LOCUS       TP172_pAAV_hSyn_GCaMP6m 7082 bp ds-DNA     circular     03-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      136..803
                     /label="pUC\ori"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     LTR             809..949
                     /label="L-ITR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    964..1448
                     /label="hSyn"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1461..2708
                     /label="GCaMP\6m\trunc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2715..3118
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     polyA_signal    3125..3173
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3181..4047
                     /label="mDLX"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     intron          3870..4002
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     3985..4004
                     /label="pMT2-F"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             4060..4080
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             4090..4800
                     /label="mRuby3"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4818..5068
                     /label="ssWPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     poly(A) signal  5075..5123
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     LTR             5153..5293
                     /label="R-ITR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      5385..5691
                     /label="f1\Origin"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    6210..7067
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
       61 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
      121 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      181 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      241 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      301 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      361 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      421 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
      481 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
      541 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      601 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      661 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      721 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      781 gccttttgct ggccttttgc tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact
      841 gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc
      901 gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg cggccgcacg
      961 cgtgtgtcta gactgcagag ggccctgcgt atgagtgcaa gtgggtttta ggaccaggat
     1021 gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac
     1081 ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg
     1141 aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg
     1201 cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgca
     1261 aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca
     1321 ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga
     1381 ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca
     1441 gtcgagaagg atccgccacc atggtggact catcacgtcg taagtggaat aagacaggtc
     1501 acgcagtcag agctataggt cggctgagct cactcgagaa cgtctatatc aaggccgaca
     1561 agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcggcg
     1621 tgcagctcgc ctaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1681 ccgacaacca ctacctgagc gtgcagtcca aactttcgaa agaccccaac gagaagcgcg
     1741 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1801 tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg
     1861 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1921 gcgagggtga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1981 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     2041 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     2101 gctacatcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     2161 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     2221 aggaggacgg caacatcctg gggcacaagc tggagtacaa cctgccggac caactgactg
     2281 aagagcagat cgcagaattt aaagaggctt tctccctatt tgacaaggac ggggatggga
     2341 caataacaac caaggagctg gggacggtga tgcggtctct ggggcagaac cccacagaag
     2401 cagagctgca ggacatgatc aatgaagtag atgccgacgg tgacggcaca atcgacttcc
     2461 ctgagttcct gacaatgatg gcaagaaaag ggagctacag ggacacggaa gaagaaatta
     2521 gagaagcgtt cggtgtgttt gataaggatg gcaatggcta catcagtgca gcagagcttc
     2581 gccacgtgat gacaaacctt ggagagaagt taacagatga agaggttgat gaaatgatca
     2641 gggaagcaga catcgatggg gatggtcagg taaactacga agagtttgta caaatgatga
     2701 cagcgaagaC TAGTAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGATATTC
     2761 TTAACTATGT TGCTCCTTTT ACGCTGTGTG GATATGCTGC TTTAATGCCT CTGTATCATG
     2821 CTATTGCTTC CCGTATGGCT TTCGTTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC
     2881 TTTATGAGGA GTTGTGGCCC GTTGTCCGTC AACGTGGCGT GGTGTGCTCT GTGTTTGCTG
     2941 ACGCAACCCC CACTGGCTGG GGCATTGCCA CCACCTGTCA ACTCCTTTCT GGGACTTTCG
     3001 CTTTCCCCCT CCCGATCGCC ACGGCAGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA
     3061 CAGGGGCTAG GTTGCTGGGC ACTGATAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCag
     3121 cgctAATAAA ATCGCTATCC ATCGAAGATG GATGTGTGTT GGTTTTTTGT GTGacactca
     3181 atacactcac agtggtttgg catatatttg gtgaaatttt ttaaggaaaa attagtgttg
     3241 gtttcgatat atggtagctt tttctctaac ataatttgaa taattcagca aagccctact
     3301 accagctgta cttctgcagc ctcttccatt ctttccagca ttataatttt ggttaatttt
     3361 caattttagg tcctacgtct ctgcaatttg tgtatgaata acagaataat ttccctcttt
     3421 tgtttcgcct ttcctgttcc tgaatctaaa taaagatggc tttttagtat taaaagtgga
     3481 agaaaattac aggtaattat ctttgacggt aaaaacgctg taatcagcgg gctacatgaa
     3541 aaattactct aattatggct gcatttaaga gaatggaaaa aaaccttctt gtggataaaa
     3601 accttaaatt gtccccaatg tctgcttcaa attggatggc actgcagctg gaggctttgt
     3661 tcagaattga tcctggggag ctacgaaccc aaagtttcac agtaggaagc ttgatatcgt
     3721 attcctgcag cccgggctgg gcataaaagt cagggcagag ccatctattg cttacatttg
     3781 cttctagcct gcaggtcgag gagcgcagcc ttccagaagc agagcgcggc gccttaagct
     3841 gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag
     3901 gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac
     3961 ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca
     4021 attacagctc ttaagactag aaccggtgcc accatggcac ccaagaagaa gaggaaggtg
     4081 gcggccgcta tggtgtctaa gggcgaagag ctgatcaagg aaaatatgcg tatgaaggtg
     4141 gtcatggaag gttcggtcaa cggccaccaa ttcaaatgca caggtgaagg agaaggcaga
     4201 ccgtacgagg gagtgcaaac catgaggatc aaagtcatcg agggaggacc cctgccattt
     4261 gcctttgaca ttcttgccac gtcgttcatg tatggcagcc gtacctttat caagtacccg
     4321 gccgacatcc ctgatttctt taaacagtcc tttcctgagg gttttacttg ggaaagagtt
     4381 acgagatacg aagatggtgg agtcgtcacc gtcacgcagg acaccagcct tgaggatggc
     4441 gagctcgtct acaacgtcaa ggtcagaggg gtaaactttc cctccaatgg tcccgtgatg
     4501 cagaagaaga ccaagggttg ggagcctaat acagagatga tgtatccagc agatggtggt
     4561 ctgagaggat acactgacat cgcactgaaa gttgatggtg gtggccatct gcactgcaac
     4621 ttcgtgacaa cttacaggtc aaaaaagacc gtcgggaaca tcaagatgcc cggtgtccat
     4681 gccgttgatc accgcctgga aaggatcgag gagagtgaca atgaaaccta cgtagtgcaa
     4741 agagaagtgg cagttgccaa atacagcaac cttggtggtg gcatggacga gctgtacaag
     4801 taattaGGCG CGCCaggGAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG
     4861 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT
     4921 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTAG
     4981 TTCTTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC
     5041 TGTTGGGCAC TGACAATTCC GTGGTGTTag cgctAATAAA AGATCTTTAT TTTCATTAGA
     5101 TCTGTGTGTT GGTTTTTTGT GTGGAATTCc cacgtgcgga ccgagcggcc gcaggaaccc
     5161 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     5221 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     5281 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     5341 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     5401 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5461 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5521 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5581 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5641 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5701 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5761 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     5821 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5881 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5941 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6001 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6061 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6421 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6721 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7081 aa
//