LOCUS       Exported               11145 bp DNA     circular SYN 10-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 10, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..1999
                     /label=UBI-pro
                     /note="color: #339966; direction: RIGHT"
     primer_bind     1978..2032
                     /label=cmycNLS-3F
                     /note="color: ºÚÉ«; sequence: 
                     gttgtttggtgttacttctgcaaagcttatgCCAGCCGCCAAGCGCGTCAAGCTG"
     CDS             2009..2035
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /note="color: #993366"
                     /translation="PAAKRVKLD"
     misc_feature    2042..2095
                     /label=bpNLS
                     /note="color: #a6acb3"
     primer_bind     2049..2095
                     /label=bpNLS-1F
                     /note="color: ºÚÉ«; sequence: 
                     CCGCCGACGGATCCGAGTTCGAGAGCCCTAAGAAAAAGAGAAAAGTG"
     CDS             2075..2095
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             2096..6196
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=SpCas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /note="This feature has 3 segments:
                        1: 2096 .. 2119 / #993366
                        2: 2120 .. 2122 / #993366
                        3: 2123 .. 6196 / #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    2120..2122
                     /label=¸ÃÎ»ÖÃÍêºÃ
                     /note="color: #ff0000"
     primer_bind     2733..2770
                     /label=cas9-N394K-F
                     /note="color: ºÚÉ«; sequence: 
                     ctaggctctcgaagtctcggAAGctcgagaacctgatc"
     primer_bind     complement(2733..2770)
                     /label=cas9-R221K-R
                     /note="color: ºÚÉ«; sequence: 
                     gatcaggttctcgagCTTccgagacttcgagagcctag"
     misc_feature    2753..2755
                     /label=R221K
                     /note="color: #ff0000"
     primer_bind     3259..3295
                     /label=cas9-F
                     /note="color: ºÚÉ«; sequence: 
                     gctggtgaagctcaaGcgcgaggacctcctgaggaag"
     primer_bind     complement(3259..3295)
                     /label=cas9-N394K-R
                     /note="color: ºÚÉ«; sequence: 
                     cttcctcaggaggtcctcgcgCttgagcttcaccagc"
     misc_feature    3272..3274
                     /label=N394K
                     /note="color: #ff0000"
     primer_bind     complement(4330..4348)
                     /label=cas9-N394K-R
                     /note="color: ºÚÉ«; sequence: 
                     cttcctcaggaggtcctcgcgCttgagcttcaccagc"
     primer_bind     complement(4596..4634)
                     /label=ADAR-3'-CAS840-1R
                     /note="color: ºÚÉ«; sequence: 
                     tcaggaatgactgtggaacgatGGCatcgacgtcgtaat"
     misc_feature    4610..4612
                     /label=H840A
                     /note="color: #a6acb3"
     primer_bind     4618..4656
                     /label=ADAR-3'-CAS840-2F
                     /note="color: ºÚÉ«; sequence: 
                     tccacagtcattcctgaaggatgactccattgacaacaa"
     misc_feature    6203..6250
                     /label=XTEN
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    6251..6418
                     /label=NC
                     /note="color: #ff00ff"
     primer_bind     6398..6416
                     /label=NC-F-seq
                     /note="color: ºÚÉ«; sequence: AGGCCACAAACATCCCTCC"
     CDS             6419..6439
                     /codon_start=1
                     /label=NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             6446..6541
                     /codon_start=1
                     /label=32aa linker
                     /note="color: #ffcc00"
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     primer_bind     6525..6566
                     /label=LHB-stuI-F
                     /note="color: ºÚÉ«; sequence: 
                     gctcttccggaggatctAGGCCTACCCTCAACATCGAGGATG"
     primer_bind     6526..6563
                     /label=SGGS-MLV-F
                     /note="color: ºÚÉ«; sequence: 
                     ctcttccggaggatctAGGCCTACCCTCAACATCGAGG"
     CDS             6548..8089
                     /codon_start=1
                     /label=M-MLV RT(RNase H del)
                     /note="color: #008000"
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     ADDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPL"
     primer_bind     7210..7239
                     /label=V223A-F
                     /note="color: ºÚÉ«; sequence: GTATgccGATGATCTCCTCCTCGCCGCGAC"
     misc_feature    7214..7216
                     /label=V223A
                     /note="color: #ff0000"
     primer_bind     7824..7843
                     /label=LHB-js-F
                     /note="color: ºÚÉ«; sequence: TCACAATGGGCCAGCCGCTG"
     misc_feature    8102..8155
                     /label=vbpNLS
                     /note="color: #a6acb3"
     primer_bind     8112..8131
                     /label=vbp-J-F
                     /note="color: ºÚÉ«; sequence: CCGATTCCCAACATTCCACC; added: 
                     2022-12-18"
     misc_feature    8135..8155
                     /label=SV40
                     /note="color: #993300; direction: RIGHT"
     CDS             8165..8185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    8245..8421
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(8975..8991)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    8999..9015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(9023..9053)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 9023 .. 9029 / #ffffff / -10
                        2: 9030 .. 9047 / #ffffff
                        3: 9048 .. 9053 / #ffffff / -35"
     protein_bind    9068..9089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(9377..9965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10136..10996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10136 .. 10927 / #ccffcc
                        2: 10928 .. 10996 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10997..11101)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttatgcc agccgccaag cgcgtcaagc tggacggcgg
     2041 aaagcgcacc gccgacggat ccgagttcga gagccctaag aaaaagagaa aagtggacaa
     2101 gaagtactcg atcggcctcg atattgggac taactctgtt ggctgggccg tgatcaccga
     2161 cgagtacaag gtgccctcaa agaagttcaa ggtcctgggc aacaccgatc ggcattccat
     2221 caagaagaat ctcattggcg ctctcctgtt cgacagcggc gagacggctg aggctacgcg
     2281 gctcaagcgc accgcccgca ggcggtacac gcgcaggaag aatcgcatct gctacctgca
     2341 ggagattttc tccaacgaga tggcgaaggt tgacgattct ttcttccaca ggctggagga
     2401 gtcattcctc gtggaggagg ataagaagca cgagcggcat ccaatcttcg gcaacattgt
     2461 cgacgaggtt gcctaccacg agaagtaccc tacgatctac catctgcgga agaagctcgt
     2521 ggactccaca gataaggcgg acctccgcct gatctacctc gctctggccc acatgattaa
     2581 gttcaggggc catttcctga tcgaggggga tctcaacccg gacaatagcg atgttgacaa
     2641 gctgttcatc cagctcgtgc agacgtacaa ccagctcttc gaggagaacc ccattaatgc
     2701 gtcaggcgtc gacgcgaagg ctatcctgtc cgctaggctc tcgaagtctc ggaagctcga
     2761 gaacctgatc gcccagctgc cgggcgagaa gaagaacggc ctgttcggga atctcattgc
     2821 gctcagcctg gggctcacgc ccaacttcaa gtcgaatttc gatctcgctg aggacgccaa
     2881 gctgcagctc tccaaggaca catacgacga tgacctggat aacctcctgg cccagatcgg
     2941 cgatcagtac gcggacctgt tcctcgctgc caagaatctg tcggacgcca tcctcctgtc
     3001 tgatattctc agggtgaaca ccgagattac gaaggctccg ctctcagcct ccatgatcaa
     3061 gcgctacgac gagcaccatc aggatctgac cctcctgaag gcgctggtca ggcagcagct
     3121 ccccgagaag tacaaggaga tcttcttcga tcagtcgaag aacggctacg ctgggtacat
     3181 tgacggcggg gcctctcagg aggagttcta caagttcatc aagccgattc tggagaagat
     3241 ggacggcacg gaggagctgc tggtgaagct caagcgcgag gacctcctga ggaagcagcg
     3301 gacattcgat aacggcagca tcccacacca gattcatctc ggggagctgc acgctatcct
     3361 gaggaggcag gaggacttct accctttcct caaggataac cgcgagaaga tcgagaagat
     3421 tctgactttc aggatcccgt actacgtcgg cccactcgct aggggcaact cccgcttcgc
     3481 ttggatgacc cgcaagtcag aggagacgat cacgccgtgg aacttcgagg aggtggtcga
     3541 caagggcgct agcgctcagt cgttcatcga gaggatgacg aatttcgaca agaacctgcc
     3601 aaatgagaag gtgctcccta agcactcgct cctgtacgag tacttcacag tctacaacga
     3661 gctgactaag gtgaagtatg tgaccgaggg catgaggaag ccggctttcc tgtctgggga
     3721 gcagaagaag gccatcgtgg acctcctgtt caagaccaac cggaaggtca cggttaagca
     3781 gctcaaggag gactacttca agaagattga gtgcttcgat tcggtcgaga tctctggcgt
     3841 tgaggaccgc ttcaacgcct ccctggggac ctaccacgat ctcctgaaga tcattaagga
     3901 taaggacttc ctggacaacg aggagaatga ggatatcctc gaggacattg tgctgacact
     3961 cactctgttc gaggaccggg agatgatcga ggagcgcctg aagacttacg cccatctctt
     4021 cgatgacaag gtcatgaagc agctcaagag gaggaggtac accggctggg ggaggctgag
     4081 caggaagctc atcaacggca ttcgggacaa gcagtccggg aagacgatcc tcgacttcct
     4141 gaagagcgat ggcttcgcga accgcaattt catgcagctg attcacgatg acagcctcac
     4201 attcaaggag gatatccaga aggctcaggt gagcggccag ggggactcgc tgcacgagca
     4261 tatcgcgaac ctcgctggct cgccagctat caagaagggg attctgcaga ccgtgaaggt
     4321 tgtggacgag ctggtgaagg tcatgggcag gcacaagcct gagaacatcg tcattgagat
     4381 ggcccgggag aatcagacca cgcagaaggg ccagaagaac tcacgcgaga ggatgaagag
     4441 gatcgaggag ggcattaagg agctggggtc ccagatcctc aaggagcacc cggtggagaa
     4501 cacgcagctg cagaatgaga agctctacct gtactacctc cagaatggcc gcgatatgta
     4561 tgtggaccag gagctggata ttaacaggct cagcgattac gacgtcgatg ccatcgttcc
     4621 acagtcattc ctgaaggatg actccattga caacaaggtc ctcaccaggt cggacaagaa
     4681 ccggggcaag tctgataatg ttccttcaga ggaggtcgtt aagaagatga agaactactg
     4741 gcgccagctc ctgaatgcca agctgatcac gcagcggaag ttcgataacc tcacaaaggc
     4801 tgagaggggc gggctctctg agctggacaa ggcgggcttc atcaagaggc agctggtcga
     4861 gacacggcag atcactaagc acgttgcgca gattctcgac tcacggatga acactaagta
     4921 cgatgagaat gacaagctga tccgcgaggt gaaggtcatc accctgaagt caaagctcgt
     4981 ctccgacttc aggaaggatt tccagttcta caaggttcgg gagatcaaca attaccacca
     5041 tgcccatgac gcgtacctga acgcggtggt cggcacagct ctgatcaaga agtacccaaa
     5101 gctcgagagc gagttcgtgt acggggacta caaggtttac gatgtgagga agatgatcgc
     5161 caagtcggag caggagattg gcaaggctac cgccaagtac ttcttctact ctaacattat
     5221 gaatttcttc aagacagaga tcactctggc caatggcgag atccggaagc gccccctcat
     5281 cgagacgaac ggcgagacgg gggagatcgt gtgggacaag ggcagggatt tcgcgaccgt
     5341 caggaaggtt ctctccatgc cacaagtgaa tatcgtcaag aagacagagg tccagactgg
     5401 cgggttctct aaggagtcaa ttctgcctaa gcggaacagc gacaagctca tcgcccgcaa
     5461 gaaggactgg gatccgaaga agtacggcgg gttcgacagc cccactgtgg cctactcggt
     5521 cctggttgtg gcgaaggttg agaagggcaa gtccaagaag ctcaagagcg tgaaggagct
     5581 gctggggatc acgattatgg agcgctccag cttcgagaag aacccgatcg atttcctgga
     5641 ggcgaagggc tacaaggagg tgaagaagga cctgatcatt aagctcccca agtactcact
     5701 cttcgagctg gagaacggca ggaagcggat gctggcttcc gctggcgagc tgcagaaggg
     5761 gaacgagctg gctctgccgt ccaagtatgt gaacttcctc tacctggcct cccactacga
     5821 gaagctcaag ggcagccccg aggacaacga gcagaagcag ctgttcgtcg agcagcacaa
     5881 gcattacctc gacgagatca ttgagcagat ttccgagttc tccaagcgcg tgatcctggc
     5941 cgacgcgaat ctggataagg tcctctccgc gtacaacaag caccgcgaca agccaatcag
     6001 ggagcaggct gagaatatca ttcatctctt caccctgacg aacctcggcg cccctgctgc
     6061 tttcaagtac ttcgacacaa ctatcgatcg caagaggtac acaagcacta aggaggtcct
     6121 ggacgcgacc ctcatccacc agtcgattac cggcctctac gagacgcgca tcgacctgtc
     6181 tcagctcggg ggcgacgaat tctccgggag cgagacgcca ggcacctccg agtcggccac
     6241 cccagaatct gccacagtgg tgtccggcca aaagcaggac cgccagggcg gagaacgcag
     6301 aaggtcccag ctcgataggg atcagtgtgc ctactgcaag gagaagggcc actgggccaa
     6361 agactgcccg aaaaagccgc gcggcccacg cggcccaagg ccacaaacat ccctccttcc
     6421 aaagaagaag cggaaggtgg agctcagcgg aggatcttcc ggaggatcta gcggctccga
     6481 gacaccagga acatccgaaa gcgctacacc agaatctagc ggaggctctt ccggaggatc
     6541 taggcctacc ctcaacatcg aggatgagta tcgcctccac gaaacctcca aagaaccgga
     6601 cgtgtccctc ggcagcacat ggctcagcga cttcccacaa gcgtgggccg aaaccggcgg
     6661 catgggcctc gccgtccgcc aagccccact cattatcccg ctgaaggcga cctccacacc
     6721 ggtgtccatc aagcagtacc cgatgagcca agaggcgagg ctcgggatta agccgcacat
     6781 tcagcgcctc ctcgatcaag gcattctcgt gccgtgccaa tccccgtgga atacaccact
     6841 cctcccggtc aaaaagccgg gcaccaacga ctatcgcccg gtccaagatc tccgcgaggt
     6901 caacaagcgc gtggaagaca tccacccgac cgtcccgaac ccgtataatc tgctctccgg
     6961 gctcccacca tcccaccagt ggtatacagt gctggacctc aaagacgcct tcttctgtct
     7021 ccgcctccac ccaacaagcc agccgctctt cgccttcgag tggcgcgacc cggagatggg
     7081 catctccggc caactgacat ggacacgcct cccgcaaggc ttcaagaaca gcccgacact
     7141 cttcaacgag gcgctccata gggacctcgc ggattttcgc atccagcatc cggacctcat
     7201 cctcctccag tatgccgatg atctcctcct cgccgcgacc tccgagctgg attgtcaaca
     7261 aggcacacgc gcgctcctcc aaacactcgg gaacctcggc tatcgcgcgt ccgcgaaaaa
     7321 ggcccaaatc tgccagaagc aagtgaagta cctcgggtat ctgctcaagg aaggccaacg
     7381 ctggctcacc gaagcgcgca aagaaacagt gatggggcaa ccgacaccga aaacaccacg
     7441 ccagctgcgc gagtttctcg gcaaagccgg cttctgtcgc ctcttcatcc cgggctttgc
     7501 cgagatggcc gcgccactct acccactcac caagccgggc acactgttta actgggggcc
     7561 ggatcagcag aaagcctacc aagagatcaa acaagcgctc ctcaccgccc cagcgctcgg
     7621 gctcccagat ctcacaaagc cgttcgagct gttcgtcgat gagaagcaag gctacgcgaa
     7681 gggcgtgctc acacagaagc tcggcccgtg gaggaggcca gtggcctatc tctccaaaaa
     7741 actcgatcca gtggccgccg gctggccacc gtgtctgcgc atggtcgccg cgattgccgt
     7801 gctcacaaag gatgccggca aactcacaat gggccagccg ctggtgatcc tcgcgccaca
     7861 tgccgtggaa gccctcgtca aacagccgcc ggataggtgg ctctccaatg cgcgcatgac
     7921 ccattaccaa gcgctcctcc tcgacaccga tcgcgtccag ttcggcccag tggtcgccct
     7981 caatccggcg acactgctgc cactcccaga ggagggcctc caacacaact gtctggatat
     8041 tctcgcggaa gcgcatggca caaggccaga cctcacagat caaccgctca gcggcggcag
     8101 caaaaggacc gccgattccc aacattccac cccaccaaaa accaagagga aggtgggctc
     8161 tggcccaaag aagaagagga aggtctgagc ggccgcggta cgctgaaatc accagtctct
     8221 ctctacaaat ctatctctct ctattttctc cataaataat gtgtgagtag tttcccgata
     8281 agggaaatta gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag
     8341 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
     8401 tccagtacta aaatccagat ctcctaaagt ccctatagat ctttgtcgtg aatataaacc
     8461 agacacgaga cgactaaacc tggagcccag acgccgttcg aagctagaag taccgcttag
     8521 gcaggaggcc gttagggaaa agatgctaag gcagggttgg ttacgttgac tcccccgtag
     8581 gtttggttta aatatgatga agtggacgga aggaaggagg aagacaagga aggataaggt
     8641 tgcaggccct gtgcaaggta agaagatgga aatttgatag aggtacgcta ctatacttat
     8701 actatacgct aagggaatgc ttgtatttat accctatacc ccctaataac cccttatcaa
     8761 tttaagaaat aatccgcata agcccccgct taaaaattgg tatcagagcc atgaataggt
     8821 ctatgaccaa aactcaagag gataaaacct caccaaaata cgaaagagtt cttaactcta
     8881 aagataaaag atctttcaag atcaaaacta gttccctcac accggtgacg gggatcgcat
     8941 gcgatatctc gagatctagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     9001 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9061 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9121 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9181 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9241 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9301 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9361 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9421 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9481 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9541 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9601 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9661 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9721 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9781 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9841 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9901 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9961 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10021 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10081 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10141 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10201 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10261 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10321 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10381 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10441 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10501 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10561 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10621 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10681 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10741 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10801 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10861 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10921 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10981 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11041 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11101 gcacatttcc ccgaaaagtg ccacctgcca gtgccaagct aattc
//
