LOCUS       pcDNA-puro-tagBF        8742 bp DNA     circular SYN 03-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA-puro GFP-24xMS2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Jieli Wei
  TITLE     Direct Submission
  JOURNAL   Exported Jul 3, 2023 from SnapGene 6.1.1
            https://www.snapgene.com
COMMENT     http://www.informaxinc.com/
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..8742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1625..3122
                     /chromosome="7"
                     /map="7p22.1"
                     /mol_type="mRNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:9606"
                     /organism="Homo sapiens"
     misc_feature    905..1612
                     /label=TagBFP
                     /note="/vntifkey=21"
                     /note="color: #0000ff; direction: RIGHT"
     CDS             1625..2749
                     /codon_start=1
                     /gene="ACTB"
                     /gene_synonym="BRWS1; PS1TP5BP1"
                     /product="actin, cytoplasmic 1"
                     /label=ACTB
                     /note="beta cytoskeletal actin; PS1TP5-binding protein 1; 
                     I(2)-actin"
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS5341.1"
                     /db_xref="GeneID:60"
                     /db_xref="HGNC:HGNC:132"
                     /db_xref="MIM:102630"
                     /protein_id="NP_001092.1"
                     /translation="DDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG
                     MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL
                     TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVP
                     IYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDF
                     EQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSI
                     MKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWI
                     GGSILASLSTFQQMWISKQEYDESGPSIVHRKCF"
     misc_feature    2750..3122
                     /label=3'UTR
                     /note="color: #a6acb3"
     misc_feature    3146..4553
                     /label=24xMS2
                     /note="/vntifkey=21"
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    3194..3212
                     /label=MS2 site
                     /label=MS2\site
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aaccatgagc gagctgatta aggagaacat gcacatgaag ctctacatgg agggcaccgt
      961 ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     1021 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     1081 tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt
     1141 cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
     1201 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     1261 caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg
     1321 ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga
     1381 catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga ccacatatag
     1441 atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact
     1501 ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc
     1561 cagatactgc gacctcccta gcaaactggg gcacaagctg aatctgtaca agaagcttgg
     1621 taccgatgat gatatcgccg cgctcgtcgt cgacaacggc tccggcatgt gcaaggccgg
     1681 cttcgcgggc gacgatgccc cccgggccgt cttcccctcc atcgtggggc gccccaggca
     1741 ccagggcgtg atggtgggca tgggtcagaa ggattcctat gtgggcgacg aggcccagag
     1801 caagagaggc atcctcaccc tgaagtaccc catcgagcac ggcatcgtca ccaactggga
     1861 cgacatggag aaaatctggc accacacctt ctacaatgag ctgcgtgtgg ctcccgagga
     1921 gcaccccgtg ctgctgaccg aggcccccct gaaccccaag gccaaccgcg agaagatgac
     1981 ccagatcatg tttgagacct tcaacacccc agccatgtac gttgctatcc aggctgtgct
     2041 atccctgtac gcctctggcc gtaccactgg catcgtgatg gactccggtg acggggtcac
     2101 ccacactgtg cccatctacg aggggtatgc cctcccccat gccatcctgc gtctggacct
     2161 ggctggccgg gacctgactg actacctcat gaagatcctc accgagcgcg gctacagctt
     2221 caccaccacg gccgagcggg aaatcgtgcg tgacattaag gagaagctgt gctacgtcgc
     2281 cctggacttc gagcaagaga tggccacggc tgcttccagc tcctccctgg agaagagcta
     2341 cgagctgcct gacggccagg tcatcaccat tggcaatgag cggttccgct gccctgaggc
     2401 actcttccag ccttccttcc tgggcatgga gtcctgtggc atccacgaaa ctaccttcaa
     2461 ctccatcatg aagtgtgacg tggacatccg caaagacctg tacgccaaca cagtgctgtc
     2521 tggcggcacc accatgtacc ctggcattgc cgacaggatg cagaaggaga tcactgccct
     2581 ggcacccagc acaatgaaga tcaagatcat tgctcctcct gagcgcaagt actccgtgtg
     2641 gatcggcggc tccatcctgg cctcgctgtc caccttccag cagatgtgga tcagcaagca
     2701 ggagtatgac gagtccggcc cctccatcgt ccaccgcaaa tgcttctagg cggactatga
     2761 cttagttgcg ttacaccctt tcttgacaaa acctaacttg cgcagaaaac aagatgagat
     2821 tggcatggct ttatttgttt tttttgtttt gttttggttt tttttttttt tttggcttga
     2881 ctcaggattt aaaaactgga acggtgaagg tgacagcagt cggttggagc gagcatcccc
     2941 caaagttcac aatgtggccg aggactttga ttgcacattg ttgttttttt aatagtcatt
     3001 ccaaatatga gatgcgttgt tacaggaagt cccttgccat cctaaaagcc accccacttc
     3061 tctctaagga gaatggccca gtcctctccc aagtccacac aggggaggtg atagcattgc
     3121 ttggatccac tagtccagtg tggtggaatt cgggccctat atatcgatcc taaggtacct
     3181 aattgcctag aaaacatgag gatcacccat gtctgcaggt cgactccaga aaacatgagg
     3241 atcacccatg tctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga
     3301 aaacatgagg atcacccatg tctgcaggtc gactctagaa aacatgagga tcacccatgt
     3361 ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa aacatgagga
     3421 tcacccatgt ctgcaggtcg actccagaaa acatgaggat cacccatgtc tgcagtattc
     3481 ccgggttcat tagatcctaa ggtacctaat tgcctagaaa acatgaggat cacccatgtc
     3541 tgcaggtcga ctctagaaaa catgaggatc acccatgtct gcagtattcc cgggttcatt
     3601 agatcctaag gtacctaatt gcctagaaaa catgaggatc acccatgtct gcaggtcgac
     3661 tccagaaaac atgaggatca cccatgtctg cagtattccc gggttcatta gatcctaagg
     3721 tacctaattg cctagaaaac atgaggatca cccatgtctg caggtcgact ctagaaaaca
     3781 tgaggatcac ccatgtctgc agtattcccg ggttcattag atcctgcgcg cgatcggatc
     3841 agggccctat atatcgatct aaggtaccga attgcctaga aaacatgagg atcacccatg
     3901 tctgcaggtc gactccagaa aacatgagga tcacccatgt ctgcagtatt cccgggttca
     3961 ttagatccta aggtacctaa ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg
     4021 actctagaaa acatgaggat cacccatgtc tgcagtattc ccgggttcat tagatcctaa
     4081 ggtacctaat tgcctagaaa acatgaggat cacccatgtc tgcaggtcga ctccagaaaa
     4141 catgaggatc acccatgtct gcagtattcc cgggttcatt agatcctaag gtacctaatt
     4201 gcctagaaaa catgaggatc acccatgtct gcaggtcgac tctagaaaac atgaggatca
     4261 cccatgtctg cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaaac
     4321 atgaggatca cccatgtctg caggtcgact ctagaaaaca tgaggatcac ccatgtctgc
     4381 agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaaaca tgaggatcac
     4441 ccatgtctgc aggtcgactc cagaaaacat gaggatcacc catgtctgca gtattcccgg
     4501 gttcattaga tctgcgcgcg atcggatccg agctcggtac caagcttctc gagtctagag
     4561 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     4621 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     4681 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     4741 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     4801 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     4861 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4921 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4981 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5041 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5101 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5161 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5221 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5281 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5341 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5401 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5461 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5521 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     5581 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     5641 aaccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     5701 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     5761 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     5821 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     5881 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     5941 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     6001 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     6061 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     6121 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     6181 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     6241 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     6301 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     6361 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     6421 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6481 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6541 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6601 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6661 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6721 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6781 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6841 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6901 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7501 ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga
     7561 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7621 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7681 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7741 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7801 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7861 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7921 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7981 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8041 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8101 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8161 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8221 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8281 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8341 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8401 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8461 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8521 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8581 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8641 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8701 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
