LOCUS       pCBad33_ISTva1_R        5556 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    N4 mpCBad33-pISN4(Tva1)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    69..1289
                     /note="ISTva1"
                     /note="color: #00ff00"
                     /locus_tag="ISTva1"
                     /label=ISTva1
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1300..1329
                     /note="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffc0cb"
                     /locus_tag="J23119 promoter"
                     /label=J23119 promoter
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1530..1549
                     /note="gRGS"
                     /note="color: #99ccff"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1550..1555
                     /note="PolyT terminator"
                     /note="color: #d6b295"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3544..4203)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4565..5481
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1330..1529
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaat gcagagagcg tttaaagtta cactcattcc taatcacaat caagaggtct
      121 taatcaataa gagtattggt tgtgcaaggt ttgtgtataa ccactttctg gcattaaagc
      181 aagagctata tcagtccgaa cagaaaacat taaactataa cgcttgtagt caacgtttaa
      241 ctttactcaa gaaagaaatt gaatggttga aggaagtaga taagtttgcc ttacagaact
      301 cgctcaagaa tttagagaca gcatacaaaa acttttttgc tgacttaaag aaggtcaaag
      361 gcaaaaaagg agtaggcttt cccaagttta aaaagaagca tggttgcaag cagtcttaca
      421 agacgaattt aacaaacggt aacatccagg taatagagaa tcgtttaaag cttccgaagt
      481 tagggtgggt gaagtttcat aagtcccaag aagttaccgg aaagcttata aacgttacca
      541 taactcggac ttcttctggt aaatatgttg ctagtattct gtgtgataca gagattgaga
      601 aacatcccca agttagtcaa aatattggca tagacttagg cataaagtct tatctcgtta
      661 ctagtaatgg ggaagtcgta gataatccca aatattaccg gactcaaact cgtaaattac
      721 gtaaagcaca taaaaaatta tcccgcagtg taaaaggcag tactaatcga gtcaaagcga
      781 aaatcaagct ggctcgtagc tacgaaagaa ttaccaatct cagagatgac tttctgcaca
      841 agctgtcaac tcgtctaatc aaagaaaaca gtattatctg tattgaggat ttgcgagttg
      901 ccaacatggt aaaaaaccat aaattatcat tgagtatttc agacgctagt tggtctaagt
      961 tcgttgccat gcttgaatat aaagcttttt ggcatgacag aattgtgcag aaggttggta
     1021 cgttttatcc ctcctctcag acttgtaatc attgtggttt tatcaaccct ttggtcaaag
     1081 atttaaagtt acgtgaatgg gcttgtcctg gttgtagtgg ttacaatttg agagacaata
     1141 acgcagcgtt gaacatatta ggtgagggat tgagattaat agccgccgtg ggtatcccgg
     1201 aggctctaaa cgcctgtgga gaactcgtca gtcctggagc aattcaggca gagatcgttg
     1261 aagcaggaat cacgcgactt caagtcgtgt gagagatctt tgacagctag ctcagtccta
     1321 ggtataatag gcttgtcctg gttgtagtgg ttacaatttg agagacaata acgcagcgtt
     1381 gaacatatta ggtgagggat tgagattaat agccgccgtg ggtatcccgg aggctctaaa
     1441 cgcctgtgga gaactcgtca gtcctggagc aattcaggca gagatcgttg aagcaggaat
     1501 cacgcgactt caagtcgtgt gaggttcaag ctcggagatc atcattgcgt tttttaagct
     1561 tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
     1621 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
     1681 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
     1741 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1801 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
     1861 cggatttgaa cgttgcgaag caacggcccg gagggtgggc gggcaggacg cccgccataa
     1921 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
     1981 caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2041 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2101 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2161 gctggtgaaa gtaaaagatg ctgaagatca gttggggcaa actattaact ggcgaactac
     2221 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     2281 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     2341 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2401 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2461 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     2521 tttagattga tttacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2581 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     2641 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     2701 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     2761 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     2821 ttctttaata gtggactctt gttccaaact tgaacaacac tcaaccctat ctcgggctat
     2881 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     2941 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaattta aaaggatcta
     3001 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3061 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3121 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3181 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3241 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3301 tacatacctc gctctgctaa tcctgttacc agtcaggcat ttgagaagca cacggtcaca
     3361 ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga
     3421 ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct
     3481 tattatcact tattcaggcg tagcaccagg cgtttaaggg caccaataac tgccttaaaa
     3541 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     3601 gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt
     3661 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     3721 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
     3781 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     3841 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     3901 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     3961 ctcaccgtct ttcattgcca tacggaactc cggatgagca ttcatcaggc gggcaagaat
     4021 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     4081 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     4141 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     4201 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     4261 tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg
     4321 ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc
     4381 gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa
     4441 cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca
     4501 gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc accgccggac
     4561 atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg gtgtcagtga
     4621 agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac
     4681 aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
     4741 gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa gatacttaac
     4801 agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc
     4861 atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
     4921 aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     4981 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
     5041 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
     5101 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
     5161 gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
     5221 ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
     5281 agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
     5341 agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat
     5401 aaaatatttc taggctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     5461 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     5521 cggcgtagag gatctgctca tgtttgacag cttatc  
//
