LOCUS       TP133_pAAV_Cbh_DIO_hTyr 6080 bp ds-DNA     circular     30-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     repeat_region   1..130
                     /label="AAV2 ITR (alternate)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     enhancer        145..430
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        432..709
                     /label="chicken beta-actin promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     intron          710..937
                     /label="hybrid intron"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    967..1000
                     /label="lox2272"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    1051..1084
                     /label="loxP"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             complement(1093..2730)
                     /label="Translation 2730-1093"
                     /translation="MLLAVLYCLLWSFQTSAGHFPRACVSSKNLMEKECCPPWSGDRSPCGQLSGRGSCQNILLSNAPLGPQFPFTGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFWGPNCTERRLLVRRNIFDLSAPEKDKFFAYLTLAKHTISSDYVIPIGTYGQMKNGSTPMFNDINIYDLFVWMHYYVSMDALLGGSEIWRDIDFAHEAPAFLPWHRLFLLRWEQEIQKLTGDENFTIPYWDWRDAEKCDICTDEYMGGQHPTNPNLLSPASFFSSWQIVCSRLEEYNSHQSLCNGTPEGPLRRNPGNHDKSRTPRLPSSADVEFCLSLTQYESGSMDKAANFSFRNTLEGFASPLTGIADASQSSMHNALHIYMNGTMSQVQGSANDPIFLLHHAFVDSIFEQWLRRHRPLQEVYPEANAPIGHNRESYMVPFIPLYRNGDFFISSKDLGYDYSYLQDSDPDSFQDYIKSYLEQASRIWSWLLGAAMVGAVLTALLAGLVSLLCRHKRKQLPEEKQPLLMEKEDYHSLYQSHLAAGSGEFYPYDVPDYA*"
     misc_feature    complement(1096..1122)
                     /label="HA tag"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(1123..1143)
                     /label="Linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(1144..2737)
                     /label="hTyr"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    complement(2748..2781)
                     /label="lox2272"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     protein_bind    complement(2832..2865)
                     /label="loxP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(2935..2955)
                     /label="WPRE-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     repeat_region   3343..3472
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   3343..3483
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      3558..4013
                     /label="f1 ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(3645..3664)
                     /label="F1ori-R"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     3855..3876
                     /label="F1ori-F"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(4030..4049)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     4149..4171
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(4209..4227)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        4295..4399
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             4400..5260
                     /label="AmpR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(4618..4637)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      5431..6019
                     /label="ori"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     5920..5939
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgttac ataacttacg gtaaatggcc cgcctggctg
      181 accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga ctttccattg
      241 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      301 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc
      361 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      421 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
      481 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
      541 gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag
      601 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
      661 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
      721 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      781 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
      841 ttagctgagc aagaggtaag ggtttaaggg atggttggtt ggtggggtat taatgtttaa
      901 ttacctggag cacctgcctg aaatcacttt ttttcaggtt ggggatcctc tagagtcgac
      961 tccggaataa cttcgtatag gatactttat acgaagttat gcagaatggt agctggattg
     1021 tagctgctat tagcaatatg aaacctctta ataacttcgt atagcataca ttatacgaag
     1081 ttatggcgcg ccTTAAGCGT AATCTGGAAC ATCGTATGGG TAAAACTCCC CGGACCCAGC
     1141 TGcTAAATGG CTCTGATACA AGCTGTGGTA ATCCTCTTTC TCCATGAGGA GTGGCTGCTT
     1201 TTCTTCAGGA AGCTGCTTTC TCTTGTGACG ACACAGCAAG CTCACAAGCC CTGCCAGCAG
     1261 GGCAGTGAGG ACGGCCCCTA CCATCGCCGC CCCAAGGAGC CATGACCAGA TCCGACTCGC
     1321 TTGTTCCAAA TAGGACTTAA TGTAGTCTTG AAAAGAGTCT GGGTCTGAAT CTTGTAGATA
     1381 GCTATAGTCA TAGCCCAGAT CTTTGGATGA AATAAAGAAA TCACCATTTC TGTACAGTGG
     1441 TATAAAAGGA ACCATGTAGG ATTCCCGGTT ATGTCCAATG GGTGCATTGG CTTCTGGATA
     1501 AACTTCTTGA AGAGGACGGT GCCTTCGGAG CCACTGCTCA AAAATACTGT CAACAAATGC
     1561 ATGGTGAAGA AGGAAGATAG GATCGTTGGC AGATCCCTGT ACCTGGGACA TTGTTCCATT
     1621 CATATAGATG TGCAAGGCAT TGTGCATGCT GCTTTGAGAG GCATCCGCTA TCCCAGTAAG
     1681 TGGACTAGCA AATCCTTCCA GTGTATTTCT AAAGCTGAAA TTGGCAGCTT TATCCATGGA
     1741 ACCAGATTCA TATTGGGTCA AACTCAGGCA AAATTCTACA TCAGCTGAAG AGGGGAGCCT
     1801 TGGGGTTCTG GATTTGTCAT GGTTTCCAGG ATTACGCCGT AAAGGTCCCT CGGGCGTTCC
     1861 ATTGCATAAA GACTGATGGC TGTTGTACTC CTCCAATCGG CTACAGACAA TCTGCCAAGA
     1921 GGAGAAGAAT GATGCTGGGC TGAGTAAGTT AGGATTTGTG GGGTGCTGAC CTCCCATGTA
     1981 CTCATCTGTG CAAATGTCAC ACTTTTCTGC ATCCCGCCAG TCCCAATATG GAATAGTGAA
     2041 GTTTTCATCT CCTGTCAGCT TCTGGATTTC TTGTTCCCAC CGCAACAAGA AGAGTCTATG
     2101 CCAAGGCAGA AAAGCTGGTG CTTCATGGGC AAAATCAATG TCTCTCCAGA TTTCAGATCC
     2161 CCCAAGCAGT GCATCCATTG ACACATAATA ATGCATCCAG ACAAAGAGGT CATAAATATT
     2221 GATGTCGTTA AACATGGGTG TTGATCCATT TTTCATTTGG CCATAGGTCC CTATGGGGAT
     2281 GACATAGTCT GAGCTGATGG TATGCTTTGC TAAAGTGAGG TAGGCAAAAA ATTTGTCCTT
     2341 CTCTGGGGCA CTCAAATCGA AGATGTTTCT TCTCACCAAG AGTCGTCTCT CTGTGCAGTT
     2401 TGGTCCCCAA AAGCCAAACT TGCAGTTTCC ACAGTTGAAT CCCATGAAGT TGCCAGAGCA
     2461 CTGGCAGGTC CTATTATAAA AGACGGAAGG CCACGACTCC CGGTCATCCA CCCCTGTGAA
     2521 GGGAAATTGA GGCCCAAGTG GTGCATTGGA CAGAAGGATA TTCTGACAGG AACCTCTGCC
     2581 TGAAAGCTGG CCACAGGGAC TCCTGTCCCC GCTCCACGGT GGACAGCATT CCTTCTCCAT
     2641 CAGGTTCTTA GAGGAGACAC AGGCTCTAGG GAAATGGCCA GCGGAGGTCT GGAAACTCCA
     2701 CAGCAGGCAG TACAAAACAG CCAGGAGCAT CAAGGTCtTA ggctagcata acttcgtata
     2761 aagtatccta tacgaagtta tttgccttaa cccagaaatt atcactgtta ttctttagaa
     2821 tggtgcaaag aataacttcg tataatgtat gctatacgaa gttatgaatt ccgctcgaga
     2881 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2941 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3001 tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact
     3061 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3121 cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca tctagcttta
     3181 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     3241 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
     3301 tttaaagcgg ccgcggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     3361 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     3421 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     3481 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     3541 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3601 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     3661 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3721 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     3781 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3841 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
     3901 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
     3961 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4021 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4081 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4141 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4201 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4261 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4321 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4381 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4441 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4501 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4561 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4621 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4681 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4741 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4801 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4861 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4921 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4981 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5041 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5101 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5161 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5221 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5281 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5341 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5401 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5461 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5521 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5581 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5641 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5701 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5761 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5821 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5881 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5941 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6001 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6061 ctggcctttt gctcacatgt
//