LOCUS       Exported                6998 bp ds-DNA     circular SYN 04-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 5, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      43..710
                     /label=pUC ori
     repeat_region   716..856
                     /label=AAV2 ITR
     intron          1188..2125
                     /label=EF-1-alpha intron A
     protein_bind    2165..2198
                     /label=lox2272
     protein_bind    2249..2282
                     /label=loxP
     CDS             complement(2291..3001)
                     /label=mNeonGreen
     gene            complement(2291..3001)
                     /label=mNeonGreen
     misc_feature    complement(3011..3572)
                     /label=WT_IRES_273_836
     misc_feature    complement(3011..3013)
                     /label=AUG834
     misc_feature    complement(3019..3021)
                     /label=AUG 827
     misc_feature    complement(3061..3167)
                     /label=JK_Loop
     misc_feature    complement(3072..3077)
                     /label=A6
     misc_feature    complement(3581..4345)
                     /label=mScarlet
     misc_feature    complement(4277..4345)
                     /label=Myr/Palm Extended Ser/Thre phosphatase Mouse
     protein_bind    complement(4358..4391)
                     /label=lox2272
     protein_bind    complement(4442..4475)
                     /label=loxP
     misc_feature    4488..4891
                     /label=WPRE
     polyA_signal    4898..4946
                     /label=poly(A) signal
     repeat_region   4976..5116
                     /label=AAV2 ITR
     rep_origin      5191..5646
                     /label=f1 ori
     promoter        5928..6032
                     /label=AmpR promoter
     CDS             6033..6893
                     /label=bla
ORIGIN
        1 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
       61 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
      121 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
      181 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      241 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      301 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      361 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      421 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      481 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      541 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      601 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      661 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtcctgc
      721 aggcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
      781 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      841 atcactaggg gttcctgcgg ccgcacgcgt aagctttgca aagatggata aagttttaaa
      901 cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct
      961 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     1021 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     1081 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     1141 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     1201 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     1261 ccactggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     1321 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     1381 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     1441 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     1501 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     1561 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     1621 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     1681 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     1741 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     1801 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     1861 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     1921 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     1981 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     2041 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     2101 tcaaagtttt tttcttccat ttcaggtgtc gtgaggtacc ggatcctcta gagtcgactc
     2161 cggaataact tcgtatagga tactttatac gaagttatgc agaatggtag ctggattgta
     2221 gctgctatta gcaatatgaa acctcttaat aacttcgtat agcatacatt atacgaagtt
     2281 atggcgcgcc ttacttgtac agctcgtcca tgcccatcac atcggtaaag gccttttgcc
     2341 actccttgaa gttgagctcg gtcttggagt gcttgagctc cgtcttacgg aacacgtaca
     2401 tcggctggtt cttcagatag ttagccgcca ttggcttggc aaaggtgtag gtggtccgcg
     2461 cagtgctccg gtagcgcttg ccatttccag tggtgtaact ccacttaaag gtactgatga
     2521 tggttttgtc gttggggtaa gtcttcttcg acctgcacca gtccgcagcg gtcagcgagt
     2581 tggtcatcac aggaccgtca gcagggaaac cagtcccctt cacctgggcc tctcctttga
     2641 tgtggcttcc ctcgtaggtg tagcggtagt taacagtaag ggaggcacca tcttcaaact
     2701 gcattgtgcg atggacttgg tatccagagc catctaccat agcggcctgg aaaggcgaca
     2761 tcccgtcagg gtagggcagg tactgatgga agccataccc gatatgaggg accagaatcc
     2821 agggggagaa ctggaggtcg cccttggtgg acttcaggtt taactcctca taaccatcat
     2881 ttggattgcc ggtgccctga cccaccatgt caaagtccac accgttgatg gagccaaaga
     2941 tgtgtaactc atgtgtcgct gggagagagg ccatgttatc ctcctcgccc ttgctcacca
     3001 tagcgatcgc catattatca tcgtgttttt caaaggaaaa ccacgtcccc gtggttcggg
     3061 gggcctagac gttttttaac ctcgactaaa cacatgtaaa gcatgtgcac cgaggcccca
     3121 gatcagatcc catacaatgg ggtaccttct gggcatcctt cagccccttg ttgaatacgc
     3181 ttgaggagag ccatttgact ctttccacaa ctatccaact cacaacgtgg cactggggtt
     3241 gtgccgcctt tgcaggtgta tcttatacac gtggcttttg gccgcagagg cacctgtcgc
     3301 caggtggggg gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt cttcaagaag
     3361 cttccagagg aactgcttcc ttcacgacat tcaacagacc ttgcattcct ttggcgagag
     3421 gggaaagacc cctaggaatg ctcgtcaaga agacagggcc aggtttccgg gccctcacat
     3481 tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac cggccttatt
     3541 ccaagcggct tcggccagta acgttagggg ggggcgtacg ctacttgtac agctcgtcca
     3601 tgccgccggt ggagtggcgg ccctcggagc gttcgtactg ttccaccacg gtgtagtcct
     3661 cgttgtggga ggtgatgtcc aacttgcggt cgacgttgta ggcgccgggc atctgcacgg
     3721 gcttcttggc cttgtaggtg gtcttgaagt ccgccaggta gcgtccgccg tccttcaggc
     3781 gcagggccat cttaatgtcg cccttcagca cgccgtcctc ggggtacaac cgctcggtgg
     3841 acgcttccca gcccattgtc ttcttctgca ttacggggcc gtcaggaggg aagttggtgc
     3901 cgcggagctt caccttgtag atcagggtgc cgtcctccag ggaggtgtcc tgggtcacgg
     3961 tcacggcgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
     4021 actgcttata gtagtcgggg atgtcggcgg ggtgcttggt gaaggccctg gagccgtaca
     4081 tgaactgagg ggacaggatg tcccaggaga agggcagggg gccacccttg gtcaccttca
     4141 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
     4201 cgtggccgtt catggagccc tccatgtgca ccttgaaccg catgaactcc ttgatcactg
     4261 cctcgccctt gctcactggt tgagtcttcg aatctacttc atcgtcattg agattgtctt
     4321 tcctttttga tttaatacat cccatggtgg cgctagcata acttcgtata aagtatccta
     4381 tacgaagtta tttgccttaa cccagaaatt atcactgtta ttctttagaa tggtgcaaag
     4441 aataacttcg tataatgtat gctatacgaa gttatgaatt caagcttaat caacctctgg
     4501 attacaaaat ttgtgaaaga ttgactgata ttcttaacta tgttgctcct tttacgctgt
     4561 gtggatatgc tgctttaatg cctctgtatc atgctattgc ttcccgtatg gctttcgttt
     4621 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtcc
     4681 gtcaacgtgg cgtggtgtgc tctgtgtttg ctgacgcaac ccccactggc tggggcattg
     4741 ccaccacctg tcaactcctt tctgggactt tcgctttccc cctcccgatc gccacggcag
     4801 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc taggttgctg ggcactgata
     4861 attccgtggt gttgtcgggg aagctgacgt cagcgctaat aaaatcgcta tccatcgaag
     4921 atggatgtgt gttggttttt tgtgtgggta acccacgtgc ggaccgagcg gccgcaggaa
     4981 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     5041 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     5101 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     5161 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     5221 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5281 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5341 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5401 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5461 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5521 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5581 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     5641 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     5701 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5761 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5821 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     5881 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5941 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6001 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6061 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6121 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6181 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6241 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6301 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6361 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6421 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6481 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6541 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6601 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6661 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6721 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6781 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6841 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6901 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6961 tctaggtgaa gatccttttt gataatctca tgaccaaa
//
