LOCUS       pCBad33_pISAam1_        5447 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    N3 mpCBad33-pISN3(Aam1)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             75..1181
                     /codon_start="1"
                     /note="ISAam1"
                     /note="color: #00ff00"
                     /translation="MVNKSYKFRLYPTKEQEQLLAKTFGCVRFVYNKMLEERIQIYEK
                     FKDDKEALKKQTFPTPAKYKKEFPWLKEVDSLALANAQLNLQKAFQNFFSGRAGFPKF
                     KNRKAKQSYTTNVVNGNIQLSDGYIKLPKLKWVKFKQHREIPAHHIIKACTIKKTKTG
                     KYYVSILTEYEHQPVPKEIQTVVGLDFSMNGLFVDSEGKRANYPRFYRQALEKLAKEQ
                     RILSRRKKGSNRWHKQRLKVAKLHEKIANQRKDFLHKKSYELAKQYDCVVIENLNMKG
                     MSQVLNFGKSVHDNGWGMFTTFLQYKLEEQGKKLIKIDKWFPSSKTCSCCDQVKESLS
                     LSERTFRCECGFESDRDVNAAINIKHEGMKRLAIA"
                     /locus_tag="ISAam1"
                     /label=ISAam1
                     /ApEinfo_fwdcolor=#80ff80
                     /ApEinfo_revcolor=#e9d024
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1192..1220
                     /note="modified J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffc0cb"
                     /locus_tag="modified J23119 promoter"
                     /label=modified J23119 promoter
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1421..1440
                     /note="gRGS"
                     /note="color: #99ccff"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1441..1446
                     /note="PolyT terminator"
                     /note="color: #d6b295"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3435..4094)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4456..5372
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1221..1420
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaga attcatggta aacaaatcat ataaattccg tctgtaccca acaaaagaac
      121 aagaacagct gcttgccaaa accttcggtt gtgtccgttt cgtttataac aaaatgcttg
      181 aagaacgcat acaaatttat gaaaagttca aagacgacaa agaagctttg aaaaaacaaa
      241 catttccgac ccctgccaag tacaaaaagg agtttccttg gcttaaagaa gtggatagcc
      301 ttgcgttagc aaacgcccaa ttgaatttgc agaaagcgtt tcaaaatttc ttttcgggtc
      361 gtgctggatt tccaaagttc aaaaaccgca aggcgaaaca gtcgtacacc acaaatgtgg
      421 tcaatggcaa tattcaactt tcagatggct atatcaagtt acccaaactg aaatgggtca
      481 agttcaagca acatcgggag attcctgccc accatatcat caaggcttgt acgatcaaga
      541 aaacaaaaac aggaaaatac tatgtttcta ttctcaccga atacgaacat caacctgtcc
      601 ccaaagaaat acaaacggtt gttgggcttg atttttccat gaatggttta tttgtcgata
      661 gcgagggtaa gagagccaat tatcctcgtt tctatcggca agcattggaa aaattagcga
      721 aagaacaacg gatattatca cgccgaaaga agggttctaa tcgttggcac aaacagcgtt
      781 tgaaagtagc gaagctacat gagaaaatag cgaaccaacg aaaagacttt ctacacaaaa
      841 aatcatacga attagcgaaa cagtatgatt gcgtggtcat cgaaaacctc aacatgaaag
      901 ggatgtcgca agtcctgaat ttcggcaaga gcgttcatga caacggctgg gggatgttca
      961 cgacatttct ccaatacaag ttagaggagc aagggaaaaa gcttatcaaa atagataagt
     1021 ggtttccgtc atctaaaact tgttcatgtt gcgatcaagt aaaggaatct ttatcgcttt
     1081 ctgagcgtac attccgctgt gaatgtggat tcgagagcga cagggacgtc aatgcggcaa
     1141 tcaatatcaa acatgagggc atgaaacgat tagcaatagc ctgagagatc tttgacagct
     1201 agctcagtcc taggtaaata gaatgtggat tcgagagcga cagggacgtc aatgcggcaa
     1261 tcaatatcaa acatgagggc atgaaacgat tagcaatagc ctaacttgtc ctcgaaccgt
     1321 gggacacacg gggatcgctc agtcaacttc ccgtcatgag atgggattac ctgagaagcc
     1381 cccacctcta agcgaagcgt aggtggtggg agcatgtcac gctcggagat catcattgcg
     1441 ttttttaagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
     1501 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
     1561 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
     1621 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     1681 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     1741 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggg cgggcaggac
     1801 gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt
     1861 ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1921 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1981 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2041 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttggggca aactattaac
     2101 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2161 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2221 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2281 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2341 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2401 ctcatatata ctttagattg atttacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     2461 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2521 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2581 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2641 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     2701 tggagtccac gttctttaat agtggactct tgttccaaac ttgaacaaca ctcaacccta
     2761 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     2821 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     2881 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2941 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3001 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3061 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3121 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3181 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtcaggca tttgagaagc
     3241 acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg
     3301 ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc
     3361 attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa
     3421 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     3481 agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc
     3541 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     3601 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     3661 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     3721 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     3781 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     3841 catatcacca gctcaccgtc tttcattgcc atacggaact ccggatgagc attcatcagg
     3901 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     3961 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     4021 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     4081 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     4141 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     4201 tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt
     4261 atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt
     4321 gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc
     4381 tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag
     4441 caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag
     4501 ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga
     4561 atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     4621 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     4681 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
     4741 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     4801 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
     4861 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
     4921 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     4981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
     5041 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
     5101 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
     5161 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
     5221 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt
     5281 attaatcaga taaaatattt ctaggctcat gagcccgaag tggcgagccc gatcttcccc
     5341 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
     5401 cacgatgcgt ccggcgtaga ggatctgctc atgtttgaca gcttatc 
//
