LOCUS       pCBad33_pISClte2        5503 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    27 mpCBad33-dIS27(Clte2)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    75..1211
                     /note="ISClte2"
                     /note="color: #00ff00"
                     /locus_tag="ISClte2"
                     /label=ISClte2
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1228..1257
                     /note="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffc0cb"
                     /locus_tag="J23119 promoter"
                     /label=J23119 promoter
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1477..1496
                     /note="gRGS"
                     /note="color: #a6acb3"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1497..1502
                     /note="PolyT terminator"
                     /note="color: #d6b295"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3491..4150)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4512..5428
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1258..1476
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaga attcatgtgt caactgttgg tggtgctcca ctttcagcaa ttaaacaata
      121 tattgaaaat caaaaaacat cacaaaggaa atgatactgt gcaattaacc attaaaatca
      181 aattaatacc aatgaaagag caaaaactac atattcaaga tacttgttta gaatatatta
      241 aaactgttaa tgatatagta tcactaatgg ttgctgaaaa taaaagtctt aaactaactt
      301 caaaagatat aattgctaat cttccaagtg ctgttaaaaa tcaagcaatt agagatagta
      361 aaagtgtata ttctaaatat aagaaaacta aaatacaaaa ttttcttaaa aaacctatgt
      421 gtatatggaa caaccaaaac tatagtattg gtgaaaatta tattgctttt ccagtaatga
      481 ttaatggtaa atctaaaaag attaaagtta aagctatcat agaacaatat caaaaagatt
      541 tattaaccaa taaattaggt actttaagaa taactaaaaa agccaataaa ttaatggctc
      601 aaatttctat tgatgtagta gaaaaagaaa atgtaaacaa taaagctatg ggtatagatt
      661 tgggactaaa aatacctgct gttgcagtaa cagaagataa aaaagtaaaa ttctttggca
      721 atggtaggca aaacaaatac attaaaagaa aatatagatc attaagaaaa aagttaggta
      781 aagctaaaaa aattaacaaa ataaaatcta taaatgataa agaacaaaga tatatgaaag
      841 accaagacca caaaataagt agaggaatag ttaattttgc tattcaaaat aatatttccg
      901 taattagatt agaaaaacta actaatatca gaaatacggc aaaaacaaac cgtaaaaatg
      961 aaaagaattt gcatacttgg tcattctatc gtctagcaca atacattgaa tataaggcac
     1021 ttttagaagg tattaaaatt gaatatgtta atccatgtaa tacatctaaa caatgcccta
     1081 tttgtggcaa attaaatact gctaaagata gaaaatatca atgttcatgt ggttataaag
     1141 cacatagaga tttgaatgct tgtattaata taattaatgc aactgttgta gatggtaata
     1201 gtctacaagc cggatcctga gagatctttg acagctagct cagtcctagg tataatacta
     1261 gtgtggttat aaagcacata gagatttgaa tgcttgtatt aatataatta atgcaactgt
     1321 tgtagatggt aatagtctac aagcctaaga tactataggt actgtcttag gaagggtact
     1381 ggcataccct tagcttgagg tcatactgga ctatcagaaa tgaactgtcc tttaatcact
     1441 caagaatccc ctagatttat ctatggggag tgtcaagctc ggagatcatc attgcgtttt
     1501 ttaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
     1561 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
     1621 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
     1681 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
     1741 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     1801 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtgggcggg caggacgccc
     1861 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
     1921 gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1981 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2041 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2101 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt ggggcaaact attaactggc
     2161 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     2221 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     2281 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     2341 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     2401 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     2461 tatatacttt agattgattt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2521 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2581 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2641 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     2701 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     2761 gtccacgttc tttaatagtg gactcttgtt ccaaacttga acaacactca accctatctc
     2821 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     2881 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaaa
     2941 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3001 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3061 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3121 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3181 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3241 caccgcctac atacctcgct ctgctaatcc tgttaccagt caggcatttg agaagcacac
     3301 ggtcacactg cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat
     3361 ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc
     3421 atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc
     3481 cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     3541 ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca
     3601 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     3661 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     3721 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     3781 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     3841 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     3901 tcaccagctc accgtctttc attgccatac ggaactccgg atgagcattc atcaggcggg
     3961 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     4021 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     4081 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     4141 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     4201 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
     4261 attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt
     4321 attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg
     4381 ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt
     4441 tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc
     4501 gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg
     4561 tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat
     4621 gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
     4681 gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat
     4741 acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct
     4801 gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa
     4861 agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg
     4921 tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag
     4981 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
     5041 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc
     5101 accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg
     5161 gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc
     5221 ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt
     5281 cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta
     5341 atcagataaa atatttctag gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     5401 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     5461 atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atc 
//
