LOCUS       pCBad33_pISNsp2_        5476 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    47 mpCBad33-pIS47(Nsp2)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #a6acb3"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #a6acb3"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    81..1202
                     /note="ISNsp2"
                     /note="color: #a6acb3"
                     /locus_tag="ISNsp2"
                     /label=ISNsp2
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1219..1253
                     /note="J23119 promoter modified to end with an SpeI si"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffffff"
                     /locus_tag="J23119 promoter modified to end with an SpeI
                     site"
                     /label=J23119 promoter modified to end with an SpeI site
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1450..1469
                     /note="gRGS"
                     /note="color: #a6acb3"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1470..1475
                     /note="PolyT terminator"
                     /note="color: #a6acb3"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3464..4123)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #a6acb3; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4485..5401
                     /note="p15a ori"
                     /note="color: #a6acb3"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1254..1449
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaga attcgaattc atgaaaacct tgaagttcaa actgtaccag cacaaaagaa
      121 atagatttct caaacgcatg attaatgcag gtggggcaat ctacaaccat tgtattgctc
      181 tacataaacg ctactatcgt atgtggggca aacacttaaa ctgtgcaaaa cttcaagctc
      241 atattgccaa actgagaaag cgtaacccat tttggcaaac agtaggttct caggcggtac
      301 aagatatttg tcaacgcatt gagaaagcct accaattatt ttttaaacac aacaagaagg
      361 gggttagacc accaggattt aagaaggtaa aaaaatacaa atcatttact ctaaaacaag
      421 caggttataa gtttttggat ggaaatagag taaaaattgg gagtaaaatt tatcagtttt
      481 ggaagtctag agaaattgag gggacagtca aaacattaac tattaaacgc acaccattag
      541 gtgagttgtt tatggttgtg gtggttgacg aaggtaacaa gccagaaacc aaattcatga
      601 ctggtaagat agcgggattt gatttcggtt taaaaacctt tctcacttgc tcagacggta
      661 caacaattga gtctccccag ttttttaagc aatccctaaa tgcaattaaa aaagccagcc
      721 gacagcattc taagaaatta aaaggttcag ccaatcgtga gcaagctaga aagaacttag
      781 tacgcaggta tgaagatatc tttaaccgtc gtcgtgattg gttctggaag ttagcccatg
      841 agttaacgga taaatttgat gttctctgtt ttgaaacctt aaaccttaaa ggaatgcaac
      901 gactttgggg aaggaaaata tcagacttgg cttttggtga attactgcaa attctagaat
      961 gggttgccaa aaacaagaat aaactggttg ttttcattga cccgtggtat cccagtagca
     1021 aaacctgctc tagctgtgga catattttag aaaacctaga tttgtcaatt agacgctggc
     1081 gttgtccttc atgtcaatct gaaaatgatc gggatggaaa cgctagtttg aatattaaaa
     1141 gagtcgggag ttcgactcta ggcgtaggag atgttagaca gtcccagact gcaatctctg
     1201 tcggatcctg agagatcttt gacagctagc tcagtcctag gtataatact agtgaaaacc
     1261 tagatttgtc aattagacgc tggcgttgtc cttcatgtca atctgaaaat gatcgggatg
     1321 gaaacgctag tttgaatatt aaaagagtcg ggagttcgac tctaggcgta ggagatgtta
     1381 gacagtccca gactgcaatc tctgtctgat gcctgaatcc cccgttttca aaacggggga
     1441 gtggctcaag ctcggagatc atcattgcgt tttttaagct tggctgtttt ggcggatgag
     1501 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     1561 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
     1621 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
     1681 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1741 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
     1801 caacggcccg gagggtgggc gggcaggacg cccgccataa actgccaggc atcaaattaa
     1861 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
     1921 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1981 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2041 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     2101 ctgaagatca gttggggcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2161 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2221 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2281 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2341 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2401 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc
     2461 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2521 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2581 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2641 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     2701 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2761 gttccaaact tgaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     2821 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2881 ttttaacaaa atattaacgt ttacaattta aaaggatcta ggtgaagatc ctttttgata
     2941 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3001 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3061 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3121 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     3181 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3241 tcctgttacc agtcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
     3301 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac
     3361 cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg
     3421 tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc
     3481 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga
     3541 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     3601 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     3661 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     3721 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     3781 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     3841 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     3901 tacggaactc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     3961 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     4021 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     4081 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     4141 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     4201 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct
     4261 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag
     4321 gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga
     4381 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct
     4441 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta
     4501 tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag
     4561 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc
     4621 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
     4681 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
     4741 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     4801 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
     4861 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     4921 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     4981 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
     5041 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
     5101 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
     5161 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
     5221 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
     5281 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc taggctcatg
     5341 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     5401 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctgctca
     5461 tgtttgacag cttatc    
//
