LOCUS       pCBad33_pISCbt1_        5896 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    19 mpCBad33-pIS19(Cbt1)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    75..1553
                     /note="ISCbt1"
                     /note="color: #00ff00"
                     /locus_tag="ISCbt1"
                     /label=ISCbt1
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1570..1599
                     /note="J23119 promoter "
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ff99cc"
                     /locus_tag="J23119 promoter "
                     /label=J23119 promoter 
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1870..1889
                     /note="gRGS"
                     /note="color: #a6acb3"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1890..1895
                     /note="PolyT terminator"
                     /note="color: #ffcc99"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3884..4543)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #a6acb3; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4905..5821
                     /note="p15a ori"
                     /note="color: #a6acb3"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1600..1869
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaga attcatggct aaatcaaaaa catcaagtta tatattaact ttaaaattaa
      121 aaacagaaaa gtatcaagaa gatatattaa ataaaagact agaaatcagc agaaatatat
      181 ataactcttg tttaggtgaa atattaaaaa gatataaaca tacaagagaa ttgaaagact
      241 ataggaaaga atataaaaat ataattaaaa tgagtaaaga caaagaacgt agtaaaaaat
      301 ttaatgaatt aaataaaaaa tatgatttaa ctgagtattc acttcataca tatgttaaac
      361 caattcaaaa gcattttaaa gataatgtag atagttttac agctcaaaag atagcaacaa
      421 gagtttttaa ttcatttcag aaactaatgt ttcatcaagc taaaagagta tattttaaaa
      481 gatatggtga attaaatagt gttgagggta aatctaataa aactggcatt agatttcaag
      541 ataattcgtt aatatggaat gggttaaaaa tacctgtaat aataaaaact aatgatatat
      601 atgttcaaat gtcattagaa aatagagtta agtactgtag aatcatcaga aggattgtaa
      661 aaggtaaaat caaattttat gttcaattaa ttcttgaagg aatacctcct atgaagatta
      721 ataagactac aggcgagata aaaaataaga taggtaaagg taatgtgggg atagatatag
      781 gtacaagaac tattgcagta tctagtgaaa atgatgttaa gttattagag ttagcacctg
      841 agattgataa tatagaaaat caaaaaagaa tattaaatag gaaattagat agacaaagac
      901 gtgctaataa tcctaataag tataatgaag atggaactat taataggaat aataaagata
      961 aatggatcaa atctaataga tatattaaaa ctcaaaataa acttaaagaa attcaaagga
     1021 aacaagctga aatcagaaaa caaagtcatt atagactaat aaatagattg ttactattgg
     1081 gtaatagatt tttagtagaa accatgaatt ataagggact acaggctaga gtaaaagaaa
     1141 ctactataaa tgagaaaaca ggtagattca ataaaaagaa aagatttggt aagagtctag
     1201 cgaataaagc acctagtatg tttttaacta tgttagataa taagctaaaa tggaataata
     1261 ctcaactatt caaaatagat actaagaaat gcaaagcaag tcaatataat cattttacaa
     1321 atgaatattg taagaaagag ttaaaagaca gatggaatga agatattcaa attcaaagag
     1381 atatgtattc tgcattttta ataatgaatg ttgtaggtaa aacattagat aaaatagata
     1441 gagatttatg tattgaaact tatgataatt ttaggagatt acacgacaaa gagatagaaa
     1501 gattaaaaga attaaagaaa aatggatata aattaatatc cagtatgggg ataggatcct
     1561 gagagatctt tgacagctag ctcagtccta ggtataatag agattacacg acaaagagat
     1621 agaaagatta aaagaattaa agaaaaatgg atataaatta atatccagta tggggatata
     1681 aaatctatat aggtttagac atgagcctta tactaatgtt aatcatttat taaaatgata
     1741 attagtgaaa gtcttagaga aactaactag tatttatatg ttgtaattta tttgaagttt
     1801 aaataaatga gagtataaaa gaagttaatg tatctaagaa ccccaaggct ttagtcttgg
     1861 gagtgtcagg ctcggagatc atcattgcgt tttttaagct tggctgtttt ggcggatgag
     1921 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     1981 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
     2041 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
     2101 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2161 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
     2221 caacggcccg gagggtgggc gggcaggacg cccgccataa actgccaggc atcaaattaa
     2281 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
     2341 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2401 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2461 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     2521 ctgaagatca gttggggcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2581 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2641 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2701 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2761 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2821 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc
     2881 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2941 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3001 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3061 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     3121 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3181 gttccaaact tgaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     3241 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3301 ttttaacaaa atattaacgt ttacaattta aaaggatcta ggtgaagatc ctttttgata
     3361 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3421 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3481 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3541 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     3601 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3661 tcctgttacc agtcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
     3721 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac
     3781 cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg
     3841 tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc
     3901 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga
     3961 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     4021 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     4081 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     4141 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     4201 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     4261 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     4321 tacggaactc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     4381 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     4441 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     4501 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     4561 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     4621 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct
     4681 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag
     4741 gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga
     4801 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct
     4861 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta
     4921 tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag
     4981 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc
     5041 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
     5101 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
     5161 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     5221 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
     5281 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     5341 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     5401 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
     5461 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
     5521 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
     5581 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
     5641 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
     5701 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc taggctcatg
     5761 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     5821 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctgctca
     5881 tgtttgacag cttatc    
//
