LOCUS       pCBad33_ISClsp11        5455 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    N5 mpCBad33-pISN5(Clsp11)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    69..1184
                     /note="ISClsp11"
                     /note="color: #00ff00"
                     /locus_tag="ISClsp11"
                     /label=ISClsp11
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1195..1224
                     /note="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffc0cb"
                     /locus_tag="J23119 promoter"
                     /label=J23119 promoter
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1429..1448
                     /note="gRGS"
                     /note="color: #99ccff"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1449..1454
                     /note="PolyT terminator"
                     /note="color: #d6b295"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3443..4102)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4464..5380
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1225..1428
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaat gaacgtagta taccggtttc ggatctatcc caataaaata cagcaggaac
      121 tgtttgcaaa aacatttgga tgtgtcaggt tcgtatataa ccggatgctg tcagagaaga
      181 aggaatgcta tgaaaagacc ggaaaaaatc taaaggtcac tccggcaaaa tataaggcag
      241 aatttccatg gctaaaagaa gtggacagcc ttgctctgtg caatgcccag ctgcacctac
      301 agattgcata taaaaacttt ttccgggatc catcgtttgg ttttccgaga ttcaagtcaa
      361 aaaagaatcc tgttaaaagc tataccacaa acagtgtcaa tggaaatatc ctgttaaaag
      421 atgggaagct gaagctcccc aaaactggct ggatccggat ccggcagcat cgtaagatcc
      481 gggaagatgc ctgcctgaaa ggcgcaaccg tctgtatgga agctgatggg cggtattatg
      541 tttctcttct gtatttttgt gaagaaaaga cggtggaaac aaggaacata aggcaggccg
      601 tcggactcga tttttccatg aaagaactat atgtagattc caatggaaaa catgcaggtt
      661 atccgcacta tttcagaaac agccaggaaa aactggcgaa agcgcagaga aaactgagtc
      721 attgccggaa agaaagcaat cgttacaaaa aacagcagaa aaaagtggcg cggatccata
      781 cacatatcgc tcaccagaga aaagattact tgcataaaga atcaagaaag ataaccaatt
      841 tctatgatat cgtatgtatc gaagatctgg atctgaaaac gatgagcggg gaacatcatt
      901 ttggaaaaag tgtccatgat aacggctgga gaatgttcac agattttttg cagtataaac
      961 tggaacggga agggaagaaa cttgtccgca tcgaccgctg gtatcccagt tccagaacat
     1021 gcagctgttg tggaaaaata aaacatgatc tgaaactgga agagcgggta tatctttgta
     1081 actgcggaaa ccggatggac cgtgatgaaa atgcggcggt caatatctgc cgggaaggcc
     1141 tgagaatatc cgggatcata ttagaaaaaa gcgaaaaagc atcatgagag atctttgaca
     1201 gctagctcag tcctaggtat aatagaaaat gcggcggtca atatctgccg ggaaggcctg
     1261 agaatatccg ggatcatatt agaaaaaagc gaaaaagcat cataaaaaga tatcagtcca
     1321 aaagaaccgt gggacacacg gggatcgctc gtgaatgctt tgcccaacgg ggcaatcgaa
     1381 cgagaagccc ccacttcaag cgtgagctaa gtggtgggag tatgtcacgc tcggagatca
     1441 tcattgcgtt ttttaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac
     1501 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
     1561 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
     1621 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     1681 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     1741 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtgggcg
     1801 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga
     1861 tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt
     1921 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1981 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2041 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttggggcaaa
     2101 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2161 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2221 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2281 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2341 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2401 caagtttact catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc
     2461 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2521 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2581 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2641 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     2701 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact
     2761 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     2821 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     2881 tacaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2941 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3001 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3061 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3121 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtcaggcatt
     3241 tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac
     3301 ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa
     3361 tttctgccat tcatccgctt attatcactt attcaggcgt agcaccaggc gtttaagggc
     3421 accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta
     3481 attcattaag cattctgccg acatggaagc catcacagac ggcatgatga acctgaatcg
     3541 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     3601 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     3661 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     3721 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     3781 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     3841 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaactcc ggatgagcat
     3901 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     3961 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     4021 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     4081 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     4141 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     4201 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     4261 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     4321 cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca
     4381 gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac
     4441 ggcaaaagca ccgccggaca tcagcgctag cggagtgtat actggcttac tatgttggca
     4501 ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg
     4561 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
     4621 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
     4681 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
     4741 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
     4801 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
     4861 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     4921 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     4981 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
     5041 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
     5101 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
     5161 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
     5221 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
     5281 atcatcttat taatcagata aaatatttct aggctcatga gcccgaagtg gcgagcccga
     5341 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     5401 atgccggcca cgatgcgtcc ggcgtagagg atctgctcat gtttgacagc ttatc
//
