LOCUS       pInt_attP2_kanR         1994 bp ds-DNA     circular     03-JUL-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..48
                     /label="bxb1_attP"
     misc_feature    24..24
                     /label="SNP"
     misc_feature    24..25
                     /label="central_di_nt"
     misc_feature    49..68
                     /label="Spacer"
     misc_feature    complement(69..116)
                     /label="FRT"
     primer          complement(212..233)
                     /label="pInt_seq_rev"
                     /note="sequence: cccagtagctgacattcatccg"
     CDS             476..1270
                     /label="kanamycin resistance"
     rep_origin      1494..1917
                     /label="R6K gamma replication origin"
     primer          1562..1583
                     /label="pInt_seq_fwd"
                     /note="sequence: gcgttacatccctggcttgttg"
     misc_feature    complement(1929..1974)
                     /label="FRT"
     misc_feature    1975..1994
                     /label="Spacer"
ORIGIN
        1 ggtttgtctg gtcaaccacc gcgTtctcag tggtgtacgg tacaaaccgt gtaggctgga
       61 gctgcttcga agttcctata ctttctagag aataggaact tcggaatagg aacttcaaga
      121 tcccccacgc tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta
      181 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
      241 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
      301 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
      361 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
      421 ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat
      481 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
      541 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
      601 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
      661 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
      721 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
      781 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
      841 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
      901 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
      961 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     1021 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     1081 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     1141 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     1201 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     1261 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     1321 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     1381 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1441 cccagcttca aaagcgctct aaggaggata ttcatatgga ccatggctaa ttcccatgtc
     1501 agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag ggcttctcag
     1561 tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt
     1621 atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt
     1681 atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
     1741 ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt
     1801 agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg tactatcaac
     1861 aggttgaact gctgatcttc agatcctcta cgccggacgc atcgtggccg gatcttgcgg
     1921 ccgcaaaaga agttcctata ctttctagag aataggaact tcggaatagg aactattaca
     1981 cgtcttgagc gatt
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