LOCUS       Exported               11685 bp ds-DNA     circular SYN 20-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       Exported                4570 bp ds-DNA    circular SYN 
            03-FEB-2014
            DEFINITION  .
            ACCESSION   .
            VERSION     .
            KEYWORDS    SM33 - AAVS1-MCS donor
            SOURCE      synthetic DNA construct
              ORGANISM  synthetic DNA construct
            REFERENCE   1  (bases 1 to 4570)
              AUTHORS   .
              TITLE     Direct Submission
              JOURNAL   Exported Monday, Feb 03, 2014 from SnapGene 2.2.2
                        http://www.snapgene.com
            COMMENT     This file is created by Vector NTI
                        http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..11685
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    625..1368
                     /label=AAVS L homology
     exon            725..1102
                     /label=exon1
     misc_feature    1255..1368
                     /label=AAVS integration site
     CDS             1379..2052
                     /label=A2UOCE
     exon            1483..1833
                     /label=CBX3 exon 1
                     /note="CBX3 exon 1"
     variation       1834
                     /label=mutant splice donor
                     /note="mutant splice donor"
     variation       1872
                     /label=mutant cryptic splice acceptor
                     /note="mutant cryptic splice acceptor"
     exon            1976..2051
                     /label=CBX3 alt exon 1
                     /note="CBX3 alt exon 1"
     promoter        2054..3232
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     intron          2285..3223
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
     promoter        3249..3267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3358..3367
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     CDS             3364..4494
                     /codon_start=1
                     /label=hCD46
                     /translation="MEPPGRRECPFPSWRFPGLLLAAMVLLLYSFSDACEEPPTFEAME
                     LIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRD
                     PLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCT
                     PPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPE
                     CKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVP
                     KCLKVSTSSTTKSPASSASGPRPTYKPPVSNYPGYPKPEEGILDSLDVWVIAVIVIAIV
                     VGVAVICVVPYRYLQRRKKKGTYLTDETHREVKFTSL"
     CDS             4495..4560
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     misc_feature    4561..5991
                     /label=HLA-G SCT
                     /label=nonstandard type:  misc_featuree
     CDS             5992..6054
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="GSGEGRGSLLTCGDVEENPGP"
     CDS             6055..7023
                     /codon_start=1
                     /label=hCD47
                     /translation="MWPLVAALLLGSACCGSAQLLFNKTKSVEFTFCNDTVVIPCFVTN
                     MEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSD
                     AVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPIFAILLFWGQFGIK
                     TLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGLGLIVTSTGILIL
                     LHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALA
                     QLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE"
     CDS             7024..7092
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="GSGQCTNYALLKLAGDVESNPGP"
     CDS             7093..7476
                     /codon_start=1
                     /label=hCD59
                     /translation="MGIQGGSVLFGLLLVLAVFCHSGHSLQCYNCPNPTADCKTAVNCS
                     SDFDACLITKAGLQVYNKCWKFEHCNFNDVTTRLRENELTYYCCKKDLCNFNEQLENGG
                     TSLSEKTVLLLVTPFLAAAWSLHP"
     CDS             7477..7551
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="GSGVKQTLNFDLLKLAGDVESNPGP"
     CDS             7552..8346
                     /codon_start=1
                     /label=neomycin resistance
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    8388..8662
                     /label=BGH polyA
     misc_feature    8682..9481
                     /label=AAVS R homology
     primer_bind     complement(9536..9552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9560..9576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thhiogalactopyranoside (IPTG)."
     promoter        complement(9584..9614)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9629..9650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9938..10526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    10700..11557
                     /label=Ampicillin resistance
                     /note="color: #0080ff"
     promoter        complement(11558..11662)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tcgcgccgtg acgtcagcac gccgggcggg gaccgggaga
      661 tccttggggc ggtggggggc cagcggcagt tcccggcggc ccccggggcg ggcgggcggg
      721 cgggtggtgg cggcggttgg ggctcggcgc tcgctcgctc gctgggcggg cgggcggtgc
      781 gatgtccgga gaggatggcc cggcggctgg cccgggggcg gcggcggcgg ctgcccggga
      841 gcggcgacgg gagcagctgc ggcagtgggg ggcgcgggcg ggcgccgagc ctggccccgg
      901 agagcgccgc gcccgcaccg tccgcttcga gcgcgccgcc gagttcctgg cggcctgtgc
      961 gggcggcgac ctggacgagg cgcgtctgat gctgcgcgcc gccgaccctg gccccggcgc
     1021 cgagctcgac cccgccgcgc cgccgcccgc ccgcgccgtg ctggactcca ccaacgccga
     1081 cggtatcagc gccctgcacc aggtcagcgc cccccgcccg gcgtctcccg gggccaggtc
     1141 caccctctgc tgcgccacct ggggcatcct ccttccccgt tgccagtctc gatccgcccc
     1201 gtcgttcctg gccctgggct ttgccaccct atgctgacac cccgtcccag tcccccttac
     1261 cattcccctt cgaccacccc acttccgaat tggagccgct tcaactggcc ctgggcttag
     1321 ccactctgtg ctgaccactc tgccccaggc ctccttacca ttccccttaa acaccggtgg
     1381 gaggtggtcc ctgctgttac gccaatgata acccccgcca gaaaaatctt agtagccttc
     1441 cctttttgtt ttccgtgccc caactcggcg gattgactcg gccccttccg gaaacacccg
     1501 aatcaacttc tagtcaaatt attgttcacg ccgcaatgac ccacccctgg cccgcgtctg
     1561 tggaactgac ccctggtgta caggagagtt cgctgctgaa agtggtccca aaggggtact
     1621 agtttttaag ctcccaactc cccctccccc agcgtctgga ggattccaca ccctcgcacc
     1681 gcaggggcga ggaagtgggc ggagtccggt tttggcgcca gccgctgagg ctgccaagca
     1741 gaaaagccac cgctgaggag actccggtca ctgtcctcgc cccgcctccc ccttccctcc
     1801 ccttggggac caccgggcgc cacgccgcga acgttaagtg ccgcggtcgt cggcgcctcc
     1861 gccctccccc ttgggcccca attcccagcg ggcgcggcgc gcggcccctc cccccgccgg
     1921 gcgcgcgccc gctgccccgc ccttcgtggc cgcccggcgt gggcggtgcc acccctcccc
     1981 ccggcggccc cgcgcgcagc tcccggctcc ctcccccttc ggatgtggct tgagctgtag
     2041 gcgcggaggg ccgggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     2101 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     2161 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     2221 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     2281 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     2341 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     2401 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     2461 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     2521 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     2581 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     2641 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     2701 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     2761 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     2821 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     2881 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     2941 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     3001 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     3061 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     3121 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     3181 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattag
     3241 cttggtacta atacgactca ctatagggag acccaagctg gctaggtaag cttggtaccg
     3301 agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc
     3361 gccatggaac cccctggaag gagagagtgc cctttccctt cctggagatt tcccggactg
     3421 ctgctggctg ccatggtcct gctgctgtac agcttcagcg atgcttgtga ggagcctccc
     3481 acattcgagg ccatggaact cattggcaag cctaagccct actacgaaat cggcgagagg
     3541 gtcgattaca agtgtaagaa gggctatttc tacatccccc ccctggccac acacaccatc
     3601 tgcgatagaa accacacatg gctccctgtg tccgacgatg cttgctatag ggagacctgc
     3661 ccttacatca gggaccctct gaatggacag gccgtgcctg ctaatggcac ctacgaattc
     3721 ggctatcaaa tgcatttcat ctgcaatgag ggctactacc tgatcggcga agagattctc
     3781 tactgtgagc tcaagggaag cgtcgctatc tggagcggaa agcctcccat ctgcgagaaa
     3841 gtgctgtgca cccccccccc taaaatcaaa aatggcaagc acaccttcag cgaggtggaa
     3901 gtgttcgaat atctggatgc cgtgacctac agctgtgatc ctgctcctgg acccgatccc
     3961 tttagcctga ttggcgagag cacaatttac tgcggagaca actccgtgtg gagcagagcc
     4021 gcccccgagt gtaaggtcgt gaaatgtagg ttccctgtcg tcgagaatgg caaacagatc
     4081 agcggcttcg gcaaaaagtt ctactataaa gctaccgtga tgtttgagtg cgacaaggga
     4141 ttctacctcg atggcagcga tacaatcgtg tgcgatagca actccacatg ggacccccct
     4201 gtccccaaat gcctgaaagt gagcaccagc tccacaacca agtcccccgc ctcctccgcc
     4261 tccggcccca gacctaccta taagccccct gtgtccaact accccggcta ccccaaaccc
     4321 gaggaaggca ttctggactc cctggatgtg tgggtgattg ccgtcatcgt cattgctatt
     4381 gtggtcggag tcgctgtgat ctgtgtcgtg ccctatagat acctgcagag aaggaaaaag
     4441 aagggcacct acctgaccga cgagacccac agagaagtga agtttaccag cctgggatcc
     4501 ggagccacca actttagcct gctcaaacaa gccggcgacg tggaagagaa ccccggacct
     4561 atgtccagat ccgtggccct ggctgtgctg gctctgctgt ccctgagcgg cctcgaggcc
     4621 aaaggacccc ccgctgctct gacactgggc tgtggcgctt ccggaggcgg cggcagcgga
     4681 ggcggaggca gcattcagag gacccctaag atccaggtgt actccaggca tcctgccgag
     4741 aacggcaaat ccaatttcct caattgctac gtgtccggct tccatccctc cgacatcgaa
     4801 gtggacctcc tcaagaacgg agagagaatc gaaaaggtgg agcactccga cctgtccttc
     4861 tccaaagact ggtccttcta cctgctgtat tacacagagt ttacccctac cgagaaggac
     4921 gagtacgcct gtagagtgaa tcacgtgaca ctgtcccagc ccaagattgt gaaatgggac
     4981 agggacatgg gcggcggagg aagcggaggc ggcggaagcg gcggcggcgg cagcggcggc
     5041 ggcggctccg gctcccacag catgaggtac ttttccgccg ccgtgtccag gcccggcagg
     5101 ggcgaaccta gatttatcgc tatgggctac gtcgacgaca cccagtttgt gaggttcgac
     5161 agcgactccg cctgccccag aatggaacct agagcccctt gggtcgagca ggaaggcccc
     5221 gagtactggg aggaggagac caggaatacc aaggcccacg ctcagaccga caggatgaat
     5281 ctgcagaccc tgaggggatg ctacaaccaa agcgaggcca gctcccacac cctgcagtgg
     5341 atgatcggct gtgatctggg atccgacggc aggctcctga gaggctatga gcagtacgcc
     5401 tacgatggca aggattacct ggctctcaac gaggacctca gatcctggac agccgccgat
     5461 acagccgctc agatctccaa gaggaagtgc gaggctgcca atgtcgctga gcagaggagg
     5521 gcctacctgg aaggaacctg cgtcgaatgg ctgcacaggt acctggaaaa cggcaaggaa
     5581 atgctccaga gggccgaccc tcctaagaca cacgtcacac accaccccgt gttcgattat
     5641 gaggccacac tcaggtgctg ggctctcggc ttttatcccg ccgagattat cctgacctgg
     5701 cagagagatg gcgaggatca gacccaggac gtcgagctgg tggagacaag gcctgccggc
     5761 gacggaacat ttcagaaatg ggctgctgtg gtggtgccct ccggcgagga acagaggtac
     5821 acatgccacg tgcagcatga aggactcccc gagcccctga tgctgagatg gaagcagtcc
     5881 tccctgccta caatccccat catgggcatc gtggctggcc tcgtcgtcct cgctgctgtg
     5941 gtgaccggcg ctgccgtcgc cgccgtgctg tggagaaaga agtcctccga tggaagcggc
     6001 gagggaaggg gaagcctgct gacatgcggc gatgtggagg agaaccctgg acctatgtgg
     6061 cctctcgtcg ctgccctgct gctgggatcc gcctgctgtg gatccgccca gctcctgttt
     6121 aataaaacaa agagcgtgga gtttaccttt tgtaacgata ccgtggtgat cccctgcttc
     6181 gtcaccaaca tggaagccca gaataccacc gaagtgtatg tcaagtggaa gttcaaaggc
     6241 agggacattt acaccttcga tggcgctctg aacaaaagca ccgtgcccac agatttcagc
     6301 tccgccaaga tcgaagtgtc ccagctgctg aagggcgatg ctagcctgaa aatggacaaa
     6361 tccgacgccg tgagccacac cggcaactat acctgcgagg tcacagaact gacaagggaa
     6421 ggcgagacaa tcatcgagct gaaatacaga gtggtgtcct ggttctcccc taacgagaac
     6481 attctgatcg tgattttccc tatctttgcc attctgctgt tctggggcca gtttggcatc
     6541 aagaccctga agtatagatc cggcggcatg gatgagaaaa ccattgccct cctggtggct
     6601 ggcctggtca ttaccgtgat cgtgatcgtg ggcgctatcc tcttcgtccc tggcgagtac
     6661 agcctgaaaa acgccaccgg actgggactg atcgtgacct ccacaggaat cctgattctg
     6721 ctgcactatt acgtgtttag caccgctatc ggcctgacct cctttgtgat cgccatcctg
     6781 gtgatccagg tcatcgctta tatcctggcc gtcgtcggac tgagcctctg tatcgccgcc
     6841 tgtatcccca tgcatggccc cctcctcatc agcggactct ccattctggc cctggcccaa
     6901 ctcctgggcc tcgtgtacat gaagttcgtg gcctccaacc aaaaaacaat ccagcctccc
     6961 agaaaggccg tcgaggagcc cctgaacgct tttaaggaga gcaaaggcat gatgaacgat
     7021 gagggatccg gacagtgtac caactatgcc ctcctgaaac tggctggcga cgtggagagc
     7081 aatcctggac ctatgggcat ccagggcggc tccgtcctgt tcggcctcct cctcgtcctg
     7141 gccgtctttt gccattccgg ccattccctc cagtgctaca actgtcctaa ccccaccgcc
     7201 gactgtaaga cagctgtgaa ctgtagcagc gacttcgatg cttgcctgat cacaaaggct
     7261 ggactgcagg tgtacaataa gtgctggaag ttcgagcact gcaacttcaa tgacgtgaca
     7321 accaggctga gggaaaacga gctgacctac tactgctgca aaaaggacct gtgcaatttc
     7381 aatgagcagc tggagaatgg cggcacatcc ctgagcgaga aaaccgtgct gctgctggtg
     7441 acccctttcc tggccgccgc ttggtccctg caccccggct ccggagtgaa gcagaccctc
     7501 aacttcgatc tcctcaaact ggccggagac gtcgaaagca atcctggacc tatgatcgag
     7561 caggacggac tccatgctgg aagccctgcc gcttgggtcg agaggctctt tggatacgac
     7621 tgggctcagc agacaatcgg ctgcagcgat gccgccgtgt ttagactcag cgcccagggc
     7681 agacctgtgc tgtttgtgaa gaccgacctg agcggagctc tcaatgagct ccaagatgaa
     7741 gccgccagac tgagctggct cgccaccaca ggcgtgcctt gtgctgccgt gctggacgtc
     7801 gtcacagagg ccggaaggga ttggctgctc ctgggcgaag tccccggaca ggatctgctg
     7861 agctcccatc tggcccctgc cgaaaaggtc tccattatgg ccgatgccat gaggaggctg
     7921 cacaccctgg atcccgctac ctgtcccttt gaccatcagg ccaagcacag gattgagagg
     7981 gccagaacaa gaatggaggc cggcctggtc gaccaggatg atctggacga ggaacaccag
     8041 ggcctcgctc ctgccgagct ctttgccaga ctcaaggcca ggatgcctga cggcgaagat
     8101 ctggtggtga cccatggaga tgcctgtctc cccaacatca tggtggaaaa cggcaggttc
     8161 tccggattca ttgactgcgg caggctcggc gtggccgaca ggtatcagga catcgccctg
     8221 gctaccagag atatcgctga ggagctggga ggagagtggg ccgatagatt tctcgtgctc
     8281 tatggcattg ccgctcccga cagccagaga attgccttct acagactgct cgatgagttt
     8341 ttttgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     8401 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     8461 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     8521 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     8581 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     8641 cacgcgccct gtagcggcgc atctagcggc cgatcaggcc cgacctactc tcttccgcat
     8701 tggagtcgct ttaactggcc ctggctttgg cagcctgtgc tgacccatgc agtcctcctt
     8761 accatccctc cctcgacttc ccctcttccg atgttgagcc cctccagccg gtcctggact
     8821 ttgtctcctt ccctgccctg ccctctcctg aacctgagcc agctcccata gctcagtctg
     8881 gtctatctgc ctggccctgg ccattgtcac tttgcgctgc cctcctctcg cccccgagtg
     8941 cccttgctgt gccgccggaa ctctgccctc taacgctgcc gtctctctcc tgagtccgga
     9001 ccactttgag ctctactggc ttctgcgccg cctctggccc actgtttccc cttcccaggc
     9061 aggtcctgct ttctctgacc tgcattctct cccctgggcc tgtgccgctt tctgtctgca
     9121 gcttgtggcc tgggtcacct ctacggctgg cccagatcct tccctgccgc ctccttcagg
     9181 ttccgtcttc ctccactccc tcttcccctt gctctctgct gtgttgctgc ccaaggatgc
     9241 tctttccgga gcacttcctt ctcggcgctg caccacgtga tgtcctctga gcggatcctc
     9301 cccgtgtctg ggtcctctcc gggcatctct cctccctcac ccaaccccat gccgtcttca
     9361 ctcgctgggt tcccttttcc ttctccttct ggggcctgtg ccatctctcg tttcttagga
     9421 tggccttctc cgacggatgt ctcccttgcg tcccgcctcc ccttcttgta ggcctgcatc
     9481 aggccaaatt ggccgtcgac tgcagaggcc tgcatgcaag cttggcgtaa tcatggtcat
     9541 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9601 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9661 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     9721 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9781 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9841 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9901 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9961 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10021 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10081 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10141 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10201 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10261 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10321 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    10381 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10441 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10501 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10561 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10621 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    10681 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    10741 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10801 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10861 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10921 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10981 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    11041 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11101 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11161 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11221 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11281 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11341 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11401 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11461 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11521 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11581 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11641 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
