LOCUS       pCBad33_pISKpn69        5558 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    34 mpCBad33-pIS34(Kpn69)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #a6acb3"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #a6acb3"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    75..1259
                     /note="ISKpn69"
                     /note="color: #a6acb3"
                     /locus_tag="ISKpn69"
                     /label=ISKpn69
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1276..1310
                     /note="J23119 promoter modified to end with an SpeI si"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffffff"
                     /locus_tag="J23119 promoter modified to end with an SpeI
                     site"
                     /label=J23119 promoter modified to end with an SpeI site
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1532..1551
                     /note="gRGS"
                     /note="color: #a6acb3"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1552..1557
                     /note="PolyT terminator"
                     /note="color: #a6acb3"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3546..4205)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #a6acb3; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4567..5483
                     /note="p15a ori"
                     /note="color: #a6acb3"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1311..1531
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaga attcatgcta atccagaaag cgtataaata cagactcaat ccgacagatg
      121 cacaggcgca acgactgcgt cagatgtgcg gctgcgcccg ttttgtctgg aattacgccc
      181 tggcagagac gcagcgtatt cttgaggccg gacaaaaact cccttccgcc tttgagctga
      241 ataaaatgct gacaggatgg aagaaattac ctgacctggc gtttttatct gatgcctaca
      301 ccgacaacct tcagcagaaa ctgaaagacc tgcacggggc atggatacgg tgctttgata
      361 aaaaactggc agcgaaagcg cccgtgttca aacgtaaaac ccacggcaat gattccgttc
      421 gtttcgtcaa ttttgacaaa tactgccagc ttgagtacag acgcgttaag ttaccctcgg
      481 ggctgggctg ggtgacattc cggcagtccc gggccgttgt gggtaaaatc aaaaacgcaa
      541 cggtcagcca gcacgctggc ggctggtata tctcgtttca ggtagaaatg gaggtcgaac
      601 agcccgcgca cccgtcaacg tctgctgtcg gcctggatgc cggtatctgc aagctggtaa
      661 cgctgtcaga cggcacggtg tttgaacctg tcaacagttt taaacagaac caaagcagac
      721 tggcaaggtt acagcgccag cttgcccgta aagtgaaatt cagtgcgaac tggaagaagc
      781 agaaagcaag aatttcccgc ttacattcgc atatcgccaa catccgccgc gattaccttc
      841 acaaagtcac gacaacaatc agcaaaaacc acgcgatgat cgttattgaa gatttgaagg
      901 tttccaacat gtcgaaatcg gcagcgggta cggtagacga gccggggcgc aacgttgcgg
      961 caaaatcagg tcttaaccgg gcgatactgg atcagggctg gtacgaaatg cgccgccagc
     1021 tcgaatataa gcagcgatgg cgcggcggtg aggtgctggc agtaaatccg gcatatacca
     1081 gccagaaatg cgcgcgttgc ggtcatacag taaaggaaaa tcgtcaatcg caggcagtat
     1141 ttgtctgcgt ggcgtgtgga tacgaagcga acgccgacat aaacggcgct caaaatattc
     1201 tcgcggcagg acatgccgtg ttgtccggga ttaaccccgg acgcgcccgt aaggcggcgg
     1261 gatcctgaga gatctttgac agctagctca gtcctaggta taatactagt gtctgcgtgg
     1321 cgtgtggata cgaagcgaac gccgacataa acggcgctca aaatattctc gcggcaggac
     1381 atgccgtgtt gtccgggatt aaccccggac gcgcccgtaa ggcggcgtaa gccgttaagc
     1441 gaggaaccgg gcaatgcggt aacgcgttgc taccctacaa agggaatccc cttcctgatt
     1501 cacgtagtga gaaggtgggg gaggatgtca agctcggaga tcatcattgc gttttttaag
     1561 cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc
     1621 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac
     1681 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat
     1741 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1801 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
     1861 agcggatttg aacgttgcga agcaacggcc cggagggtgg gcgggcagga cgcccgccat
     1921 aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc
     1981 tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2041 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2101 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2161 acgctggtga aagtaaaaga tgctgaagat cagttggggc aaactattaa ctggcgaact
     2221 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     2281 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2341 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2401 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2461 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2521 actttagatt gatttacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2581 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     2641 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2701 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     2761 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     2821 cgttctttaa tagtggactc ttgttccaaa cttgaacaac actcaaccct atctcgggct
     2881 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     2941 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt taaaaggatc
     3001 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3061 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3121 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3181 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3241 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3301 cctacatacc tcgctctgct aatcctgtta ccagtcaggc atttgagaag cacacggtca
     3361 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac
     3421 gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg
     3481 cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa
     3541 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     3601 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc
     3661 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     3721 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     3781 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     3841 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     3901 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     3961 agctcaccgt ctttcattgc catacggaac tccggatgag cattcatcag gcgggcaaga
     4021 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     4081 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     4141 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     4201 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     4261 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
     4321 cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct
     4381 gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc
     4441 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
     4501 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
     4561 acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt
     4621 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat
     4681 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     4741 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     4801 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     4861 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     4921 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     4981 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     5041 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct
     5101 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac
     5161 tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa
     5221 ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc
     5281 aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt
     5341 tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag
     5401 ataaaatatt tctaggctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     5461 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     5521 tccggcgtag aggatctgct catgtttgac agcttatc  
//
