LOCUS       pCBad33_pISAeme8        5519 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    7 mpCBad33-pIS7(Aeme8)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    75..1217
                     /note="ISAeme8"
                     /note="color: #00ff00"
                     /locus_tag="ISAeme8"
                     /label=ISAeme8
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1234..1268
                     /note="J23119 promoter modified to end with an SpeI si"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ff99cc"
                     /locus_tag="J23119 promoter modified to end with an SpeI
                     site"
                     /label=J23119 promoter modified to end with an SpeI site
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1493..1512
                     /note="gRGS"
                     /note="color: #a6acb3"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1513..1518
                     /note="PolyT terminator"
                     /note="color: #ffcc99"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3507..4166)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4528..5444
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1269..1492
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaga attcatgact ttgagaagag caacgaaagt acgcatttat ccgaccgacg
      121 aacaagcggc attcttgaat gcccagttcg gcgcggtgcg gttcgcgtac aacaaggccc
      181 ttcatattca acgacacatg ttcaagcgtc acgggatttc actgaaaccg aaacgcgacc
      241 tgaaacccat gctcgcagtg gcgaaaaaat cgcgcaaata cggctggcta aaggagtacg
      301 attcactcgc cttgcagcaa gcggtgatta atctcgacaa ggcattcgcc aacttcttca
      361 atcccaagct caaggcacga atgcctacct tcaagagcaa acggggcagg caatccagct
      421 atcaccccaa cggaaaggtg ctcacagcgg ctatccagtt gccgaagatg acgcccatca
      481 gggcggtcat tcatcgagat attatcggcg tggtatccag catcacagtc agccgtggcc
      541 cgacagggaa atactatgct tccatccttt gcgaggatgg ctgcgaggct cccgccaagc
      601 ccaccttcat cacggacgtg actggctgtg atatggggct gtcccactac ctcattgagt
      661 cgagcggcaa aaagattgcc aatccacgcc accttatcaa cgccagtcgc aatctacgac
      721 gcaagcaaaa ggcactgtct cgcaagaaaa agggcagcgc caaccgtggc aaggcaagac
      781 ggcaactggc cgccctgcac gaacgggtag ccaatgcccg cgccgatttt cagcacaaac
      841 tctctcacac gatggttgac gaaaaccaag cggtcatcgt ggagacattg aaatcagcga
      901 acatgatgaa aaatcacaaa ctggcccgcg ccattggcga tgctggttgg catggtttca
      961 tcatgaagct ggagtacaag gcacaagcgg cgggtcgcca cctaatcaaa cttgatcagt
     1021 ggtttgccag ctcgaaactg tgtagcgatt gcggccacaa gatgccggac atgccgttgc
     1081 atcagcggca gtgggtatgt ccagcgtgtg gggcagggca tgaccgcgac attaacgcgg
     1141 ccatgaatat ccagcaacaa ggcatattgg aattaaaggc ggcgggacac gtcgtttcta
     1201 cccatggagg ccagcgtgga tcctgagaga tctttgacag ctagctcagt cctaggtata
     1261 atactagtgc cggacatgcc gttgcatcag cggcagtggg tatgtccagc gtgtggggca
     1321 gggcatgacc gcgacattaa cgcggccatg aatatccagc aacaaggcat attggaatta
     1381 aaggcggcgg gacacgtcgt ttctacccat ggaggccagc gttaatctgc caattcggca
     1441 gtagcggcct aagaagtggg aagcctcgcc gcttgcggcg gggagcagtc acgctcggag
     1501 atcatcattg cgttttttaa gcttggctgt tttggcggat gagagaagat tttcagcctg
     1561 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
     1621 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
     1681 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
     1741 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     1801 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     1861 ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga
     1921 cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat
     1981 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2041 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     2101 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggg
     2161 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2221 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2281 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2341 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2401 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2461 agaccaagtt tactcatata tactttagat tgatttacgc gccctgtagc ggcgcattaa
     2521 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2581 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2641 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2701 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2761 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa acttgaacaa
     2821 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     2881 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2941 cgtttacaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3001 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3061 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3121 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3181 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3241 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtcagg
     3301 catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat
     3361 agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt
     3421 cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa
     3481 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt
     3541 tgtaattcat taagcattct gccgacatgg aagccatcac agacggcatg atgaacctga
     3601 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     3661 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     3721 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     3781 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     3841 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     3901 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ctccggatga
     3961 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     4021 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     4081 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     4141 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     4201 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     4261 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     4321 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa
     4381 agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     4441 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
     4501 taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt
     4561 ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg
     4621 tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac
     4681 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     4741 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     4801 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     4861 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     4921 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     4981 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     5041 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5101 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     5161 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     5221 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     5281 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     5341 gaagatcatc ttattaatca gataaaatat ttctaggctc atgagcccga agtggcgagc
     5401 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     5461 ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatc
//
