LOCUS       pCBad33_pISAba30        5580 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    59 mpCBad33-pIS59(Aba30)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #a6acb3"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #a6acb3"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    81..1298
                     /note="ISAba30"
                     /note="color: #a6acb3"
                     /locus_tag="ISAba30"
                     /label=ISAba30
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1315..1349
                     /note="J23119 promoter modified to end with an SpeI si"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffffff"
                     /locus_tag="J23119 promoter modified to end with an SpeI
                     site"
                     /label=J23119 promoter modified to end with an SpeI site
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1554..1573
                     /note="gRGS"
                     /note="color: #a6acb3"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1574..1579
                     /note="PolyT terminator"
                     /note="color: #a6acb3"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3568..4227)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #a6acb3; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4589..5505
                     /note="p15a ori"
                     /note="color: #a6acb3"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1350..1553
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaga attcgaattc atgaaacgac ttcaagcctt caagtacgaa atggtaccaa
      121 acggccagca ggaacggcag atgcgccgct tcgctggctc gtgccggttc gtctacaaca
      181 aggcactggc gttgcagaag gagcgctacg agcgcggtga gaagaagctc ggctatgccg
      241 ggctgtgcaa gctactcacc gagtggcgca atagctcgga aatggcatgg ttggctgatg
      301 cgccggttca cccgctgcaa cagacgctca aggacttgga gcgggcgtat tccaacttct
      361 ttgccaagcg cgccgacttc ccgcgcttca aaaagaaggg gtgccacagt agctttcgct
      421 atcccgatcc gaaacaaatc aagctggagc agcacaacag ccgcatcttt ctgcccaagc
      481 ttggctggat tcgctaccgc aagagccgcg atgtgctggg ggcggtgaag aacgtcactg
      541 tcagcctctc gggcggcaag tggttcgtgt ccattcaaac cgaacgagag gtcgagcagc
      601 ccatcccgca gggtggcgcg gtcggtatcg acatgggcat tgcccggttc gctacgatct
      661 cggacggcaa gttctacgct cctctaaaca gcttcaagcg acacgaaaag cgcttgcgct
      721 gcgcgcagca agcgctctcc cgcaaggtga aattcagcaa caactggcag aaggcgaaag
      781 cccgtatcca gcgcatccat tcccgcatcg gcaacgcccg ccgcgacttc ctgcacaaga
      841 cctcgaccgc gatcagccaa aaccacgcga tggtgtgtat cgaggacttg caggtacgga
      901 acatgtccaa gtcggcggcg ggtactcccg aacagccggg aagaaacgtt cgggccaagt
      961 ccggcctgaa caaatccatc ctcgatcagg gctggttcga gttccgtcgc caactggatt
     1021 ataaactggc gtggaatggt gggtggctcg ttgccgtgcc gcctaagaac accagccgaa
     1081 cctgcccgtg ttgtgctcat gtgtcggcgg ataatcgcca gacacaagag cggttcgagt
     1141 gcatagagtg cgggttcgag ggtaatgccg atgtggtcgg cgcgatcaat gtactaaggg
     1201 cggggcacgc ccggttagcc tgtgaagtga gcgatgcagt agggtcgcca gcagcaggaa
     1261 cccaccgaag cgactcagac caggctcatt gccgcgtcgg atcctgagag atctttgaca
     1321 gctagctcag tcctaggtat aatactagtg catagagtgc gggttcgagg gtaatgccga
     1381 tgtggtcggc gcgatcaatg tactaagggc ggggcacgcc cggttagcct gtgaagtgag
     1441 cgatgcagta gggtcgccag cagcaggaac ccaccgaagc gactcagacc aggctcattg
     1501 ccgcgtctga gcgccgtagg aatctccggc cttcaggccg gggaggatgt caagctcgga
     1561 gatcatcatt gcgtttttta agcttggctg ttttggcgga tgagagaaga ttttcagcct
     1621 gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag
     1681 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
     1741 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa
     1801 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     1861 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
     1921 gggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
     1981 acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa
     2041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2101 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2161 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2221 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2281 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2341 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2401 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2461 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2521 cagaccaagt ttactcatat atactttaga ttgatttacg cgccctgtag cggcgcatta
     2581 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2641 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2701 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2761 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2821 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aacttgaaca
     2881 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
     2941 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3001 acgtttacaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3061 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3121 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3181 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3241 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3301 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtcag
     3361 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
     3421 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt
     3481 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcac caggcgttta
     3541 agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat cgcagtactg
     3601 ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat gatgaacctg
     3661 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     3721 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     3781 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     3841 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     3901 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     3961 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga actccggatg
     4021 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     4081 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     4141 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     4201 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     4261 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     4321 gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg
     4381 gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtattt attcggcgca
     4441 aagtgcgtcg ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg
     4501 ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac ggggtggtgc
     4561 gtaacggcaa aagcaccgcc ggacatcagc gctagcggag tgtatactgg cttactatgt
     4621 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
     4681 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
     4741 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
     4801 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
     4861 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
     4921 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
     4981 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
     5041 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
     5101 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5161 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
     5221 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
     5281 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
     5341 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
     5401 agaagatcat cttattaatc agataaaata tttctaggct catgagcccg aagtggcgag
     5461 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     5521 cggtgatgcc ggccacgatg cgtccggcgt agaggatctg ctcatgtttg acagcttatc
//
