LOCUS       pCBad33_pISEc41_        5583 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    49 mpCBad33-dIS49(Ec41)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    81..1283
                     /note="ISEc41"
                     /note="color: #00ff00"
                     /locus_tag="ISEc41"
                     /label=ISEc41
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1300..1334
                     /note="J23119 promoter modified to end with an SpeI si"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffc0cb"
                     /locus_tag="J23119 promoter modified to end with an SpeI
                     site"
                     /label=J23119 promoter modified to end with an SpeI site
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1557..1576
                     /note="gRGS"
                     /note="color: #a6acb3"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1577..1582
                     /note="PolyT terminator"
                     /note="color: #d6b295"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3571..4230)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4592..5508
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1335..1556
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaga attcgaattc atgttaatcc agaaagcata taaatacagg ctctacccca
      121 ctgaccagca ggcgcaaagg ttgcgtcagt tgtgcggttg cgctcgcttt gtctggaact
      181 atgcactgaa tgaaacactg agcattcatg acgcgggcgg aaaaattccg tcagctttcg
      241 acctgaataa acgacttacg ggctggaaaa aactccctga gctggccttt ctttctgagg
      301 gttacactga caacctccag cagaaactga aagacctgcg gagcgcatgg gatcgctgtt
      361 ttgataaatc cctgacggct gaaaagcccg ttttcaaaaa gaaaaccaaa ggctgcgact
      421 ctatccggtt cgtcaatttc agcaaatatt gtgggctgga ttatggccgt gtgaaactcc
      481 cgtcaggact gggctgggtt aaatttcgtc agtcccggaa gattgaaggc gtcattaaaa
      541 attgcaccat cagccagcat gccggacact ggtatgtgtc ttttcaggtc gaattagctg
      601 ttaccgatcc gattcacgca tcgaccagcg caataggcct ggatgcaggt ataacgaagc
      661 tggcaacact ttcagacggc acggtctttg aaccggtaaa cagcttaaaa aaaaatcagg
      721 ataagctggc tcgcttacag cgccgtctgg cccggatggt taaattctcc gccaactgga
      781 aaaagcagaa agcgaagatt tcccgtttcc actctcatat cgcaaacatc cgccgcgatt
      841 accttcataa aaccacgacg acgatcagca aaaaccacgc gatgatagtc attgaggatt
      901 taaaggtttc gaacatgtca aaatcagcag cgggtacggt agaccagccg gggcgcaacg
      961 tcgcggcaaa atcaggtctt aaccgggcga tactggatca gggctgggca gaaatgcgcc
     1021 gccagctcga atataagcag gcatggcgcg gcggtgatgt actggcgatt aatccggcgt
     1081 acaccagtca gaaatgcgca tgttgtgggc atacatcgaa aaataaccgc aggacgcagg
     1141 cgagctttat ctgtaccgcg tgcggctata cggcaaacgc cgatgtaaac ggcgctcgca
     1201 atatattaac agcaggtttt gaagtcatgg cggcaggaca aatattgccg tcagtccgta
     1261 aaggacgcgc ccgtaaggcg gcgggatcct gagagatctt tgacagctag ctcagtccta
     1321 ggtataatac tagtctatac ggcaaacgcc gatgtaaacg gcgctcgcaa tatattaaca
     1381 gcaggttttg aagtcatggc ggcaggacaa atattgccgt cagtccgtaa aggacgcgcc
     1441 cgtaaggcgg cgtaagccgt cgagcgagga accggggaat gcggcaacgt attgctaccc
     1501 tgacaaggga atccccctcc tgagtcgctt tgcgcgaagg tgggggagga tgtcaagctc
     1561 ggagatcatc attgcgtttt ttaagcttgg ctgttttggc ggatgagaga agattttcag
     1621 cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg
     1681 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc
     1741 cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac
     1801 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1861 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
     1921 ggtgggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc
     1981 ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc
     2041 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2101 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     2161 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2221 ggggcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2281 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2341 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2401 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2461 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2521 tgtcagacca agtttactca tatatacttt agattgattt acgcgccctg tagcggcgca
     2581 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2641 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2701 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2761 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2821 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaacttga
     2881 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     2941 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3001 ttaacgttta caatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3061 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3121 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3181 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3241 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3301 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3361 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     3421 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg
     3481 ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt
     3541 ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta
     3601 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac
     3661 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     3721 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     3781 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     3841 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     3901 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     3961 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaactccgg
     4021 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     4081 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     4141 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     4201 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     4261 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     4321 tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac
     4381 agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc
     4441 gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag
     4501 gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg
     4561 tgcgtaacgg caaaagcacc gccggacatc agcgctagcg gagtgtatac tggcttacta
     4621 tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca
     4681 ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg
     4741 ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc
     4801 ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt
     4861 tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc
     4921 gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct
     4981 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
     5041 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
     5101 gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5161 ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt
     5221 agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc
     5281 tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc
     5341 gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc
     5401 tcaagaagat catcttatta atcagataaa atatttctag gctcatgagc ccgaagtggc
     5461 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     5521 cgccggtgat gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt
     5581 atc     
//
