LOCUS       Exported                5615 bp ds-DNA     linear   UNA 04-JUL-2023
DEFINITION  pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA Sequencing 
            Result.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     labeled by gh
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          1..812
                     /organism="pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::Bs
                     aI-sgRNA Sequencing Result"
                     /mol_type="other DNA"
                     /label=source:pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-
                     bGHp
                     /label=source:pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-
                     bGHpA;U6: :BsaI-sgRNA Sequencing Result
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA
                     -
                     bGHpA;U6: :BsaI-sgRNA Sequencing Result"
     source          2870..5615
                     /organism="pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::Bs
                     aI-sgRNA Sequencing Result"
                     /mol_type="other DNA"
                     /label=source:pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA- b
                     GH
                     /label=source:pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA- b
                     GHpA;U6: :BsaI-sgRNA Sequencing Result
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA
                     - b
                     GHpA;U6: :BsaI-sgRNA Sequencing Result"
     misc_feature    1..130
                     /locus_tag="L-ITR"
                     /label=L-ITR
     promoter        155..731
                     /locus_tag="CMV_immearly_promoter"
                     /label=CMV_immearly_promoter
     misc_feature    234..521
                     /locus_tag="CAG_enhancer"
                     /label=CAG_enhancer
     misc_feature    688..708
                     /locus_tag="CMV_fwd_primer"
                     /label=CMV_fwd_primer
     misc_feature    768..788
                     /locus_tag="SV40 NLS "
                     /label=SV40 NLS
     misc_feature    813..2030
                     /locus_tag="ISAba30_humanized"
                     /label=ISAba30_humanized
     misc_feature    2031..2078
                     /locus_tag="nucleoplasmin NLS"
                     /label=nucleoplasmin NLS
     misc_feature    2085..2165
                     /locus_tag="3XHA"
                     /label=3XHA
     misc_feature    complement(2193..2210)
                     /locus_tag="BGH_rev_primer"
                     /label=BGH_rev_primer
                     /note="/translation=MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRS
                     LVCRF 
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR 
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     terminator      2196..2399
                     /locus_tag="bGH_PA_terminator"
                     /label=bGH_PA_terminator
                     /note="/translation=MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRS
                     LVCRF 
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR 
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     misc_feature    2419..2424
                     /label=BsaI
                     /label=BsaI(1)
     misc_feature    2431..2436
                     /label=BsaI
     misc_feature    complement(2438..2620)
                     /locus_tag="gRNA_bb_ISAba30"
                     /label=reRNA_bb_ISAba30
     misc_feature    complement(2621..2869)
                     /locus_tag="U6 promoter"
                     /label=U6 promoter
     misc_feature    complement(2679..2698)
                     /locus_tag="LKO1_5_primer"
                     /label=LKO1_5_primer
                     /note="/translation=MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRS
                     LVCRF 
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR 
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     misc_feature    2878..3018
                     /locus_tag="R-ITR"
                     /label=R-ITR
     rep_origin      3110..3416
                     /locus_tag="f1_origin"
                     /label=f1_origin
                     /note="/translation=MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRS
                     LVCRF 
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR 
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     misc_feature    3684..3706
                     /locus_tag="pGEX_3_primer"
                     /label=pGEX_3_primer
                     /note="/translation=MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRS
                     LVCRF 
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR 
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     promoter        3865..3893
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/translation=MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRS
                     LVCRF 
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR 
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     gene            3935..4795
                     /gene="Ampicillin"
                     /locus_tag="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRS
                     LVCRF 
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR 
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     CDS             3935..4795
                     /codon_start=1
                     /locus_tag="ORF frame 3"
                     /label=ORF frame 3
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4950..5569
                     /locus_tag="pBR322_origin"
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gactcgaggc gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctctc tggctaacta ccggtgccac catggcccca aagaagaagc
      781 ggaaggtcgg tatccacgga gtcccagcag ccatgaagcg gctgcaggct ttcaagtacg
      841 agatggtgcc caacggacag caggagaggc agatgagaag attcgccggg agctgccggt
      901 tcgtgtacaa caaggccctg gccctgcaga aggagagata cgagagagga gaaaagaagc
      961 tggggtacgc cggcctgtgc aagctgctga cagagtggag gaacagcagc gagatggctt
     1021 ggctggccga cgctcctgtg catcctctgc agcagaccct gaaggatctg gagagagcct
     1081 acagcaattt cttcgccaag cgcgccgact ttcccagatt caaaaagaag ggctgtcact
     1141 ccagcttccg ctaccccgat cccaaacaga tcaaactgga gcagcacaac agcagaatct
     1201 tcctgccaaa gctgggctgg attagatacc gcaagagtag agacgtgctg ggagccgtga
     1261 aaaacgtgac cgtgagtctg agcggcggga aatggttcgt gagcatccag acagagcggg
     1321 aagtggagca gcccatccct cagggaggag ctgtgggaat tgacatgggg atcgccagat
     1381 tcgctaccat tagcgacgga aagttctacg ctcccctgaa cagcttcaaa agacacgaga
     1441 agagactgag atgcgcccag caggccctgt ctagaaaggt gaagttcagc aacaactggc
     1501 agaaagccaa agccagaatc cagagaatcc atagcagaat cggcaacgcc agaagagact
     1561 tcctgcacaa gaccagcacc gccatcagcc agaaccatgc catggtgtgc atcgaggacc
     1621 tgcaggtgag aaacatgagc aagagcgccg ccggaacccc tgaacagcct ggaagaaacg
     1681 tgagagccaa aagcggcctg aacaagagca tcctggacca gggatggttc gaatttagaa
     1741 gacagctgga ctacaagctg gcctggaacg gaggctggct ggtggctgtg cctcctaaaa
     1801 acaccagcag aacctgcccc tgctgcgctc atgtgagcgc tgataacaga cagacccagg
     1861 agagatttga gtgcatcgag tgtggcttcg agggcaacgc tgacgtggtg ggagctatca
     1921 acgtgctgag agccggccac gctagactgg cttgtgaggt gagcgacgcc gtgggaagtc
     1981 cagctgctgg aacacaccgg agcgactctg atcaggccca ctgtagggtg aaaaggccgg
     2041 cggccacgaa aaaggccggc caggcaaaaa agaaaaaggg atcctaccca tacgatgttc
     2101 cagattacgc ttacccatac gatgttccag attacgctta cccatacgat gttccagatt
     2161 acgcttaaga attcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     2221 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     2281 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     2341 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata gcaggcatgc
     2401 tggggaggta ccaaaaaaga gacctgccgt ggtctccttg acatcctccc cggcctgaag
     2461 gccggagatt cctacggcgc tcagacgcgg caatgagcct ggtctgagtc gcttcggtgg
     2521 gttcctgctg ctggcgaccc tactgcatcg ctcacttcac aggctaaccg ggcgtgcccc
     2581 gcccttagta cattgatcgc gccgaccaca tcggcattac ggtgtttcgt cctttccaca
     2641 agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata tgatagtcca
     2701 ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt ctacgtcacg
     2761 tattttgtac taatatcttt gtgtttacag tcaaattaat tccaattatc tctctaacag
     2821 ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctcg cggccgcagg
     2881 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     2941 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3001 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3061 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     3121 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     3181 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3241 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3301 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3361 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3421 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     3481 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     3541 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     3601 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     3661 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     3721 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     3781 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3841 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3901 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3961 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4021 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4081 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4141 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4201 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4261 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4321 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4381 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4441 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4501 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4561 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4621 tgctgataaa tctggagccg gtgagcgtgg aagccgcggt atcattgcag cactggggcc
     4681 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4741 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4801 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4861 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4921 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4981 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5041 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5101 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5161 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5221 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5281 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5341 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5401 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5461 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5521 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5581 gttcctggcc ttttgctggc cttttgctca catgt
//
