LOCUS       pCBad33_pISTfu1_        5562 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    9 mpCBad33-pIS9(Tfu1)-sgBbsI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    75..1265
                     /note="ISTfu1"
                     /note="color: #00ff00"
                     /locus_tag="ISTfu1"
                     /label=ISTfu1
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1282..1316
                     /note="J23119 promoter modified to end with an SpeI si"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ff99cc"
                     /locus_tag="J23119 promoter modified to end with an SpeI
                     site"
                     /label=J23119 promoter modified to end with an SpeI site
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1536..1555
                     /note="gRGS"
                     /note="color: #a6acb3"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1556..1561
                     /note="PolyT terminator"
                     /note="color: #ffcc99"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3550..4209)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4571..5487
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1317..1535
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaga attcatgctg accggacgcc gctacctgct ggctttcacc cccgagcagg
      121 aggagttcgc cgagaagatc ggtgacgcct gccgggttgt gtggaacacc gcgctggagc
      181 agcgccgaca gtaccggcgg cgcggcgcgt tcatcggtta cgtagagcag gcgcggcaga
      241 tggccgaggc caagaaggac ttcccgtggc tggccgaggc cccgtcgcac acgcttcagc
      301 aaacgctccg cgaccttgag cgggcatgca agacgcacgg cacgttcaag gtccgctggc
      361 gctccaagcg gaaaaacgcg ccgacgttcc gcttccccga tcccaagcag atcaccgtgg
      421 agcgaatctc ccgtcgctgg ggccgggtca agctgcccaa gctgggctgg acccgcttcc
      481 ggtggacacg cccgctcggc gggcaacttc gcaatgccac cgtgctcaag gacggtgggc
      541 gctggtacat ctccttctgc gtcgaagacg ggctgatgga gtccgcaccg aatggcaaac
      601 ccccggtggg ggtggatcgt ggtgtcactg tcgcagtcgc cacctcgtcc gggtggatgc
      661 gtgaccgcga gttcgtcacc ccaggggaag cggtacggct caagcgtctt cagcagcagc
      721 tcgcccggca acggaagggc tccaaccggc gcgcggccac ccgggcgaag ctgaccaagc
      781 tgaacgcccg catccgcgcc cggcgtaccg acttcctggc gtggaccgcc aaccgcctca
      841 cctgtgacca cggactggtc gttgtggagg acctcaacat ccgaaacatg accgcctctg
      901 ccaaaggcac gcttgccgag ccggggagga acgtcgccgc caaggctggg ctgaaccggg
      961 cgatcctggc caagggatgg ggcggactac tgaccgcctt ggagcacaag gctcgttacc
     1021 acggctcccg tattctgcgg gtgccacctg cgttcacatc gcagacctgc catgcctgcg
     1081 ggcactgcgc gccggacaac cgtgaaagcc aagcggagtt ccggtgccgc gcctgcggcc
     1141 accaggccaa cgccgacgtg aacgccgcga agaacatcct cgccgccggg ctggcggtga
     1201 cagggcgtgg agacctcgcc gttgggcggt ctgtgaagcg ccaaccaccc aaggtagagg
     1261 cagcgggatc ctgagagatc tttgacagct agctcagtcc taggtataat actagtgtga
     1321 aagccaagcg gagttccggt gccgcgcctg cggccaccag gccaacgccg acgtgaacgc
     1381 cgcgaagaac atcctcgccg ccgggctggc ggtgacaggg cgtggagacc tcgccgttgg
     1441 gcggtctgtg aagcgccaac cacccaaggt agaggcagcg tgaacgcgcc tcagccacgg
     1501 gaatcccccg ccttcaggcg tggggaggaa gtcaagctcg gagatcatca ttgcgttttt
     1561 taagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
     1621 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
     1681 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
     1741 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1801 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     1861 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtgggcgggc aggacgcccg
     1921 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     1981 tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2041 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2101 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2161 agaaacgctg gtgaaagtaa aagatgctga agatcagttg gggcaaacta ttaactggcg
     2221 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2281 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2341 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2401 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2461 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2521 atatacttta gattgattta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2581 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2641 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2701 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     2761 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2821 tccacgttct ttaatagtgg actcttgttc caaacttgaa caacactcaa ccctatctcg
     2881 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     2941 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttaaaag
     3001 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3061 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3121 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3181 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3241 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3301 accgcctaca tacctcgctc tgctaatcct gttaccagtc aggcatttga gaagcacacg
     3361 gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt
     3421 taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca
     3481 tccgcttatt atcacttatt caggcgtagc accaggcgtt taagggcacc aataactgcc
     3541 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     3601 tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag
     3661 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     3721 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     3781 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     3841 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     3901 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     3961 caccagctca ccgtctttca ttgccatacg gaactccgga tgagcattca tcaggcgggc
     4021 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     4081 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     4141 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     4201 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     4261 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     4321 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
     4381 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc
     4441 tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt
     4501 ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg
     4561 ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt
     4621 cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg
     4681 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
     4741 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata
     4801 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
     4861 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
     4921 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt
     4981 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt
     5041 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
     5101 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca
     5161 ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg
     5221 ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg
     5281 gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc
     5341 gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa
     5401 tcagataaaa tatttctagg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     5461 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     5521 tgcgtccggc gtagaggatc tgctcatgtt tgacagctta tc 
//
