LOCUS       pCBad33_pISMmu1_        5759 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    63 mpCBad33-pIS63(Mmu1)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    75..1376
                     /note="ISMmu1"
                     /note="color: #00ff00"
                     /locus_tag="ISMmu1"
                     /label=ISMmu1
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1393..1427
                     /note="J23119 promoter modified to end with an SpeI si"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffc0cb"
                     /locus_tag="J23119 promoter modified to end with an SpeI
                     site"
                     /label=J23119 promoter modified to end with an SpeI site
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1733..1752
                     /note="gRGS"
                     /note="color: #a6acb3"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1753..1758
                     /note="PolyT terminator"
                     /note="color: #d6b295"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3747..4406)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4768..5684
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1428..1732
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agacctaaag
       61 aggagaaaga attcatgctg tataatagaa gtaggaagga ggcgaataaa atggaacata
      121 agaagatggc ggattatgcc tatcattacg gtctgaaagt ccgcatctac cccagccggc
      181 aacagaaaga gatgattcga cgcaatgcgg atgcggcacg ctgcatctac aatgaacatg
      241 tcgctgtgaa caaggagatc gcacgattcg gcagacggag catctacatc gacttcattg
      301 agaagcgcat cgaggcactg cgcgagcgaa tccgcacggt aacagcattg aaagaccgct
      361 atccatggct ccggcatccc gatatcgatg cgcagtccat tgccaatgca cggcagaact
      421 accagcatgc ttggaagcag ttccgtgccg tccatcatgc tggtgtgccg aacttccaca
      481 agaaaacgtc cgtcgagtcg tatcagacga atccgcatta cagcaagggt acggtgattt
      541 cgctgtttac cagtaacgcc cgcttcctcg acaagcatca tctgatgctg cccaagctca
      601 aacgcatccg cttccaaggt tcgcgtaaaa tactggaccg actttttgcc atgaaggaag
      661 tccgcatcgg aacgattacc atcacgaaag acagcctgca ccgctacttt gcgtcattcc
      721 aactcggctc cgatgaacca ttcgtgaaag ccagcccgaa aacaaaggca gagattggca
      781 tcgacctgaa catcgagaac ttctatgccg actcgaatgg cgagattgtg gccaatcctc
      841 gttattaccg caggcagaaa cgacggctgg ccaacgcaca gcgcaagctg agccgtcgga
      901 ttgtccgtgc caagaaagag catcgctcgc tccgtgaggc aaagaacgtc gaaaaacagc
      961 gcttgctcgt cgccaggctc acggcacaag tcatgcagcg ccgcaaagat ttcctgcacc
     1021 gcacttccgc gacactgatc aagaaccacg atctggtcgc agcagaagaa ctacggagca
     1081 agaatctgct gaagaaccat gccctggcta tgagcatcca agacaatggc tggcggatgt
     1141 tccttagtat gctgacttat aaggcagcac tgtacggcaa gaacttcgtc accgtcgacc
     1201 cgcggaatac gacgcaaacc tgccacgctt gtggccatat catgagcggt aaagagaaac
     1261 tgacgctcaa ggaccgcgag tggatctgcc cgaaatgtgg gatacaccac cttcgcgatg
     1321 tgaatgcggc caagaacatc ctagcgcgag ccaaagcaaa gctcgtatcc atgcatggat
     1381 cctgagagat ctttgacagc tagctcagtc ctaggtataa tactagtgcg gtaaagagaa
     1441 actgacgctc aaggaccgcg agtggatctg cccgaaatgt gggatacacc accttcgcga
     1501 tgtgaatgcg gccaagaaca tcctagcgcg agccaaagca aagctcgtat ccatgcattg
     1561 atgcgaacct accttgtccg tttggcaact tgcggacgct aatggctttg gtaattaccg
     1621 gacctctgct gtacctcgca agactgcgaa tgcagtaagc ctggcctatc taagcaaatt
     1681 gaggtatcga aagattccct caagctcgcg acttcagtcg cgggtagttg acgctcggag
     1741 atcatcattg cgttttttaa gcttggctgt tttggcggat gagagaagat tttcagcctg
     1801 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
     1861 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
     1921 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
     1981 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     2041 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     2101 ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga
     2161 cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat
     2221 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2281 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     2341 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggg
     2401 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2461 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2521 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2581 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2641 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2701 agaccaagtt tactcatata tactttagat tgatttacgc gccctgtagc ggcgcattaa
     2761 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2821 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2881 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2941 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3001 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa acttgaacaa
     3061 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     3121 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3181 cgtttacaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3241 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3301 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3361 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3421 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3481 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtcagg
     3541 catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat
     3601 agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt
     3661 cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa
     3721 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt
     3781 tgtaattcat taagcattct gccgacatgg aagccatcac agacggcatg atgaacctga
     3841 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     3901 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     3961 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     4021 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     4081 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     4141 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ctccggatga
     4201 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     4261 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     4321 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     4381 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     4441 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     4501 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     4561 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa
     4621 agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     4681 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
     4741 taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt
     4801 ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg
     4861 tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac
     4921 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     4981 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     5041 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     5101 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     5161 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     5221 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     5281 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5341 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     5401 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     5461 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     5521 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     5581 gaagatcatc ttattaatca gataaaatat ttctaggctc atgagcccga agtggcgagc
     5641 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     5701 ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatc
//
