LOCUS       Exported                5630 bp ds-DNA     circular SYN 13-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Broad Institute
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 30, 2023 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    531..553
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(542..562)
                     /label=Vector.FOR
     primer_bind     complement(542..562)
                     /label=Vector.FOR
     primer_bind     complement(542..559)
                     /label=Vector.FOR
     misc_feature    560..1559
                     /label=F1
     primer_bind     complement(1258..1271)
                     /label=m83_r
     primer_bind     complement(1414..1438)
                     /label=m49_R
     misc_feature    1419..1473
                     /label=m51_GA_fragment
     primer_bind     complement(1444..1465)
                     /label=m58_r
     CDS             1445..1447
                     /codon_start=1
                     /label=m51 (T296)
                     /translation="T"
     misc_feature    1450..1502
                     /label=m61_GA_fragment
     misc_feature    1450..1502
                     /label=m60_GA_fragment
     misc_feature    1450..1502
                     /label=m59_GA_fragment
     primer_bind     1454..1476
                     /label=m53_f
     CDS             1469..1471
                     /codon_start=1
                     /label=m59 (M304)
                     /translation="M"
     CDS             1475..1477
                     /codon_start=1
                     /label=m60 (V306)
                     /translation="V"
     CDS             1478..1480
                     /codon_start=1
                     /label=m61 (C307)
                     /translation="C"
     primer_bind     complement(1484..1504)
                     /label=m67_r
     primer_bind     1487..1506
                     /label=m62_f
     misc_feature    1492..1546
                     /label=m69_GA_fragment
     misc_feature    1492..1546
                     /label=m68_GA_fragment
     misc_feature    1508..1564
                     /label=m74_GA_fragment
     misc_feature    1508..1564
                     /label=m73_GA_fragment
     misc_feature    1508..1564
                     /label=m71_GA_fragment
     primer_bind     complement(1508..1528)
                     /label=m70_r
     CDS             1520..1522
                     /codon_start=1
                     /label=m68 (H321)
                     /translation="H"
     CDS             1523..1525
                     /codon_start=1
                     /label=m69 (F322)
                     /translation="F"
     primer_bind     1532..1552
                     /label=m70_f
     CDS             1532..1534
                     /codon_start=1
                     /label=m71 (S325)
                     /translation="S"
     CDS             1538..1540
                     /codon_start=1
                     /label=m73 (T327)
                     /translation="T"
     CDS             1541..1543
                     /codon_start=1
                     /label=m74 (T328)
                     /translation="T"
     primer_bind     1547..1564
                     /label=m75_f
     primer_bind     complement(1548..1564)
                     /label=m82_r
     misc_feature    1549..1600
                     /label=m85_GA_fragment
     primer_bind     complement(1549..1567)
                     /label=m83_r
     misc_feature    1560..2473
                     /label=F2
     CDS             1574..1576
                     /codon_start=1
                     /label=m85 (H339)
                     /translation="H"
     CDS             1574..1576
                     /codon_start=1
                     /label=m85 (H339)
                     /translation="H"
     primer_bind     1580..1600
                     /label=m86_f
     misc_feature    1874..1927
                     /label=m91_GA_fragment
     primer_bind     complement(1874..1897)
                     /label=m90_r
     primer_bind     complement(1893..1915)
                     /label=m95_r
     misc_feature    1899..1947
                     /label=m97_GA_fragment
     misc_feature    1899..1947
                     /label=m96_GA_fragment
     CDS             1901..1903
                     /codon_start=1
                     /label=m91 (T448)
                     /translation="T"
     primer_bind     1910..1927
                     /label=m92_f
     primer_bind     complement(1915..1933)
                     /label=m99_r
     misc_feature    1919..1975
                     /label=m104_GA_fragment
     misc_feature    1919..1975
                     /label=m103_GA_fragment
     misc_feature    1919..1975
                     /label=m100_GA_fragment
     CDS             1925..1927
                     /codon_start=1
                     /label=m96 (A456)
                     /translation="A"
     CDS             1928..1930
                     /codon_start=1
                     /label=m97 (D457)
                     /translation="D"
     primer_bind     1931..1952
                     /label=m97_f
     CDS             1943..1945
                     /codon_start=1
                     /label=m100 (K462)
                     /translation="K"
     CDS             1952..1954
                     /codon_start=1
                     /label=m103 (Y465)
                     /translation="Y"
     CDS             1955..1957
                     /codon_start=1
                     /label=m104 (S466)
                     /translation="S"
     primer_bind     1961..1977
                     /label=m105_f
     misc_feature    1987..2038
                     /label=m121_GA_fragment
     primer_bind     complement(1987..2008)
                     /label=m120_r
     misc_feature    2000..2057
                     /label=m127_GA_fragment
     misc_feature    2000..2057
                     /label=m125_GA_fragment
     primer_bind     complement(2000..2020)
                     /label=m124_r
     CDS             2012..2014
                     /codon_start=1
                     /label=m121 (E485)
                     /translation="E"
     primer_bind     2018..2038
                     /label=m122_f
     CDS             2024..2026
                     /codon_start=1
                     /label=m125 (I489)
                     /translation="I"
     misc_feature    2026..2077
                     /label=m133_GA_fragment
     primer_bind     complement(2026..2047)
                     /label=m132_r
     CDS             2030..2032
                     /codon_start=1
                     /label=m127 (N491)
                     /translation="N"
     primer_bind     2036..2057
                     /label=m128_f
     CDS             2051..2053
                     /codon_start=1
                     /label=m133 (E498)
                     /translation="E"
     primer_bind     2057..2077
                     /label=m133_GA_f
     primer_bind     complement(2452..2473)
                     /label=pMSSM24_rev
     misc_feature    2474..2496
                     /label=ATGAGCCAGATCACCATCCAGGCCAGACTGATCAGCTTCGAGAGCAA
                     /label=ATGAGCCAGATCACCATCCAGGCCAGACTGATCAGCTTCGAGAGCAACAG 
                     ACAGCAGCTGTGGAAGCTGATGGCCGACCTGAACACCCCTCTGATCAACGAGCTGCTG 
                     TGTCAGCTGGGACAGCACCCCGATTTCGAGAAGTGGCAGCAGAAGGGCAAGCTGCCTA 
                     GCACCGTGGTGTCTCAGCTGTGCCAGCCTCTGAAAACAGACCCCAGATTTGCCGGCCA 
                     GCCTAGCAGACTGTACATGAGCGCCATCCACATCGTGGACTACATCTACAAGAGCTGG 
                     CTGGCCATCCAGAAGCGGCTGCAGCAACAGCTGGATGGCAAGACCAGATGGCTGGAAA 
                     TGCTGAACAGCGACGCCGAGCTGGTGGAACTGTCTGGCGATACACTGGAAGCCATCAG 
                     AGTGAAGGCCGCCGAGATCCTGGCCATTGCTATGCCTGCCAGCGAGAGCGATAGCGCT 
                     AGCCCCAAAGGCAAAAAGGGCAAGAAAGAGAAGAAGCCCTCCAGCAGCAGCCCCAAGA 
                     GAAGCCTGAGCAAGACCCTGTTCGACGCCTACCAAGAGACAGAGGACATCAAGAGCAG 
                     AAGCGCCATCTCCTACCTGCTGAAGAACGGCTGCAAGCTGACCGACAAAGAAGAGGAC 
                     AGCGAGAAGTTCGCCAAGCGGCGGAGACAGGTGGAAATCCAGATCCAGAGACTGACCG 
                     AGAAGCTGATTAGCAGAATGCCCAAGGGCCGCGACCTGACCAATGCCAAGTGGCTCGA 
                     AACACTGCTGACCGCCACAACCACAGTGGCCGAGGATAATGCCCAGGCCAAGAGATGG 
                     CAGGACATCCTGCTGACCAGAAGCAGCTCCCTGCCTTTTCCACTGGTGTTCGAGACAA 
                     ACGAGGACATGGTCTGGTCCAAGAACCAGAAAGGCCGGCTGTGCGTGCACTTCAACGG 
                     CCTGAGCGATCTGATCTTCGAGGTGTACTGCGGCAACCGGCAGCTGCACTGGTTCCAG 
                     CGGTTTCTGGAAGATCAGCAGACCAAGCGCAAGAGCAAGAATCAGCACAGCAGCGGCC 
                     TGTTCACCCTGAGAAATGGACACCTCGTGTGGCTGGAAGGCGAAGGCAAAGGCGAGCC 
                     TTGGAACCTGCACCACCTGACACTGTACTGCTGCGTGGACAACCGGCTGTGGACAGAA 
                     GAGGGCACAGAGATCGTGCGGCAAGAGAAGGCCGATGAGATCACCAAGTTCATCACCA 
                     ACATGAAGAAGAAGTCCGACCTGAGCGACACCCAGCAGGCCCTGATTCAGAGAAAGCA 
                     GAGCACCCTGACCAGGATCAACAACTCCTTCGAGAGGCCCAGCCAGCCACTGTATCAG 
                     GGCCAGTCTCATATCCTCGTGGGCGTGTCACTGGGCCTCGAGAAACCTGCTACAGTCG 
                     CCGTGGTGGACGCCATTGCCAACAAGGTTCTGGCCTACCGGTCCATCAAACAGCTGCT 
                     CGGCGACAACTATGAGCTGCTGAACCGTCAGCGACGGCAGCAGCAGTATCTGAGCCAC 
                     GAGAGACACAAGGCCCAGAAGAACTTCAGCCCCAACCAGTTTGGCGCCTCTGAACTGG 
                     GCCAGCACATCGATAGACTGCTGGCCAAGGCCATTGTGGCCCTGGCCAGAACATACAA 
                     GGCCGGCAGTATCGTGCTGCCCAAGCTGGGCGATATGAGAGAAGTGGTGCAGAGCGAG 
                     ATCCAGGCTATCGCCGAACAGAAGTTCCCCGGCTATATCGAGGGCCAGCAGAAGTATG 
                     CCAAGCAGTATAGAGTGAACGTGCACCGGTGGTCCTACGGCAGGCTGATCCAGAGCAT 
                     CCAGTCTAAGGCTGCCCAGACCGGCATCGTGATCGAGGAAGGCAAGCAGCCCATTAGA 
                     GGCAGCCCTCACGACAAGGCTAAAGAACTGGCTCTGAGCGCCTACAACCTGAGGCTGA 
                     CAAGAAGAAGCTAA
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=ATGAGCCAGATCACCATCCAGGCCAGACTGATCAGC
                     TTCGA 
                     GAGCAACAGACAGCAGCTGTGGAAGCTGATGGCCGACCTGAACACCCCTCTGATCAAC 
                     GAGCTGCTGTGTCAGCTGGGACAGCACCCCGATTTCGAGAAGTGGCAGCAGAAGGGCA 
                     AGCTGCCTAGCACCGTGGTGTCTCAGCTGTGCCAGCCTCTGAAAACAGACCCCAGATT 
                     TGCCGGCCAGCCTAGCAGACTGTACATGAGCGCCATCCACATCGTGGACTACATCTAC 
                     AAGAGCTGGCTGGCCATCCAGAAGCGGCTGCAGCAACAGCTGGATGGCAAGACCAGAT 
                     GGCTGGAAATGCTGAACAGCGACGCCGAGCTGGTGGAACTGTCTGGCGATACACTGGA 
                     AGCCATCAGAGTGAAGGCCGCCGAGATCCTGGCCATTGCTATGCCTGCCAGCGAGAGC 
                     GATAGCGCTAGCCCCAAAGGCAAAAAGGGCAAGAAAGAGAAGAAGCCCTCCAGCAGCA 
                     GCCCCAAGAGAAGCCTGAGCAAGACCCTGTTCGACGCCTACCAAGAGACAGAGGACAT 
                     CAAGAGCAGAAGCGCCATCTCCTACCTGCTGAAGAACGGCTGCAAGCTGACCGACAAA 
                     GAAGAGGACAGCGAGAAGTTCGCCAAGCGGCGGAGACAGGTGGAAATCCAGATCCAGA 
                     GACTGACCGAGAAGCTGATTAGCAGAATGCCCAAGGGCCGCGACCTGACCAATGCCAA 
                     GTGGCTCGAAACACTGCTGACCGCCACAACCACAGTGGCCGAGGATAATGCCCAGGCC 
                     AAGAGATGGCAGGACATCCTGCTGACCAGAAGCAGCTCCCTGCCTTTTCCACTGGTGT 
                     TCGAGACAAACGAGGACATGGTCTGGTCCAAGAACCAGAAAGGCCGGCTGTGCGTGCA 
                     CTTCAACGGCCTGAGCGATCTGATCTTCGAGGTGTACTGCGGCAACCGGCAGCTGCAC 
                     TGGTTCCAGCGGTTTCTGGAAGATCAGCAGACCAAGCGCAAGAGCAAGAATCAGCACA 
                     GCAGCGGCCTGTTCACCCTGAGAAATGGACACCTCGTGTGGCTGGAAGGCGAAGGCAA 
                     AGGCGAGCCTTGGAACCTGCACCACCTGACACTGTACTGCTGCGTGGACAACCGGCTG 
                     TGGACAGAAGAGGGCACAGAGATCGTGCGGCAAGAGAAGGCCGATGAGATCACCAAGT 
                     TCATCACCAACATGAAGAAGAAGTCCGACCTGAGCGACACCCAGCAGGCCCTGATTCA 
                     GAGAAAGCAGAGCACCCTGACCAGGATCAACAACTCCTTCGAGAGGCCCAGCCAGCCA 
                     CTGTATCAGGGCCAGTCTCATATCCTCGTGGGCGTGTCACTGGGCCTCGAGAAACCTG 
                     CTACAGTCGCCGTGGTGGACGCCATTGCCAACAAGGTTCTGGCCTACCGGTCCATCAA 
                     ACAGCTGCTCGGCGACAACTATGAGCTGCTGAACCGTCAGCGACGGCAGCAGCAGTAT 
                     CTGAGCCACGAGAGACACAAGGCCCAGAAGAACTTCAGCCCCAACCAGTTTGGCGCCT 
                     CTGAACTGGGCCAGCACATCGATAGACTGCTGGCCAAGGCCATTGTGGCCCTGGCCAG 
                     AACATACAAGGCCGGCAGTATCGTGCTGCCCAAGCTGGGCGATATGAGAGAAGTGGTG 
                     CAGAGCGAGATCCAGGCTATCGCCGAACAGAAGTTCCCCGGCTATATCGAGGGCCAGC 
                     AGAAGTATGCCAAGCAGTATAGAGTGAACGTGCACCGGTGGTCCTACGGCAGGCTGAT 
                     CCAGAGCATCCAGTCTAAGGCTGCCCAGACCGGCATCGTGATCGAGGAAGGCAAGCAG 
                     CCCATTAGAGGCAGCCCTCACGACAAGGCTAAAGAACTGGCTCTGAGCGCCTACAACC 
                     TGAGGCTGACAAGAAGAAGCTAA"
     primer_bind     2474..2493
                     /label=Vector.FOR
     primer_bind     complement(2475..2521)
                     /label=Fragment.REV
     misc_feature    2478..2496
                     /label=T7 Terminator
                     /note="/created_by=zhangf"
     primer_bind     complement(2576..2595)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_site      2641..2696
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(2695..2714)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3057..3073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3057..3073)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3070..3092)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3081..3097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3105..3135)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3150..3171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3230..3425
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3276..3411
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3338..3357
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(3361..3372)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    3431..3565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3468..3487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3522..3541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3633..3650)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3804..4392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3884..3903)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4563..5423)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5186..5205
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5424..5528)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atcgccacca tgccgcctaa gaagaagcag aagctggaac ggctgaagaa
      601 actggacaag cccacactgc acacctgtaa caagaccagc ttcgccaagg ccttcctgcc
      661 taacgagaca taccggcagc ggctgctgga ctatatcgcc atcattcacc agctggccga
      721 ccacgcctct cacgccctga agttttacat cctgagcacc tccaccagca gcttccctgt
      781 ggtgcacgag gataccatcg aggccatcct gtacctgctg aacaagggcg aagcctggca
      841 tcccagaaaa gaggccaaga aggcctggcg ggattgcctg ctgccttacg tgcagagata
      901 ctgccagatc gtgggcttca ttcaccccaa cctgagaggc gagcagcaga gcatcaacta
      961 cctgaccgtg tccatgatga ccaacctgaa agtgaacgtg caagagcact tcatgcagat
     1021 gctgctgcgg tacatcaatc tgcgcttcga cgtgaagggc cagaagcaga gactgcctcc
     1081 taagagcgac gcccgcaagg cctttttcac ccggctgaga tacctgaagt ccgtgttcct
     1141 gttcgacgtg gtgcccgagc tggaattcct ggacgatctg acccctctgg aaagcgaggt
     1201 gctggaagag atctggtccc tggacctgcc ttttctgccc aacgatcctc tggcctacgc
     1261 catcgtggcc gatcctatga gcttcttccc agcctactgc aagctgagcg gcctgtacga
     1321 gcagtacggc ttccagcggt ttagcgccat tcctctgcgg agaagcctga tccagtctca
     1381 cgtgcggatc gacaccatca tcctgtatca gcacatcctg tgcatcacca gacgggacgc
     1441 cgagacagtg gaaaaggacg acctgtggat gagagtgtgc aacctgtgca ccaaagcctt
     1501 ccggtccaga tgcggcatgc acttcgaggg cagcatcaca acagatggcg ccagcgtgtc
     1561 cgtgtacctg aaacaccctg aggccgataa gtacggcaag agaggcgcca gaaagtccgc
     1621 caatactgtg gccgccgaag tgaaggccct gtacgtggaa aacaacctgc ctgcctgtag
     1681 agccgccgag aacgtggtgg tcatcgaccc caacaagcgg gatatcctgt actgccagga
     1741 cagcaacggc accaccttca gatacaccgc caaccagaga gccgtggaaa ccggcagcag
     1801 aagattcgcc aagcggcggg aagccatgaa ggaagaagcc ggcgttgacc tgatcgagag
     1861 cagaatccct agccacaaga ccatgaacct gatggacttc accagatacc tgctcgtgcg
     1921 gagagccgac tgggacagac ggaaagagtt ctactctcac cccgctcaca cccggtggaa
     1981 gtggcacagc ttcatcaacc ggcagaagtc cgagagcgac ctgatcagca acatgcggaa
     2041 caaatacggc gagaacttca ccgtggtcat gggcgattgg agcgacgctg gaagaaccgc
     2101 cagattccag accagcagca agaccaaagg ctggcggacc ctgttcaagc ggaaccggat
     2161 cgattgcttc ctgctggacg agtacaagac ctccagcgtg tgccctagat gcagcagcag
     2221 cgagttcgtg gaaaagaagt tcaagacccg gcctcacagc agaccctggc gaagaagaga
     2281 gggcaagatc gagaaggtgc acggcctgct gggctgcacc aatcctaatt gtctgcagca
     2341 ggcctggacc agcggcatga gatactggaa ccgggacatg ctgagcacat gcaacatgct
     2401 gctgatcgtg cggtccatgc tggatggcca tggcagacct gaggtgttct ctagatctgt
     2461 gcctgccgtg gcctgactag cataacccct tggggcgcac tcctcaggtg caggctgcct
     2521 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt
     2581 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     2641 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     2701 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     2761 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
     2821 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     2881 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     2941 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     3001 tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca
     3061 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3121 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     3181 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt
     3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3421 gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3481 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3541 caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga
     3601 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3781 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3841 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3901 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3961 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4021 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4081 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4141 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4201 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4261 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4321 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4381 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4441 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4501 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4561 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4621 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4681 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4741 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4801 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4861 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4921 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4981 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5041 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5101 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5161 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5221 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5281 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5341 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5401 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5461 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5521 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtaa
     5581 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
//
