LOCUS       Exported               11775 bp DNA     circular SYN 10-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 10, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..1999
                     /label=UBI-pro
                     /note="color: #339966; direction: RIGHT"
     CDS             2009..2569
                     /codon_start=1
                     /label=Csy4
                     /note="color: #33cccc"
                     /translation="MDHYLDIRLRPDPEFPPAQLMSVLFGKLHQALVAQGGDRIGVSFP
                     DLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQ
                     AKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGP
                     LQVTAEEGGFTCYGLSKGGFVPWF"
     misc_feature    2570..2578
                     /label=GSG linker
                     /note="color: #a6acb3"
     CDS             2579..2635
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 2632"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2639..2665
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /note="color: #993366"
                     /translation="PAAKRVKLD"
     misc_feature    2672..2725
                     /label=bpNLS
                     /note="color: #a6acb3"
     primer_bind     2679..2725
                     /label=bpNLS-1F
                     /note="color: ºÚÉ«; sequence: 
                     CCGCCGACGGATCCGAGTTCGAGAGCCCTAAGAAAAAGAGAAAAGTG"
     CDS             2705..2725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             2726..6826
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=SpCas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /note="This feature has 3 segments:
                        1: 2726 .. 2749 / #993366
                        2: 2750 .. 2752 / #993366
                        3: 2753 .. 6826 / #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    2750..2752
                     /label=¸ÃÎ»ÖÃÍêºÃ
                     /note="color: #ff0000"
     primer_bind     3363..3400
                     /label=cas9-N394K-F
                     /note="color: ºÚÉ«; sequence: 
                     ctaggctctcgaagtctcggAAGctcgagaacctgatc"
     primer_bind     complement(3363..3400)
                     /label=cas9-R221K-R
                     /note="color: ºÚÉ«; sequence: 
                     gatcaggttctcgagCTTccgagacttcgagagcctag"
     misc_feature    3383..3385
                     /label=R221K
                     /note="color: #ff0000"
     primer_bind     3889..3925
                     /label=cas9-F
                     /note="color: ºÚÉ«; sequence: 
                     gctggtgaagctcaaGcgcgaggacctcctgaggaag"
     primer_bind     complement(3889..3925)
                     /label=cas9-N394K-R
                     /note="color: ºÚÉ«; sequence: 
                     cttcctcaggaggtcctcgcgCttgagcttcaccagc"
     misc_feature    3902..3904
                     /label=N394K
                     /note="color: #ff0000"
     primer_bind     complement(4960..4978)
                     /label=cas9-N394K-R
                     /note="color: ºÚÉ«; sequence: 
                     cttcctcaggaggtcctcgcgCttgagcttcaccagc"
     primer_bind     complement(5226..5264)
                     /label=ADAR-3'-CAS840-1R
                     /note="color: ºÚÉ«; sequence: 
                     tcaggaatgactgtggaacgatGGCatcgacgtcgtaat"
     misc_feature    5240..5242
                     /label=H840A
                     /note="color: #a6acb3"
     primer_bind     5248..5286
                     /label=ADAR-3'-CAS840-2F
                     /note="color: ºÚÉ«; sequence: 
                     tccacagtcattcctgaaggatgactccattgacaacaa"
     misc_feature    6833..6880
                     /label=XTEN
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    6881..7048
                     /label=NC
                     /note="color: #ff00ff"
     primer_bind     7028..7046
                     /label=NC-F-seq
                     /note="color: ºÚÉ«; sequence: AGGCCACAAACATCCCTCC"
     CDS             7049..7069
                     /codon_start=1
                     /label=NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             7076..7171
                     /codon_start=1
                     /label=32aa linker
                     /note="color: #ffcc00"
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     primer_bind     7155..7196
                     /label=LHB-stuI-F
                     /note="color: ºÚÉ«; sequence: 
                     gctcttccggaggatctAGGCCTACCCTCAACATCGAGGATG"
     primer_bind     7156..7193
                     /label=SGGS-MLV-F
                     /note="color: ºÚÉ«; sequence: 
                     ctcttccggaggatctAGGCCTACCCTCAACATCGAGG"
     CDS             7178..8719
                     /codon_start=1
                     /label=M-MLV RT(RNase H del)
                     /note="color: #008000"
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     ADDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPL"
     primer_bind     7840..7869
                     /label=V223A-F
                     /note="color: ºÚÉ«; sequence: GTATgccGATGATCTCCTCCTCGCCGCGAC"
     misc_feature    7844..7846
                     /label=V223A
                     /note="color: #ff0000"
     primer_bind     8454..8473
                     /label=LHB-js-F
                     /note="color: ºÚÉ«; sequence: TCACAATGGGCCAGCCGCTG"
     misc_feature    8732..8785
                     /label=vbpNLS
                     /note="color: #a6acb3"
     primer_bind     8742..8761
                     /label=vbp-J-F
                     /note="color: ºÚÉ«; sequence: CCGATTCCCAACATTCCACC; added: 
                     2022-12-18"
     misc_feature    8765..8785
                     /label=SV40
                     /note="color: #993300; direction: RIGHT"
     CDS             8795..8815
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    8875..9051
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(9605..9621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    9629..9645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(9653..9683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 9653 .. 9659 / #ffffff / -10
                        2: 9660 .. 9677 / #ffffff
                        3: 9678 .. 9683 / #ffffff / -35"
     protein_bind    9698..9719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(10007..10595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10766..11626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10766 .. 11557 / #ccffcc
                        2: 11558 .. 11626 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11627..11731)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttatgat ggaccactac ctcgacatca ggctcaggcc
     2041 agacccagag ttcccaccag cccagctcat gtccgtcctc ttcggcaagc tccaccaggc
     2101 cctcgtggcc cagggcggcg acaggatcgg cgtgtccttc ccagacctcg acgagtccag
     2161 gtccaggctc ggcgagaggc tccgcatcca cgcctccgcc gacgacctca gggccctcct
     2221 cgccaggccg tggctggagg gcctcaggga ccacctccag ttcggcgagc cagccgtggt
     2281 gccacaccca accccataca ggcaagtgtc cagggtgcaa gccaagtcca acccagagag
     2341 gctcaggagg aggctcatga ggaggcacga cctctccgag gaagaggcca ggaagcgcat
     2401 cccagacacc gtggccaggg ccctcgacct cccattcgtg accctcaggt cccagtccac
     2461 cggccagcac ttccgcctct tcatcaggca cggcccactc caggtgaccg ccgaggaggg
     2521 cggctttacc tgctacggcc tctccaaggg cggcttcgtg ccgtggttcg gctccggcgc
     2581 caccaacttc tccctcctca agcaagccgg cgacgtggag gagaacccag gcccaatgcc
     2641 agccgccaag cgcgtcaagc tggacggcgg aaagcgcacc gccgacggat ccgagttcga
     2701 gagccctaag aaaaagagaa aagtggacaa gaagtactcg atcggcctcg atattgggac
     2761 taactctgtt ggctgggccg tgatcaccga cgagtacaag gtgccctcaa agaagttcaa
     2821 ggtcctgggc aacaccgatc ggcattccat caagaagaat ctcattggcg ctctcctgtt
     2881 cgacagcggc gagacggctg aggctacgcg gctcaagcgc accgcccgca ggcggtacac
     2941 gcgcaggaag aatcgcatct gctacctgca ggagattttc tccaacgaga tggcgaaggt
     3001 tgacgattct ttcttccaca ggctggagga gtcattcctc gtggaggagg ataagaagca
     3061 cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt gcctaccacg agaagtaccc
     3121 tacgatctac catctgcgga agaagctcgt ggactccaca gataaggcgg acctccgcct
     3181 gatctacctc gctctggccc acatgattaa gttcaggggc catttcctga tcgaggggga
     3241 tctcaacccg gacaatagcg atgttgacaa gctgttcatc cagctcgtgc agacgtacaa
     3301 ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc gacgcgaagg ctatcctgtc
     3361 cgctaggctc tcgaagtctc ggaagctcga gaacctgatc gcccagctgc cgggcgagaa
     3421 gaagaacggc ctgttcggga atctcattgc gctcagcctg gggctcacgc ccaacttcaa
     3481 gtcgaatttc gatctcgctg aggacgccaa gctgcagctc tccaaggaca catacgacga
     3541 tgacctggat aacctcctgg cccagatcgg cgatcagtac gcggacctgt tcctcgctgc
     3601 caagaatctg tcggacgcca tcctcctgtc tgatattctc agggtgaaca ccgagattac
     3661 gaaggctccg ctctcagcct ccatgatcaa gcgctacgac gagcaccatc aggatctgac
     3721 cctcctgaag gcgctggtca ggcagcagct ccccgagaag tacaaggaga tcttcttcga
     3781 tcagtcgaag aacggctacg ctgggtacat tgacggcggg gcctctcagg aggagttcta
     3841 caagttcatc aagccgattc tggagaagat ggacggcacg gaggagctgc tggtgaagct
     3901 caagcgcgag gacctcctga ggaagcagcg gacattcgat aacggcagca tcccacacca
     3961 gattcatctc ggggagctgc acgctatcct gaggaggcag gaggacttct accctttcct
     4021 caaggataac cgcgagaaga tcgagaagat tctgactttc aggatcccgt actacgtcgg
     4081 cccactcgct aggggcaact cccgcttcgc ttggatgacc cgcaagtcag aggagacgat
     4141 cacgccgtgg aacttcgagg aggtggtcga caagggcgct agcgctcagt cgttcatcga
     4201 gaggatgacg aatttcgaca agaacctgcc aaatgagaag gtgctcccta agcactcgct
     4261 cctgtacgag tacttcacag tctacaacga gctgactaag gtgaagtatg tgaccgaggg
     4321 catgaggaag ccggctttcc tgtctgggga gcagaagaag gccatcgtgg acctcctgtt
     4381 caagaccaac cggaaggtca cggttaagca gctcaaggag gactacttca agaagattga
     4441 gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc ttcaacgcct ccctggggac
     4501 ctaccacgat ctcctgaaga tcattaagga taaggacttc ctggacaacg aggagaatga
     4561 ggatatcctc gaggacattg tgctgacact cactctgttc gaggaccggg agatgatcga
     4621 ggagcgcctg aagacttacg cccatctctt cgatgacaag gtcatgaagc agctcaagag
     4681 gaggaggtac accggctggg ggaggctgag caggaagctc atcaacggca ttcgggacaa
     4741 gcagtccggg aagacgatcc tcgacttcct gaagagcgat ggcttcgcga accgcaattt
     4801 catgcagctg attcacgatg acagcctcac attcaaggag gatatccaga aggctcaggt
     4861 gagcggccag ggggactcgc tgcacgagca tatcgcgaac ctcgctggct cgccagctat
     4921 caagaagggg attctgcaga ccgtgaaggt tgtggacgag ctggtgaagg tcatgggcag
     4981 gcacaagcct gagaacatcg tcattgagat ggcccgggag aatcagacca cgcagaaggg
     5041 ccagaagaac tcacgcgaga ggatgaagag gatcgaggag ggcattaagg agctggggtc
     5101 ccagatcctc aaggagcacc cggtggagaa cacgcagctg cagaatgaga agctctacct
     5161 gtactacctc cagaatggcc gcgatatgta tgtggaccag gagctggata ttaacaggct
     5221 cagcgattac gacgtcgatg ccatcgttcc acagtcattc ctgaaggatg actccattga
     5281 caacaaggtc ctcaccaggt cggacaagaa ccggggcaag tctgataatg ttccttcaga
     5341 ggaggtcgtt aagaagatga agaactactg gcgccagctc ctgaatgcca agctgatcac
     5401 gcagcggaag ttcgataacc tcacaaaggc tgagaggggc gggctctctg agctggacaa
     5461 ggcgggcttc atcaagaggc agctggtcga gacacggcag atcactaagc acgttgcgca
     5521 gattctcgac tcacggatga acactaagta cgatgagaat gacaagctga tccgcgaggt
     5581 gaaggtcatc accctgaagt caaagctcgt ctccgacttc aggaaggatt tccagttcta
     5641 caaggttcgg gagatcaaca attaccacca tgcccatgac gcgtacctga acgcggtggt
     5701 cggcacagct ctgatcaaga agtacccaaa gctcgagagc gagttcgtgt acggggacta
     5761 caaggtttac gatgtgagga agatgatcgc caagtcggag caggagattg gcaaggctac
     5821 cgccaagtac ttcttctact ctaacattat gaatttcttc aagacagaga tcactctggc
     5881 caatggcgag atccggaagc gccccctcat cgagacgaac ggcgagacgg gggagatcgt
     5941 gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt ctctccatgc cacaagtgaa
     6001 tatcgtcaag aagacagagg tccagactgg cgggttctct aaggagtcaa ttctgcctaa
     6061 gcggaacagc gacaagctca tcgcccgcaa gaaggactgg gatccgaaga agtacggcgg
     6121 gttcgacagc cccactgtgg cctactcggt cctggttgtg gcgaaggttg agaagggcaa
     6181 gtccaagaag ctcaagagcg tgaaggagct gctggggatc acgattatgg agcgctccag
     6241 cttcgagaag aacccgatcg atttcctgga ggcgaagggc tacaaggagg tgaagaagga
     6301 cctgatcatt aagctcccca agtactcact cttcgagctg gagaacggca ggaagcggat
     6361 gctggcttcc gctggcgagc tgcagaaggg gaacgagctg gctctgccgt ccaagtatgt
     6421 gaacttcctc tacctggcct cccactacga gaagctcaag ggcagccccg aggacaacga
     6481 gcagaagcag ctgttcgtcg agcagcacaa gcattacctc gacgagatca ttgagcagat
     6541 ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat ctggataagg tcctctccgc
     6601 gtacaacaag caccgcgaca agccaatcag ggagcaggct gagaatatca ttcatctctt
     6661 caccctgacg aacctcggcg cccctgctgc tttcaagtac ttcgacacaa ctatcgatcg
     6721 caagaggtac acaagcacta aggaggtcct ggacgcgacc ctcatccacc agtcgattac
     6781 cggcctctac gagacgcgca tcgacctgtc tcagctcggg ggcgacgaat tctccgggag
     6841 cgagacgcca ggcacctccg agtcggccac cccagaatct gccacagtgg tgtccggcca
     6901 aaagcaggac cgccagggcg gagaacgcag aaggtcccag ctcgataggg atcagtgtgc
     6961 ctactgcaag gagaagggcc actgggccaa agactgcccg aaaaagccgc gcggcccacg
     7021 cggcccaagg ccacaaacat ccctccttcc aaagaagaag cggaaggtgg agctcagcgg
     7081 aggatcttcc ggaggatcta gcggctccga gacaccagga acatccgaaa gcgctacacc
     7141 agaatctagc ggaggctctt ccggaggatc taggcctacc ctcaacatcg aggatgagta
     7201 tcgcctccac gaaacctcca aagaaccgga cgtgtccctc ggcagcacat ggctcagcga
     7261 cttcccacaa gcgtgggccg aaaccggcgg catgggcctc gccgtccgcc aagccccact
     7321 cattatcccg ctgaaggcga cctccacacc ggtgtccatc aagcagtacc cgatgagcca
     7381 agaggcgagg ctcgggatta agccgcacat tcagcgcctc ctcgatcaag gcattctcgt
     7441 gccgtgccaa tccccgtgga atacaccact cctcccggtc aaaaagccgg gcaccaacga
     7501 ctatcgcccg gtccaagatc tccgcgaggt caacaagcgc gtggaagaca tccacccgac
     7561 cgtcccgaac ccgtataatc tgctctccgg gctcccacca tcccaccagt ggtatacagt
     7621 gctggacctc aaagacgcct tcttctgtct ccgcctccac ccaacaagcc agccgctctt
     7681 cgccttcgag tggcgcgacc cggagatggg catctccggc caactgacat ggacacgcct
     7741 cccgcaaggc ttcaagaaca gcccgacact cttcaacgag gcgctccata gggacctcgc
     7801 ggattttcgc atccagcatc cggacctcat cctcctccag tatgccgatg atctcctcct
     7861 cgccgcgacc tccgagctgg attgtcaaca aggcacacgc gcgctcctcc aaacactcgg
     7921 gaacctcggc tatcgcgcgt ccgcgaaaaa ggcccaaatc tgccagaagc aagtgaagta
     7981 cctcgggtat ctgctcaagg aaggccaacg ctggctcacc gaagcgcgca aagaaacagt
     8041 gatggggcaa ccgacaccga aaacaccacg ccagctgcgc gagtttctcg gcaaagccgg
     8101 cttctgtcgc ctcttcatcc cgggctttgc cgagatggcc gcgccactct acccactcac
     8161 caagccgggc acactgttta actgggggcc ggatcagcag aaagcctacc aagagatcaa
     8221 acaagcgctc ctcaccgccc cagcgctcgg gctcccagat ctcacaaagc cgttcgagct
     8281 gttcgtcgat gagaagcaag gctacgcgaa gggcgtgctc acacagaagc tcggcccgtg
     8341 gaggaggcca gtggcctatc tctccaaaaa actcgatcca gtggccgccg gctggccacc
     8401 gtgtctgcgc atggtcgccg cgattgccgt gctcacaaag gatgccggca aactcacaat
     8461 gggccagccg ctggtgatcc tcgcgccaca tgccgtggaa gccctcgtca aacagccgcc
     8521 ggataggtgg ctctccaatg cgcgcatgac ccattaccaa gcgctcctcc tcgacaccga
     8581 tcgcgtccag ttcggcccag tggtcgccct caatccggcg acactgctgc cactcccaga
     8641 ggagggcctc caacacaact gtctggatat tctcgcggaa gcgcatggca caaggccaga
     8701 cctcacagat caaccgctca gcggcggcag caaaaggacc gccgattccc aacattccac
     8761 cccaccaaaa accaagagga aggtgggctc tggcccaaag aagaagagga aggtctgagc
     8821 ggccgcggta cgctgaaatc accagtctct ctctacaaat ctatctctct ctattttctc
     8881 cataaataat gtgtgagtag tttcccgata agggaaatta gggttcttat agggtttcgc
     8941 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     9001 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat ctcctaaagt
     9061 ccctatagat ctttgtcgtg aatataaacc agacacgaga cgactaaacc tggagcccag
     9121 acgccgttcg aagctagaag taccgcttag gcaggaggcc gttagggaaa agatgctaag
     9181 gcagggttgg ttacgttgac tcccccgtag gtttggttta aatatgatga agtggacgga
     9241 aggaaggagg aagacaagga aggataaggt tgcaggccct gtgcaaggta agaagatgga
     9301 aatttgatag aggtacgcta ctatacttat actatacgct aagggaatgc ttgtatttat
     9361 accctatacc ccctaataac cccttatcaa tttaagaaat aatccgcata agcccccgct
     9421 taaaaattgg tatcagagcc atgaataggt ctatgaccaa aactcaagag gataaaacct
     9481 caccaaaata cgaaagagtt cttaactcta aagataaaag atctttcaag atcaaaacta
     9541 gttccctcac accggtgacg gggatcgcat gcgatatctc gagatctagc ttggcgtaat
     9601 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9661 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9721 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9781 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9841 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9901 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9961 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    10021 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    10081 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10141 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10201 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10261 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10321 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10381 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10441 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10501 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10561 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10621 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10681 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10741 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10801 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10861 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10921 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10981 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    11041 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    11101 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    11161 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    11221 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    11281 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    11341 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    11401 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    11461 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11521 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11581 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11641 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11701 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcca
    11761 gtgccaagct aattc
//
