
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4288..4743
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5025..5129
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5130..5990
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6161..6749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg
       61 cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg
      121 gccaactcca tcactagggg ttcctgcggc cgcacgcgtg tgtctagact gcagagggcc
      181 ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct
      241 gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc
      301 cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc
      361 agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac
      421 tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg
      481 tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg
      541 gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt
      601 gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcg agaaggtacc atgggagtca
      661 aagttctgtt tgccctgatc tgcatcgctg tggccgaggc cgtgtctaaa ggagaggagg
      721 ataatatggc tagtctgccc gctactcacg agctgcacat ttttgggagc atcaacgggg
      781 tggattttga catggtgggc cagggcacag gcaaccctaa tgatggctac gaggagctga
      841 atctgaagtc caccaagggc gacctgcagt tttctccttg gatcctggtg ccacacatcg
      901 gctatggctt ccaccagtat ctgccctacc ctgacggcat gtctccattt caggccgcca
      961 tggtggatgg cagcggctac caggtgcacc ggaccatgca gttcgaggac ggcgccagcc
     1021 tgacagtgaa ctacagatat acctacgagg gctcccacat caagggcgag gcccaggtga
     1081 agggcacagg ctttccagcc gatggccccg tgatgacaaa cagcctgacc gccgccgact
     1141 ggtgcagatc caagaagacc taccccaatg ataagacaat catctctacc ttcaagtgga
     1201 gctataccac aggcaacggc aagcggtaca gatctacagc caggaccaca tatacctttg
     1261 ccaagccaat ggccgccaac tatctgaaga atcagcccat gtacgtgttc cgcaagacag
     1321 agctgaagca cagcaagacc gagctgaact tcaaggagtg gcagaaggcc ttcacaggct
     1381 ttgaggactt cgtgggcgat tgggagcaga ccgccgccta taacctggat caggtgctgg
     1441 agcagggagg cgtgagctcc gtgctgcaga cactggccgt gtccgtgacc cctatccaga
     1501 ggatcgtgcg ctctggcgag aatggcctga agatcgacat ccacgtgatc atcccatacg
     1561 agggcctgtc cgccgatcag atggcccaca tcgaggaggt gtttaaggtg gtgtaccccg
     1621 tggacgatca ccacttcaaa gtgatcatgg agtatggcac cctggtcatc gacggagtga
     1681 ccccaaacat gctgaattat tttggccggc cttacgaggg catcgccgtg ttcgatggca
     1741 agaagatcac cgtgacaggc accctgtgga acggcaataa gatcatcgac gagcggctga
     1801 tcacccctga tggctctatg ctgttcagag tgaccatcaa cggcgtgagc ggctggcggc
     1861 tgttcgagaa gatttcaaac ggcgacccac tagtccagtg tggtggaatt gccggatccg
     1921 ccaccatgga ttaccctgtt gctagaagcc tcatagttcg ctacccaacc gacctcggaa
     1981 acggcaccgt ctgcatgcca agaggacagt gttactgtga aggttggctt cggagtcgcg
     2041 gcacttccat tgaaaagaca atagcaatta ctcttcagtg ggtagtcttt gctttgtcag
     2101 tggcttgcct ggggtggtat gcgtatcaag cgtggcgagc tacctgcgga tgggaggagg
     2161 tttacgtagc cttgatagaa atgatgaaaa gcatcatcga ggccttccac gagttcgaca
     2221 gccctgcaac actgtggctg tcttcaggga acggcgtagt ttggatgcgg tatggcgaat
     2281 ggctcctcac ctgcccggtc cttctgatcc atctgagcaa cctcacaggc ctgaaggacg
     2341 attatagcaa aaggactatg ggcctgttgg tttctgatgt gggatgcatc gtgtggggcg
     2401 caaccagcgc catgtgtacg gggtggacga agatcctgtt cttcctcatc tcattgagct
     2461 atggtatgta tacctatttt catgctgcta aagtttatat cgaagcattc cacacagttc
     2521 caaaagggat ttgtcgagaa ctggtccgag tgatggcctg gacattcttt gtggcttggg
     2581 gaatgtttcc agtcctgttt ctgctgggca cggaaggatt cggtcatatc agcccttatg
     2641 gatctgccat tgggcactcc atcctcgacc tgattgcaaa gaacatgtgg ggtgtgctgg
     2701 ggaattacct gcgcgtcaaa atccacgagc acatcctgtt gtatggcgac atcagaaaga
     2761 agcagaaaat tacgatcgcc ggccaagaga tggaggttga gacactggtg gctgaagagg
     2821 aggacgcggc cgcctaacat atggaattca ccggtggcgc gccaatatta tgctagccaa
     2881 ttgccggtca tcatcaccat caccattgag tttaattcga tatcaagctt atcgataatc
     2941 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3001 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3061 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     3121 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     3181 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3241 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3301 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     3361 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     3421 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3481 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc
     3541 tgctcgagag atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct
     3601 ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt
     3661 gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg
     3721 gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc
     3781 agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc
     3841 tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt
     3901 tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca
     3961 ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc
     4021 cttccctgtc cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc
     4081 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4141 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4201 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4261 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     4321 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4381 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4441 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4501 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4561 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4621 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4681 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     4741 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     4801 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4861 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4921 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4981 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5041 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5101 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5161 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5221 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5281 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5341 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5401 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5461 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5521 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5581 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5641 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5701 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5761 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5821 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5881 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5941 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6001 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6061 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6121 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6181 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6241 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6301 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6361 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6421 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6481 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6541 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6601 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6661 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6721 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6781 tggccttttg ctggcctttt gctcac
//
