LOCUS       pcDNA-tdMCP-msfG        7043 bp DNA     circular SYN 03-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3.1-MS2-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   mskcc
  TITLE     Direct Submission
  JOURNAL   Exported Jul 3, 2023 from SnapGene 6.1.1
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..7043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          924..1642
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          1643..1660
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    938..1642
                     /label=tdMCP
                     /note="color: #ffff99"
     CDS             1715..2425
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="color: #05fd14"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    2327..2329
                     /label=V206K
                     /note="color: #ffff00"
     CDS             2447..2611
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /note="color: #cc99b2"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     misc_feature    2640..2867
                     /label=poly A
                     /note="color: #a6acb3"
     misc_feature    3489..3684
                     /label= SV40 promoter-Addgene #11154
                     /note="color: #ffcc00"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aaggcttggt acctgctagc cgttaaaatg gcttctaact ttactcagtt
      961 cgttctcgtc gacaatggcg gaactggcga cgtgactgtc gccccaagca acttcgctaa
     1021 cgggatcgct gaatggatca gctctaactc gcgttcacag gcttacaaag taacctgtag
     1081 cgttcgtcag agctctgcgc agaatcgcaa atacaccatc aaagtcgagg tgcctaaagg
     1141 cgcctggcgt tcgtacttaa atatggaact aaccattcca attttcgcca cgaattccga
     1201 ctgcgagctt attgttaagg caatgcaagg tctcctaaaa gatggaaacc cgattccctc
     1261 agcaatcgca gcaaactccg gcatctacgc catggcttct aactttactc agttcgttct
     1321 cgtcgacaat ggcggaactg gcgacgtgac tgtcgcccca agcaacttcg ctaacgggat
     1381 cgctgaatgg atcagctcta actcgcgttc acaggcttac aaagtaacct gtagcgttcg
     1441 tcagagctct gcgcagaatc gcaaatacac catcaaagtc gaggtgccta aaggcgcctg
     1501 gcgttcgtac ttaaatatgg aactaaccat tccaattttc gccacgaatt ccgactgcga
     1561 gcttattgtt aaggcaatgc aaggtctcct aaaagatgga aacccgattc cctcagcaat
     1621 cgcagcaaac tccggcatct acgcggattc tagaatggtg atgtacagtt caggaggcgg
     1681 cggaagcttg gatccaggtg gaggtggaag cggtagcaaa ggagaagaac ttttcactgg
     1741 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtccg
     1801 tggagagggt gaaggtgatg ctacaaacgg aaaactcacc cttaaattta tttgcactac
     1861 tggaaaacta cctgttccgt ggccaacact tgtcactact ctgacctatg gtgttcaatg
     1921 cttttcccgt tatccggatc acatgaaacg gcatgacttt ttcaagagtg ccatgcccga
     1981 aggttatgta caggaacgca ctatatcttt caaagatgac gggacctaca agacgcgtgc
     2041 tgaagtcaag tttgaaggtg atacccttgt taatcgtatc gagttaaagg gtattgattt
     2101 taaagaagat ggaaacattc ttggacacaa actcgagtac aactttaact cacacaatgt
     2161 atacatcacg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa
     2221 cgttgaagat ggttccgttc aactagcaga ccattatcaa caaaatactc caattggcga
     2281 tggccctgtc cttttaccag acaaccatta cctgtcgaca caatctaagc tttcgaaaga
     2341 tcccaacgaa aagcgtgacc acatggtcct tcttgagttt gtaactgctg ctgggattac
     2401 acatggcatg gatgagctct acaaaggtgg aggtcggacc gaagagtaca agcttatcct
     2461 gaacggtaaa accctgaaag gtgaaaccac caccgaagct gttgacgctg ctaccgcgga
     2521 aaaagttttc aaacagtacg ctaacgacaa cggtgttgac ggtgaatgga cctacgacga
     2581 cgctaccaaa accttcacgg taaccgaata agggcccgtt taaacccgct gatcagcctc
     2641 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     2701 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     2761 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     2821 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     2881 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     2941 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     3001 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3061 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3121 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3181 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3241 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3301 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     3361 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3421 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3481 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3541 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3601 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     3661 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     3721 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     3781 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     3841 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     3901 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     3961 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4021 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     4081 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     4141 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     4201 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     4261 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     4321 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     4381 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     4441 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     4501 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     4561 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     4621 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     4681 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     4741 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4801 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     4861 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4921 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4981 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5101 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5161 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5221 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5281 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5341 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5401 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5461 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5521 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5581 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5641 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5701 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     5761 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5821 gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5881 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5941 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6001 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6061 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6121 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6181 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6241 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6301 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6361 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6421 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6481 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6541 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6601 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6661 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6721 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6781 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6841 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6901 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6961 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7021 cccgaaaagt gccacctgac gtc
//
