LOCUS       pCBad33_ISCytsp1        5446 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    N11 mpCBad33-pISN11(Cytsp1)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    69..1178
                     /note="ISCytsp1"
                     /note="color: #00ff00"
                     /locus_tag="ISCytsp1"
                     /label=ISCytsp1
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1189..1218
                     /note="J23119 promoter "
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffc0cb"
                     /locus_tag="J23119 promoter"
                     /label=J23119 promoter
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1420..1439
                     /note="gRGS"
                     /note="color: #99ccff"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1440..1445
                     /note="PolyT terminator"
                     /note="color: #d6b295"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3434..4093)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4455..5371
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1219..1419
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaat gactaaacaa aataaagcgt acaagttcag gttgtatcca acagatgaac
      121 aagcttttat tatacgaaaa acctttgggt gtgttcgctt cgtctacaat aaaatgttag
      181 ctgaacgaat agaaacgtat gaaagcctaa aagaagataa agaagcattg aaaaagataa
      241 agcacccaac tcctgctaag tataaaaaag agtatgaatg gctaaaagaa gtagactctt
      301 tagcattagc gaacgcacaa ctaaacttag ataaggcata taaagccttc ttcaaaggga
      361 atgtcaagtt tccaacgttc aaaaggaaac gacacaaaca aagctacaca acaaatgttg
      421 tcaatggaaa tattcagttg ttggatggtc atatcaaatt acctaagttg aaattggtca
      481 aaattaaaca acatcgaaag ataccttcag aatacaaaat caaatcctgc acattatcta
      541 tgaccgcatc aggaaaatat tatatttcta ttttggcaga gtacgaggtg gaaattgaaa
      601 ggaaggaagt taaaaaagtc gttgggttag attttgcgat ggatggattg tttgtggata
      661 gcgagggtga gaaagccaat taccctaagt tttatcgaca aaaacttgaa aaattagcaa
      721 aagaacaacg taaactgtct cgtaagaaga aaggttcttc caattggaat aaacaacgta
      781 ttcgagtagc taacattcaa gagaaagtcg ccaatcaacg gaagaacttt cttcaccatc
      841 aatcaaaaaa attagtagcg aactatgatg ctgttgtgat tgaagattta gatatgaaag
      901 gaatgtcaca agcacttaaa ttcggtaaaa gtgtagctga taatggatgg ggaatgttca
      961 cttctttctt acagtacaaa ctaaaagaac aagggaaaca actgatcaaa atagacaaat
     1021 ggttcccctc tacccaaacc tgttcgcatt gtggaaatac ccaactaatg ccaatgaaca
     1081 tcagaatata tgtttgttcg tgcggactaa acctcgatag agattacaat tcagcactca
     1141 atatcaaaaa agaaggcatt cgcttattag caagtgcctg agagatcttt gacagctagc
     1201 tcagtcctag gtataataga tagagattac aattcagcac tcaatatcaa aaaagaaggc
     1261 attcgcttat tagcaagtgc ctaaagaaat aagagatata aactcttgga acaagagggt
     1321 tagcttggtc aatttcgtca gctaccaaaa gtggcgatta cccaagaagc ccccacttca
     1381 agcaacccgc aagggtggta agtggtggga gtagttcacg ctcggagatc atcattgcgt
     1441 tttttaagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat
     1501 cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc
     1561 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
     1621 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa
     1681 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     1741 ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtgggc gggcaggacg
     1801 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     1861 tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1921 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1981 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2041 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttggggcaa actattaact
     2101 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2161 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2221 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2281 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2341 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2401 tcatatatac tttagattga tttacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     2461 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     2521 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     2581 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     2641 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     2701 ggagtccacg ttctttaata gtggactctt gttccaaact tgaacaacac tcaaccctat
     2761 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     2821 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaattta
     2881 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3121 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtcaggcat ttgagaagca
     3241 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc
     3301 tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca
     3361 ttcatccgct tattatcact tattcaggcg tagcaccagg cgtttaaggg caccaataac
     3421 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     3481 gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca
     3541 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     3601 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     3661 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     3721 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     3781 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     3841 atatcaccag ctcaccgtct ttcattgcca tacggaactc cggatgagca ttcatcaggc
     3901 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     3961 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     4021 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     4081 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     4141 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     4201 ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta
     4261 tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg
     4321 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct
     4381 gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc
     4441 accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg
     4501 gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa
     4561 tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     4621 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa
     4681 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc
     4741 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
     4801 taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
     4861 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
     4921 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     4981 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
     5041 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
     5101 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
     5161 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
     5221 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
     5281 ttaatcagat aaaatatttc taggctcatg agcccgaagt ggcgagcccg atcttcccca
     5341 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     5401 acgatgcgtc cggcgtagag gatctgctca tgtttgacag cttatc 
//
