LOCUS       Exported                3363 bp DNA     circular SYN 23-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..8
                     /note="/dnas_title=pYPQ141B"
     source          9..3363
                     /note="/dnas_title=pYPQ141B"
     source          355..499
                     /note="/dnas_title=pYPQ141D2.0"
     source          683..717
                     /note="/dnas_title=pYPQ141B"
     promoter        29..354
                     /label=AtU3 promoter
                     /note="AtU3 promoter"
     misc_feature    290..299
                     /label=USE
                     /note="USE"
     TATA_signal     324..331
                     /note="TATA box"
     misc_feature    355..454
                     /label=ge4.1_5'
     misc_structure  455..487
                     /label=guide RNA cloning site
                     /note="guide RNA cloning site"
     misc_feature    488..499
                     /label=ge4.1_3'
     terminator      500..514
                     /label=extra terminator
                     /note="AtU3 terminator"
     misc_feature    535..634
                     /label=attL2
                     /note="/ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff0080
                     /dnas_title=attL2
                     /vntifkey=21"
     primer_bind     complement(651..672)
                     /label=T7
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green
                     /dnas_title=T7
                     /vntifkey=28"
     primer_bind     complement(683..717)
                     /label=M13-rev
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green
                     /dnas_title=M13-rev
                     /vntifkey=28"
     misc_feature    804..937
                     /label=Spn promoter
                     /note="/ApEinfo_fwdcolor=#2fec13
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#2fec13
                     /dnas_title=Spn promoter
                     /vntifkey=21"
     misc_feature    938..1948
                     /label=SpnR
                     /note="/ApEinfo_fwdcolor=#f70938
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#f70938
                     /dnas_title=SpnR
                     /vntifkey=21"
     misc_feature    2015..2688
                     /label=pUC origin
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green
                     /dnas_title=pUC origin
                     /vntifkey=21"
     misc_feature    3211..3234
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="M13-F1"
     primer_bind     3227..3244
                     /label=M13-fwd
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green
                     /dnas_title=M13-fwd
                     /vntifkey=28"
     misc_feature    3246..3358
                     /label=attL5
                     /note="/ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#c0c0c0
                     /dnas_title=attL5
                     /vntifkey=21"
ORIGIN
        1 cgttccctct agataacgca ggatcctatt tactttaaat ttttcttatg gctcagcctg
       61 tgatggataa ctgaatcaaa caaatggcgt ctgggtttaa gaacatctgt tttggctatg
      121 ttggacgaaa caagtgaact tttaggatca acttccgttt atatacggag cttatatcga
      181 gcaataagat aagtgggctt tttatgtaat ttaatgggct atcgtccata tattcactaa
      241 tacccatgcc cagtacccat gtatgcgttt catataagct cctaatttct cccacatcgc
      301 tcaaatctaa acaaatcttg ttgtatatat aacactgagg gagcaccatt ggtcaccgct
      361 tcacttagag tgaaggtggg ctgcttgcat cagcctaatg tcgagaagtg ctttcttcgg
      421 aaagtaaccc tcgaaacaaa gaaaggaatg caacggagac gagatctagt ctgagtcgac
      481 cgtctctttt tatttttttt ccctttcctt ttttaactag taagggcgaa ttcgacccag
      541 ctttcttgta caaagttggc attataaaaa ataattgctc atcaatttgt tgcaacgaac
      601 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
      661 agtcgtatta catggtcata gcatccccta tagtgagtcg tattacatgg tcatagctgt
      721 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
      781 tatatcatca tgcctcctct ggaccagcca ggacagaaat gcctcgactt cgctgctgcc
      841 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
      901 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
      961 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     1021 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     1081 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     1141 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     1201 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     1261 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     1321 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     1381 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     1441 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     1501 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     1561 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     1621 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     1681 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     1741 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     1801 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     1861 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     1921 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     1981 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2041 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2101 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2161 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     2221 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2281 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2341 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2401 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
     2461 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2521 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2581 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2641 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2701 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     2761 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     2821 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     2881 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctcgccagga
     2941 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
     3001 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     3061 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     3121 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     3181 tactctcgcg ttaacgcttg catggatgtt ttcccagtca cgacgttgta aaacgacggc
     3241 cagtcttaag ctcgggccca aataatgatt ttattttgac tgatagtgac ctgttcgttg
     3301 caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa agttgcccca
     3361 tgg
//
