LOCUS       pCBad33_pISNsp3_        5493 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    18 mpCBad33-pIS18(Nsp3)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    75..1196
                     /note="ISNsp3"
                     /note="color: #00ff00"
                     /locus_tag="ISNsp3"
                     /label=ISNsp3
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1213..1247
                     /note="J23119 promoter modified to end with an SpeI si"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ff99cc"
                     /locus_tag="J23119 promoter modified to end with an SpeI
                     site"
                     /label=J23119 promoter modified to end with an SpeI site
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1467..1486
                     /note="gRGS"
                     /note="color: #a6acb3"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1487..1492
                     /note="PolyT terminator"
                     /note="color: #ffcc99"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3481..4140)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4502..5418
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1248..1466
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaga attcatgaaa accttgaagt tcaaactgta ccagcacaaa agaaatagat
      121 tcctcaagcg catgattaat gcgagtgggg taatctacaa ccattgtatt gccctacaca
      181 aacgctacta ccgaatgtgg ggcaaacact taagttgtgc aaaacttcaa gctcacattg
      241 ctaaactgcg gaagcgtaac ccattttggc aaactgtagg atctcaggca gtgcaagata
      301 tctgtcaacg cattgaaaaa gcttaccaat tattttttaa acacaataag aagggggtta
      361 gaccaccagg atttaagaag gtgaagaaat ataaatcctt caccctaaaa caagcaggtt
      421 ataagttttt aggtggcaac agagtaaaaa tcggtaatcg agtttatcaa ttctggaagt
      481 cgagacagat agagggaaca gttaaaaccc taactataaa gcgcacaccg ttaggtgagt
      541 tgttcgtggt tgtggtagtt gacgacggta ttaagccaga aatcgagatt aagacgggta
      601 aaatcgctgg ttttgacttt ggtttgaaga catttctcac ttgctcagac ggcacaaaaa
      661 ttgattctcc tcagtttttc aaacaatctc taaacgctat caccaaagct agtagacaac
      721 actcaaagaa actcaaaggt tcggctaatc ggaaacgagc gagacttaat ctagtacgca
      781 agtacgaaga tatttctaat cgccgtcgtg actggttctg gaaactagct tatgagttaa
      841 cagataagtt tgatgttctc tgctttgaaa ccttaaacct caagggaatg caacgacgtt
      901 ggggcaggaa aatatcagat ttggcgtttg gtgagtttct ggaaattcta gaatgggttg
      961 ccaaaaagaa gaataaactg attgtcttca ttgatcagtg gtatcccagc actaagacct
     1021 gttctcactg tggtcatgtc ttagagagta ttgatttgtc tgttcggtcg tggagttgtc
     1081 cgtcctgcca gtcagtgaac gagagagacg aaaacgcctc taaggtaatt tgtgcagtcg
     1141 gggcatcgac tgttgggtta ggtgatgtca gactggctac gccagcaatc gctgttggat
     1201 cctgagagat ctttgacagc tagctcagtc ctaggtataa tactagtgtt ctcactgtgg
     1261 tcatgtctta gagagtattg atttgtctgt tcggtcgtgg agttgtccgt cctgccagtc
     1321 agtgaacgag agagacgaaa acgcctctaa ggtaatttgt gcagtcgggg catcgactgt
     1381 tgggttaggt gatgtcagac tggctacgcc agcaatcgct gtttgagcct agaatccccc
     1441 gaattatatt cgggggagta tgtcaagctc ggagatcatc attgcgtttt ttaagcttgg
     1501 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
     1561 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
     1621 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
     1681 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1741 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
     1801 atttgaacgt tgcgaagcaa cggcccggag ggtgggcggg caggacgccc gccataaact
     1861 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
     1921 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1981 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     2041 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     2101 ggtgaaagta aaagatgctg aagatcagtt ggggcaaact attaactggc gaactactta
     2161 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     2221 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     2281 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     2341 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     2401 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     2461 agattgattt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2521 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2581 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2641 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     2701 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2761 tttaatagtg gactcttgtt ccaaacttga acaacactca accctatctc gggctattct
     2821 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2881 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaaa ggatctaggt
     2941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3181 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3241 atacctcgct ctgctaatcc tgttaccagt caggcatttg agaagcacac ggtcacactg
     3301 cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc
     3361 tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat
     3421 tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa
     3481 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     3541 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     3601 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     3661 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     3721 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     3781 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     3841 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     3901 accgtctttc attgccatac ggaactccgg atgagcattc atcaggcggg caagaatgtg
     3961 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     4021 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     4081 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     4141 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     4201 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
     4261 aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa
     4321 gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt
     4381 actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct
     4441 gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc
     4501 agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt
     4561 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg
     4621 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg
     4681 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg
     4741 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc
     4801 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
     4861 cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
     4921 tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag
     4981 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc
     5041 ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca
     5101 gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta
     5161 aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga
     5221 gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga
     5281 gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa
     5341 atatttctag gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     5401 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     5461 cgtagaggat ctgctcatgt ttgacagctt atc  
//
