LOCUS       pCBad33_ISCvi1_R        5427 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    N12 mpCBad33-pISN12(Cvi1)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    69..1157
                     /note="ISCvi1"
                     /note="color: #00ff00"
                     /locus_tag="ISCvi1"
                     /label=ISCvi1
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1168..1197
                     /note="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffc0cb"
                     /locus_tag="J23119 promoter"
                     /label=J23119 promoter
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1401..1420
                     /note="gRGS"
                     /note="color: #99ccff"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1421..1426
                     /note="PolyT terminator"
                     /note="color: #d6b295"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3415..4074)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4436..5352
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1198..1400
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaat gaacaaaggt gttaagttta gggcgtaccc caataaggaa caacaaaact
      121 taataaaccg aacactcgga tgttgcaggc tcatctacaa caaaggtctt gctatgcgta
      181 acgatgccta ttccaatggc gaaaagattg gctataacca aacatctgct atgctcacaa
      241 accttaaaaa gcaggctgac tttgctttcc tgaaggatgt tgattctatt gcactccagc
      301 agtctttgag ggatttagac cgtggattta aaaatttctt tgagaagcgt gcaagacatc
      361 cgcagttcaa aagtaagcat aataaccacc agtcatacag aaccatcaat cagggcgata
      421 acatccgcat aatcgggaag tatattaagc tcccgaagct cgggtatgtc aaaatcaaac
      481 agtccatgga agtcgggcat atcaataatg tcacggtaga gcgtactcct acgggtaaat
      541 attttgtagt tctgaatgtt gactttgagc cagagtttcg accaaatgca ggctgcatga
      601 ttggcattga tgtcggcata aaggagtttt actccgacag caacggcaat gtcgtcaata
      661 accctaagta tttagagaag tcaatgcgta aactcgccag agaacaacgc aggctttctc
      721 gtaagcaaaa aggctctaat aatcgcaata gacagcgtgt taaggtagct aaagtccatg
      781 aaaagataac taatcagcgt aacgatttcc ttcaaaaaca gtcaacgata cttgttagcg
      841 aaaaccaaac gatctgtatc gaagacctga atgtaaaagg aatgattcgt aatcataaac
      901 ttgcacaaca catagcaagc tgttcatggt ctaagttctt tgcaatgctt gaatacaagg
      961 ctatatggta cggaaatgac ataatcaaag tacctactat gtatccaagc agtcagacat
     1021 gcagttgttg tgggtataag aacccgtttg taaagaatct ctcaatcaga gaatgggaat
     1081 gtcctaattg tcacgcaaag cacgatagag acaccaacgc aagcataacc atcttaaata
     1141 aagggctgtc aatagcctga gagatctttg acagctagct cagtcctagg tataatagca
     1201 aagcacgata gagacaccaa cgcaagcata accatcttaa ataaagggct gtcaatagcc
     1261 tgacatatat aaaataccgt agggcatacg ggaatttacg cttgtggaca ctgtgtaaga
     1321 cgagccaaca aggtaacttg ttaggaacgc agtagtggtt gaagcaagaa tcccacgact
     1381 ttagtcgtgt ggagtgtcaa gctcggagat catcattgcg ttttttaagc ttggctgttt
     1441 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
     1501 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
     1561 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
     1621 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1681 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     1741 acgttgcgaa gcaacggccc ggagggtggg cgggcaggac gcccgccata aactgccagg
     1801 catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     1861 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1921 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1981 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2041 agtaaaagat gctgaagatc agttggggca aactattaac tggcgaacta cttactctag
     2101 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     2161 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     2221 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2281 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2341 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2401 atttacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2461 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2521 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2581 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     2641 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2701 agtggactct tgttccaaac ttgaacaaca ctcaacccta tctcgggcta ttcttttgat
     2761 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2821 tttaacgcga attttaacaa aatattaacg tttacaattt aaaaggatct aggtgaagat
     2881 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2941 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3001 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3061 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3121 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3181 cgctctgcta atcctgttac cagtcaggca tttgagaagc acacggtcac actgcttccg
     3241 gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct
     3301 gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac
     3361 ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc
     3421 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     3481 gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     3541 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     3601 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     3661 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     3721 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     3781 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     3841 tttcattgcc atacggaact ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     3901 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     3961 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     4021 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     4081 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     4141 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt
     4201 ggcccagggc ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc
     4261 ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat
     4321 ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct
     4381 cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct
     4441 agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca
     4501 tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat
     4561 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg
     4621 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg
     4681 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
     4741 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4801 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     4861 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
     4921 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc
     4981 ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc
     5041 actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga
     5101 aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt
     5161 agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga
     5221 gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt
     5281 ctaggctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
     5341 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
     5401 ggatctgctc atgtttgaca gcttatc   
//
