LOCUS       pCBad33_ISCvi2_R        5469 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    N13 mpCBad33-pISN13(Cvi2)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    69..1202
                     /note="ISCvi2"
                     /note="color: #00ff00"
                     /locus_tag="ISCvi2"
                     /label=ISCvi2
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1213..1242
                     /note="J23119 promoter "
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffc0cb"
                     /locus_tag="J23119 promoter"
                     /label=J23119 promoter
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1443..1462
                     /note="sgRGS"
                     /note="color: #99ccff"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1463..1468
                     /note="PolyT terminator"
                     /note="color: #d6b295"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3457..4116)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4478..5394
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1243..1442
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaat gaataaagca atcaaataca gagtgcatcc cacaactgaa caatctgtta
      121 tgtttgctaa gacctttggc tgttgtcgta aggtctataa tcttatgctt tccgataaaa
      181 ttgacggcta caaagctacg ggcaaatttc ctactgttac gcctgccaaa tataagaacg
      241 attatcctta tcttagggaa gtggatagtc ttgctcttgc caataagcaa atggacttac
      301 aggaagcgtt ccgcaataca tttagcaaat cccgcaaaaa gaaaaatggt tttcctaaat
      361 tcaaatccgc aaagcatagc cggaagtcct atactacgaa caatcaacat gggacagttg
      421 ccattataga caacaaatat atcaagcttc ctaagattgg caaagtaaaa gccgtcatcc
      481 atcgcattcc tgatagcgat tggattgtaa aatcagctac ggtatcacag gaatcagatg
      541 gcaaatatta tatttctgtt ctctttgagt ttgacaatgc cataaatctt tatgtagcag
      601 ataagaccaa tgctatcgga ttagattata cttctaatgg cttgtatgtg gacagtaacg
      661 gtaacgtagg aacaaatcac aaatactatc gtgaaagcca tgacaaactt gccaaggcac
      721 aacgtaggct gtcacgtatg caaggctcta aaaagcatga aatcaaatcc aacaattata
      781 taaaacaact tcgcaaagtg aataagatac ataggtatat cgctaatcag cgtctggata
      841 acctgcataa aatatctacg aagatagcca attcgtatga tgttgtttgc gtggaatcct
      901 taaacatgag gtctatggca aatcatggct tcggtaacgg caaagctact cttgataacg
      961 gttatggtat gttcctgtct atgcttgaat acaagctatc tgataggaat aagtatctta
     1021 tcaaggtaga taagtggttt ccgtcatcac aaatatgtca ttgttgtgga aagatacatc
     1081 ccgagatgaa aaatctcaca attcgcacaa tgaaatgtga ttgtggtctt acgatgaatc
     1141 gtgaccagaa tgccgctatc aatatcttac gtgagggatt acgactactg actaaagtag
     1201 cctgagagat ctttgacagc tagctcagtc ctaggtataa tagaatgccg ctatcaatat
     1261 cttacgtgag ggattacgac tactgactaa agtagcctaa aactaaatat tacagtaggg
     1321 atggaatagc ccaaactcat acgcctgtgg acactgtgta agacgagccg ttacgtatca
     1381 tcgtagccag cgcagtagtg gttgaagcag gaagctcggt tacttgtaac cgagtagttc
     1441 acgctcggag atcatcattg cgttttttaa gcttggctgt tttggcggat gagagaagat
     1501 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
     1561 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
     1621 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
     1681 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1741 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
     1801 ccggagggtg ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
     1861 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat
     1921 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1981 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     2041 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     2101 tcagttgggg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2161 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2221 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2281 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2341 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2401 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttacgc gccctgtagc
     2461 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     2521 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     2581 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     2641 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     2701 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     2761 acttgaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     2821 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     2881 aaaatattaa cgtttacaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2941 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3001 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3061 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3121 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3181 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3241 accagtcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
     3301 aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg
     3361 aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc
     3421 aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc
     3481 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac agacggcatg
     3541 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     3601 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     3661 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     3721 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     3781 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     3841 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa
     3901 ctccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     3961 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     4021 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     4081 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     4141 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     4201 acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt
     4261 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta
     4321 ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt
     4381 tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg
     4441 gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc
     4501 ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag
     4561 gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact
     4621 gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga
     4681 gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc
     4741 cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt
     4801 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg
     4861 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt
     4921 tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt
     4981 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5041 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
     5101 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
     5161 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
     5221 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
     5281 acgatctcaa gaagatcatc ttattaatca gataaaatat ttctaggctc atgagcccga
     5341 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     5401 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga
     5461 cagcttatc     
//
