LOCUS       TP224_pAAV_CKII(0.4)_Az 5834 bp ds-DNA     circular     29-SEP-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        156..518
                     /label="a-CaMKII(0.4)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    545..1585
                     /label="PPO_AZ"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1586..1612
                     /label="bRho-M4"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1613..1630
                     /label="Linker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1637..1696
                     /label="ts"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             1700..2395
                     /label="mScarlet"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             2396..2596
                     /label="Kv2.1 targeting domain"
                     /ApEinfo_revcolor="#000000"
                     /ApEinfo_fwdcolor="#000000"
     misc_feature    2609..3012
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     polyA_signal    3019..3067
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     repeat_region   3097..3237
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      3312..3767
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        4049..4153
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             4154..4222
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             4223..5014
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      5185..5773
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcttgt ggactaagtt tgttcgcatc
      181 cccttctcca accccctcag tacatcaccc tgggggaaca gggtccactt gctcctgggc
      241 ccacacagtc ctgcagtatt gtgtatataa ggccagggca aagaggagca ggttttaaag
      301 tgaaaggcag gcaggtgttg gggaggcagt taccggggca acgggaacag ggcgtttcgg
      361 aggtggttgc catggggacc tggatgctga cgaaggctcg cgaggctgtg agcagccaca
      421 gtgccctgct cagaagcccc aagctcgtca gtcaagccgg ttctccgttt gcactcagga
      481 gcacgggcag gcgagtggcc cctagttctg ggggcagctc tagagcggta ccgGATCCgc
      541 caccATGGAG AGTGTTGCCT TCCACCGGAA CTCTTCATCA TCTAATAGCA GCGCCTACAC
      601 GGAGCTCTTG TCTCGGACTG GTTACACAAT CCTGGCCATT ATCATGGGCG CGTTCTCTGT
      661 AGGAGGGATC ATACTCAATG TCTTGGTAAT TGTGGTGACA GTGAGACACA GACAGCTTAG
      721 GCAGCCCCTC AGCTATGCCC TCGTTAACCT GGCAGTTTGC GACCTGGGCT GCGCTGTGTT
      781 CGGGGGCCTT CCCACGACAG TAACCAGTGC CATGGGATAC TTCAGCCTGG GAAGGGTGGG
      841 CTGTGTGTTA GAAGCCTTCG CTGTTTCCTT CTTCGGTATA GCAAGTCTGT GTACAGTAGC
      901 AGTCATCTCT GTGGAACGCT ACATTGTGGT GTGCTATCCC ATGGGTGCAG TCCTGTTCCA
      961 GACCAGGCAT GCCGTTGGCG GGGTGGTCCT GTCCTGGGTG TGGTCGTTTG TGTGGAGCAC
     1021 TCCACCTCTG TTTGGGTGGG GAACGTTTGA GTTGGAGGGC GTCCAAACCA GTTGTGCTCC
     1081 TAACTGGTAT AGCCGCGATA TTGGGGACAT GTCCTACATG ATCATGTTCT TTGTCCTTTG
     1141 CTTTGCCGTG CCCTTTTCCA TCATCATGGT GTCCTACTCA CGACTTCTCT GGACCCTCCG
     1201 CCAGGTGACC AAGCTGCAGG TGTCCGAAAC AGGTAGCACA AATCGTGTGG AGGTGCAGGT
     1261 GGCACGGATG ATAGTGGTGA TGGTGTTGGC CTTCCTAGTC ACCTGGCTGC CATATGCCTC
     1321 AATGGCTGTC GCTGTCATCA TGGATTCCAG TCTATATGTA GACCCTGTCA TCGCGACCAT
     1381 CCCTGTATAC TTTGCAAAAA GTAGCACTGT CTACAACCCG ATCATTTATA TTTTCATGAA
     1441 CAGACAGTTT CGAGGCTACA CTGTTGCTGC TGTTCTGTGT GGATGGAACC CATGGTCCTC
     1501 AGAGCCACAA ACTTCTGAGA ATGAGACCAC TGTTACATCT GTCAGCAAGA CCCCCCAAAA
     1561 GATTGTGCCT GAAAAATCGT TAGAAaccga gaccagccag gtggctcctg ctCCGCGaGC
     1621 CCGaGATCCA ACCGGTAAGA GCAGGATCAC CAGCGAGGGC GAGTACATCC CCCTGGACCA
     1681 GATCGACATC AACGTGGTGa tggtgagcaa gggcgaggca gtgatcaagg agttcatgcg
     1741 gttcaaggtg cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
     1801 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     1861 cctgcccttc tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcac
     1921 caagcacccc gccgacatcc ccgactacta taagcagtcc ttccccgagg gcttcaagtg
     1981 ggagcgcgtg atgaacttcg aggacggcgg cgccgtgacc gtgacccagg acacctccct
     2041 ggaggacggc accctgatct acaaggtgaa gctccgcggc accaacttcc ctcctgacgg
     2101 ccccgtaatg cagaagaaga caatgggctg ggaagcgtcc accgagcggt tgtaccccga
     2161 ggacggcgtg ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gcggacgcta
     2221 cctggcggac ttcaagacca cctacaaggc caagaagccc gtgcagatgc ccggcgccta
     2281 caacgtcgac cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca
     2341 gtacgaacgc tccgagggcc gccactccac cggcggcatg gacgagctgt acaagcagtc
     2401 ccagcctatt ctgaacacca aagagatggc cccccagagt aaaccccctg aggagctgga
     2461 aatgtcctca atgccctccc ctgtggcacc tcttcctgca agaacagagg gcgtgatcga
     2521 catgaggtcc atgagttcta ttgactcttt tatctcatgc gccaccgatt ttccagaggc
     2581 tacgcggttt aagtaagAAT TCaagcttAA TCAACCTCTG GATTACAAAA TTTGTGAAAG
     2641 ATTGACTGAT ATTCTTAACT ATGTTGCTCC TTTTACGCTG TGTGGATATG CTGCTTTAAT
     2701 GCCTCTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCGTT TTCTCCTCCT TGTATAAATC
     2761 CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC CGTCAACGTG GCGTGGTGTG
     2821 CTCTGTGTTT GCTGACGCAA CCCCCACTGG CTGGGGCATT GCCACCACCT GTCAACTCCT
     2881 TTCTGGGACT TTCGCTTTCC CCCTCCCGAT CGCCACGGCA GAACTCATCG CCGCCTGCCT
     2941 TGCCCGCTGC TGGACAGGGG CTAGGTTGCT GGGCACTGAT AATTCCGTGG TGTTGTCGGG
     3001 GAAGCTGACG TCagcgctAA TAAAATCGCT ATCCATCGAA GATGGATGTG TGTTGGTTTT
     3061 TTGTGTGggt aacccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     3121 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3181 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     3241 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     3301 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3361 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3421 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3481 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     3541 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3601 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     3661 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     3721 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     3781 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     3841 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     3901 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     3961 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4021 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4081 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4141 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4201 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4261 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4321 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4381 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4441 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4501 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4561 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4621 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4681 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4741 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4801 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4861 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4921 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4981 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5041 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5101 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5161 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5221 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5281 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5341 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5401 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5461 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5521 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5581 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5641 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5701 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5761 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5821 ttttgctcac atgt
//