LOCUS       pCBad33_ISYmu1_R        5485 bp ds-DNA     linear       05-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    N6 mpCBad33-pISN6(Ymu1)-sgRGS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2023Äê7ÔÂ3ÈÕ from SnapGene 2.3.2 http://www.snapgene.com
COMMENT     >pBAD33
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..42
                     /note="J23108 promoter"
                     /note="color: #00ffff"
                     /locus_tag="J23108 promoter"
                     /label=J23108 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    57..68
                     /note="strong bacterial RBS"
                     /note="color: #00ffff"
                     /locus_tag="strong bacterial RBS"
                     /label=strong bacterial RBS
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    69..1214
                     /note="ISYmu1"
                     /note="color: #00ff00"
                     /locus_tag="ISYmu1"
                     /label=ISYmu1
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1225..1254
                     /note="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
                     /note="color: #ffc0cb"
                     /locus_tag="J23119 promoter"
                     /label=J23119 promoter
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1459..1478
                     /note="gRGS"
                     /note="color: #a6acb3"
                     /locus_tag="gRGS"
                     /label=RGS target
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1479..1484
                     /note="PolyT terminator"
                     /note="color: #d6b295"
                     /locus_tag="PolyT terminator"
                     /label=PolyT terminator
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3473..4132)
                     /note="chloramphenicol acetyltransferase"
                     /note="color: #00ff00; direction: LEFT"
                     /locus_tag="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4494..5410
                     /note="p15a ori"
                     /note="color: #00ffff"
                     /locus_tag="p15a ori"
                     /label=p15a ori
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1255..1458
                     /locus_tag="reRNA_bb"
                     /label=reRNA_bb
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcgatctga cagctagctc agtcctaggt ataatgctag ccccgggaaa agatctaaag
       61 aggagaaaat gctgcagcat aaagcctatg aataccgtat ctatccagat aagaagcagg
      121 aaacactgat tgccaagaca ataggcagtt caaggtatgt atacaaccat ttcctggaac
      181 tttggaacaa ggaatatgag gagaccggaa aaggtctgac ttattacgca tgctccaaac
      241 ttctgacaaa gcttaagagg gacccggaaa cagtatggct ttgtgaagtg gataagttct
      301 cgctccagaa ctcgcttcgt aacctgtcgg acgccttctc ccgctttttc aaaggacaga
      361 acgagcatcc ccagttcaag agcaagaaga gcccgaggca aagctacacg acacaatata
      421 cgaacaacaa catcgcagtt tccggaaatt gtctgaagct gcctaagctg ggccttgtca
      481 agtttgcaga cagtagggag atgaaaggcc gtatactgaa tgccacagta cgaaggaaat
      541 cgagcgggaa attcttcgta tcgatcctat gcgaagagga gatctgtgaa ttgccaaaga
      601 ctgactcatc tgtcggaatt gacctcggaa tcattgactt tgcggttatg tcagacggaa
      661 gcaggcatga caacaatcat tttaccaggc aaatggaaga aaggcttaga cgggagcagc
      721 gaaagcttgc aaggcgtgca cttgctgcgg agaaaagggg catttccctt tctgaagcca
      781 ggaactatca gaagcaaagg cggaaggtag cgagacttta tgaaaaggtt gcaaaccagc
      841 gaaaagaata tcttaacaaa ctcagtacag agatagtcaa aaaccacgat atcatctgta
      901 tcgaggacct taacgttaag ggcatgatgc gcaatcataa actggcaaaa agcatctctg
      961 atgtatcatg gacgagcctt gtatcgaaac tgcagtacaa ggcttcctgg tatggaaaag
     1021 aagtcatcag gataagcaga tggtttccgt caagtcagat atgctcagag tgcggccaca
     1081 aggacaggaa gaagcctctc catgtaaggg agtggacctg tcctgtttgt catgcccacc
     1141 atgaccgtga cgtcaatgca gcaagaaata tactggctga gggactcagg ataagagctc
     1201 tgactccggg gtcttgagag atctttgaca gctagctcag tcctaggtat aatagtcaat
     1261 gcagcaagaa atatactggc tgagggactc aggataagag ctctgactcc ggggtcttag
     1321 caatacagaa gcaaacagga accgcaggaa ttgcgggggt agcttggtaa acaagagaaa
     1381 cctctgccgg caaagaaata agccggtaag tatgctctgt tcccaagaat ctcgtgactt
     1441 tagtcatgag agtttcaagc tcggagatca tcattgcgtt ttttaagctt ggctgttttg
     1501 gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
     1561 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
     1621 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
     1681 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1741 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     1801 gttgcgaagc aacggcccgg agggtgggcg ggcaggacgc ccgccataaa ctgccaggca
     1861 tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt
     1921 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1981 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2041 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2101 taaaagatgc tgaagatcag ttggggcaaa ctattaactg gcgaactact tactctagct
     2161 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2221 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2281 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2341 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2401 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2461 ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     2521 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     2581 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     2641 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     2701 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     2761 tggactcttg ttccaaactt gaacaacact caaccctatc tcgggctatt cttttgattt
     2821 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     2881 taacgcgaat tttaacaaaa tattaacgtt tacaatttaa aaggatctag gtgaagatcc
     2941 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3001 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3061 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3121 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3181 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3241 ctctgctaat cctgttacca gtcaggcatt tgagaagcac acggtcacac tgcttccggt
     3301 agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga
     3361 accgacgacc gggtcgaatt tgctttcgaa tttctgccat tcatccgctt attatcactt
     3421 attcaggcgt agcaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc
     3481 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     3541 catcacagac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     3601 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     3661 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
     3721 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     3781 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     3841 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     3901 tcattgccat acggaactcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     3961 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     4021 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     4081 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     4141 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     4201 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg
     4261 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
     4321 ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt
     4381 agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc
     4441 tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag
     4501 cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg
     4561 tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc
     4621 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc
     4681 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
     4741 agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc
     4801 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4861 cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
     4921 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
     4981 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
     5041 taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac
     5101 tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa
     5161 ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag
     5221 ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga
     5281 ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct
     5341 aggctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
     5401 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
     5461 atctgctcat gtttgacagc ttatc   
//
