LOCUS       pInt_attP1_ampR         2115 bp ds-DNA     circular     03-JUL-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..48
                     /label="bxb1_attP"
     misc_feature    24..25
                     /label="central_di_nt"
     misc_feature    49..68
                     /label="Spacer"
     misc_feature    complement(69..116)
                     /label="FRT"
     primer          complement(212..233)
                     /label="pInt_seq_rev"
                     /note="sequence: cccagtagctgacattcatccg"
     gene            477..1337
                     /label="bla"
     misc_feature    1360..1406
                     /label="voigt_ter_L3S3P11"
     rep_origin      1616..2038
                     /label="R6K gamma replication origin"
     primer          1684..1705
                     /label="pInt_seq_fwd"
                     /note="sequence: gcgttacatccctggcttgttg"
     misc_feature    complement(2050..2095)
                     /label="FRT"
     misc_feature    2096..2115
                     /label="Spacer"
ORIGIN
        1 ggtttgtctg gtcaaccacc gcggtctcag tggtgtacgg tacaaaccgt gtaggctgga
       61 gctgcttcga agttcctata ctttctagag aataggaact tcggaatagg aacttcaaga
      121 tcccccacgc tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta
      181 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
      241 gacaagggaa aacgcaagcg caaagagaaa gcaggtaACG AAAGGGCCTC GTGATACGCC
      301 TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC
      361 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC
      421 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA
      481 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT
      541 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG
      601 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG
      661 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG
      721 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG
      781 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA
      841 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG
      901 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC
      961 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG
     1021 CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC
     1081 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG
     1141 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG
     1201 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA
     1261 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC
     1321 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATC CAATTATTGA ACACCCTTCG
     1381 GGGTGTTTTT TTGTTTCTGG TCTCCCttcg aaatgaccga ccaagcgacg cccaacctgc
     1441 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     1501 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     1561 accccagctt caaaagcgct ctaaggagga tattcatatg gaccatggct aattcccatg
     1621 tcagccgtta agtgttcctg tgtcactcaa aattgctttg agaggctcta agggcttctc
     1681 agtgcgttac atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc
     1741 ttatatattc tttttttctt ataaaactta aaaccttaga ggctatttaa gttgctgatt
     1801 tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag agcttagtac
     1861 gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa catgagagct
     1921 tagtacgtta aacatgagag cttagtacgt gaaacatgag agcttagtac gtactatcaa
     1981 caggttgaac tgctgatctt cagatcctct acgccggacg catcgtggcc ggatcttgcg
     2041 gccgcaaaag aagttcctat actttctaga gaataggaac ttcggaatag gaactattac
     2101 acgtcttgag cgatt
//