LOCUS       pCBad33_pISAs26_        5594 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    2 mpCBad33-pIS2(As26)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    75..1298
                     /note="ISAs26"
                     /note="color: #00ff00"
                     /locus_tag="ISAs26"
                     /label=ISAs26
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1315..1349
                     /note="J23119 promoter modified to end with an SpeI si"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ff99cc"
                     /locus_tag="J23119 promoter modified to end with an SpeI
                     site"
                     /label=J23119 promoter modified to end with an SpeI site
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1568..1587
                     /note="gRGS"
                     /note="color: #a6acb3"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1588..1593
                     /note="PolyT terminator"
                     /note="color: #a6acb3"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3582..4241)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #a6acb3; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4603..5519
                     /note="p15a ori"
                     /note="color: #a6acb3"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1350..1567
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaga attcatgcaa attctgaaag cctacaaatt ccgactcgaa ccgaccgaac
      121 aacaagcgca acgcctgcgc cagttgggcg gttgtgctcg ttatgtctgg aatcacgggc
      181 tggcagaaac caaacgcatt ctggatgctg gcgaaaaact gccttctgcc tttgagctga
      241 acaggatgct caccagttgg aagaaagacc cagaaatggc ctttctggcc gaggcatata
      301 ccgacaacct ccagcagaag ctcaaggatt tgcatggcgc gtggatgcgc tgctttgata
      361 agaaactcgc cgccaaggca cccgtattca agaaaaaaag cgacaaccgc gacgctatcc
      421 ggttcgtcaa cttcgacaag tattgccgcc ttgaaaaggg tcgcgtgaag ctgcctgccg
      481 ggctggcgtg ggtcaagttc cgtcagtccc agccagttga agggaaaatt aaaaacgcga
      541 ccgtcagcca agcagccggg cactggtata tctcctttca ggtcgagcag gagacgcttg
      601 agccaattca cccctccggc tcaatggtcg ggctagatgc ggggatcgcc aagctcgcaa
      661 ccctgtcaga cggcacggtg tttgagcccg tcaacagctt caaaaccaat cagaccaagc
      721 tggcaaggct ccaacgccag ttgagcaaaa aggtaaagtt cagcagcaac tggaagaaac
      781 aaaaagccaa gatccagcgg ctgcactcac atatcgccaa tacccgccgc gattaccttc
      841 acaaggtcac aacgactatc agcaaaaacc acgcgatgat cgtcattgag gatttgaagg
      901 ttgccagcat gtcaaaatca gcagcgggca cgaccagcca gccggggcgc aacgtccggg
      961 caaaatcagg gctgaatcgt tcgatattag atcagggctg gtttgagctg cgccgccagc
     1021 tcgaatataa gcaggcatgg cgaggcggtc aggtgttggc gataaacccg gcctacacca
     1081 gccagcagtg cgcctgttgt gggcatactg cgaaggataa ccgccagacg caaagccagt
     1141 ttaaatgcgc tgcgtgtggt tatgaggcga acgccgatgt gaacggcgct cgcaatattt
     1201 tagcggcggg acacgccgtt ttagcctgtg gaggcgtggt gcaatcaggc cgcccgatga
     1261 agcaggaacc cggtactgcg cgagcagcta tcacccaggg atcctgagag atctttgaca
     1321 gctagctcag tcctaggtat aatactagtg ccagtttaaa tgcgctgcgt gtggttatga
     1381 ggcgaacgcc gatgtgaacg gcgctcgcaa tattttagcg gcgggacacg ccgttttagc
     1441 ctgtggaggc gtggtgcaat caggccgccc gatgaagcag gaacccggta ctgcgcgagc
     1501 agctatcacc cagtaatggg aatccccctc ctgtgacgcg aagcgctaag gtgggggagg
     1561 atgtcaagct cggagatcat cattgcgttt tttaagcttg gctgttttgg cggatgagag
     1621 aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat
     1681 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa
     1741 cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca
     1801 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1861 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca
     1921 acggcccgga gggtgggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc
     1981 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc
     2041 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2101 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2161 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2221 gaagatcagt tggggcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2281 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2341 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2401 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2461 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2521 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt tacgcgccct
     2581 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2641 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     2701 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     2761 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     2821 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     2881 tccaaacttg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     2941 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3001 ttaacaaaat attaacgttt acaatttaaa aggatctagg tgaagatcct ttttgataat
     3061 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3121 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3181 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3241 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     3301 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3361 ctgttaccag tcaggcattt gagaagcaca cggtcacact gcttccggta gtcaataaac
     3421 cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgaccg
     3481 ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta ttcaggcgta
     3541 gcaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc gccctgccac
     3601 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacagacg
     3661 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     3721 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     3781 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     3841 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     3901 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     3961 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     4021 cggaactccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     4081 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     4141 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     4201 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     4261 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     4321 ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc ccagggcttc
     4381 ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc cgtcacaggt
     4441 atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tactgattta gtgtatgatg
     4501 gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac
     4561 tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc ggagtgtata
     4621 ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa
     4681 aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc
     4741 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg
     4801 gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc
     4861 aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa
     4921 tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc
     4981 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
     5041 gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
     5101 actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc
     5161 ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat
     5221 ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt
     5281 ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc
     5341 ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga
     5401 ccaaaacgat ctcaagaaga tcatcttatt aatcagataa aatatttcta ggctcatgag
     5461 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     5521 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctgctcatg
     5581 tttgacagct tatc    
//
